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All Journal The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Research Journal of Life Science Indonesian Aquaculture Journal Jurnal Riset Akuakultur Journal of Aquaculture and Fish Health Journal Of Aquaculture Development and Environment

Agoes Soeprijanto, Agoes

Brawijaya University

Author-ID : 5918433

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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The Quality of Ornamental Japanese Carp (Cyprinus carpio var. Koi) After Electroporation as a Gene Material Transfer Method Saidah Luthfiyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Spermatozoa are male gametes that have an advantage in acting as gene transfer media (SMGT), because in transferring genetic material, sperm uses relatively natural vectors. This study was conducted to know sperm quality of Koi after short electroporation with different voltages using Gene Pulser. The study was conducted using a complete randomized design (RAL) with three (3) replications and five (5) treatments, namely: 10 V.cm-1, 20 V.cm-1, 30 V.cm-1, 40 V.cm-1, and 1 control. Electroporation was done with square wave, 1 ms duration of electroporation, 4 times of electroporation shocks with 0.1 s duration. The results showed that electroporation with different voltage levels significantly affected the motility and viability of sperm. Based on orthogonal polynomial analysis, it was known that the treatment affected motility and viability of sperm with a linear equation y = -0.9x + 5, 4 R² = 0.920 and r = 0.96 for motility and y = -11.26x + 91.61 R² = 0.823 and r = 0.91 for viability, meaning that the higher the level of electroporation the lower the viability and sperm motility produced. From the results of this study, it can be suggested that the electroporation of Koi sperm as a gene transfer medium should be performed at a voltage of 10 V.cm-1. Keywords: electroporation, Koi, sperm quality, transgenesis.

The Effect of Electroporation Method towards the Motility and Viability of Java Barb Fish (Puntius javanicus) Sperm Dimas Adetia Rikianto; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Electric shock treatment of Java Barb Fish (Puntius javanicus) sperm using electroporation method on sperm as transfer gen (Sperm Mediated Gen Transfer) has not been implemented in Indonesia. This study was conducted to know the effect of electric shock using gene pulser at different voltage level toward motility and viability of Java barb fish sperm. This research was conducted at Fish Breeding Laboratory, Faculty of Marine and Fisheries and LSIH of Brawijaya University-Malang in May 2017. The trial design used Complete Randomized Design with 3 different treatments and 1 control which each treatment repeated 3 times. The treatments used the Gene pulser with 3 different voltages: A (20 V), B (30 V), C (40 V). The result showed that the electric shock treatment with different voltages level affected motility and viability of Java barb fish sperm. Based on the data analysis used polynomial orthogonal, a linear-form of the relationship among the treatments in the form of equation was found (Puntius javanicus) with R2=0.9815 and equation y = -40.5x + 130.83 with R2= 0.8626. Based on the result of this research, electroporation for Java barb fish' sperm as gene transfer media should be done with voltage 20 V, pulse number 4 times and pulse length 1 ms. Keywords: electroporation, motility, Puntius javanicus, sperm, viability.

Effect of 17β-Estradiol on Feminization, Growth Rate and Survival Rate of Pacific White Shrimp (Litopenaeus vannamei, Boone 1931) Postlarvae I Nengah Gde Sugestya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

This study was therefore aimed to determine the effect of different concentration of estrogen hormone, 17β-estradiol (E2) on feminization for the production of all female, growth rate and survival rate of Pacific white shrimp (Litopenaeus vannamei) postlarvae (PL). L. vannamei PL1 were stocked randomly at a density of 10 ind.L-1 into a 10 L of seawater for each experimental flask with three replicates each. PL1 was immersed in seawater containing 0.5, 1.0 and 1.5 mg.L-1 estrogen hormone, E2 for 4 hours. The experiment was continued for 30 days for larval rearing. At the termination of experiment, the specimens in each treatment groups were weighed and measured individually for their wet body weight (BW) and total length (TL) for the mean weight gain, length gain, and the specific growth rate (SGR) estimation. The mean sex ratio values of male to female postlarvae obtained from control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 1:1, 1:2, 1:9 and 0:1 respectively. The weight gain and length gain were 40605% and 606% for control, 46310% and 647% for 0.5 mg.L-1 concentration, 49310% and 663% for 1.0 mg.L-1 concentration and 45048% and 628% for 1.5 mg.L-1. The mean SGR BW and SGR TL for control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 20.00 and 6.51, 20.46 and 6.70, 20.67 and 6.77 and 20.37 and 6.62 respectively. The mean survival rate for E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 52.67%, 54.33%, 54.67% and 52.33% respectively. The study shows that while the concentration of E2 hormone increased, the female sex ratio of L. vannamei PL also increased but the growth rate and survival rate decreased at concentration of 1.5 mg.L-1. Thus the optimum concentration of this hormone usage was 1.0 mg.L-1 immersed for 4 hours. Keywords: 17β-estradiol, feminization, growth rate, Litopenaeus vannamei, postlarvae, survival rate.

The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) Harun Wijaya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.

Influence of Different Pulse Length towards Motility and Viability of Ornamental Japanese Carp (Cyprinus carpio Var. Koi) Sperm through Electroporation Method Diana Aisyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.007.01.06

Successful studies about implementation of electroporation method which sperm becomes gene transfer medium for fish transgenesis have reported. Motility and viability are two major factors in successful of electroporation. The objective of the study was to determine optimal pulse length of electroporation towards the motility and viability of Koi fish sperm. The study was conducted at the Central Laboratory of Biological Sciences and Reproduction of Brawijaya University. The data analysis was conducted using completely randomized design. The electroporation method was carried out using voltage of 30 volt with pulse number of 4 times. The pulse length of the electroporation (based on treatment) was 0.5, 1 and 1.5 ms. The result showed the highest percentage of motility was 3.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 0.9643 and the best percentage of viability was 79.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 1. Correlation between the pulse length treatment (electroporation) toward motility and viability of Koi fish sperm, in which longer duration of pulse length treatment (electroporation) would decrease the motility and viability percentage of the fish sperm.Keywords: Electroporation, Motility, Pulse length, Sperm, Viability.

Co-Authors Abdul Wafi Diana Aisyah Dimas Adetia Rikianto Emir Ma’arif Imanudin Harun Wijaya Heri Ariadi I Nengah Gde Sugestya Immanuel A.H. Pandaingan Jojo Subagja Kurniawan Kurniawan Maheno Sri Widodo, Maheno Sri Muhammad Fakhri Otong Zenal Arifin Pratama Deffi Samuel R Adharyan Islamy Saidah Luthfiyah Sholikah, Lik Anatus Toni Yulian Kusmardani Yuni Kilawati Yunita Maemunah Yunita Maemunah Yunita Maimunah

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