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All Journal Animal Production : Indonesian Journal of Animal Production
RI Arifiantini, RI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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 1 
The Viability of Local Ram Semen in Tris Buffer With Three Different Egg Yolks Nalley, WMM; Arifiantini, RI
ANIMAL PRODUCTION Vol 13, No 1 (2011): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Egg yolk consisted of lecithin and phospholipids are one of the most commonly used components that will protect spermatozoa against cold shock during cooling and freezing.  The aim of this study was to evaluate the effect of different hen egg yolk on Tris extender on the freezability of local ram semen. Semen from six sexually matured local rams was collected weekly using artificial vagina. Collected semen was evaluated macroscopically and microscopically and extended  using tris extender consisted of 20% (v/v) regular  egg yolk (TRCEY), native egg yolk (TNCEY), omega-3 hen egg yolk (TOEY) and 6% (v/v) glycerol. Those were packed in 0.25 ml straws, equilibrated at 5oC for 3 hours, frozen and stored in nitrogen tank for 24 hours, and thawed at 37oC for 30 second. The result of the experiment showed that there were no significant differences on the sperm motility and the number of living sperm. Percentage of plasma membrane intact in TOEY (60.3%) was significantly higher compared to TREY (56.9%) and TNEY (55.6%). In conclusion, the addition of omega 3 egg yolk in Tris extender protects plasma membrane better than the regular or native hen egg yolk. (Animal Production 13 (1):39-44 (2011) Key Words: ram semen, egg yolk, frozen semen
The Viability of the Stallion Spermatozoa after Centrifugation with Different Level of Seminal Plasma in Skim Milk Extender Arifiantini, RI; Yusuf, T Laswardi; Purwantara, B
ANIMAL PRODUCTION Vol 8, No 3 (2006): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objective of the research was to evaluate the speed and duration of centrifugation and seminal plasma levels on the viability of stallion spermatozoa.   Semen was collected from three stallions twice a week. The collected semen then evaluated macro- and microscopically.  Exp I, the semen was diluted 1:1 with skim milk extender and then centrifuged at 2000 and 3000 rpm for 15 and 20 minutes each. The seminal plasma was removed, and the sperm pellet was re-diluted with skim milk extender. The extended semen then divided in two tubes, stored in 5oC and in a room temperature.  The semen then was examined every 3 hours for room temperature and 12 hours for 5oC.  Exp II, the semen diluted 1:1 with skim milk extender and then centrifuged at 3000 rpm for 15 minutes. The seminal plasma was removed and the pellet was re-diluted with skim milk extender consisted of 0%, 25%, 50% and 75% of the seminal plasma.  The extended semen then was stored in 5oC and examined daily.  Results showed that speed and duration of centrifugation had no effect on the quality of stallion semen. The liquid semen without seminal plasma showed 47.50% motile sperms and 61.00% life sperms, wich were significantly higher (P<0.05) than those with seminal plasma. It was concluded that the speed and duration of centrifugation had no effect on the semen quality and removal of seminal plasma is beneficial for stallion spermatozoa. (Animal Production 8(3): 160-167 (2006) Key Words : Centrifugation, stallion semen and seminal plasma
The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender Yusuf, T Laswardi; Arifiantini, RI; Mulyadi, Y
ANIMAL PRODUCTION Vol 8, No 3 (2006): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The aims of this experiment was to obtain the effects of  different time of glycerolisation on  the quality of frozen ram semen diluted in Tris Egg Yolk (TEY).  Semen from seven sexually mature local rams were collected using artificial vagina weekly, evaluated macroscopically and microscopically.  Good quality semen then diluted with TEY and 6% glycerol added in three different periods.  The first  step was  diluted in room temperature ( treatment 1), the second glycerolisation was at the end of  equilibration (treatment 2) and the third period was several steps of  glycerolisation during  equilibration (treatment 3). Diluted semen was  packed in straws (Minitub, Germany), equilibrated at 5oC for three  hours,  freezed in liquid nitrogen vapor for 10 minutes and then stored in a container of -196 oC liquid nitrogen.  After 24 hours, the semen was thawed at 37oC for 30 second.  The results of showed that there was no significant differences among treatments (P>0.05).  The percentage of post thawing motility was 42.50% (treatment 1), compared to 38.75% (treatment 2), and 30.00% (treatment 3).  In conclusion, the glycerolization  on frozen ram semen can be done any time of the  equilibration without influencing   the sperm motility. (Animal Production 8(3): 168-173 (2006) Key Words: Glycerolisation, equilibration, ram semen
Determination of Equilibration Time of Stallion’ Semen Freezing with Extender Skim Milk Arifiantini, RI; Supriatna, I; Samsurizal, Samsurizal
ANIMAL PRODUCTION Vol 9, No 3 (2007): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objectives of this experiment were to determine the best equilibration time with extender skim milk in order to maintain the quality of frozen stallion semen.  The semen was collected from three 5-8 years old stallions using artificial vagina.  The semen characteristics and quality were evaluated macro- and microscopically and extended with skim milk extender (1:1).   The semen was centrifuged at 3000 rpm for 15 minutes.  Supernatant was removed and the pellet was re-extended with skim milk extender with 5% glycerol.  The extended semen then packed in mini straw (0.5 mL), equilibrated at 5oC  for 1 hr (E1), 2 hrs (E2) and 3 hrs (E3), frozen in the liquid nitrogen (N2) vapor for 15 minutes and then stored in liquid N2 containerfor 24 hrs.  The straw than thawed at 37oC for 30 seconds.   The percentages of sperm motility and viability were observed.  The result of this research showed that there were no significant differences  between  equilibration at 1, 2 and 3 hrs on the percentages of  sperm motility with the average of post thawed motility was   23.60 ± 7.10%.   The equilibration time affected the sperm viability (P<0.05) in all stallions, with the percentages of viable sperm between 38.10 % and 46.50%. (Animal Production 9(2): 145-152 (2007) Key Words: Equilibration, frozen semen, stallion
Occurrence of Sperm Abnormality of Beef Cattle at Several Artificial Insemination Centers in Indonesia Arifiantini, RI; Purwantara, B; Riyadhi, M
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

In the most species studied sperm abnormalities have long been associated with male infertility and sterility. This study evaluated the sperm morphology (normality and abnormality) of beef cattle at several Artificial Insemination centers in Indonesia. Total of 142 bulls were used in this study; an ejaculate from each bull was examined. A drop of semen was placed on 3-4 glass slides, and smears were prepared and air-dried. The smears were stained with carbolfluchsin-eosin (Williams stain). Types of morphological abnormalities were recorded from 500 cells on each sample. It was recorded that 77.46% samples had low primary sperm abnormalities (<5%), while the high level of primary sperm abnormalities (>10%) was found in 5.63% samples.  Pear shaped was the most frequently type of abnormality found on examined samples (2.24 ± 2.94%); while double head was the lowest (0.01 ± 0.04%). (Animal Production 12(1): 44-49 (2010) Key words : sperm abnormality, beef catlle, artificial insemination center
Co-Authors B Purwantara I Supriatna M Riyadhi, M Samsurizal Samsurizal T Laswardi Yusuf, T Laswardi Wilmientje Marlene Mesang Nalley Y Mulyadi, Y