Tjok Gde Oka Pemayun
Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia

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Penyuntikan Gonadorelin pada Saat Estrus Terhadap Perkembangan Folikel dan Terjadinya Ovulasi serta Non Return Rate pada Sapi Bali yang Mengalami Kawin Berulang Gusde Wahyu Krisna Suputra; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun; I Wayan Sukernayasa; I Nyoman Oka Widiarta
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p11

Abstract

This study aims to prove that gonadorelin administration is able to cause follicle development to reach follicles that are ready to be ovulated and ovulation and fertilization occurs in bali cattle that experience repeated breeder in Sobangan Village, Badung Regency, Bali. Observations of developing follicles and the timing of ovulation were performed using transrectal ultrasound via rectal palpation. The design used in this study was a completely randomized design (CRD) consisting of three treatment groups, namely the control group without gonadorelin injection (P0), the group receiving 50 g/im/head injection of gonadorelin (P1) and the group receiving 50g/im/head injection. gonadorelin injection at a dose of 100g/im/head (P2). Gonadorelin injection was performed when clear discharge came out of the vagina as a sign of the appearance of estrus and all samples in IB at 12 hours after the appearance of estrus. The results showed that the average follicle diameter at the time of estrus in the P0 group was 7.37+0.46mm, the P1 group was 7.67+0.78mm, and the P2 group was 7.59+0.77mm and statistically did not show any difference. which meaningful (P> 0.05). When reaching the de graff follicle, the mean follicle diameter in the P0 group was 11.27+0.59mm, the P1 group was 10.08+0.60mm, the P2 group was 9.92+1.06mm statistically showed a significant difference (P<0.05). At ovulation the mean follicle diameter in the P0 group was 10.53+0.57mm, in the P1 group 9.51+0.59mm, in the P2 group 9.33+0.89mm, statistically showed a significant difference (P<0.05). Ovulation time in the P0 group was 71.56+1.33 hours, while for the P1 and P2 groups it was 12.00+0.00 hours and statistically showed a significant difference (P<0.05). The percentage of non-return rates in the three treatment groups were 0%, 77.8% and 88.9%, for P0, P1 and P2, respectively.
Penambahan Vitamin E pada Pengencer SemenLife® Terhadap Kualitas dan Lama Simpan Spermatozoa Babi Landrace Gede Putra Sanjaya; Wayan Bebas; Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p16

Abstract

Artificial insemination in pigs in Indonesia still uses liquid semen. Therefore, specific media are needed to maintain the quality of pig spermatozoa. This study aims to determine the effect of adding vitamin E (a-tocopherol) to Semenlife® (INTTEC) diluent on the quality and shelf life of spermatozoa of landrace pigs. The sample used was fresh semen which was collected using a mass technique taken from the UPTD. Regional Artificial Insemination Center, Livestock Breeding and Forage/Livestock Institute of Bali Province. A total of 24 samples consisting of 12 samples in the SemenLife® (INTTEC) diluent and 12 samples in the SemenLife® (INTTEC) diluent + vitamin E 400 µg/ml were stored at 15-18oC. Observation of spermatozoa quality (viability and motility) and storage time was carried out for 120 hours with observations of spermatozoa quality under a microscope every 24 hours, then DNA fragmentation was tested using the Pocrine 8-Hydroxy-desoxyguanosine ELISA Kit. The graph results of Duncan's test showed that the addition of 400 µg/ml vitamin E had a significant effect on the quality of spermatozoa, especially in terms of motility which was maintained at 48 hours and the lowest DNA fragmentation occurred at 72 hours. Furthermore, the addition of vitamin E was not able to extend the shelf life of landrace pig spermatozoa in diluent. Where, there was a decrease in motility and an increase in DNA fragmentation, respectively at 72 and 96 hours, in viability the decrease occurred periodically from the 0 hour to 120 hours. From the results of the quality and shelf-life tests obtained, spermatozoa can then be tested by conducting a fertility test to find out whether the results obtained can increase litter size.