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The Effect of Hyperbaric Oxygen Therapy to the Amount of Lymphocytes in Oral Candidiasis Immunosuppressed Model Agni Febrina Pargaputri; Dwi Andriani
Denta Journal Kedokteran Gigi Vol 12 No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. Purpose: To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. Methods: This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. Results: The data were analyzed with one way Anova test and showed significant difference among groups (p<0,05). To show the difference between each groups we used LSD test and showed significant difference (p<0,05) between K+ compared with P1, and K+ compared with K-. Conclusion: Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models.

The Effect of Brown Algae Sargassum sp. Extract Towards The Amount of Macrophages in The Healing Process of Traumatic Ulcer Annisa Rahmawati; Agni Febrina Pargaputri; Isidora Karsini S
Denta Journal Kedokteran Gigi Vol 12 No 1 (2018): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: The Sarggasum’s sp. extract contains of several chemical properties such as flavonoid, vitamin A, vitamin C, zinc, and calcium which has benefit as anti-inflammation, so it can be used as an alternative therapy for traumatic ulcer. Purpose: To investigate the effectivity of Sargassum’s sp. extract towards the amount of macrophages in the healing process of traumatic ulcers. Material and Method: 32 wistar rats were divided into 4 groups, K1 the negative control group without treatment, K2 the positive control group with the drugs containing 0.2% hyaluronic acid, and the treatment group K3 Sargassum’s sp. extract concentrations of 50% and K4 Sargassum’s sp. extract concentrations of 75%. Traumatic ulcer were made in the labial mucosa of rats by using heated amalgam stopper. On the fourth and seventh day, rats were sacrified and biopsied on lower labial mucosa. The macrophages were observed using a 1000x magnification light microscope with OptiLab viewer. Result: The statistical test using One Way ANOVA showed significant difference (p<0,05) of macrophage on fourth day group. The LSD test showed a significant difference (p<0,05) in macrophages at K1 (2.83±1.03) compared to K3 (1.17±0.64), K1 compared to K4 (0.58±0.79), K2 (2.58±0.50) compared to K3, and K2 compared to K4 at the fourth day group, whereas the seventh day group showed no significant differences between each groups. Conclusion: The Sargassum’s sp. extract concentrations of 75% is the most effective in decreasing amount of macrophages in the healing process of traumatic ulcers.

The Effect of Pluchea indica Less Leaves Extract Againts Biofilm of Enterococcus faecalis and Fusobacterium nucleatum In Vitro Agni Febrina Pargaputri; Elly Munadziroh; Retno Indrawati
Denta Journal Kedokteran Gigi Vol 11 No 1 (2017): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Enterococcus faecalis and Fusobacterium nucleatum are the most common bacteria found in infected root canal teeth and most of them often caused failure in endodontic treatments. These bacteria can form biofilm which makes them more resistant against antibacterial agents. Biofilm formation also causes a decrease in antibiotics and antimicrobials sensitivity. Pluchea indica Less leaves is a species of plants that has several chemical properties. It consists of flavonoids and polyphenols which have benefits to inhibit biofilm formation. Because of its benefits, the extract of Pluchea indica Less leaves can be potentially developed as one of sterilization dressing in root canal teeth. Purpose: The aim of this study was to determine biofilm formation inhibition of Pluchea indica Less leaves extract against Enterococcus faecalis and Fusobacterium nucleatum. Materials and Methods: The dilution method was done first to show the Minimum Inhibitory Concentration (MIC) of the extract. The inhibition biolfilm formation was tested using microtitter plate assay by measuring the bacterial biofilm Optical Density (OD) from ELISA reader’s results and using autoagregation assay to show the inhibition of adherance bacteria. The Pluchea indica Less leaves extract concentration used for inhibition of biofilm formation were 100%, 50%, 25%, 12,5%, and 6,25%. Results: The result of biofilm formation inhibition showed that Pluchea indica Less leaves extract were able to inhibit Enterococcus faecalis and Fusobacterium nucleatum’ biofilm formation with strong moderate effect. The autoagregation assay showed a decrease in autoagregation percentation of Enterococcus faecalis and Fusobacterium nucleatum. Conclusions: Pluchea indica Less leaves extract has effect to inhibit biofilm formation of Enterococcus faecalis and Fusobacterium nucleatum.

Daya Antibakteri Ekstrak Daun Beluntas (Pluchea Indica Less) Terhadap Streptococcus Viridans (In Vitro) Agni Febrina Pargaputri; M. Mudjiono; Agus Subiwahjudi
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Pluchea indica Less leaves is a species of plants that has several chemical properties. It consists of flavonoids, tanine, and essensial oil which had been reported as antibacterial agents. Streptococcus viridans is the most common bacteria found in infected root canal teeth, and the extract of Pluchea indica Less leaves could be potentially used as one of root canal sterilization dressing in root canal teeth. Purpose: The aim of this study was to determine antibacterial activity and Minimum Bactericidal Concentration (MBC) of Pluchea indica Less leaves extract against Streptococcus viridans. Materials and Methods: The antibacterial testing of Pluchea indica Less leaves extract was performed using dilution method against Streptococcus viridans. The tube with concentration extract of 25% was showed no visible turbidity, while the tube with concentration extract of 12,5% was showedgrowth of Streptococcus viridans. We made range between concentration 12,5% until 25% to determine the Minimum Bactericidal Concentration (MBC) of Pluchea indica Less leaves extract. The result of antibacterial activity of Pluchea indica Less leaves extract was determined by measure the growth of Streptococcus viridans using colony count. Result: The result showed that Pluchea indica Less leaves extract was able to inhibit the growth of Streptococcus viridans on concentration 12,5%-25%. The results of Anova statistical test (significant value <0,05) showed significant different among each concentration of Pluchea indica Less leaves extract. Conclusion: Pluchea indica Less leaves extract had antibacterial activity against Streptococcus viridans. Minimum Bactericidal Concentration of Pluchea indica Less leaves extract was performed in concentration 12,5%-25%, which was could kill more than 99% growth of Streptococcus viridans.

Effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats Agni Febrina Pargaputri; Dwi Andriani; Mardiyanto Riski Hartono; Kharinna Widowati
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.2.319-326

Background: Liver is an organ that performs a role in metabolism and detoxification of chemical substances. The use of immunosuppressive drugs excessively not only become predisposing factor for oral candidiasis, but also can cause damage and affect liver’s function. Hyperbaric oxygen treatment was known to preserve hepatocytes and prevent liver damage. This study was aimed to investigate the effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats.Method: We divided fifteen Wistar rats randomly into three groups: G1 (healthy rats), G2 (immunosuppressed rats with oral candidiasis), and G3 (immunosuppressed rats with oral candidiasis given hyperbaric oxygen). The immunosuppressed condition was made by giving dexamethasone and tetracycline orally for 14 days. Induction of Candida albicans 0.1 cc was conducted on the fourth day. Hyperbaric oxygen treatment was given for five days continuously. The histopathological changes in the liver were measured by counting the amount of normal hepatocytes, pycnotic, karyolysis, karyorrhexis, and score of necrosis area using light microscope with 400x magnification.Result: The amount of pycnotic, karyolysis, and karyorrhexis hepatocytes increased in G2 and decreased significantly in G3, likewise the score of necrosis area. The statistical test using oneway Anova and LSD test showed significant differences (p<0,05) in pycnotic, karyolysis, and karyorrhexis hepatocytes between G1, G2, and G3.Conclusion: The hyperbaric oxygen treatment effect on liver hepatocyte damage in oral candidiasis immunosuppressed rats.

Pendidikan Kesehatan Gigi dan Mulut Melalui Media Edukasi Pahlawan Gigi (PAGI) di KB Taam Avicenna Kelurahan Sukolilo Baru Surabaya Agni Febrina Pargaputri; Aulia Dwi Maharani; Fauziah Julike Patrika
Jurnal Abdi Masyarakat Indonesia Vol 3 No 2 (2023): JAMSI - Maret 2023
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.715

Pendidikan kesehatan gigi dan mulut menjadi bagian penting dalam penerapan perilaku menjaga kesehatan gigi yang sebaiknya diberikan sejak usia dini, karena akan mempengaruhi tingkat kesehatan gigi dan rongga mulut, serta dapat mempertahankan fungsi gigi geligi hingga dewasa nanti. Dalam penyampaian pendidikan kesehatan gigi dan mulut pada anak tingkat sekolah, diperlukan media edukasi yang menarik, komunikatif, dan demonstratif sehingga dapat memberi kesan yang mendalam pada daya ingat siswa, dan mampu diterapkan dengan baik. Kegiatan ini bertujuan untuk memberikan pendidikan kesehatan gigi dan mulut dengan menggunakan media edukasi Pahlawan Gigi (PAGI) di Kelompok Bermain Taman asuh anak muslim Avicenna Kelurahan Sukolilo Baru Surabaya. Kegiatan ini dilakukan dengan memberikan penyuluhan dan mempraktekkan cara menjaga kebersihan gigi dan mulut dengan benar. Evaluasi dilakukan dengan memberikan pertanyaan pada siswa sebelum dan sesudah materi edukasi kesehatan gigi diberikan. Evaluasi terhadap perilaku menggosok gigi dilakukan dengan melihat chart menggosok gigi pada tiap siswa. Pemberian pendidikan kesehatan gigi dan mulut dengan media edukasi Pahlawan Gigi menunjukkan bertambahnya pengetahuan siswa terhadap cara menjaga kebersihan dan kesehatan gigi, serta adanya perbaikan perilaku menggosok gigi yang dilakukan siswa di rumah menjadi lebih baik.

Effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats Agni Febrina Pargaputri; Dwi Andriani; Mardiyanto Riski Hartono; Kharinna Widowati
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Liver is an organ that performs a role in metabolism and detoxification of chemical substances. The use of immunosuppressive drugs excessively not only become predisposing factor for oral candidiasis, but also can cause damage and affect liver’s function. Hyperbaric oxygen treatment was known to preserve hepatocytes and prevent liver damage. This study was aimed to investigate the effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats.Method: We divided fifteen Wistar rats randomly into three groups: G1 (healthy rats), G2 (immunosuppressed rats with oral candidiasis), and G3 (immunosuppressed rats with oral candidiasis given hyperbaric oxygen). The immunosuppressed condition was made by giving dexamethasone and tetracycline orally for 14 days. Induction of Candida albicans 0.1 cc was conducted on the fourth day. Hyperbaric oxygen treatment was given for five days continuously. The histopathological changes in the liver were measured by counting the amount of normal hepatocytes, pycnotic, karyolysis, karyorrhexis, and score of necrosis area using light microscope with 400x magnification.Result: The amount of pycnotic, karyolysis, and karyorrhexis hepatocytes increased in G2 and decreased significantly in G3, likewise the score of necrosis area. The statistical test using oneway Anova and LSD test showed significant differences (p<0,05) in pycnotic, karyolysis, and karyorrhexis hepatocytes between G1, G2, and G3.Conclusion: The hyperbaric oxygen treatment effect on liver hepatocyte damage in oral candidiasis immunosuppressed rats.

Dental and oral health education in parents of taam avicenna playgroup students Agni Febrina Pargaputri; Aulia Dwi Maharani; Fauziah Julike Patrika
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol. 6 No. 3 (2022)
Publisher : Universitas Ahmad Dahlan

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Oral and dental health education aims to increase knowledge that leads to better dental and oral health behaviors. Parents play an important role in children's behavior to maintain dental and oral health. Lack of behavior to maintain healthy teeth can be caused by low knowledge of dental and oral health. This activity aim to provide dental and oral health education to parents of students in Taam Avicenna Surabaya playgroup. This activity was carried out by providing dental health education media such as flipcharts, posters, and dental phantoms. Then post-test was given to the participants. Questionnaires regarding the behavior of maintaining dental and oral health were also given. The results showed that the understanding of parents with good categories was more than those with moderate and low categories. This means that some parents can receive dental health education well. Providing dental health education has a positive impact on understanding and knowledge of dental and oral health in parents. It can be concluded that by giving dental health education for parents of students in Taam Avicenna Surabaya playgroup, could enhance understanding and knowledge of dental and oral health so that it could help parents to improve behavior in oral and dental health.

Pengaruh nystatin topikal dan terapi oksigen hiperbarik terhadap jumlah makrofag pada tikus kandidiasis oral kondisi imunosupresi: studi eksperimental laboratoris Agni Febrina Pargaputri; Dwi Andriani; Dian Mulawarmanti; Ivanda Rizka Amaliah
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 2 (2023): Agustus 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i2.47145

ABSTRAKPendahuluan: Kandidiasis oral adalah infeksi jamur di mukosa mulut yang sebagian besar disebabkan oleh Candida albicans (C.albicans). Kondisi imunosupresi dapat menyebabkan penurunan sistem imun tubuh, sehingga menurunkan kemampuan melawan C. albicans. Pemberian obat anti jamur nystatin telah diketahui dapat menurunkan infeksi kandidiasis oral, selain itu terapi adjuvant menggunakan oksigen hiperbarik dapat memicu aktivasi makrofag dan menurunkan sitokin proinflamatori. Penelitian ini bertujuan untuk menganalisis pengaruh nystatin topikal dan terapi oksigen hiperbarik terhadap jumlah makrofag pada tikus kandidiasis oral dengan kondisi imunosupresi. Metode: Jenis penelitian eksperimental laboratoris. Tikus Wistar jantan sejumlah 20 dibagi menjadi 4 kelompok: P1 (normal/sehat), P2 (tikus kandidiasis oral kondisi imunosupresi), P3 (tikus kandidiasis oral kondisi imunosupresi diberi nystatin topikal), P4 (tikus kandidiasis oral kondisi imunosupresi diberi terapi oksigen hiperbarik). Imunosupresi dilakukan dengan memberikan deksametason secara peroral selama 14 hari dengan dosis 0,5 mg/hari/tikus, ditambahkan tetrasiklin 1 mg/hari/tikus. Induksi C. albicans dilakukan pada dorsum lidah, dua hari sekali selama 12 hari. Nystatin topikal dan terapi oksigen hiperbarik 2,4 Atmosfer Absolut (ATA) diberikan selama 5 hari. Jumlah makrofag pada sediaan lidah tikus diamati menggunakan mikroskop cahaya dengan perbesaran 1000x. Analisis data menggunakan uji Anova dan Least Significance Different (LSD). Hasil: Uji Anova menunjukkan terdapat perbedaan signifikan (p < 0,05) antara kelompok penelitian. Analisis statistik LSD menunjukkan terdapat perbedaan signifikan (p < 0,05) antara kelompok P1 dengan P2 dan P3, serta kelompok P2 dengan P3 dan P4, sedangkan antara kelompok P1 dan P4, serta P3 dan P4, tidak terdapat perbedaan yang bermakna (p > 0,05). Simpulan: Pemberian nystatin topikal dan terapi oksigen hiperbarik berpengaruh pada jumlah makrofag tikus kandidiasi oral dengan kondisi imunosupresi.Kata kunciimunosupresi, kandidiasis oral, makrofag, nystatin topikal, terapi oksigen hiperbarik The effect of topical nystatin and hyperbaric oxygen therapy towards number of macrophages in oral candidiasis rats with immunosuppressed condition: experimental laboratory studyABSTRACT Introduction: Oral candidiasis is a fungal infection in oral mucosa, mostly caused by Candida albicans (C. albicans). Immunosuppressive conditions can cause a decrease in the immune system, thereby reducing the ability to fight C. albicans. Nystatin has known can decrease oral candidiasis infection, furthermore an adjuvant therapy using hyperbaric oxygen can trigger the activation of macrophages and decrease proinflammatory cytokines. The aim of this study was to analyze the effect of topical nystatin and hyperbaric oxygen therapy towards the number of macrophages in oral candidiasis immunosuppressed rats. Methods: 20 male Wistar rats were divided into 4 groups: P1 (normal/ healthy), P2 (Oral candidiasis immunosuppressed rats,) P3 (Oral candidiasis immunosuppressed rats with topical nystatin), and P4 (Oral candidiasis immunosuppressed rats with hyperbaric oxygen therapy). Immunosuppressed condition was made by giving dexamethasone 0.5 mg/day orally, added with tetracycline 1 mg/day for 14 days. After 3 days, rats were induced with C. albicans once in two days for 12 days. Topical nystatin and hyperbaric oxygen therapy 2,4 ATA were given for 5 days. The number of macrophages were observed using microscope at 1000x magnification. The data were analyzed using Anova and LSD tests. Results: Anova test showed a significant difference (p < 0.05) between groups. LSD analysis showed a significant difference (p < 0.05) between P1, P2, and P3, also P2 with P3 and P4, while there was no significant difference (p > 0.05) between P1 and P4, also P3 and P4. Conclusion: Topical nystatin and hyperbaric oxygen therapy have effect into the number of macrophages in oral candidiasis immunosuppressed rats.Key wordsimmunosuppression, oral candidiasis, macrophages, topical nystatin, oral candidiasis, hyperbaric oxygen therapy

Peningkatan Derajat Kesehatan Gigi dan Mulut Siswa SD IT Al Uswah melalui Program Training of Traniner (ToT) Ghita Hadi Hollanda; Diana Soesilo; Aulia Dwi Maharani; Agni Febrina Pargaputri; Ari Rosita Irmawati; Yufita Fitriani; Restika Anindya Pinasti; Bunga Fauzia; Moh. Basroni Rizal
Jurnal Pengabdian Masyarakat Pesisir VOLUME 2 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v2i2.95

Kesehatan gigi dan mulut di Indonesia masih merupakan masalah kesehatan yang perlu diperhatikan terutama pada anak-anak usia sekolah dasar. Untuk mengatasi permasalahan kesehatan gigi dan mulut di Indonesia dan menunjang program “Indonesia Bebas Karies 2030”, khususnya pada kelompok masyarakat usia sekolah dan dewasa, Persatuan Dokter Gigi Indonesia (PDGI) dan FDI World Dental Federation akan melakukan kegiatan promotif-preventif. Kegiatan tersebut dinamai “Program Sekolah 21 hari Brush Day and Night”. Program ini dilakukan pada 110 siswa-siswi SD IT Al Uswah Surabaya dengan beberapa kegiatan yang dilakukan diantaranya yaitu edukasi kesehatan gigi dan mulut, sikat gigi bersama, pemeriksaan kesehatan gigi dan mulut serta pemberian topical aplikasi fluor sebagai pencegahan terhadap karies pada gigi. Hasil dari pemeriksaan kesehatan gigi dan mulut menunjukkan bahwa siswa-siswi SD IT Al Uswah Surabaya memiliki indeks def-t kategori tinggi sehingga perlu dilakukan tindakan promotif-preventif dan juga mengadakan training of trainer ke guru dan orang tua agar bisa mengajarkan kebiasaan menjaga kebersihan rongga mulut. Hasil akhir dari program ini diharapkan dapat mencegah meningkatnya derajat kesehatan gigi dan mulut SD IT Al Uswah Surabaya.

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