Yellow pumpkin (Cucurbita moschata) is an agricultural product that is rich in beta carotene or provitamin A, so it is very good for health. This study aims to determine the effect of storage duration on the germination of yellow pumpkin seeds and was carried out in Bandung from 24 November to 15 December 2022. The experiment was carried out by treating S0=seeds not stored, S1=seeds stored for 1 day, S2 = seeds stored for 3 days, S3 = seeds stored for 5 days, and S4 = seeds stored for 7 days. Each treatment was repeated 3 times with a total of 10 seeds in each repetition. Seeds are planted in soil media as deep as 0.5 cm in a clear plastic container without being covered. What was observed was the germination percentage in each experiment. The results obtained from the experiment showed that seed germination duration had an effect on the germination percentage of yellow pumpkins. The highest percentage of seed germination was achieved in the 5-day storage treatment. Keywords: Cucurbita moschata, yellow pumpkin, germination, storage Abstrak Labu kuning (Cucurbita moschata) merupakan salah satu hasil pertanian yang kaya akan beta karoten atau provitamin A sehingga sangat baik untuk kesehatan. Penelitian ini memiliki tujuan untuk mengetahui pengaruh lama penyimpanan terhadap daya perkecambahan biji labu kuning dan telah dilakukan di Bandung mulai tanggal 24 November hingga 15 Desember 2022. Percobaan dilakukan dengan memberikan perlakuan pada S0=biji tidak disimpan, S1= biji disimpan selama 1 hari, S2= biji disimpan selama 3 hari, S3=biji disimpan selama 5 hari, dan S4=biji disimpan selama 7 hari. Masing-masing perlakuan dilakukan ulangan sebanyak tiga kali dengan jumlah 10 biji pada setiap pengulangan. Biji ditanam pada media tanah sedalam 0,5 cm yang berada di dalam wadah plastik bening tanpa ditutup. Hal yang diamati adalah persentase daya perkecambahan pada setiap percobaan. Hasil yang didapat dari percobaan menunjukkan bahwa lama penyimpanan biji berpengaruh terhadap daya perkecambahan labu kuning. Persentase daya perkecambahan biji tertinggi dicapai pada perlakuan penyimpanan biji selama 5 hari.Kata kunci: Cucurbita moschata, labu kuning, perkecambahan, penyimpanan.