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All Journal Biotika: Jurnal Ilmiah Biologi Jurnal Penelitian Tanaman Industri Jurnal Matematika Sains dan Teknologi Jurnal Mikologi Indonesia Journal of Science Innovare FITOFARMAKA: Jurnal Ilmiah Farmasi EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Jurnal Penelitian Tanaman Industri (Littri)
Tri Saptari Haryani
Pakuan University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Other

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POTENSI RUMPUT LAUT DI PANTAI BAYAH, KABUPATEN LEBAK, BANTEN SEBAGAI ANTIBAKTERI Escherichia coli Triastinurmiatiningsih, Triastinurmiatiningsih; Haryani, Tri Saptari
Jurnal Matematika Sains dan Teknologi Vol 9 No 1 (2008)
Publisher : LPPM Universitas Terbuka

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Abstract

Antibacterial potency of some seaweed against Escherichia coli has been known. The aim of this research is to know the species of seaweeds from Bayah beach Lebak Banten which may be used as antibacterial against Escherichia coli. Twenty one seaweed species samples from Bayah Beach Lebak Banten were exstracted by organic solvent of absolute methanol according to Espeche, Fraile, and Mayer (1984) and Darusman, Sayuti, Komar & Pamungkas (1992) methods. The antibacterial activities were examined by means of Kirby-Bauer or diffusion method. The results showed antibacterial activities occur from the extract of Boodlea composite, Chaetomorpha crassa, Ulva fasciata, Sargassum crustaefolium, Padina australis and Halimeda gracilis. Sofar, Padina australis extract had shown better inhibitory activities than others.
EFEKTIVITAS EKSTRAK Padina australis SEBAGAI ANTIBAKTERI Escherichia coli PENYEBAB DIARE Saptari Haryani, Tri; Lohita Sari, Bina; Triastinurmiatiningsih, Triastinurmiatiningsih
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 4, No 2 (2014): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

Organisme laut merupakan sumber senyawa obat yang berpotensi besar, tetapi sampaisaat ini masih belum dimanfaatkan secara maksimal. Penelitian efektivitas ekstrak Padina 
EFEKTIVITAS EKSTRAK Padina australis SEBAGAI ANTIBAKTERI Vibrio cholerae DAN Salmonella typhi Haryani, Tri Saptari; Triastinurmiatiningsih, -; Ardiani, Weni
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

The current utilization of natural materials as a traditional medicine in Indonesia. Aquatic plants has potential as a traditional medicine because it contains active ingredients that are good for health, one of them namely, Padina australis Hauck.The purpose of this study is to establish the most effective concentration of extract of Padina australis as Vibrio cholerae antibacterial and Salmonella typhi. Extract of Padina australis is made by maceration method. Test of the effectivity of extract of Padina australis against Vibrio cholerae and Salmonella typhi. Disc diffusion method Kirby Bauer at the treatment the extract concentration of 50%, 60%, 70%, 80% and control. Results of testing the extract Padina australis against Vibrio cholerae showed a concentration of 80% is the concentration forming the greatest drag areas namely 19.48 mm, while the test results against Salmonella typhi bacteria extract at concentrations of 80% obtained a diameter drag area of 12 mm. Key words : antibacterial, Padina australis Hauck, Vibrio cholerae, and Salmonella typhi.
KEANEKARAGAMAN DAN POTENSI PAKU (PTERIDOPHYTA) DI TAMAN NASIONAL GUNUNG GEDE PANGRANGO CIANJUR (TNGGP) Syafrudin, Yudi; Haryani, Tri Saptari; Wiedarti, Sri
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

The research Diversity and Potential paku (Pteridophyta)  in The Taman Nasional Gede Pangrango Montain Cianjur have been done. Transek and plot sample method use in this research on three area of different altitude (1600, 1900, 2200 m above sea level). The result research show there were 56 species of ferns that include in 21 families. At an altitude of 1600 m above sea level diversity ferns include in the medium category with value 1,48, at an altitude of 1900 m above sea level diversity ferns include in the medium category with value 1,02, and altitude of 2200 m above sea level diversity ferns include in the low category with value 0,93. Ferns in the TNGGP have potensial for the surroundings communities  Kebun Raya Cibodas. Key words : Ferns, diversity, Gede Pangrango Mountain.
KEANEKARAGAMAN JENIS JAMUR DI TAMAN WISATA ALAM SITUGUNUNG, CISAAT, SUKABUMI Triastinurmiatiningsih, -; Haryani, Tri Saptari; Tampubolon, Jureka
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

Some of the various flora that Indonesia has is the diversity of fungi, lichens and moss. Taman natural  tourism Situgunung is one place that is suitable for mushrooms. The study was conducted at an altitude of 950 m above sea level, 990 m above sea level and 1050 m above sea level. Each height is made of three transect lines with a length of each 100 m transect with a total plot of 90 plot samples. Each transect is made of 10 sample plots measuring 1 x 1 m with a spacing between 10 m. The important value index (INP) that dominates each height is Marasmius copelandi, the height of 990 m above sea level is Stereum ostrea, and the Important Value Index at an altitude of 1050 is Stereum sp. Diversity index of fungal species in the TWA Situgunung area is high with an average value of 3.78.Key words : Situgunung, fungi, index diversity
PEMANFAATAN BAKTERI ANTAGONIS TERHADAP PENGENDALIAN JAMUR PATOGEN Fusarium oxysporum dan Phytopthora capsici SECARA IN VITRO Haryani, Tri Saptari; Tombe, Olivia Mersylia
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

Jamur Fusarium oxysporum merupakan patogen yang dapat menyebabkan penyakit busuk batang pada tanaman vanili. Adanya pembusukan pada jaringan batang tersebut merupakan ciri khas dari penyakit busuk batang. Gejala khas dari serangan patogen pada pangkal batang mengakibatkan pangkal batang menjadi berwarna hitam. Salah satu cara untuk mengendalikannya yaitu menggunakan agen pengendali hayati berupa bakteri antagonis. Tujuan dari penelitian ini untuk mendapatkan satu isolat bakteri antagonis yang paling efektif menekan pertumbuhan koloni serta perkecambahan konidia dan zoospora  patogen Fusarium oxysporum dan Phytopthora capsici. Penelitian dilakukan secara in vitro, tahapan kegiatan dalam penelitian ini meliputi sterilisasi alat, pembuatan media, pembuatan inokulum, pengujian antagonis isolat bakteri dan filtratnya serta pengamatannya. Bahan yang digunakan adalah isolat F.oxysporum, P.capsici dan sembilan isolat bakteri. Hasil penelitian menunjukkan bahwa dari sembilan isolat bakteri terdapat satu isolat bakteri paling efektif menghambat pertumbuhan patogen F.oxysporum yaitu isolat bakteri AKT7 sebesar 29,91% sedang isolat bakteri paling efektif menghambat patogen P.capsici yaitu isolat bakteri B5A sebesar 54,42% . Filtrat bakteri AKT7 mampu menghambat perkecambahan konidia F.oxysporum dan zoospora P.capsici. Kata kunci :  Fusarium oxysporum ,  Phytopthora capsici, bakteri antagonis
Isolation And Identification Of Pathogen Mushroom Types On Umbi Talas (Colocasia Esculenta (L.) Schoot) Post Harvest Sulistiyono, Fitria Dewi; Haryani, Tri Saptari
Journal of Science Innovare Vol 1, No 01 (2018): Journal of Science Innovare
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.273 KB) | DOI: 10.33751/jsi.v1i01.685

Abstract

The taro tuber (Colocasia esculenta) is an important source of carbohydrates for an important energy producer whose portion has a fairly bright and profitable development prospect. Taro is not only used as a food source, it can be used for industrial purposes, for example raw materials of cosmetics and plastic. Taro is very easy to recognize and varied with other preparations because it has a distinctive flavor. Increased production is the only major consideration in taro cultivation. Efforts to increase production are influenced by limiting factors that are often experienced in the community. Such barrier is a pathogen attack both on the ground and post harvest. The purpose of this study is to isolate and identify the types of pathogenic fungi found in taro tubers. The method used is microscopic and macroscopic identifications. The results of identification with the macroscopic and microscopic observations obtained by the genus Aspergillus, Candida, Sclerotium, Fusarium, Mucor, and Rhizopus. Keywords: Taro, Identification, Pathogenic Fungi
POTENSI RUMPUT LAUT DI PANTAI BAYAH, KABUPATEN LEBAK, BANTEN SEBAGAI ANTIBAKTERI Escherichia Coli Triastinurmiatiningsih Triastinurmiatiningsih; Tri Saptari Haryani
Jurnal Matematika Sains dan Teknologi Vol. 9 No. 1 (2008)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.16 KB)

Abstract

Antibacterial potency of some seaweed against Escherichia coli has been known. The aim of this research is to know the species of seaweeds from Bayah beach Lebak Banten which may be used as antibacterial against Escherichia coli. Twenty one seaweed species samples from Bayah Beach Lebak Banten were exstracted by organic solvent of absolute methanol according to Espeche, Fraile, and Mayer (1984) and Darusman, Sayuti, Komar & Pamungkas (1992) methods. The antibacterial activities were examined by means of Kirby-Bauer or diffusion method. The results showed antibacterial activities occur from the extract of Boodlea composite, Chaetomorpha crassa, Ulva fasciata, Sargassum crustaefolium, Padina australis and Halimeda gracilis. Sofar, Padina australis extract had shown better inhibitory activities than others.
KEANEKARAGAMAN DAN KELIMPAHAN MAKROALGA DAN TUMBUHAN MANGROVE DI KAWASAN GEOPARK, CILETUH, PELABUHAN RATU, KABUPATEN SUKABUMI Tri Saptari Haryani; Triastinurmiatiningsih Triastinurmiatiningsih; Dede Giwang Maelani; Zulkhoir Zulkhoir
BIOTIKA Jurnal Ilmiah Biologi Vol 18, No 2 (2020): BIOTIKA DESEMBER 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v18i2.31034

Abstract

Ciletuh merupakan salah satu kawasan ekowisata yang menerapkan pendekatan based tourism yaitu masyarakat berperan penting dalam menunjang pembangunan pariwisata. Makroalga dan tumbuhan mangrove merupakan sumberdaya hayati yang memiliki peranan penting secara ekologi, terutama karena daya dukungnya bagi kawasan pesisir, serta sebagai sumber makanan dan habitat bagi berbagai biota laut, Penelitian bertujuan untuk mengidentifikasi jenis-jenis, keanekaragaman dan kelimpahan makroalga dan tumbuhan mangrove di kawasan Geopark Ciletuh, Kabupaten Sukabumi. Pengambilan sampel menggunakan metode line transect dilakukan di pantai Cikadal, Mandrajaya. Keanekaragaman makroalga dan mangrove dianalisis berdasarkan perhitungan indeks keanekaragaman dan kelimpahan relatif. Hasil penelitian diperoleh makroalga sebanyak 25 spesies yang terbagi ke dalam 12 famili, dan tumbuhan mangrove diperoleh sebanyak 13 spesies terbagi ke dalam 5 famili.  Indeks Keanekaragaman jenis makroalga didominasi oleh jenis Gracillaria gracilis dengan nila Indeks Kelimpahan Relatif (IKR) sebesar 3,34, dan tumbuhan mangrove didominasi oleh jenis Avicennia officinalis dengan nilai IKR sebesar 2,89. Hal ini menunjukkan bahwa wilayah Geopark Ciletuk masih didominasi oleh makroalga, khususnya jenis Gracillaria gracilis (Famili gracillariaceae). Kesimpulan penelitian menunjukkan bahwa keanekaragaman makroalga tergolong tinggi dan kenaekaragaman tumbuhan mangrove tergolong rendah, sehingga diperlukan pengendalian terhadap biota laut akibat aktivitas manusia.
KEEFEKTIFAN FORMULA MINYAK CENGKEH DAN SERAI WANGI TERHADAP Fusarium oxysporum f.sp. vanillae PENYEBAB BUSUK BATANG VANILI MESAK TOMBE; DARMAWAN PANGERAN; TRI SAPTARI HARYANI
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.143-150

Abstract

ABSTRAKBusuk batang vanili (BBV) yang disebabkan oleh Fusariumoxysporum f.sp. vanillae (Fov) merupakan patogen penting pada vanili danmenyebabkan kerugian besar setiap tahunnya di Indonesia. Jumlahkerugian diperkirakan sebesar 3.000 ton atau sekitar US$ 16 juta.Penelitian dilaksanakan di laboratorium dan rumah kaca Kelti Hama danPenyakit, Balai Penelitian Tanaman Rempah dan Obat, Bogor pada bulanFebruari 2010 sampai Agustus 2010. Tujuan penelitian adalah untukmengetahui pengaruh formula dengan bahan aktif minyak cengkeh danserai wangi terhadap penyakit BBV dan dampaknya terhadap kehidupanmikroorganisme tanah. Dalam pelaksanaannya, kegiatan penelitian dilaku-kan dalam 3 tahap yaitu : (1) uji formula in vitro, (2) uji formula in vivo,dan (3) uji dampak formula terhadap mikroorganisme tanah. Percobaantahap 1, 2, dan 3 masing-masing terdiri atas 10, 6, dan 6 perlakuan,dan.masing-masing disusun menggunakan rancangan acak lengkap dengan3 ulangan. Materi yang dikaji dalam penelitian ini adalah (1) formuladengan bahan aktif minyak cengkeh (formula standar), (2) formula denganbahan aktif minyak cengkeh dan serai wangi (formula baru), dan (3)fungisida sintetis berbahan aktif mankozeb sebagai pembanding. Hasil ujiin vitro menunjukkan bahwa fungisida nabati CS (minyak cengkeh + seraiwangi) pada ke 3 tingkatan konsentrasi (terutama konsentrasi 400 ppm)memperlihatkan efektivitas yang tinggi dalam menghambat pertumbuhanmiselium dan produksi spora patogen Fov.F117 dibandingkan perlakuanlain dan kontrol. Hasil uji in vivo menunjukkan hal yang sama bahwafungisida nabati CS memperlihatkan efektivitas yang tinggi dalammenekan intensitas serangan patogen BBV, terutama pada dosis aplikasi 5ml/l. Dampak perlakuan terhadap kehidupan mikroorganisme tanah (fungidan bakteri) memperlihatkan bahwa penggunaan fungisida sintetismankozeb menghambat kehidupan mikroorganisme tanah 90-100% jikadibandingkan dengan kontrol. Populasi mikroorganisme tanah pada semuaperlakuan fungisida nabati lebih tinggi jika dibandingkan dengan fungisidamankozeb. Populasi mikroorganisme pada aplikasi fungisida nabati padadosis 5 ml/l tidak berbeda nyata dengan kontrol. Penelitian inimenunjukkan bahwa penggunaan fungisida nabati minyak cengkehterutama jika dikombinasikan dengan minyak serai wangi mempunyaiprospek untuk digunakan dalam pengendalian penyakit BBV denganinterval aplikasi 3-4 minggu sekali.Kata kunci : Vanilla planifolia, Fusarium oxysporum f.sp, vanillae, busukbatang vanili, minyak cengkeh, minyak serai wangiABSTRACTVanilla stem rot (VSR) disease caused by Fusarium oxysporum f.sp.vanillae (Fov) is an important disease on vanilla and causes severe lossannually in Indonesia. The total annual loss in production due to the VSRwas estimated to be 3,000 ton or about US$ 16 million. This research wascarried out in the laboratory and green house of Pest and Disease ofResearch Institute for Spices and Medicinal Crops, Bogor from February2010 until August 2010. The objective of this research was to study theeffect of formula using active compounds of clove and citronella oils onthe VSR disease and its impact on the survival of soil microorganisms.The research activities were conducted in three stages, namely (1) in vitroformula test; (2) in vivo formula test, and (3) impact test of formula on thesoil microorganisms. Materials studied in this research were (1) formulawith active compound of clove oil (standard), (2) formula with activecompound of clove and citronella oils (new formula), and (3) syntheticfungicide with mancozeb active ingredient as comparison. Every phase ofthis 3 stage experiment was arranged using completely randomized designwith three replicates. First, second, and third phases of the experimentconsisted of 10, 6, and 6 treatments, respectively. In vitro test resultscarried out in the laboratory indicated that botanical formula CS (clove andcitronella oils of the three concentration levels, especially on the 400 ppm)showed high effectiveness on inhibiting mycelium growth and sporeproduction of Fov.F117 pathogen compared to other treatments andcontrol. In vivo test in the green house indicated the same result thatbotanical fungicide CS (clove dan citronella oils) showed higheffectiveness in suppressing VSR disease infection intensity, especially on5 ml/l dosage. Application of mancozeb synthetic fungicide inhibited 90 –100% soil microorganism livelihood compared to control. However, thesoil microorganism population on all botanical fungicides was higher thanon mancozeb fungicide. The microorganism population on the botanicalfungicide of 5 ml/l dosage was not significantly different from the control(natural soil with no treatment). This research indicates that aplication ofbotanical fungicide (combination between clove and citronella oils) with3 - 4 weeks interval is prospectous to control VSR disease.Key words : Vanilla planifolia, Fusarium oxysporum, f.sp. vanillae,vanilla stem rot, clove oil, citronella oil
Co-Authors Ade Kusmiati Ardiani, Weni Bina Lohita Sari DARMAWAN PANGERAN Dede Giwang Maelani Dina Agustina Fiona Azzahro Fitria Dewi Sulistiyono Ghina Athyah Wahid Indah Nur Sayyidah Intan Lestari MESAK TOMBE Oom Komala Siti Suryani Tahier Sri Wiedarti Syafrudin, Yudi Tampubolon, Jureka Tombe, Olivia Mersylia Triastinurmiatiningsih Triastinurmiatiningsih Triastinurmiatiningsih, - Triastinurmiatiningsih, Triastinurmiatiningsih Uswatun Hasanah Yulianti Bramasto Zulkhoir Zulkhoir