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All Journal JURNAL ILMIAH PLATAX
Dhebby Purba
("Faculty of Fisheries and Marine Science Sam Ratulangi University")

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Seagrass Community Structure at Ratatotok Beach, Southeast Minahasa Regency Dhebby Purba; Billy Wagey; Alfret Luasunaung; Indri Manembu; Deiske Sumilat; Rose Mantiri
Jurnal Ilmiah PLATAX Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.47824

Abstract

The purpose of this research is to know about the type of seagrass, the community structure of seagrass based on the density of type, the density of the relatively, type of frequency, frequency relatively, type of cover, cover relatively, diversity index, dominance and parameter index in surrounding water environment of seagrass at Ratatotok beach. The result of this research is identifying 4 species, namely; Thalassia hemprichii, Enhalus acoroides, Syringodium isoetifolium dan Halodule pinifolia. The value of density is 39 indv/m2, the value type of frequency is 7.9, the value type of cover is 39.15, importance value index had 159.04%. Thalassia hemprichii, diversity index is “medium” with an average value (H’) of 2.63 and a higher importance index with an average value of C of 0.82. Surrounding waters at Ratototok Beach got a “medium” value of seagrass cover value is 39%. Environmental parameters in surrounding waters at Ratatotok beach had a value within; Temperature 31.95 0C, salinity 29.79o/oo, pH 7.62, DO 5.60 mg/L and the type of substrate found is sandy, sandy mud and coral fragments. Keywords: Seagrass, Community structure, Ratatotok beach. Abstrak Tujuan dilakukan penelitian ini yaitu mengetahui jenis lamun, struktur komuntas lamun berdasarkan kepadatan jenis, kepadatan relatif, frekuensi jenis, frekuensi relatif, penutupan jenis, penutupan relatif, indeks keanekaragaman, indeks dominansi dan parameter lingkungan perairan padang lamun yang berada di sekitar Pantai Ratatotok. Hasil yang di peroleh dari penelitian ini teridentifikasi 4 spesies, yaitu: Thalassia hemprichii, Enhalus acoroides, Syringodium isoetifolium dan Halodule pinifolia. Dengan nilai kepadatan 39 ind/m2, frekuensi jenis 7,9, penutupan jenis 39,15, Indeks nilai penting (INP) diperoleh nilai 159,04 %,  pada Thalassia hemprichii, Indeks keanekaragaman “sedang” dengan nilai rata-rata (H’) 2,63 dan indeks dominansi tinggi dengan nilai rata-rata C 0,82. Berdasarkan kategori penutupan lamun yang terdapat di sekitar Perairan Ratatotok dikategorikan “sedang” dengan nilai penutupan 39%. Parameter lingkungan antara lain: suhu 31,95 0C, salinitas 29,79 o/oo, pH 7,62, DO 5,60 mg/l dan jenis substrat yang di temukan adalah berpasir, pasir berlumpur dan pecahan karang. Kata kunci : Lamun, Struktur Komunitas, Pantai Ratatotok
Analysis Of Bacteria Community In The Plastic Waste Dhebby Purba; Veibe Warouw; Rizald Rompas; Deiske Sumilat; Reni Kreckhoff; Elvy Ginting
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.29877

Abstract

Plastic waste is a general problem for the environment. Therefore, more ways of plastic waste management are required. One of the plastic waste management process is microbiological biodegradation using bacteria. This research aims to identify the bacterial community in Polyethylene Terephthalate type plastic waste by molecular analysis. In order to identify the community of bacteria, it is necessary to isolate the DNA of uncultured bacteria. In this research, the isolation of the DNA of uncultured bacteria from plastic waste was carried out by the Qiagen DNeasy® PowerSoil Pro Kit handbook. The DNA genome was subsequently examined using electrophoresis gel and UV-Vis spectrophotometer. The DNA genome was then amplified using Polymerase Chain Reaction and the bacteria were identified using Next Generation Sequencing (NGS) analysis. On the results of this study, the uncultured bacteria DNA from plastic waste was isolated with purification value of 1. The amplified DNA genome was detected by DNA bands through gel electrophoresis gel at ~1500 bp. The bacteria in the plastic waste were Proteobacteria, Cyanobacteria, Actinobacteria, Chlorobi, Bacteroidetes, Firmicutes, Chloroflexi, Thaumarchaeuta and Gemmattimonadetes with the highest relative abundance of Proteobacteria.Keywords : Bacteria, Deoxyribo Nucleic Acid ( DNA), Plastic Waste, UnculturedAbstrakSampah plastik merupakan masalah bagi lingkungan oleh sebab itu diperlukan pengelolaan yang baik. Salah satu pengelolaan sampah plastik yakni dengan cara biodegradasi secara mikrobiologi menggunakan bakteri. Penelitian ini bertujuan untuk mengetahui komunitas bakteri pada sampah plastik, khususnya jenis Polyethylene Terephthalate secara molekuler. Untuk analisis bakteri secara molekuker, diperlukan tahapan isolasi DNA bakteri. Pada penelitian ini isolasi DNA bakteri tanpa kultivasi dari sampah plastik dilakukan dengan mengikuti prosedur ekstraksi DNeasy® PowerSoil Pro Kit, Qiagen dengan menggunakan proses pengikisan pada kedua permukaan plastik sebagai tahapan preparasi sampel. Hasil ekstraksi DNA selanjutnya diuji menggunakan elektroforesis gel dan spektofotometer UV-Vis. Genom DNA kemudian diamplifikasi menggunakan Polymerase Chain Reaction dan bakteri diidentifikasi dengan Next Generation Sequencing analysis. Hasil penelitian menujukkan bahwa, DNA bakteri tanpa kultivasi dari sampah plastik berhasil diisolasi dan diamplifikasi yang ditunjukkan dengan terbentuknya pita DNA lewat hasil elektroforesis gel pada panjang 1500 bp. Bakteri yang terdapat pada plastik jenis PET adalah Proteobacteria, Cyanobacteria, Actinobacteria, Chlorobi, Bacteroidetes, Firmicutes, Chloroflexi, Thaumarchaeuta dan Gemmattimonadetes dengan kelimpahan relatif tertinggi adalah ProteobacteriaKata kunci: Bakteri, Deoxyribo Nucleic Acid ( DNA), Sampah Plastik, Tanpa Kultivasi