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Aspek Molekuler Hubungan Asupan Zinc dan Selenium dengan Hemoglobin Glikosilasi pada Pasien Diabetes Mellitus Tipe 2 Yuliati Widiastuti, Indranila Kustarini Samsuria Judiono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 1 (2016): February 2016
Publisher : Universitas Atma Jaya Yogyakarta

Diabetes mellitus (DM) adalah penyakit metabolik yang ditandai hiperglikemik kronis. HbA1c merupakan hasil pemeriksaan untuk melihat kontrol glikemik. Zinc dan Selenium merupakan faktor metaloenzim, berperan dalam mekanisme regulasi dan sintesisÂ insulin.Â Penelitian ini bertujuan untuk mengeksplorasi hubungan antaraÂ Zinc danÂ SeleniumÂ dengan HbA1C pada Â pasien DMT2.Â DesainÂ cross sectionalÂ terhadap pasien DMT2.Â Sampel berjumlah 108 orang pasien di beberapa Rumah sakit di Bandung dari tahun 2011 s/d 2013. Sampel dilakukan secara purposive sampling. Zinc dan Selenium dikumpulkan dengan Semi Quantitative Food Frequency (SQFF).Â HbA1c diukur dengan metode afinitas kromatografi. Data dianalisis denganÂ Fisher ExactÂ danÂ ujiÂ korelasiÂ SpearmanÂ (p<0,05).Â Penelitian ini telah mendapat persetujuan Komite Etik.Â Penelitian menunjukkan bahwa ada hubungan bermakna antara Zinc, Selenium dan HbA1c (p<0,001). Ada hubungan antara Zinc dan Selenium dengan HbA1c,Â sehingga penatalaksanaan diit dengan asupan Zinc dan Selenium sangat diperlukan dalam regulasi pasien DMT2.

Hubungan Kadar HbA1c Dan Rasio TG/HDL Dengan Cystatin-C Serum Pada Pasien Diabetes Melitus Tipe 2 Hadian Widyatmojo; Indranila Kustarini Samsuria; Ria Triwardhani
Medica Hospitalia : Journal of Clinical Medicine Vol. 6 No. 2 (2019): Med Hosp
Publisher : RSUP Dr. Kariadi

Latar belakang: Diabetes melitus (DM) tipe 2 dapat menyebabkan komplikasi salah satunya nefropati. Kontrol glikemik yang dinilai dengan HbA1c dan dislipidemia yang dinilai dengan rasio trigliserida/high density lipoprotein (TG/HDL) diduga berhubungan dengan komplikasi nefropati. Cystatin C merupakan petanda yang dapat menilai kerusakan fungsi ginjal dini. Hubungan kadar HbA1c dan rasio TG/HDL dengan Cystatin C pada pasien DM tipe 2 belum diketahui dengan jelas. Tujuan: Mengetahui hubungan antara kadar HbA1c dan rasio TG/HDL dengan Cystatin C pada pasien diabetes melitus tipe 2. Metode: Penelitian observasional analitik dengan pendekatan belah lintang dilakukan pada bulan April - Juni 2019 melibatkan 34 pasien DM tipe 2 di Puskesmas Karang Ayu yang memenuhi kriteria inklusi dan ekslusi. Pemeriksaan kadar HbA1c menggunakan metode HPLC, rasio TG/HDL dihitung dengan perbandingan TG dengan HDL yang diperiksa menggunakan alat kimia klinik otomatis, kadar Cystatin C menggunakan metode ELISA. Uji statistik menggunakan korelasi Spearman. p < 0,05 dianggap signifikan. Hasil: Rerata±SD kadar HbA1c adalah 8,21±1,65. Median (minimum-maksimum) rasio TG/HDL dan Cystatin C berturut-turut adalah 3,65(1,3–9,7), 0,72(0,46 – 1,22) mg/L. Korelasi HbA1c dengan Cystatin C dan rasio TG/HDL dengan Cystatin C berturut-turut adalah (r = 0,505; p = 0,002) dan (r = 0,471; p = 0,005). Simpulan: Terdapat hubungan positif sedang bermakna antara kadar HbA1c dengan Cystatin C dan rasio TG/HDL dengan Cystatin C pada pasien diabetes melitus tipe 2. Kata kunci: HbA1c, rasio TG/HDL, Cystatin C, DM. The correlation of HbA1c and TG/HDL ratio with serum cystatin C in type 2 diabetes patients Background: Type 2 diabetes mellitus (DM) can cause chronic complications such as nephropathy. Glycemic control assessed with HbA1c and dyslipidemia assessed by the tryglyceride/high density lipoprotein (TG / HDL) ratio is thought to be associated with complications of nephropathy. Cystatin C is a marker that can assess early kidney function damage. Relationship between HbA1c levels and TG / HDL ratio with Cystatin C in type 2 DM patients is not clearly known. Objective: to investigate the correlation of HbA1c and TG/HDL ratio with Cystatin C in acute type 2 diabetes mellitus patients. Methods: Analytic observational study with cross sectional approach was conducted in April-June 2019 involving 34 type 2 diabetes mellitus patients at the Karang Ayu Puskesmas who were screened according to the inclusion and exclusion criteria. The level of HbA1c were measured by the HPLC method, TG/HDL ratio was measured by automatic chemistry analyzer and Cystatin C was measured by ELISA method. Statistical analysis used Spearman Correlation Test. p <0.05 was considered significant. Results: The mean ± SD HbA1c level was 8.21 ± 1.65. The median (minimum-maximum) ratio of TG / HDL and Cystatin C were 3.65 (1.3–9.7), 0.72 (0.46 - 1.22) mg / L, respectively. Correlation of HbA1c with Cystatin C and the ratio of TG / HDL with Cystatin C respectively (r = 0.505; p = 0.002) and (r = 0.471; p = 0.005). Conclusions: There are significant moderate positive correlation between HbA1c with Cystatin C and TG/HDL ratio with Cystatin C in type 2 diabetes mellitus patients. Keywords : HbA1c, TG/HDL ratio, Cystatin C, Type 2 Diabetes mellitus.

Aspek Molekuler Hubungan Asupan Zinc dan Selenium dengan Hemoglobin Glikosilasi pada Pasien Diabetes Mellitus Tipe 2 Indranila Kustarini Samsuria Judiono Yuliati Widiastuti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 1 (2016): February 2016
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v1i1.708

Diabetes mellitus (DM) adalah penyakit metabolik yang ditandai hiperglikemik kronis. HbA1c merupakan hasil pemeriksaan untuk melihat kontrol glikemik. Zinc dan Selenium merupakan faktor metaloenzim, berperan dalam mekanisme regulasi dan sintesis insulin. Penelitian ini bertujuan untuk mengeksplorasi hubungan antara Zinc dan Selenium dengan HbA1C pada pasien DMT2. Desain cross sectional terhadap pasien DMT2. Sampel berjumlah 108 orang pasien di beberapa Rumah sakit di Bandung dari tahun 2011 s/d 2013. Sampel dilakukan secara purposive sampling. Zinc dan Selenium dikumpulkan dengan Semi Quantitative Food Frequency (SQFF). HbA1c diukur dengan metode afinitas kromatografi. Data dianalisis dengan Fisher Exact dan uji korelasi Spearman (p<0,05). Penelitian ini telah mendapat persetujuan Komite Etik. Penelitian menunjukkan bahwa ada hubungan bermakna antara Zinc, Selenium dan HbA1c (p<0,001). Ada hubungan antara Zinc dan Selenium dengan HbA1c, sehingga penatalaksanaan diit dengan asupan Zinc dan Selenium sangat diperlukan dalam regulasi pasien DMT2.

Vitamin D Deficiency Linked To Depression (Systematic Review: Meta Synthesis) Talitha Salsabila; Indranila Kustarini Samsuria; Dwi Retnoningrum; Indah Saraswati
JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL) Vol 10, No 5 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v10i5.30058

Background: Depression is a mood disorder that is marked by a persistent feeling of sadness, loss of interest; reduced energy and vitality; feelings of low self-worth or guilt; loss of appetite; disturbed sleep or insomnia; and slowness of thought or action. Vitamin D deficiency has been associated with depression. When the level of Vitamin D and serotonin is low, that leads to depression.Objective: To analyze whether vitamin D deficiency or insufficiency is associated with depression.Method: This is a Systematic Review Report. Samples were gathered from two journals – PubMed and Science Direct with specific search terms used were 'vitamin D' and 'depression', then were adjusted based on the inclusion criteria and research question. After that, the findings are combined in order to reach a conclusion so that the research question is well answered.Results: There are eleven journals that describe a link between low level of Vitamin D and symptoms of depression. The connection between the two is formed due to the neuroprotector and immunomodulatory effects of Vitamin D.Conclusion: Previous studies have shown that Vitamin D deficiency linked to depression. Keywords : Depression, level of vitamin D, vitamin D serum.

THE DIFFERENCE OF MEAN PLATELET VOLUME AND PLATELET COUNT IN CORONARY HEART DISEASE PATIENTS WITH ACUTE CORONARY SYNDROME AND NON ACUTE CORONARY SYNDROME Aditya Paelo Rizki Sikumbang; Indranila Kustarini Samsuria; Novi Anggriyani; Charles Limantoro
JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL) Vol 10, No 2 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v10i2.29460

Background: Coronary Heart Disease (CHD) is currently one of main causes and the first death in developed and developing countries, including Indonesia. CHD appeared with the clinical appearance of asymptomatic, stable angina pectoris until acute coronary syndrome. ACS is a progressively CHD and takes parameters that describe the process pathogenesis. MPV and platelet count are two parameters in the complete blood count which reflect the activity of platelets in CHD.Objective: This study is to investigate the differences Mean Platelet Volume and Platelet Count in CHD with ACS compared to non ACS.Methods: A cross-sectional study was conducted with collecting data from the medical records of patients with CHD who underwent treatment in the dr. Kariadi General Hospital Medical Center Semarang, the period of January - December 2019. Eighty subjects of 20 non-ACS patients and 60 ACS patients were included in this study which is Mean Platelet Volume and Platelet Count in hematology laboratory were analyzed. The Independent T-test were used for comparing the group means.Results: MPV was found to be higher significantly among ACS patients as compared to non ACS, 9,43 ± 0,3868 fl vs 9,43 ± 0,3868 fl (p-value<0.01) while Platelet Count was lower significantly among ACS patients as compared to non ACS, 282,63 ± 75,084 109/L vs 322,30 ± 41,980 109/L.Conclusion: There were significantly differences in Mean Platelet Volume and Platelet count between patients with ACS and non ACS.

The Difference Levels of Hepcidin and Interleukin-6 between Obese and Non-Obese Type 2 Diabetes Mellitus Mabruratussania Maherdika; Indranila Kustarini Samsuria; Meita Hendrianingtyas; Nyoman Suci Widyastiti; Muji Rahayu
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1780

BACKGROUND: Inflammation occurs in chronic diseases such as type 2 diabetes mellitus (DM). Insulin resistance and inflammation in type 2 DM with obesity can increase interleukin (IL)-6, causing an increase in hepcidin synthesis in the liver. Increased inflammation can exacerbate the course of type 2 DM. This study aims to prove that there are differences in the levels of hepcidin and IL-6 between obese and non-obese type 2 DM.METHODS: This cross-sectional study was conducted on 61 patients with type 2 DM, consist of 22 male and 39 female with an age of more than 40 years. Type 2 DM subjects were obtained from a doctor's diagnosis and were divided into obese and non-obese groups based on body mass index (BMI). Hepcidin and IL-6 levels were examined using the Enzyme-Linked Immunosorbent Assay (ELISA) principle. The data were analyzed using an independent t-test and Mann-Whitney test.RESULTS: The mean level of hepcidin in the obese with type 2 DM group was 25.32±11.54 ng/mL, and non-obese was 11.94±5.31 ng/mL. The median level of IL-6 in the obese with type 2 DM group was 11.9 (5-61) pg/mL, and non-obese 4.8 (1.5-9.8) pg/mL. There was a significant difference in hepcidin and IL-6 levels between the obese and non-obese groups (p=0.000).CONCLUSION: Hepcidin and IL-6 levels in the obese group with type 2 DM were higher than non-obese group.KEYWORDS: type 2 diabetes mellitus, obesity, hepcidin, interleukin-6

Diagnostic Value of Vascular Endothelial Growth Factor C (VEGF-C) and CA 15-3 in Breast Cancer Ajeng Kurnia Wardhani; Indranila Kustarini Samsuria; Meita Hendrianingtyas; Edward Kurnia Setiawan Limijadi; Ria Triwardhani
Journal of Biomedicine and Translational Research Vol 7, No 3 (2021): December 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i3.11681

ABSTRACT Background: expression of VEGF-C and CA 15-3 may be useful to differentiate between malignant and benign breast tumour because VEGF-C plays a role in promoting angiogenesis and lymphangiogenesis in malignant processes and CA 15-3 is the soluble form of transmembrane protein MUC1, a tumour marker which shows higher expression in breast cancer.Objective: to determine the diagnostic value of VEGF-C and CA 15-3 as tumour markers in patients with breast cancer.Methods: this diagnostic study recruited 76 patients that underwent surgical biopsy procedures at Dr. Kariadi and Pantiwilasa Citarum Hospitals Semarang. The VEGF-C and CA 15-3 levels in blood specimens taken before surgical biopsy procedure was determined using ELISA method. An ROC curve and AUC were used to establish the cut-off points and diagnostic value. Pathology examination results from the biopsy specimens were used as the gold standard.Results: the cut-off value for VEGF-C and CA 15-3 were 989.50 pg/mL and 74.00 U/mL. Sensitivity for VEGF-C, CA 15-3 and VEGF-C+CA 15-3 were 76.6%, 64.1% and 89.1%. Specificity for VEGF-C, CA 15-3 and VEGF-C+CA 15-3 were 75.0%, 75.0% and 50.0%. The AUC for VEGF-C, CA 15-3 and VEGF-C+CA 15-3 was 0.831 (95% CI = 0.727-0.934), 0.742 (95% CI = 0.628-0.856) and 0.840 (95% CI = 0.742-0.938).Conclusion: VEGF-C in combination with CA 15-3 is the best diagnostic parameter for breast cancer and has the best accuracy as a tumour marker for breast cancer.

Hubungan Soluble Vascular Cell Adhesion Molecule-1 dengan Asam urat dan Magnesium pada pasien chronic kidney disease Ade Delpita; Indranila Kustarini Samsuria; Herniah Asti Wulanjani
Medica Hospitalia : Journal of Clinical Medicine Vol. 7 No. 1 (2020): Med Hosp
Publisher : RSUP Dr. Kariadi

Latar belakang: Kondisi Chronic kidney disease (CKD) dapat terjadi proses inflamasi dan aterosklerosis akan melibatkan marker inflamasi, disfungsi endotel Soluble vascular cell adhesion molecule-1 (sVCAM-1), asam urat (AU) dan peran elektrolit magnesium berhubungan dengan patofisiologi CKD .sVCAM-1 merupakan biomolekuler marker inflamasi dan disfungsi endotel, AU sebagai marker inflamasi dan magnesium berperan dalam metabolisme elektrolit ginjal. Tujuan: Membuktikan hubungan antara sVCAM-1 dengan asam urat (AU) dan magnesium (Mg) pada pasien CKD. Metoda Penelitian: Penelitian belah lintang dilakukan pada 33 penderita CKD belum pernah hemodialisis, sampel diambil selama bulan Maret - Juni 2019. Nilai Laju Filtrasi Glomerulus (LFG) dihitung menggunakan rumus Cockcroft-Gault. Kadars VCAM-1 diperiksa menggunakan metode Enzyme Link Immunosorbent Assay. Kadar asam urat diperiksa dengan metode fotometrik enzimatik dan magnesium diperiksa dengan kolorimetrik Xylidil blue menggunakan alat Advia. Uji hubungan menggunakan uji Pearson untuk data normal dan Spearman’s untuk data tidak normal. Hasil: Nilai LFG pada penelitan ini 46,48 ± 11,44 ml/min/1,73m2. Median kadarVCAM-1 adalah 715 (564 – 991) ng/dL. Median kadar asam urat adalah 9,2 (7,8 – 15,2) mg/dL dan median untuk kadar magnesium 1,03 (0,79 – 1,5) mmol/dL. Hubungan sVCAM-1 dengan kadar asam urat serum didapatkan korelasi dengan r = 0,488 dan p = 0,004.Terdapat hubungan sVCAM-1 dengan kadar magnesium serum dengan r = -0,442; p = 0,010. Simpulan: Terdapat hubungan positif sedang antara sVCAM-1 dengan asam urat serum, terdapat hubungan negatif sedang antara sVCAM-1dengan magnesium serum. Kata kunci :CKD,LFG, sVCAM-1, asam urat, magnesium Background: Chronic kidney disease (CKD ) is a condition that occurs because of the inflammatory process and atherosclerosis thus involve the marker of inflammation, endhothelial dysfuntion Soluble vascular cell adhesion molecule-1(sVCAM-1), uric acid (UA) and magnesium, associated with the pathophisiology of CKD. VCAM-1 is a marker of inflammation and endhothelial dysfuntion, UA as a marker of inflammation and Mg plays a role in renal electrolytes metabolism. Objective: To prove the relationship between the sVCAM-1 and uric acid (UA) and magnesium (Mg) levels in CKD patients Methode: A cross sectional study conducted on 33 patients with chronic kidney disease who had never hemodialysis during March-June 2019. Value of Glomerular Filtration Rate (GFR) is calculated using the Cockcroft-Gault formula which necessary creatinine levels. Value of GFR is calculated using the Cockcroft-Gault formula.sVCAM-1 levels were examined using the Enzyme Link Immunosorbent Assay method. Uric acid were axamined enzymatic photometric and magnesium levels were examined Xylidil blue colorimetric methods by Advia. Pearson correlation test using the test for normal data and Spearman's when data is not normal. Results: The value of this study GFR at 46.48 ± 11.44 ml/min/1,73m2. Median levels of sVCAM-1 was 715 (564 to 991) ug / dL. Median for uric acid is 9.2 (7.8 to 15.02) mg/dL and magnesium 1,03 (0,79 – 1,5) mmol/dL There is a significant correlation between VCAM-1 with serum uric acid levels showed a correlation with(r = 0.488; p = 0.004). There is a correlation between sVCAM-1 and serum magnesium(r = -0.442; p = 0.010). Conclusion: There is a moderate positive correlation between sVCAM-1 with uric acid serum, there is a moderate negatif correlation between sVCAM-1 and serum magnesium. Keywords: CKD, GFR, sVCAM-1, uric acid, magnesium.

Hubungan Kadar Kreatinin Serum Dengan Kadar Mineral Pada Pasien Chronic Kidney Disease Diah Ayu Kusuma; Indranila Kustarini Samsuria
Medica Hospitalia : Journal of Clinical Medicine Vol. 7 No. 2 (2020): Med Hosp
Publisher : RSUP Dr. Kariadi

Pendahuluan : Chronic Kidney Disease (CKD) merupakan gangguan fungsi ginjal yang irreversible, yaitu kemampuan tubuh gagal untuk mempertahankan metabolisme dan keseimbangan cairan dan elektrolit dan juga mineral. Sebagian besar proses metabolisme memerlukan dan dipengaruhi oleh elektrolit. Konsentrasi elektrolit mineral yang tidak normal dapat menyebabkan banyak gangguan . Saat fungsi ginjal semakin memburuk menjadi Stadium CKD 4 dan 5, ekskresinya cenderung berkurang dan tidak dapat dikompensasi. Tujuan penelitian ini adalah melihat pengaruh CKD pada mineral yaitu magnesium dan calcium. Metode : Penelitian ini adalah penelitian observasional retrospektif dengan pendekatan belah lintang (cross sectional) dengan melihat rekam medik pada senter tunggal di RSUP Dr. Kariadi Semarang selama periode 1 Januari 2017 – 31 Desember 2018 untuk kemudian dilihat hubungan pengaruh kadar kreatinin serum dengan magnesium dan calcium pada pasien CKD dengan terapi hemodialisis. Hasil dan pembahasan: Terdapat hubungan signifikan positif lemah antara kreatinin serum dengan magnesium (p=0,032, r= 0,327). Tidak terdapat hubungan antara kreatinin serum dengan kadar kalsium. Simpulan : Dari hasil penelitian dapat disimpulkan bahwa secara statistik terdapat hubungan yang bermakna antara kadar kreatinin serum dengan magnesium pada pasien Chronic Kidney Disease (CKD) dengan terapi hemodialisis. Kata Kunci Kreatinin, mineral, magnesium Chronic Kidney Disease, hemodialisis. ABSTRACT Introduction: Chronic Kidney Disease (CKD) is an irreversible kidney function disorder, which is the body's ability to fail to maintain metabolism and balance fluid and electrolytes and minerals. Most metabolic processes require and are influenced by electrolytes. Abnormal mineral electrolyte concentrations can cause many disturbances. When kidney function getting worse to stages CKD 4 and 5, the excretion tends to decrease and cannot be compensated. The purpose of this study is to look at the effect of CKD on minerals namely magnesium and calcium. Method: This study was a retrospective observational study with a cross sectional approach by looking at medical records at RSUP Dr. Kariadi Semarang during the period of 1 January 2017 - 31 December 2018 to see the correlation of influence of serum creatinine levels with magnesium and calcium in CKD patients with hemodialysis therapy. Results and discussion: There was a significantly weak positive correlation between serum creatinine and magnesium (p = 0.032, r = 0.327). There is no correlation between serum creatinine and calcium levels. Conclusion: The results of the study it can be concluded that there is a statistically significant correlation between serum creatinine levels with magnesium in Chronic Kidney Disease (CKD) patients with hemodialysis therapy. Keywords Creatinine, minerals, magnesium Chronic Kidney Disease, hemodialysis.

HUBUNGAN DIPSTIK URIN DAN FLOWSITOMETRI URIN DENGAN KULTUR URIN PADA INFEKSI SALURAN KEMIH (ISK) Bilma Riasari Guspa; Muji Rahayu; Indranila Kustarini Samsuria
Media Medika Muda Vol 3, No 1 (2018)
Publisher : Faculty of Medicine Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.596 KB)

Latar belakang: Infeksi saluran kemih (ISK) adalah adanya mikroorganisme dalam urin. Bakteriuria bermakna bila pertumbuhan mikroorganisme murni lebih dari 105 colony forming units (CFU) pada kultur urin. Pemeriksaan laboratorium untuk deteksi ISK adalah dipstik urin (nitrit dan lekosit esterase) dan flowsitometri (jumlah leukosit dan bakteri). Tujuan dari penelitian ini adalah untuk menganalisis hubungan antara hasil pemeriksaan dipstik dan flowsitometri dengan pemeriksaan kultur urin.Metode: Penelitian belah lintang pada 42 sampel pria yang menderita ISK di RSUP Dr. Kariadi Semarang selama bulanAgustus–September 2017. Metode pemeriksaan disptik Combur adalah kolorimetri, metode flowsitometri menggunakan Sysmex UF1000i, dan kultur menggunakan metode konvensional. Uji data korelasi menggunakan contingency coefficient test.Hasil: Tidak terdapat hubungan bermakna dari dipstrik nitrit terhadap kultur (r=0,190 dan p=0,210), terdapat hubungan positif lemah antara dipstik lekosit esterase dengan kultur (r=0,363 dan p=0,012), terdapat hubungan positif kuat dari flowsitometri lekosit terhadap kultur (r=0,534 dan p=0,000) dan terdapat hubungan positif kuat antara flowsitometri bakteri dengan kultur (r=0,534, p=0,000). Simpulan: Tidak terdapat hubungan bermakna dari dipstik nitrit terhadap kultur. Terdapat hubungan bermakna dari dipstrik lekosit esterase, flowsitometri lekosit, terdapat hubungan yang bermakna antara flowsitometri bakteri dengan kultur. Penelitian lebih lanjut diperlukan untuk menganalisis faktor-faktor yang berpengaruh pada diagnostik ISK.

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