Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal The Journal of Pure and Applied Chemistry Research
Marsha Anggita Amelia
Program of Pharmacy, Faculty of Science and Technology, Ma Chung University
Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Medicine & Pharmacology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Potential Cytotoxic Activity of Methanol Extract, Ethyl Acetate, and n-Hexane Fraction from Clitoria ternatea L. on MCF-7 Breast Cancer Cell Line and Molecular Docking Study to P53 Rollando Rollando; Marsha Anggita Amelia; Muhammad Hilmi Afthoni; Kestrilia Rega Prilianti
The Journal of Pure and Applied Chemistry Research Vol 12, No 1 (2023): Edition January-April 2023
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2023.012.01.705

Abstract

Breast cancer is a condition where the cells in breast tissue lose control and multiply uncontrollably. In this study, MCF-7 breast cancer cells were tested for cytotoxic activity using the MTT assay and the active compound's interaction with the p53 protein was tested in silico. The most active fraction was found to be the ethyl acetate fraction, with an IC50 value of 1.730 μg/mL and a selectivity index of 2.485. However, the selectivity index was less than 3, and Vero cells showed changes in morphology with the addition of the ethyl acetate fraction. GC-MS was used to identify 19 compounds in the ethyl acetate fraction, and in-silico tests were performed on 5 potential anticancer compounds. Lipinski's Rule of Five test showed that only 3 of these compounds could undergo molecular docking. The results indicated that Anethole compound can interact with p53 protein, while Cinnamaldehyde, (E)- can interact with p21 protein.
Co-Authors Kestrilia Rega Prilianti Muhammad Hilmi Afthoni Rollando, Rollando