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KAJIAN PROSES PRODUKSI PUPUK HAYATI BIO-SRF DAN PENGUJIAN EFEKTIVITASNYA PADA TANAMAN BAWANG MERAH Sukmadi, R Bambang; Supriyo, Agus; Rupaedah, Bedah; Mira, Farida Rosana; Bakhtiar, Yenni; Ali, Asep; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 1 (2016): June 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.342 KB) | DOI: 10.29122/jbbi.v3i1.46

Abstract

This study aimed to assess the production process of biofertilizer Bio-SRF and determine its effectiveness on the growth and productivity of shallot plants. The Study of biofertilizer Bio-SRF production covering the cultivation of microbial cell biomass, granulation, and formulation of  biofertilizer products. Testing the effectiveness of biofertilizers on shallot plants using a randomized complete block design (RCBD) with five replicates, involving nine biofertilizers treatments and one control. The results showed that the population of cells on the granulated biofertilizer Bio-SRF was Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3.8 x 107 cfu/g, Burkholderia seminalis 7.4 x 108 cfu/g, Pseudomonas stutzeri 4.5 x 108 cfu/g and 60 mycorrhizal spores/g products. The effectiveness test showed that the biofertilizer treatments significantly affected plant height, the number of bulbs, weight of wet and dried bulbs produced. Application of biofertilizer Bio-SRF on shallot plants gave the best results of plant height 34.80 cm at harvest time, the number of bulbs per plant 4.78 bulb, weight of wet bulbs 3,81 kg/m2, weight of dried bulbs 3,27 kg/m2 and increased the yield of shallot production by 55.71% compared with no biofertilizer application.Keywords: Biofertilizer, Bio-SRF, production process, effectivity test, shallot plant ABSTRAKBio-SRF merupakan formula produk pupuk hayati yang mengandung campuran beberapa jenis mikroba penyubur tanah. Penelitian ini bertujuan untuk mengkaji proses produksi pupuk hayati Bio-SRF dan mengetahui efektivitasnya terhadap pertumbuhan dan produktivitas tanaman bawang merah. Kajian produksi pupuk hayati Bio-SRF meliputi perbanyakan biomassa sel mikroba, granulasi dan formulasi produk. Hasil penelitian menunjukkan bahwa populasi sel pada produk pupuk hayati Bio-SRF bentuk granul adalah Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3,8 x 107 cfu/g, Burkholderia seminalis 7,4 x 108 cfu/g, Pseudomonas stutzeri 4,5 x 108 cfu/g dan mikoriza 60 spora/g produk. Hasil uji efektivitas menunjukkan bahwa perlakuan pupuk hayati berpengaruh nyata terhadap tinggi tanaman, jumlah umbi, bobot basah dan bobot kering umbi bawang merah yang dihasilkan. Aplikasi pupuk hayati Bio-SRF pada tanaman bawang merah memberikan hasil terbaik yaitu dengan tinggi tanaman saat panen 34,80 cm, jumlah umbi per tanaman 4,78 umbi, berat basah umbi 3,81 kg/m2, berat kering umbi 3,27 kg/m2 dan dapat meningkatkan hasil produksi bawang merah sebesar 55,71% dibandingkan dengan tanpa aplikasi pupuk hayati.Kata kunci: Pupuk hayati, Bio-SRF, proses produksi, uji efektivitas, bawang merah
AKTIVITAS FITOHORMON INDOLE-3-ACETIC ACID (IAA) DARI BEBERAPA ISOLAT BAKTERI RIZOSFER DAN ENDOFIT sukmadi, R bambang
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.08 KB) | DOI: 10.29122/jsti.v14i3.930

Abstract

A total of 34 isolates of rhizosphere bacteria and endophytic bacteria have been isolated from several fruit and vegetable plants. Bacterial isolates were then screened to produce phytohormone indole-3-acetic acid (IAA) in a minimal medium (MM) with the addition of 1 g/l tryptophan as a precursor. Phytohormone production was carried out in an incubator shaker for 5 days at 28oC in a dark condition. IAA in the culture supernatant was extracted with ethyl acetate. The presence of IAA in the bacterial extract was detected using Thin Layer Chromatography (TLC). The concentration of IAA was determined quantitatively using High Performance LiquidChromatography (HPLC). The result showed that among 34 bacterial isolates, 20 isolates were able to produce IAA with different concentrations. The highest concentration of IAA (16,71 ppm) was produced by the endophytic bacteria isolated from twigs of starfruit plant. In addition, within the group of rhizosphere bacterial isolates, bacteria from kangkung plant was found to be the best one with IAA concentration of 10.99 ppm.
KAJIAN PROSES PRODUKSI PUPUK HAYATI BIO-SRF DAN PENGUJIAN EFEKTIVITASNYA PADA TANAMAN BAWANG MERAH Sukmadi, R Bambang; Supriyo, Agus; Rupaedah, Bedah; Mira, Farida Rosana; Bakhtiar, Yenni; Ali, Asep; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.342 KB) | DOI: 10.29122/jbbi.v3i1.46

Abstract

This study aimed to assess the production process of biofertilizer Bio-SRF and determine its effectiveness on the growth and productivity of shallot plants. The Study of biofertilizer Bio-SRF production covering the cultivation of microbial cell biomass, granulation, and formulation of  biofertilizer products. Testing the effectiveness of biofertilizers on shallot plants using a randomized complete block design (RCBD) with five replicates, involving nine biofertilizers treatments and one control. The results showed that the population of cells on the granulated biofertilizer Bio-SRF was Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3.8 x 107 cfu/g, Burkholderia seminalis 7.4 x 108 cfu/g, Pseudomonas stutzeri 4.5 x 108 cfu/g and 60 mycorrhizal spores/g products. The effectiveness test showed that the biofertilizer treatments significantly affected plant height, the number of bulbs, weight of wet and dried bulbs produced. Application of biofertilizer Bio-SRF on shallot plants gave the best results of plant height 34.80 cm at harvest time, the number of bulbs per plant 4.78 bulb, weight of wet bulbs 3,81 kg/m2, weight of dried bulbs 3,27 kg/m2 and increased the yield of shallot production by 55.71% compared with no biofertilizer application.Keywords: Biofertilizer, Bio-SRF, production process, effectivity test, shallot plant ABSTRAKBio-SRF merupakan formula produk pupuk hayati yang mengandung campuran beberapa jenis mikroba penyubur tanah. Penelitian ini bertujuan untuk mengkaji proses produksi pupuk hayati Bio-SRF dan mengetahui efektivitasnya terhadap pertumbuhan dan produktivitas tanaman bawang merah. Kajian produksi pupuk hayati Bio-SRF meliputi perbanyakan biomassa sel mikroba, granulasi dan formulasi produk. Hasil penelitian menunjukkan bahwa populasi sel pada produk pupuk hayati Bio-SRF bentuk granul adalah Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3,8 x 107 cfu/g, Burkholderia seminalis 7,4 x 108 cfu/g, Pseudomonas stutzeri 4,5 x 108 cfu/g dan mikoriza 60 spora/g produk. Hasil uji efektivitas menunjukkan bahwa perlakuan pupuk hayati berpengaruh nyata terhadap tinggi tanaman, jumlah umbi, bobot basah dan bobot kering umbi bawang merah yang dihasilkan. Aplikasi pupuk hayati Bio-SRF pada tanaman bawang merah memberikan hasil terbaik yaitu dengan tinggi tanaman saat panen 34,80 cm, jumlah umbi per tanaman 4,78 umbi, berat basah umbi 3,81 kg/m2, berat kering umbi 3,27 kg/m2 dan dapat meningkatkan hasil produksi bawang merah sebesar 55,71% dibandingkan dengan tanpa aplikasi pupuk hayati.Kata kunci: Pupuk hayati, Bio-SRF, proses produksi, uji efektivitas, bawang merah