Garuda - Garba Rujukan Digital

THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE Djenar, Nancy Siti; Mulyono, Edi Wahyu Sri
Jurnal Bahan Alam Terbarukan Vol 6, No 1 (2017): June 2017 [Nationally Accredited]
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v6i1.7950

X. campestris is aerobic bacteria producing extracellular biopolymers (EPS, extracellular polysaccharide) known as xanthan gum. To determine the technology and the process conditions appropriate to the formation of this product, therefore the understanding of both the kinetics of growth and metabolite production of bacteria is needed. In this research, to assess the growth of X. campestris using the method of calculation of dry cell weight. For determining the kinetics of production of metabolite used substrates of 4% liquid sugarÂ from cassava starch hydrolysate. From this research was showed that X. campestris maximum growth in NB medium obtained in about 58thhour, at the growth rate of about 0.04 g / hour, stationary phase obtained at the 60th hour with a maximum dry cell weight of 2.7688 g/L and specific growth rate (Î¼) of X. campestris amounted to 0.043 hour-1. Based on the kinetic curves both on growth and its metabolite production, X.campestris has non-growth associated product pattern. In this case the production of Â xanthan gumÂ occured after cell growth stopped thenÂ its product is a secondary metabolite with highest amount Â of 3.73 g / L at 102nd hour, ie the 4th day of fermentation. Overall of this research indicated thatÂ Nutrient Broth (NB) may be used for the growth of X. campestris. But based on the value of Î¼ above, the rate of cell reproduction was still low. Liquid sugar can be used as a substrate to produce xanthan gum. However to increase its productivity, there should be an addition of other carbon or energy and nitrogen sources.

THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE Djenar, Nancy Siti; Mulyono, Edi Wahyu Sri
Jurnal Bahan Alam Terbarukan Vol 6, No 1 (2017): June 2017 [Nationally Accredited]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v6i1.7950

X. campestris is aerobic bacteria producing extracellular biopolymers (EPS, extracellular polysaccharide) known as xanthan gum. To determine the technology and the process conditions appropriate to the formation of this product, therefore the understanding of both the kinetics of growth and metabolite production of bacteria is needed. In this research, to assess the growth of X. campestris using the method of calculation of dry cell weight. For determining the kinetics of production of metabolite used substrates of 4% liquid sugarÂ from cassava starch hydrolysate. From this research was showed that X. campestris maximum growth in NB medium obtained in about 58thhour, at the growth rate of about 0.04 g / hour, stationary phase obtained at the 60th hour with a maximum dry cell weight of 2.7688 g/L and specific growth rate (Î¼) of X. campestris amounted to 0.043 hour-1. Based on the kinetic curves both on growth and its metabolite production, X.campestris has non-growth associated product pattern. In this case the production of Â xanthan gumÂ occured after cell growth stopped thenÂ its product is a secondary metabolite with highest amount Â of 3.73 g / L at 102nd hour, ie the 4th day of fermentation. Overall of this research indicated thatÂ Nutrient Broth (NB) may be used for the growth of X. campestris. But based on the value of Î¼ above, the rate of cell reproduction was still low. Liquid sugar can be used as a substrate to produce xanthan gum. However to increase its productivity, there should be an addition of other carbon or energy and nitrogen sources.

Isolasi dan Pemurnian Protein dari Lembaga Jagung (Corn Germ) Menggunakan Metode Presipitasi dan Dialisis: Protein Isolation and Purification from Corn Germ Using Precipitation and Dialysis Methods Nancy Siti Djenar; Joko Suryadi
KOVALEN: Jurnal Riset Kimia Vol. 8 No. 1 (2022): April Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2022.v8.i1.15790

The protein contained in corn germ can be used as a raw material that has the potential to provide many benefits in the food, pharmaceutical, and plastic industries. Corn germ with the highest content of protein is around 12-18.4%. In this study, protein from corn germ was isolated and homogenized using tris buffer HCl at pH 7.2. To obtain the protein fraction, precipitation was carried out (salting-out) using a solution of (NH4)2SO4 with saturation levels of 25%, 50%, and 70% respectively. The purification process was carried out by dialysis for 24 hours. The dialysis produced pure protein with a concentration of 4.161%. Based on the identification of functional groups using an FT-IR spectrophotometer, it was shown that the isolated and purified corn germ protein contained four absorption bands, including amide A (3211.48 cm-1 and 3226.91 cm-1), amide I (1633 cm-1 and 1629.85 cm-1), amide II (1527 cm-1 and 1552.70 cm-1) and amide III (1296.16 cm-1). The four absorption bands correspond to the wavenumbers of corn protein stated in the literature and are estimated to contain albumin, globulin, glutelin, and zein.

Pengaruh Metode Hidrolisis Terhadap Karakteristik Kimia Senyawa Kalsium Hasil Ekstraksi dari Cangkang Telur Ayam: The Effect of Hydrolysis Methods on Chemical Characteristics of Extracted Calcium Compounds from Chicken Eggshells Yurikke Julieta Permata Putri Sunarya; Yuni Kustiar; Nancy Siti Djenar; Ayu Ratna Permanasari
KOVALEN: Jurnal Riset Kimia Vol. 8 No. 1 (2022): April Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2022.v8.i1.15803

Preparation of calcium compounds such as calcium hydroxide, calcium carbonate, and calcium oxide from chicken eggshells can be done through conventional methods that are cheaper and more practical, such as the sol-gel hydrolysis method and the coprecipitation method. In this study, the synthesis of calcium compounds from domestic chicken eggshells was carried out using two methods: the coprecipitation method and sol-gel method. Coprecipitation method consists of acid hydrolysis and alkaline hydrolysis. Sol-gel method consists of modification I and modification II. The AAS results showed that the calcium content from the hydrolysis of acids, alkaline, sol-gel modification I, and sol-gel modification II: 14.50%, 6.64%, 6.68%, and 9.93%, respectively. The FTIR showed that calcium compounds have four characteristic absorption bands, including O–H (3641.60 and 3448.72 cm-1) derived from Ca(OH)2 and H2O products, C=O (2981.95 - 1799 cm-1), and C–O (1448.54 – 874.68 cm-1) both of which from carbonate ions (CO3-) in CaCO3 both from eggshell and from the resulting calcium compounds, and Ca-O (711.73 cm-1) derived from CaO products. The results showed that the modification ll sol-gel method was the best because calcium compounds consisting of CaCO3, Ca(OH)2, and CaO were produced in a smooth texture, white color, and with the highest calcium content compared to other methods.

Penyisihan Kadar Amoniak dalam Limbah Cair Industri Pupuk Menggunakan Sequencing Batch Reactor Robby Sudarman; Herawati Budiastuti; Nancy Siti Djenar; Efniarsi S. Panggalo; Ajie Nurhasyim
Fluida Vol 13 No 2 (2020): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v13i2.2264

ABSTRAK Sifat toksik nitrogen amoniak dalam limbah cair berbahaya bagi kehidupan sehingga perlu untuk dihilangkan. Pada penelitian ini penyisihan kadar amoniak yang tinggi dalam limbah cair industri pupuk dilakukan melalui proses nitrifikasi dan denitrifikasi menggunakan Sequencing Batch Reactor (SBR). Dua reaktor SBR digunakan dimana pada reaktor pertama (R1) dilakukan pengadukan dan pada reaktor kedua (R2) dilakukan aerasi. Hasil penelitian menunjukkan bahwa pada pembebanan 0,787 mg amoniak/L/hari dan Hydraulic Retention Time (HRT) 20 hari, efisiensi penyisihan kadar amoniak mampu dicapai pada kisaran 71,26%-90,91% (R1) dan 62,32% -92,21% (R2). Pengukuran pH pada HRT 20 hari berada pada rentang 8,25-8,65 pada R1 dan pH 8,34-8,73 pada R2. Pada HRT 15 hari, efisiensi penyisihan kadar amoniak efluen pada R1 menurun yaitu dari 90,89 % menjadi 86,81% dan pada R2 menurun dari 90,59% menjadi 40,99 %. Pada HRT 15 hari, efisiensi penyisihan kadar amoniak pada R1 lebih baik dibandingkan pada R2 yang disebabkan adanya penambahan aerasi pada R1 di akhir penelitian. Kata kunci: amoniak, limbah cair, industri pupuk, sequencing batch reactor ABSTRACT The toxic nature of ammonia nitrogen in liquid waste is dangerous for life, so it needs to be eliminated. In this study, the removal of high ammonia levels in the fertilizer industry wastewater was carried out through a process of nitrification and denitrification using the Sequencing Batch Reactor (SBR). Two SBR reactors were used where the first reactor (R1) was stirred and the second reactor (R2) was aerated. The results showed that at a load of 0.787 mg of ammonia/L/day and 20 days of Hydraulic Retention Time (HRT), the efficiencies of removal of ammonia levels were achieved in the range of 71.26%-90.91% (R1) and 62.32%- 92.21% (R2). The pH measurements at 20 days of HRT were in the range of 8.25-8.65 at R1 and pH 8.34-8.73 at R2. At 15 days of HRT, the effluent ammonia level removal efficiency at R1 decreased from 90.89% to 86.81% and at R2 decreased from 90.59% to 40.99%. At 15 days of HRT, the efficiency of ammonia level removal at R1 was better than that in R2 due to the addition of aeration to R1 at the end of the study. Keywords: ammonia, liquid waste, fertilizer industry, sequencing batch reactor

UTILIZATION OF ANTHOCYANIN IN EDIBLE FILM AS COCONUT MILK FRESHNESS INDICATOR: English Utary Nur Rachmani Fauzan; Astrinida Rizkina Dwi Pancani; Fitria Yulistiani; Nancy Siti Djenar
Jurnal Kimia Riset Vol. 7 No. 1 (2022): June
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v7i1.35706

Technology in smart packaging continues to evolve, one of which is packages equipped with food freshness indicators to monitor food safety and quality. These indicators can be formed by immobilizing anthocyanin into edible film and then changing its color to pH changes. The research aims to determine the immobilization time, test the color stability of the edible film indicator to temperature changes, determine the characterizations of the edible film indicator based on the %relative standard deviation (%RSD) value, and apply the edible film indicators as coconut milk freshness indicators. The best immobilization time was in the 90th minute with %RSD=0%. The edible film indicators did not change color from 10 to 50°C with %RSD=0%. The characterizations of edible film indicators on pH 6, 7, 8, and 10 had response times of 6.68 minutes; 9.49 minutes; 3.57 minutes; and 4.37 minutes; periods of 14 days, 13 days, 14 days, and 16 days; and good reproducibility at pH 6 and 10 and not good at pH 7 and 8. Overall, based on anthocyanin color changes, an edible film indicator can be used as a freshness indicator in coconut milk but cannot indicate the pH value of coconut milk.

Pembuatan dan Pengujian Pupuk Organik Cair dari Limbah Kulit Buah-buahan dengan Penambahan Bioaktivator EM4 dan Variasi Waktu Fermentasi Dewi Widyabudiningsih; Lina Troskialina; Siti Fauziah; Shalihatunnisa Shalihatunnisa; Riniati Riniati; Nancy Siti Djenar; Mentik Hulupi; Lili Indrawati; Ahmad Fauzan; Fauzi Abdilah
Indonesian Journal of Chemical Analysis (IJCA) Vol. 4 No. 1 (2021): Indonesian Journal of Chemical Analysis
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijca.vol4.iss1.art4

Produksi olahan pangan dari buah-buahan selalu menghasilkan limbah kulit dalam pengolahannya. Limbah tersebut hanya dibuang dan dibiarkan menumpuk begitu saja oleh mansyarakat. Apabila tidak ditangani secara cepat akan menghasilkan bau yang tidak sedap sehingga akan mencemari lingkungan. Salah satu alternatif yang dapat dilakukan yaitu dengan cara mengolah limbah tersebut menjadi pupuk organik cair dengan proses fermentasi. Penelitian ini dilakukan untuk mengetahui kandungan hara makro dan kualitas dari pupuk organik cair, mengetahui waktu fermentasi optimum, harga pokok produksi dan BEP dari proses pembuatan pupuk organik cair. Penelitian ini dilakukan dengan cara menambahkan EM4, ragi, dan air gula ke dalam reaktor yang berisi limbah kulit buah yaitu kulit pisang, mangga dan nanas. Proses fermentasi dilakukan selama 34 hari dan dilakukan pengambilan sampel pada hari ke-7, 14, 24 dan 34 untuk dianalisis kandungan hara makro yang terdiri dari C-Organik, P, dan K dengan metode spektrofotometri UV/VIS, SSA dan N dengan metode Kjeldahl. Pada penelitian ini dihasilkan pupuk organik cair yang terbaik yaitu campuran limbah kulit pisang, mangga dan nanas dengan waktu fermentasi 7-14 hari dan kandungan unsur C-Organik, N-total, K2O, dan P2O5 masing-masing sebesar 17,4; 6,05; 2,50 dan 0,15 %. Pupuk organik cair yang dihasilkan sudah memenuhi baku mutu dari Permentan Nomor 261 tahun 2019 kecuali kandungan P2O5, walaupun demikian pupuk organik cair yang diperoleh ini memiliki kualitas yang lebih baik dibanding beberapa pupuk yang sudah dijual secara komersial. Biaya pokok produksi dari pembuatan POC ini sebesar Rp 770.554 dengan Break Event Point (BEP) pada 10 liter.

Penentuan Jenis Pelarut Ekstraksi Terbaik dan Pengaruh Waktu Fermentasi pada Aktivitas β-galaktosidase dari Lactobacillus lactis Geraldy Andreas Sitepu; Army Adi Sutanningsih; Nancy Siti Djenar
Prosiding Industrial Research Workshop and National Seminar Vol 11 No 1 (2020): Prosiding 11th Industrial Research Workshop and National Seminar (IRWNS)
Publisher : Politeknik Negeri Bandung

Enzim β-galaktosidase (laktase) digunakan dalam produksi susu rendah laktosa untuk konsumsi penderita intoleransi laktosa. Dalam proses metabolismenya, enzim ini dapat menghidrolisis laktosa menjadi glukosa dan galaktosa. Lactobacillus lactis merupakan salah satu bakteri asam laktat yang dapat menghasilkan β_galaktosidase secara intraseluler. Dalam penelitian ini β_galaktosidase dari Lactobacillus lactis diekstraksi melalui tiga jenis pelarut, yaitu isoamyl Alkohol, SDS-Kloroform, dan Toluen-Aseton. Substrat yang digunakan adalah oNPG (o-nitrophenol-β-Dgalactopyranoside), dimana β-galaktosidase akan menghidrolisis substrat menjadi oNP dan β-D-galactose. Berdasarkan nilai oNP yang tertinggi sebesar 8,206 µmol, pada pH 7 dengan waktu dan suhu inkubasi masing masing 24 jam dan 37oC, maka pelarut Toluen-Aseton lebih baik dari kedua pelarut lainnya. Sedangkan nilai aktivitas β-galaktosidase tertinggi yaitu sebesar 13,02 U/mL dengan waktu inkubasi dan fermentasi masing-masing 15 menit dan 24 jam.

Pemanfaatan Herbal Bunga Telang Sebagai Peluang Usaha Bagi Kelompok Wanita Tani (KWT) Di Desa Arjasari Kabupaten Bandung Riniati Riniati; Nancy Siti Djenar; Lili Indrawati; Dewi Widyabudiningsih; Lina Troskialina; Mentik Hulupi; Yusuf Sofyan; Sudrajat Harris Abdulloh
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 7 No 2 (2022): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v7i2.2873

Bunga telang (Clitoria ternatea) merupakan tanaman yang mudah dibudidayakan. Bunganya yang unik berwarna biru dapat dijadikan zat warna alami untuk berbagai jenis makanan dan minuman. Kandungan antoksidan yang tinggi dalam bunga telang menjadikan bunga ini menjadi trendi sebagai minuman herbal untuk meningkatkan imun tubuh di masa pandemi ini. Sehubungan dengan hal ini maka bunga telang dapat menjadi peluang usaha untuk petani khususnya Kelompok Wanita Tani Rengganis yang ada di Desa Arjasari Kabupaten Bandung. Untuk meningkatkan usaha pembuatan serta meningkatkan mutu tanaman herbal khususnya bunga telang, Politeknik Negeri Bandung (Polban) melalui program Pengabdian Kepada Masyarakat (PkM) memberikan penyuluhan yang mencakup pengenalan herbal dan manfaatnya, penanaman secara organik menggunakan pupuk organik cair, teknik pengeringan dan pengolahan bunga telang yang dapat menghasilkan berbagai produk berbahan dasar bunga telang, serta teknik pemasaran untuk memanfaatkan peluang bisnis dari bunga telang. Kegiatan ini mendapat respon dan antusiasme yang tinggi dari masyarakat setempat, karena secara umum peluang usaha ini sesuatu yang baru untuk mereka. Berdasarkan kuisioner yang dibagikan kepada peserta kegiatan ini diharapkan dapat berlanjut. Sehingga dapat meningkatkan kesehatan, kesejahteraan serta perekonomian masyarakat Desa Arjasari dan sekitarnya.

Pengaruh Penambahan Minyak Zaitun Terhadap Karakteristik dan Reologi Edible Film Berbahan Dasar Gluten: The Effect of Addition of Olive Oil on The Properties and Rheology of Gluten-Based Edible Films Nancy Siti Djenar; Joko Suryadi; Nisa Siti Nursaadah; Erina Putri
KOVALEN: Jurnal Riset Kimia Vol. 8 No. 2 (2022): August Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2022.v8.i2.15948

Gluten vegetable protein can be used as an alternative source of biopolymer as the basic material for edible film other than polysaccharides. Gluten-based edible films have a poor water vapor barrier and high cohesive and viscoelastic properties, therefore the addition of lipids and plasticizers is required. In this study, olive oil was added to reduce its permeability to water vapor, while glycerin was added to produce a more flexible edible film. The results showed that the addition of 1% olive oil could reduce the lowest water vapor transmission rate of 9.14 g/m2/24 hours with a thickness of 0.248 mm, tensile strength of 16.64 mPa, and elongation of 419.5%. The four characteristics are in accordance with the Japanese Industrial Standard. The antimicrobial testing on edible films showed that the addition of 0-2% olive oil could inhibit the growth of E. coli, while A. niger and R. oryzae 0-2% olive oil could not inhibit the growth of the two fungi. The measurement of optical properties showed that the transparency of the edible film was highest at the addition of 0% olive oil at 55%. The highest opacity value was with the addition of 1% olive oil, which is 2.96. The Fourier Transform Infrared (FTIR) identification showed that the edible film added with 1% olive oil had three characteristic absorption bands from gluten, olive oil, and an absorption band from glycerin. These bands indicate that olive oil, glycerin, and gluten do not react but only physically interact. The measurement using Scanning Electron Microscopy (SEM) and Energy Dispersive Spectrometer (EDS) showed that the microstructure of gluten-based edible film produces a varied structure where the gluten structure network can be clearly observed and contains elements of C, O, N where the intensity of C and O elements of 160-400 cps and 30-100 cps, respectively.

Title

Found 12 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 12 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 12 Documents
Search