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All Journal Jurnal Bahan Alam Terbarukan FLUIDA Fullerene Journal of Chemistry International Journal of Applied Technology Research
Edi Wahyu Sri Mulyono
Jurusan Teknik Kimia, Politeknik Negeri Bandung
Aerospace Engineering Automotive Engineering Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Physics

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THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE Djenar, Nancy Siti; Mulyono, Edi Wahyu Sri
Jurnal Bahan Alam Terbarukan Vol 6, No 1 (2017): June 2017 [Nationally Accredited]
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v6i1.7950

Abstract

X. campestris is aerobic bacteria producing extracellular biopolymers (EPS, extracellular polysaccharide) known as xanthan gum. To determine the technology and the process conditions appropriate to the formation of this product, therefore the understanding of both the kinetics of growth and metabolite production of bacteria is needed. In this research, to assess the growth of X. campestris using the method of calculation of dry cell weight. For determining the kinetics of production of metabolite used substrates of 4% liquid sugar  from cassava starch hydrolysate. From this research was showed that X. campestris maximum growth in NB medium obtained in about 58thhour, at the growth rate of about 0.04 g / hour, stationary phase obtained at the 60th hour with a maximum dry cell weight of 2.7688 g/L and specific growth rate (μ) of X. campestris amounted to 0.043 hour-1. Based on the kinetic curves both on growth and its metabolite production, X.campestris has non-growth associated product pattern. In this case the production of  xanthan gum  occured after cell growth stopped then  its product is a secondary metabolite with highest amount  of 3.73 g / L at 102nd hour, ie the 4th day of fermentation. Overall of this research indicated that  Nutrient Broth (NB) may be used for the growth of X. campestris. But based on the value of μ above, the rate of cell reproduction was still low. Liquid sugar can be used as a substrate to produce xanthan gum. However to increase its productivity, there should be an addition of other carbon or energy and nitrogen sources.
THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE Djenar, Nancy Siti; Mulyono, Edi Wahyu Sri
Jurnal Bahan Alam Terbarukan Vol 6, No 1 (2017): June 2017 [Nationally Accredited]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v6i1.7950

Abstract

X. campestris is aerobic bacteria producing extracellular biopolymers (EPS, extracellular polysaccharide) known as xanthan gum. To determine the technology and the process conditions appropriate to the formation of this product, therefore the understanding of both the kinetics of growth and metabolite production of bacteria is needed. In this research, to assess the growth of X. campestris using the method of calculation of dry cell weight. For determining the kinetics of production of metabolite used substrates of 4% liquid sugar  from cassava starch hydrolysate. From this research was showed that X. campestris maximum growth in NB medium obtained in about 58thhour, at the growth rate of about 0.04 g / hour, stationary phase obtained at the 60th hour with a maximum dry cell weight of 2.7688 g/L and specific growth rate (μ) of X. campestris amounted to 0.043 hour-1. Based on the kinetic curves both on growth and its metabolite production, X.campestris has non-growth associated product pattern. In this case the production of  xanthan gum  occured after cell growth stopped then  its product is a secondary metabolite with highest amount  of 3.73 g / L at 102nd hour, ie the 4th day of fermentation. Overall of this research indicated that  Nutrient Broth (NB) may be used for the growth of X. campestris. But based on the value of μ above, the rate of cell reproduction was still low. Liquid sugar can be used as a substrate to produce xanthan gum. However to increase its productivity, there should be an addition of other carbon or energy and nitrogen sources.
Simulation Data Acquisition and Control for LNG Custody Transfer Based on ISO 8943 Muhammad Arman; Edi Wahyu Sri Mulyono; Muhamad Anda Falahuddin; Wirenda Sekar Ayu
Jurnal Internasional Penelitian Teknologi Terapan Vol 2 No 1 (2021): April 2021
Publisher : Bandung State Polytechnic (Politeknik Negeri Bandung)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/ijatr.v2i1.45

Abstract

Liquefied Natural Gas (LNG) is a complex mixture of low-molecular-weight hydrocarbons with nitrogen as a principal inert impurity. Nowadays, the instrumentation control tools are needed in LNG Commercialization to ensure the LNG is safely transported, while minimizing the energy losses across the network. This research was a preliminary design that used LNG custody transfer control tools referred the ISO 8943:2007 standard. The temperature was measured by thermocouple (Rosemount transmitter 3144 series), while the pressure was measured using pressure transmitter (Rosemount transmitter 3051 series). The simulated signal temperature was done by temperature control box, while the simulated pressure was represented by WIKA pressure calibrator. Data from transmitter was sent to the LabVolt 9063 series that has data acquisition for Software Development Kit (SDK) enabled. Previously, the LabVolt 9063 did not recognized the signal from the transmitter. The SDK software bridged the LabVolt hardware with the LabView software. In the simulated system, temperature alarm will be triggered when the temperature value exceeds 20oC. For simulated pressure, the alarm will respond if pressure value exceeds 30 psi. From the results of this research, the communication between hardware and software worked properly. The data acquisition system was stable during the data collection stage, processing and displaying. In addition, the alarm indicator for both temperature and pressure was met within the threshold value.
Studi Literatur Potensi Ekstrak Zat Warna Alam sebagai Indikator Asam Basa Alternatif Alifa Gunawan Yusuf; Naila Najiyah; Edi Wahyu Sri Mulyono; Fauzi Abdilah
Jurnal Kimia Fullerene Vol 6 No 2 (2021): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v6i2.338

Abstract

Currently, the use of acid-base indicators is limited to synthetic indicators, which are relatively expensive and not environmentally friendly. Therefore, an alternative acid-base indicator is needed from natural ingredients containing anthocyanins which are relatively cheaper, easy to obtain, and environmentally friendly so that they can replace the function of synthetic indicators. For this reason, it is necessary to research beforehand on the sources of raw materials that can be used as alternative acid-base indicators, how the method and what solvents are suitable, as well as the character of the anthocyanin dye extract obtained. In this research, a literature study related to the potential of natural dye extracts was carried out as an alternative acid-base indicator. From the results of the literature study, it can be seen that natural ingredients derived from groups of vegetables, flowers, fruits, and tubers have the potential as raw materials for making alternative acid-base indicators. The best method for extracting anthocyanin dyes from natural materials is the maceration method and the solvent that is suitable for use is ethanol-HCl solvent. The results of titration using natural dye extract indicators have similarities with the results of titrations using phenolphthalein and methyl orange indicators so that natural dye extract indicators can be used as alternative indicators in acid-base titrations.
Penapisan Zat Warna Alam Golongan Anthocyanin dari Tanaman Sekitar Sebagai Indikator Asam Basa Budi Santoso; Edi Wahyu Sri Mulyono
Fluida Vol 11 No 2 (2015): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v11i2.80

Abstract

Ekstraksi zat warna alami dari beberapa macam bunga, antara lain bunga Tapak dara (Catharanthus roseus L.), Mawar merah (Rosa L.), Alamanda kuning (Allamanda canthartica), bunga rambat ungu (Ipomoea cairica), dan bunga saputangan (Maniltoa grandiflora) memiliki potensi untukdigunakan sebagai indikator asam basa. Demikian pula dengan ekstrak zat warna alami dari biji beras hitam (Oryza sativa L.) daun bayam merah (Celosia argentea), kubis ungu/merah (Brassica oleracea var.) dan umbi bit merah (Beta vulgaris). Hampir keseluruhan bagian tanaman tersebutmengandung zat warna alam yang dapat digunakan sebagai indikator alami, yakni memiliki perubahan warna yang jelas pada pH tertentu. Zat warna alam ini pada umumnya tersusun oleh senyawa antosianin. Ekstrak kubis ungu diduga mengandung kadar antosianin yang paling tinggidan menunjukkan perubahan warna yang paling lengkap pada semua perubahan nilai pH. Persen berat hasil ekstraksi senyawa golongan Antosianin dari kubis ungu dengan menggunakan pelarutair adalah 0,22 % b/b sedangkan dengan HCl 0,1 N dalam metanol 0,36 % b/b. Konsentrasi total anthocyanidin dihitung berdasarkan Cyanidin 3-glucosida dengan absorptivitas molar 26,900 L mol-1 cm-1.
Co-Authors Alifa Gunawan Yusuf Budi Santoso Fauzi Abdilah Muhamad Anda Falahuddin Naila Najiyah Nancy Siti Djenar Wirenda Sekar Ayu