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Mahmud Sugianto, Mahmud
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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KAJIAN PROSES PRODUKSI PUPUK HAYATI BIO-SRF DAN PENGUJIAN EFEKTIVITASNYA PADA TANAMAN BAWANG MERAH Sukmadi, R Bambang; Supriyo, Agus; Rupaedah, Bedah; Mira, Farida Rosana; Bakhtiar, Yenni; Ali, Asep; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 1 (2016): June 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.342 KB) | DOI: 10.29122/jbbi.v3i1.46

Abstract

This study aimed to assess the production process of biofertilizer Bio-SRF and determine its effectiveness on the growth and productivity of shallot plants. The Study of biofertilizer Bio-SRF production covering the cultivation of microbial cell biomass, granulation, and formulation of  biofertilizer products. Testing the effectiveness of biofertilizers on shallot plants using a randomized complete block design (RCBD) with five replicates, involving nine biofertilizers treatments and one control. The results showed that the population of cells on the granulated biofertilizer Bio-SRF was Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3.8 x 107 cfu/g, Burkholderia seminalis 7.4 x 108 cfu/g, Pseudomonas stutzeri 4.5 x 108 cfu/g and 60 mycorrhizal spores/g products. The effectiveness test showed that the biofertilizer treatments significantly affected plant height, the number of bulbs, weight of wet and dried bulbs produced. Application of biofertilizer Bio-SRF on shallot plants gave the best results of plant height 34.80 cm at harvest time, the number of bulbs per plant 4.78 bulb, weight of wet bulbs 3,81 kg/m2, weight of dried bulbs 3,27 kg/m2 and increased the yield of shallot production by 55.71% compared with no biofertilizer application.Keywords: Biofertilizer, Bio-SRF, production process, effectivity test, shallot plant ABSTRAKBio-SRF merupakan formula produk pupuk hayati yang mengandung campuran beberapa jenis mikroba penyubur tanah. Penelitian ini bertujuan untuk mengkaji proses produksi pupuk hayati Bio-SRF dan mengetahui efektivitasnya terhadap pertumbuhan dan produktivitas tanaman bawang merah. Kajian produksi pupuk hayati Bio-SRF meliputi perbanyakan biomassa sel mikroba, granulasi dan formulasi produk. Hasil penelitian menunjukkan bahwa populasi sel pada produk pupuk hayati Bio-SRF bentuk granul adalah Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3,8 x 107 cfu/g, Burkholderia seminalis 7,4 x 108 cfu/g, Pseudomonas stutzeri 4,5 x 108 cfu/g dan mikoriza 60 spora/g produk. Hasil uji efektivitas menunjukkan bahwa perlakuan pupuk hayati berpengaruh nyata terhadap tinggi tanaman, jumlah umbi, bobot basah dan bobot kering umbi bawang merah yang dihasilkan. Aplikasi pupuk hayati Bio-SRF pada tanaman bawang merah memberikan hasil terbaik yaitu dengan tinggi tanaman saat panen 34,80 cm, jumlah umbi per tanaman 4,78 umbi, berat basah umbi 3,81 kg/m2, berat kering umbi 3,27 kg/m2 dan dapat meningkatkan hasil produksi bawang merah sebesar 55,71% dibandingkan dengan tanpa aplikasi pupuk hayati.Kata kunci: Pupuk hayati, Bio-SRF, proses produksi, uji efektivitas, bawang merah
AKTIVITAS Stenotrophomonas rhizophila DAN Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Ganoderma boninense Rupaedah, Bedah; Amanda, Debby Viola; Indrayanti, Reni; Asiani, Nia; Sukmadi, Bambang; Ali, Asep; Wahid, Abdul; Firmansyah, Taufik; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3510.05 KB) | DOI: 10.29122/jbbi.v5i1.2767

Abstract

Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninense ABSTRACTBasal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp. ABSTRAKPenyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila,    Trichoderma sp.
KAJIAN PROSES PRODUKSI PUPUK HAYATI BIO-SRF DAN PENGUJIAN EFEKTIVITASNYA PADA TANAMAN BAWANG MERAH Sukmadi, R Bambang; Supriyo, Agus; Rupaedah, Bedah; Mira, Farida Rosana; Bakhtiar, Yenni; Ali, Asep; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.342 KB) | DOI: 10.29122/jbbi.v3i1.46

Abstract

This study aimed to assess the production process of biofertilizer Bio-SRF and determine its effectiveness on the growth and productivity of shallot plants. The Study of biofertilizer Bio-SRF production covering the cultivation of microbial cell biomass, granulation, and formulation of  biofertilizer products. Testing the effectiveness of biofertilizers on shallot plants using a randomized complete block design (RCBD) with five replicates, involving nine biofertilizers treatments and one control. The results showed that the population of cells on the granulated biofertilizer Bio-SRF was Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3.8 x 107 cfu/g, Burkholderia seminalis 7.4 x 108 cfu/g, Pseudomonas stutzeri 4.5 x 108 cfu/g and 60 mycorrhizal spores/g products. The effectiveness test showed that the biofertilizer treatments significantly affected plant height, the number of bulbs, weight of wet and dried bulbs produced. Application of biofertilizer Bio-SRF on shallot plants gave the best results of plant height 34.80 cm at harvest time, the number of bulbs per plant 4.78 bulb, weight of wet bulbs 3,81 kg/m2, weight of dried bulbs 3,27 kg/m2 and increased the yield of shallot production by 55.71% compared with no biofertilizer application.Keywords: Biofertilizer, Bio-SRF, production process, effectivity test, shallot plant ABSTRAKBio-SRF merupakan formula produk pupuk hayati yang mengandung campuran beberapa jenis mikroba penyubur tanah. Penelitian ini bertujuan untuk mengkaji proses produksi pupuk hayati Bio-SRF dan mengetahui efektivitasnya terhadap pertumbuhan dan produktivitas tanaman bawang merah. Kajian produksi pupuk hayati Bio-SRF meliputi perbanyakan biomassa sel mikroba, granulasi dan formulasi produk. Hasil penelitian menunjukkan bahwa populasi sel pada produk pupuk hayati Bio-SRF bentuk granul adalah Corynebacterium sp. 4 x 107 cfu/g, Lactobacillus sp. 3,8 x 107 cfu/g, Burkholderia seminalis 7,4 x 108 cfu/g, Pseudomonas stutzeri 4,5 x 108 cfu/g dan mikoriza 60 spora/g produk. Hasil uji efektivitas menunjukkan bahwa perlakuan pupuk hayati berpengaruh nyata terhadap tinggi tanaman, jumlah umbi, bobot basah dan bobot kering umbi bawang merah yang dihasilkan. Aplikasi pupuk hayati Bio-SRF pada tanaman bawang merah memberikan hasil terbaik yaitu dengan tinggi tanaman saat panen 34,80 cm, jumlah umbi per tanaman 4,78 umbi, berat basah umbi 3,81 kg/m2, berat kering umbi 3,27 kg/m2 dan dapat meningkatkan hasil produksi bawang merah sebesar 55,71% dibandingkan dengan tanpa aplikasi pupuk hayati.Kata kunci: Pupuk hayati, Bio-SRF, proses produksi, uji efektivitas, bawang merah
AKTIVITAS Stenotrophomonas rhizophila DAN Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Ganoderma boninense Rupaedah, Bedah; Amanda, Debby Viola; Indrayanti, Reni; Asiani, Nia; Sukmadi, Bambang; Ali, Asep; Wahid, Abdul; Firmansyah, Taufik; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3510.05 KB) | DOI: 10.29122/jbbi.v5i1.2767

Abstract

Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninense ABSTRACTBasal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp. ABSTRAKPenyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila,    Trichoderma sp.
SKRINING DAN IDENTIFIKASI MIKROBA LIGNINOLITIK PADA PENGOMPOSAN ALAMI TANDAN KOSONG KELAPA SAWIT Rupaedah, Bedah; Purwoko, Devit; Safarrida, Anna; Tajuddin, Teuku; Wahid, Abdul; Sugianto, Mahmud; Sudjai, Imam; Suyono, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (713.706 KB) | DOI: 10.29122/jbbi.v6i1.3237

Abstract

Screening and Identification of Ligninolytic Microbes in the Natural Decomposition of Oil Palm Empty Fruit Bunch  ABSTRACTOPEFB (oil palm empty fruit bunch)could potentially be utilized as organic fertilizer or animal feed through composting. Information on microorganisms that play important roles in the natural decomposition of OPEFB is to date not much known yet. This research was aimed to obtain and, subsequently, to molecularly identify lignin-degrading microbial isolates responsible for naturally decomposing OPEFB in the Oil Plant Plantation and Palm Oil Refinery Plant, PTPN VIII Cikasungka, Bogor. Screening for active lignin-degrading isolates was carried out on 17 naturally decomposing OPEFB samples. A total of 19 isolates of fungi and 80 isolates of bacteria were obtained. Ligninolytic activity was measured by Sundman and Nase testing methods. Ligninolytic activity was found on 13 fungal isolates and 15 bacterial isolates. The active isolates were subsequently identified molecularly based on ITS sequence in the ribosome DNA area for fungi and in 16S rRNA genes for bacteria. The results showed that the lignin-degrading microorganisms obtained consisted of 5 bacterial isolates from the genus Bacillus and 3 fungal isolates from the genus Rhizopus and Aspergillus. Keywords: composting, lignin, microbes, OPEFB, 16S rRNA ABSTRAKTKKS (tandan kosong kelapa sawit) berpotensi dimanfaatkan sebagai pupuk organik atau pakan ternak dengan cara pengomposan. Informasi mikroba yang berperan dalam pengomposan alami TKKS hingga saat ini belum banyak diketahui. Penelitian ini bertujuan mendapatkan isolat mikroba pendegradasi lignin dalam pengomposan alami TKKS asal Perkebunan dan Pabrik Pemerasan Kelapa Sawit, PTPN VIII Cikasungka, Bogor, serta mengidentifikasi mikroba tersebut secara molekuler. Skrining mikroba aktif pendegradasi lignin dilakukan terhadap 17 sampel TKKS yang sudah lapuk secara alami. Sebanyak 19 isolat jamur dan 80 isolat bakteri telah dihasilkan. Aktivitas ligninolitik diukur dengan metode pengujian Sundman dan Nase. Isolat jamur yang memiliki aktivitas ligninolitik sebanyak 13 isolat, sedangkan bakteri sebanyak 15 isolat. Isolat-isolat aktif tersebut selanjutnya diidentifikasi secara molekuler berdasarkan pada sekuen ITS di daerah DNA ribosom untuk jamur dan menggunakan gen 16S rRNA untuk bakteri. Hasil menunjukkan bahwa 5 isolat bakteri yang memiliki kemampuan mendegradasi lignin berasal dari genus Bacillus, sedangkan 3 isolat jamur pendegradasi lignin berasal dari genus Rhizopus dan Aspergillus Kata Kunci: lignin, mikroba, pengomposan, TKKS, 16S rRNA 
Co-Authors A.A. Ketut Agung Cahyawan W Abd. Rasyid Syamsuri Agus Supriyo, Agus AGUS SUYONO Amanda, Debby Viola Amanda, Debby Viola Anna Safarrida, Anna Asep Ali, Asep Asiani, Nia Asiani, Nia Bedah Rupaedah, Bedah Farida Rosana Mira, Farida Rosana Firmansyah, Taufik Firmansyah, Taufik purwoko, devit R Bambang Sukmadi, R Bambang Reni Indrayanti, Reni Sudjai, Imam Sukmadi, Bambang Sukmadi, Bambang Teuku Tajuddin YENNI BAKHTIAR