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Arief Prajitno
University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Uji Aktivitas Senyawa Antibakteri Ekstrak Aloe Vera (Aloe Vera) Terhadap Infeksi Bakteri Edwardsiella Tarda secara In Vitro Nuranti Anarkhis; Arief Prajitno; Maftuch Maftuch; Karimah Karimah
Jurnal Penelitian Pendidikan IPA Vol 9 No 2 (2023): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i2.2610

Abstract

Edwardsiella tarda is a type of gram-negative intracellular pathogen that can cause mass demise in fish farming. The development of edwarsiellosis due to E.tarda infection causes significant economic losses for cultivators. Aloe vera is a perennial succulent plant like a cactus, drought withstand, and belongs to the Liliaceae family, of which there are more than 360 known species (Wijaya and Masfufatun, 2022). Aloe vera is one of the plants known to contain active ingredients that can inhibit the growth of bacteria. The results of phytochemical analysis of a crude extract of Aloe vera showed the presence of active compounds, specifically flavonoids, tannins, triterpenoids, and saponins. MIC analysis showed that the lowest absorbance value of Aloe vera was obtained at a concentration of 50 mg/L with a value of 0.316, indicating a decrease at each concentration. The concentration of 50 mg/L is close to the positive control which indicates that at a concentration of 50 mg/L, it can be the reference for the lowest concentration in determining the dose of Aloe vera extract which is known that the presence of antibacterial compounds in Aloe vera extract can inhibit the growth of E.tarda bacteria. The inhibition zone test was carried out with graded concentrations of 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L with an observation time of 24 hours post-incubation and 48 hours post-incubation. Inhibition zone analysis with the highest diameter was at 200 mg/L. SEM analysis by distributing a crude extract of Aloe vera affected E.tarda bacteria specifically, lysis occurring in bacterial cells
Pengaruh Ekstrak Kasar Daun Keji Beling (Strobilanthes crispus) terhadap Infeksi Bakteri Aeromonas hydrophila Secara In Vitro Genesia Gerrine; Arief Prajitno; Mohamad Fadjar; Rahmalia Eka Kenitasari
Jurnal Penelitian Pendidikan IPA Vol 9 No 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3502

Abstract

Keji beling leaves (Strobilanthes crispus) can be the solution to cure the fish that infected by Aeromonas hydrophila because it contained active compounds. The purpose of this study was to determine the active compounds contained in the crude extract of keji beling leaves (S. crispus) and their ability to inhibit the growth of A. hydrophila. The tests which are used in this study were phytochemical test, MIC test, disk diffusion test, and SEM test. Phytochemicals analysis showed the presence of active compounds are flavonoid, alkaloid, tanin, and triterpenoid. The result of MIC test showed that concentration of 100 mg/l with a value of 0.389 was the lowest absorbance of the keji beling (S. crispus) crude leaves extract. The inhibition test used disk diffusin test with five concentrations of keji beling (S. crispus) crude leaves extract with three replications: 50 mg/l, 100 mg/l, 150 mg/l, 200 mg/l, and 250 mg/l, with the observation time of 24 hours post-incubation and 48 hours post-incubation. The concentration of 250 mg/l had the highest inhibition zone. The result of SEM analysis showed that keji beling (S. crispus) crude leaves extract can damage the cell walls of A. hydrophila and lysis the bacterial cells.
Co-Authors Genesia Gerrine Karimah Karimah Maftuch Maftuch Mohamad Fadjar Nuranti Anarkhis Rahmalia Eka Kenitasari