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Sudiastuti, Sudiastuti

Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Author-ID : 694003

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Pengaruh Lama Pemaparan Obat Anti Nyamuk Kertas Berbahan Aktif Transfluthrin Terhadap Struktur Histopatologi Pulmo Mencit (Mus musculus L.) Dewi, Novia Chamala Sari; Sudiastuti, Sudiastuti; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

This research aims to determine the effects of long exposure(hours/day) of paper-based active anti mosquito-Transfluthrinon the level of damage and lung histology of mice. CompletelyRandomized Design (CRD) was used in this study. Twentymales mice, 3 months old, average body weight 20 g were usedand divided into 4 groups, viz: K (control), P1 (exposure to 4hours/day), P2 (exposure 8 hours/day), and P3 (exposure 12hours/day). Histological observation of the level of damage hadbeen done through the cell nucleus and the diameter of thealveoli was measured. Data were analyzed by using ANOVAfor diameter alveoli followed by Duncan's Multiple Range Test(DMRT). The results showed that the there was a significantdifference (P <0.05) in the alveolar diameter (P3). Meanwhile,There was also a change in the form of cell undergoing alveolaredema, pyknosis, karyorhesis and kayolisis. This resultsindicated that the paper-based mosquito repellent activeingredient transfluthrin caused several changes in alveoluscells.

Pengaruh Air Rebusan Biji Alpukat (Persea americana L.) Terhadap Kadar Gula Darah Mencit (Mus musculus Mill.) Diabetesi Yang Diberi Diet Beras Warna Wahyuni, Dwi; Sudiastuti, Sudiastuti; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

This study was undertaken to investigate the effect of the seed avocado cooking water on lowering blood sugar levels in aloxan-diabetic mouse which has diet of black or red rice. The seed avocado cooking water was administered orally and the blood glucose level was measured weekly for 3 week. The research used a design random complete with 24 male then divided into 6 groups to be treated.The hypoglycaemic activity was evaluated by comparing with the initial blood glucoce level. In aloxan-diabetic mouse, the seed avocado cooking water and diet black or red rice showed their influence to decrease blood sugar levels between 42.72% s.d. 51.06%.The variation of combine the composition diet showed no difference in the effect of a decrease in blood sugar levels

PENGARUH REBUSAN DAUN BINAHONG (Anredera cordifolia) TERHADAP KUALITAS SPERMATOZOA MENCIT (Mus musculus L.) DIABETES Meliyana, Yeni; Sudiastuti, Sudiastuti; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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This research aimed to evaluate the effect of decoction of the leaves binahong in improving of sperm quality of diabetic mice induced by alloxan. The quality of mice sperm including number of spermatozoa, motility and morphology of spermatozoa were determined after 30 days. Completed randomized design was use in this research, 30 mice were devided randomly into 5 groups (negative control, positive control, first treatment dose of 29 mg, 57 mg doses of treatment II and 114 mg doses of treatment III). The results of the quality of spermatozoa were presented as mean ± standar error (SE). Analysis of Variances (ANOVA) was used to determined statistical different among the group, followed by Duncan Multiple Range Test (DMRT) with significant level 95%. The results found that there was an increase in blood sugar levels followed by a decline in the number of spermatozoa, motility of spermatozoa (P>0,05) and the increase in abnormal morphology of spermatozoa (P<0,05) in all groups except the negative control after induced. After being given a dose of binahong decoction variation of the results showed that the number and motility of spermatozoa was highest in the group PIII that were given doses of 114 mg a mean number of spermatozoa (21016,00 ± 130,171) and motility of spermatozoa (34,33 ± 0,88). Meanwhile, the lowest morphological abnormal spermatozoa are group PIII with a mean of (79,33 ± 0,88). The conclusion of this study is a decoction of the leaves binahong is able to improve the quality of spermatozoa diabetic mice along with a decoction of the leaves binahong doses in increments.

Efek Pemanis Buatan Sakarin Dan Sari Buah Naga Merah (Hylocereus costaricensiss) Terhadap Histopatologi Hati Dan Ginjal Mencit (Mus musculus L.) Sari, Ela Santi Mulya; Sudiastuti, Sudiastuti; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

This study aims to determine the effects of saccharin and dragon fruit juice on histopatologic of liver and kidney and determine the effective dose of dragon fruit juice to improve the cells of liver and kidney mice (Mus musculus L.) after fed saccharin. The study design used was completely randomized design (CRD). In this study, the independent variables consist of two control and three treated treatment with 5 replications. The histological data of liver and kidneys of mice (Mus musculus L.) presented descriptively,and evaluation of differences in the structure of the liver and kidney mikroanatomi saccharin and dragon fruit (Hylocereus polyrhizus) be compared to normal structure. The results showed that administration of saccharin at a dose of 15mg/kg BB/day to given effect on the histopathology of kidney and liver of mice. Treatment of the red dragon fruit juice at a dose of 200mg/Kg BB/day may improve liver and kidney mikroanatomi structures damaged by the provision of saccharin. From this research, showed that the red dragon fruit juice swetened with saccharin can inhibition effects of on structure microanatomy the liver and kidney

EFEK HIPOGLIKEMIK EKSTRAK ETANOL BATANG KARAMUNTING (MELASTOMA MALABATHRICUM) TERHADAP KADAR GULA DARAH DAN PERUBAHAN STRUKTUR MIKROANATOMI PANKREAS MENCIT (MUS MUSCULUS L.) DIABETESI Wulandari, Anita; Sudiastuti, Sudiastuti; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.087 KB)

This study aims to determine the effect of ethanol extract of stem Karamunting to improve pancreatic microanatomy structure diabetic mice. The study design used was completely randomized design (CRD which is consisting of negative group control, positive control, and the group treated with 100,250 and 500 mg/kg weigth body dose of karamunting stem extract for 28 days. Blood glucose levels in mice then measured using the blood sugar measuring device gluco meter (Nesco Multicheck) Measurements were taken on day 7 after administration of alloxan. On the 28th day, treatment three at a dose of 500 mg / kg / day showed that the pancreatic cells as the cells in the positive control (normal), whereas treatment 1 at a dose of 100 mg / kg / day and treatment 2 doses 250 mg / kg / day is equal to the negative control. The results showed that the sample extraction rod microanatomy Karamunting able to improve the structure of the pancreas of mice at a dose of 500 mg / kg / day.

Co-Authors Anita Wulandari, Anita Dewi, Novia Chamala Sari Dwi Wahyuni Meliyana, Yeni Rudy Agung Nugroho Sari, Ela Santi Mulya Sudrajat Sudrajat

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