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Denny Juraijin
IKesT Muhammadiyah Palembang
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Public Health

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PERBANDINGAN PEMERIKSAAN WIDAL DAN SALMONELLA IgG/ IgM PADA PASIEN SUSPEK DEMAM TIFOID Nurhidayanti; Denny Juraijin; Rafli Wilendra
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.3

Abstract

Introduction: Typhoid fever is still a very important health problem. This disease is still often found in developing countries located in tropical areas such as Indonesia and subtropical areas. Acute typhoid fever is an acute systemic infectious disease caused by the microorganism Salmonella enteric serotype typhi known as Salmonella typhi (S. typhi). Laboratory examinations to establish the diagnosis of typhoid fever in general can be checked with the Widal test and Salmonella IgG/IgM, tubex test and culture test. Several diagnostic methods are faster, easier to perform and affordable for developing countries with fairly good sensitivity and specificity, such as Widal and salmonella IgG/IgM tests, starting to be used in Indonesia. Purpose: This study was to determine the examination of Widal and salmonella IgG/IgM in patients with suspected typhoid fever.. Research Method: The type of research used was cross sectional, this research was conducted in the Laboratory of Bunda Palembang Hospital. Samples were serum/plasma of patients with suspected typhoid fever. Results: The results showed that there were 11 patients with suspected typhoid fever who were Widal positive and then confirmed using salmonella IgG/IgM and 6 samples were diagnosed positive with typhoid fever. Data analysis using chi-square test obtained p value = 0.014. Conclusion: The conclusion of this study is that there are significant differences in the examination of Widal and salmonella IgG/IgM in patients with suspected typhoid fever
PERBANDINGAN JUMLAH LEUKOSIT YANG DIHITUNG MENGGUNAKAN MODIFIKASI AIR PERASAN BUAH JERUK KUNCI (Citrus microcarpa Bunge) DAN LARUTAN TURK Aristoteles; Denny Juraijin
jurnal kesehatan terapan sains dan teknologi Vol 1 No 2 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i2.11

Abstract

Background : Laboratory tests, especially hematology, are frequently requested by doctors to help make a diagnosis. White blood cells (leukocytes) are an important part of the body’s defense system whose function is to fight infection causing microorganisms, tumor cells, and harmful foreign substances. Counting the number of leukocytes by manual method is done by diluting the blood using Tur ation, but the problem is that sometimes the Turk solution is not available. or is available but has expired, so an alternative is done by modifying key orange. juice (Citrus macrocarpa Bunge). Research purposes : To determine the difference in the number of leukocytes counted using modified key lime juice (Citrus microcarpa Bunge) and Turk solution. Research Methods : Experimental this research was conducted on January 19, 2023 at Bunda Hospital Palembang. and sampling was carried out at the IkeST Muhammadiyah Palembang Hematology Laboratory, totaling 31 EDTA blood samples. Results : The results obiained in this study showed that there was a difference in the leukocyte count with the modification of key orange juice (Citrus macrocarpa Bunge), turk solution, and a hematology analyzer. The results of the research data were carried out using data analysis with the SPSS program with the Shapiro–Wilk normality test and continued with the Kruskal test. Conclusion : There is a difference between the leukocyte count examination using modified key lime juice (Citrus macrocarpa Bunge) and Turk solution.
Perbedaan Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Konsentrasi Suspensi Sel 5% Segera Periksan Dengan Lama Penyimpanan 5 Hari Dewi Hartati; Hanny Rizky Ananda; Denny Juraijin
jurnal kesehatan terapan sains dan teknologi Vol 2 No 1 (2024): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v2i1.32

Abstract

Background: ABO b;ood group examination is an examination carried out to determinate the type of blood group in humans that detects the presence of antigens on the surface of the red blood cell membrane by means of the Tube Method various consentrations of erythrocyte cell suspension,namely 5%, 10% and 15%. Research purpose: To determinate the effect of various consentrations of erythrocyte cell suspension with ready-to-use 0,9% NaCl and 0,9% table salt solvent on the degree of agglutination in blood group examination Tube method. Research method: This research is a pure experimental study with a total sample 41 people, this study was conducted on January 16-20, 2023 at the Muhammadiyah Palembang IKesT Clinical Pathology Laboratory. Results: The results showed that the blood group examination was done by the tube method with a concentration of erythrocyte cell suspension, namely 5%, 10% and 15% with 0,9% NaCl solvent ready-to-use and 0,9% NaCl, table salt solvent resulting in a degree of agglutination of +4. The Kruskal-wallis test result in the Cell Grouping method obtained 0,000 and in the Serum Grouping method obtained 0,051. Conclusion: The conclusion in this study is that there is no effect of various consentrations of erythrocyte cell suspension with ready-to-use 0,9% NaCl and 0,9% NaCl Solvent Kitchen Salt on the degree of agglutination in blood group examination Tube Method so that 0,9% NaCl solvent from table salt can be used as an alternative to ready-to-use 0,9% NaCl reagent in blood group tests by tube method. Keywords: Blood type, Aglutination
PERBEDAAN HASIL BAKTERI TAHAN ASAM METODE ZIEHL NEELLSEN DAN TES CEPAT MOLEKULER (TCM) DENGAN LAMA PENYIMPANAN 48 JAM Aristoteles; Yuliana Maya Sari; Denny Juraijin
jurnal kesehatan terapan sains dan teknologi Vol 2 No 1 (2024): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v2i1.33

Abstract

Background: Tuberculosis (TBC) is an infectious disease caused by Mycobacterium tuberculosis, a dorect human-to-human infectious disease not caused by witchcraft or curses TB germ most often attack the lungs but can also attack other organs such as the lymph nodes, bones, brain, skin and others. The diagnosis of pulmonary TB is established based on the clinical picture, physical examination, radiological picture, laboratory examination and tuberculin test. The diagnosis of pulmonary tuberculosis currently used routinely in labpratories including hospitals and health centers is a bacteriological diagnosis using acid-fast bacillus microscopic techniques (BTA) and molecular rapid test (TCM). TCM examination is a molecular detection methode based on nested real-time PCR. The use of TCM is a priority for TB examination because it has several advantages, including: High sensitivity, fast and results can be known in approximately 2 hours. Objectives: To determine differences in the results of acid-fast bacteria Ziehl Neelsen method and the molecular rapid test (TCM) with a storage time of 48 hours. Method: This type of research is pure experiment. The research was conducted in the Celikah Health Center laboratory in February 2023 with a total sample of 5 smear samples (+1). Result: The results showed that there was no difference in the tesults of acid-fast bacteria between the ziehl neelsen method and the molecular rapid test (TCM) for 48 hours of storage. The wilcoxon test results obtained a value of p = 0,157. Conclusion: The molecular rapid test method has better sensitivity and specificity in the diagnosis of tuberculosis thab microscopic examination using ziehl neelsen staining.
Co-Authors Aristoteles Dewi Hartati Hanny Rizky Ananda Nurhidayanti Rafli Wilendra Yuliana Maya Sari