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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal Penelitian Pendidikan IPA (JPPIPA)
Sri Andayani
University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics

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 1 
Antibacterial Ability of Rhizophora mucronata Leaf Extract Against Bacterial Infections of Edwardsiella tarda Ramanda Ahmad Rizal Rifa'i; Sri Andayani; Mohamad Fadjar
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.02

Abstract

Edwardseilla tarda is a group of gram-negative bacteria with facultative anaerobic properties that can infect both cultivated and wild fish. Edwardseilla tarda infection causes considerable economic losses in the world. Rhizopora mucronata is a plant that is known to have the ability to produce secondary metabolites such as alkaloids and flavonoids. This study aims to determine the ability of R. mucronata to inhibit the growth of E. tarda as indicated by the presence of an inhibition zone and the absorbance value in the tube dilution test. The method used in this study is the disc diffusion test and the tube dilution test. The results of the phytochemical analysis showed the presence of flavonoid and alkaloid compounds in the crude extract of R. mucronata leaves. A concentration of 15.6 mg.L-1 showed a minimal inhibitory response in inhibiting the growth of E. tarda bacteria. In comparison, the concentration of 56 mg.L-1 showed the highest inhibitory response in inhibiting the growth of E. tarda bacteria. It indicates that the compounds contained in R. mucronata can be used as alternative ingredients in medicine to treat E. tarda bacterial infections. Keywords: Alkaloid, Antibacterial, E. tarda, Flavonoid, R. mucronata.
Latent and Eggs Production of Banggai Cardinal (Pterapogon kauderni, Koumans 1933) on Various Salinity Levels: Conservation Efforts Atiek Pietoyo; Sri Andayani; Agoes Suprijanto
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (867.267 KB) | DOI: 10.21776/ub.jels.2016.006.01.05

Abstract

The aim of this study is to determine the best salinity level on latent and eggs production of Banggai Cardinal (Pterapogon kauderni Koumans 1993) based on first brood and brood production of Banggai Cardinal in the treatment. Water quality measurement was carried out every day to maintain water quality. Water quality measurement showed suitable salinity for Banggai Cardinal breeding. Brood latent showed no significant difference between the treatments. Total of 27 ppt is the level of salinity for Banggai Cardinal reproduction to gain positive respond on eggs production (42.3333 ± 7 eggs). Keywords: Banggai Cardinal, Eggs Production, Latent, Salinity.
The Alteration Tissue Structure of Digestive diverticulum of Anodonta woodiana Induced by Lead Renanda Baghaz Dzulhamdhani Surya Putra; Sri Andayani; Hartati Kartika Ningsih; Diana Arfiati; Asus Maizar Suryanto Hertika
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1617.255 KB) | DOI: 10.21776/ub.jels.2018.008.02.03

Abstract

Accumulated the toxic compound such as lead in A. woodiana caused an alteration in tissue. The aim of the present study is observation through histology to determine the alteration of the structure of digestive diverticulum tissue. A. woodiana were maintained in water tub that treated lead II nitrate exposure (Pb(NO3)2) 0, 15, 25, 35 mgL-1 for 7 days to determine the alteration of the structure of damaged the digestive diverticulum and determining water quality such temperature, DO and pH. The highest damage occurs in digestive diverticulum treated 35 mgL-1  due to the many alteration tissue structure such as hyperplasia, edema, atrophy, necrosis with the percentage of total damage of about 35%. the lowest alteration tissue structure occurs in 15 mgL-1 treatment group of the digestive diverticulum. the temperature was observed at about 25.3 - 26.4°C, pH about 6-7, and DO about 6.1-6.7 mgL-1. Thus, the increment of lead dose treatment induces increment of tissue structure alteration in digestive diverticula tissue, therefore, might promote the death of Anodonta woodiana. Keywords: Anodonta woodiana, digestive diverticulum, histology
Perbedaan Padat Penebaran Terhadap Kualitas Air Ikan Nila Merah (Oreochormis sp) Pada Sistem Budikdamber Vina Nur Nadiro; Sri Andayani; Maheno Sri Widodo; Nurhalisa Nurhalisa
Jurnal Penelitian Pendidikan IPA Vol 9 No 4 (2023): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i4.3236

Abstract

The problems of fish and plant cultivation are limited land, decreasing quality and quantity of water for human needs, food sources, and increasing population on earth. Optimization of fish farming with high stocking densities accompanied by high feeding will cause accumulation of organic matter in the culture containers which will worsen the quality of the rearing water which will ultimately have an impact on the physiological conditions, survival and growth of fish. One of the technologies for growing vegetables, fruit and fish farming that has been developed to overcome these obstacles is the Budikdamber system with red tilapia organisms and water spinach plants. The purpose of this study was to determine the quality of water with different stocking densities in the Budikdamber system with water spinach and without water spinach. The experimental design used a factorial complete randomized design with density treatment (A (2 fish/10L), B (4 fish/10L), C (6 fish/10L), D(8 fish/10L) and system (budikdamber with water spinach) (a) and without water spinach (b)). The results showed that temperature parameters ranged from 22.4 - 30.6°C, pH ranged from 6.4 - 8.9, and DO ranged from 1.07 - 16.4 mg/l. Parameters of temperature, pH, and DO are in a range that is not in accordance with optimum conditions (temperature = 25-32°C, pH = 6.5 – 8, and DO ≥3 ), but can still be tolerated by tilapia so that fish tilapia still survive. Water spinach plants can reduce ammonia so that the ammonia value in the treatment using kale decreased from week 1 to 4 and conversely, for budikdamber without water spinach, it increased from week 1 to week 4. The highest SR value was found in the Ba treatment (4 fish/10 liters) with water spinach is 91.67%.
Fish Growth Performance in RAS Pond Using Hydrocyclone Mechanical Filter Aulia Rahmawati; Febriyani Eka Supriatin; Sri Andayani; Muhammad Nasim Mubarok; Aulia Rahman
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.6149

Abstract

The high feeding and the metabolic output of the fish in intensive aquaculture can cause various problem, such as poor water quality and slowing down the fish growth performance. Recirculating Aquaculture System (RAS) is a solution to maintain water quality. Unfortunately, the conventional mechanical filter in RAS such as japmat is inefficient. One strategy that can be applied is to use a hydrocyclone. This research aims to observe the fish growth performance in RAS pond using hydrocyclone mechanical filter and its comparison with the use of japmat. Research method was performed by comparing hydrocyclone and japmat in the pond with the RAS system. The research design is completely randomized design (CRD), with three replications in each treatment. The research was carried out through five stages, namely hydrocyclone set-up, RAS ponds set-up, water quality analysis, fish growth measurement, and statistical analysis. Based on the Independent Samples Test, the use of hydrocyclone mechanical filters has a significant effect (P<0.05) on the measured water quality parameters (DO, Temperature, pH, TDS, NH3) except for NO2. Hydrocyclone significantly affect Average Daily Growth and Specific Daily Growth parameters. Whilst, the Survival Rate and Feed Conversion Ratio parameters, it did not give significant effect
Co-Authors Agoes Suprijanto Asus Maizar Suryanto Hertika Atiek Pietoyo Aulia Rahman Aulia Rahmawati Diana Arfiati Hartati Kartika Ningsih Maheno Sri Widodo, Maheno Sri Mohamad Fadjar Muhammad Nasim Mubarok Nurhalisa Nurhalisa Ramanda Ahmad Rizal Rifa'i Renanda Baghaz Dzulhamdhani Surya Putra Supriatin, Febriyani Eka Vina Nur Nadiro