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KARAKTERISASI DAN IDENTIFIKASI BAKTERI DARI TAMBA DAGING BABI (Sus sp.) HASIL FERMENTASI SPONTAN Yana, Nova Yanti Dami; Dharma, Bodhi; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.822 KB)

Abstract

This The aims of the study was to determine the bacteria which grown in fermented of the tamba pork (Sus sp.), the Dayak Bulusu traditional food. Descriptive analysis methods was used in the research. Isolation of bacteria were performed at 10-1 to 10- 10 dilution using NaCl solution. The cells mixture were poured and incubated at 37oC for 24 hours. Characterization of bacteria was done in basis of morphology of colony, Gram- staining, and a partial biochemical tests. Three strains of bacteria were isolated i.e. Pseudomonas, Staphylococcus and Klebsiella, respectively.
Pengaruh Lama Pemaparan Obat Anti Nyamuk Kertas Berbahan Aktif Transfluthrin Terhadap Struktur Histopatologi Pulmo Mencit (Mus musculus L.) Dewi, Novia Chamala Sari; Sudiastuti, Sudiastuti; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.674 KB) | DOI: 10.30872/bp.v10i2.133

Abstract

This research aims to determine the effects of long exposure(hours/day) of paper-based active anti mosquito-Transfluthrinon the level of damage and lung histology of mice. CompletelyRandomized Design (CRD) was used in this study. Twentymales mice, 3 months old, average body weight 20 g were usedand divided into 4 groups, viz: K (control), P1 (exposure to 4hours/day), P2 (exposure 8 hours/day), and P3 (exposure 12hours/day). Histological observation of the level of damage hadbeen done through the cell nucleus and the diameter of thealveoli was measured. Data were analyzed by using ANOVAfor diameter alveoli followed by Duncan's Multiple Range Test(DMRT). The results showed that the there was a significantdifference (P <0.05) in the alveolar diameter (P3). Meanwhile,There was also a change in the form of cell undergoing alveolaredema, pyknosis, karyorhesis and kayolisis. This resultsindicated that the paper-based mosquito repellent activeingredient transfluthrin caused several changes in alveoluscells.
PENGARUH REBUSAN DAUN BINAHONG (Anredera cordifolia) TERHADAP KUALITAS SPERMATOZOA MENCIT (Mus musculus L.) DIABETES Meliyana, Yeni; Sudiastuti, Sudiastuti; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

This research aimed to evaluate the effect of decoction of the leaves binahong in improving of sperm quality of diabetic mice induced by alloxan. The quality of mice sperm including number of spermatozoa, motility and morphology of spermatozoa were determined after 30 days. Completed randomized design was use in this research, 30 mice were devided randomly into 5 groups (negative control, positive control, first treatment dose of 29 mg, 57 mg doses of treatment II and 114 mg doses of treatment III). The results of the quality of spermatozoa were presented as mean ± standar error (SE). Analysis of Variances (ANOVA) was used to determined statistical different among the group, followed by Duncan Multiple Range Test (DMRT) with significant level 95%. The results found that there was an increase in blood sugar levels followed by a decline in the number of spermatozoa, motility of spermatozoa (P>0,05) and the increase in abnormal morphology of spermatozoa (P<0,05) in all groups except the negative control after induced. After being given a dose of binahong decoction variation of the results showed that the number and motility of spermatozoa was highest in the group PIII that were given doses of 114 mg a mean number of spermatozoa (21016,00 ± 130,171) and motility of spermatozoa (34,33 ± 0,88). Meanwhile, the lowest morphological abnormal spermatozoa are group PIII with a mean of (79,33 ± 0,88). The conclusion of this study is a decoction of the leaves binahong is able to improve the quality of spermatozoa diabetic mice along with a decoction of the leaves binahong doses in increments.
Pengaruh Air Rebusan Tepung Cacing Tanah (Lumbricus rubellus) Terhadap Titer Antibodi, Jumlah Leukosit Dan Jenis Leukosit Mencit (Mus musculus L.) Yang Diinfeksi Salmonella enterica Serovar Typhi Patimah, Patimah; Kusumawati, Eko; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.757 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui apakah air rebusantepung cacing tanah berpengaruh terhadap titer antibodi serumdarah mencit, serta jumlah leukosit dan jenis leukosit mencityang diinfeksi bakteri S. Typhi. Penelitian ini bersifateksperimental laboratorik dengan Rancangan Acak Lengkap(RAL), yang terdiri dari 5 kelompok perlakuan dengan 5ulangan. Masing-masing kelompok terdiri dari 5 ekor mencit.Kelompok terdiri atas Perlakuan I diberi air rebusan tepungcacing tanah (TCT) dengan konsentrasi 8%; Perlakuan II diberiair rebusan TCT dengan konsentrasi 16%; Perlakuan III diberiair rebusan TCT dengan konsentrasi 32%; Perlakuan IV diberiair rebusan TCT dengan konsentrasi 64% dan kontrol tanpapemberian air rebusan TCT. Hasil penelitian titer antibodiserum darah mencit akut demam tifoid menunjukkan perubahanpositif demam tifoid menjadi negatif demam tifoid pada tiapkelompok mencit P1, P2, P3 dan P4 (air rebusan tepung cacingtanah 8, 16, 32 dan 64%), pada kelompok kontrol tanpa airrebusan tepung cacing tanah mencit tetap mengalami demamtifoid. Rerata pengukuran jumlah leukosit tertinggi terlihat padakelompok P4 dengan konsentrasi 64% yaitu 8,61/mm3 danterendah pada kelompok kontrol yaitu 3.18/mm3. Mencit dikelompok P1 (Pemberian air rebusan tepung cacing tanah 8%)yaitu 3,14/mm3. Mencit di kelompok P2, P3 dan P4 mengalamikenaikan jumlah leukosit. Data analisis statistik, uji One WayAnova didapatkan perbedaan yang signifikan pada jumlahleukosit antara tiap kelompok mencit (P<0,05). Berdasarkandata analisis statistik jenis leukosit yaitu Neutrofil Segmen,Neutrofil Batang, Eosinofil, Limfosit dan Monosit, pada ujinormalitas didapatkan data berdistribusi normal (P>0,05) danhasil uji homogenitas diperoleh data yang homogen (P>0,05),kemudian dilanjutkan pada uji One Way Anova didapatkantidak ada perbedaan yang signifikan pada jenis leukosit antaratiap kelompok mencit (P<0,05).
Sosialisasi Dan Pelatihan Manajemen Keselamatan Dan Kesehatan Kerja Laboratorium Bagi Guru-Guru SMA Dari Kota Samarinda Dan Tenggarong nugroho, Rudy agung; Subagyono, RR Dirgarini Julia; Lepong, Piter; Mandang, Idris
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 2, No 1 (2018)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.37 KB) | DOI: 10.21831/jpmmp.v2i1.16152

Abstract

Merujuk pada Undang-Undang No. 1 tahun 1970 tentang Keselamatan dan Kesehatan Kerja (K3), laboratorium sebagai sarana pendidikan mempunyai resiko sebagai tempat terjadinya gangguan pada K3. Oleh karena itu perlu adanya pengetahuan yang mendalam mengenai Manajemen K3 bagi guru-guru SMA pengelola laboratorium. Program pengabdian pada masyarakat yang berjudul sosialisasi dan pelatihan manajemen K3 laboratorium bagi 27 guru SMA kota Samarinda dan Tenggarong telah berlangsung untuk meningkatkan pengetahuan manajemen K3 di laboratorium. Program dilaksanakan dengan metode 25% teori; 75% praktek Manajemen K3, penilaian resiko, identifikasi bahaya dan penyusunan draft standard operational procedure (SOP) K3, Cardiopulmonary resuscitation (CPR), Pertolongan pertama pada kecelakaan (P3K) dan bahaya psikososial bertempat di FMIPA Universitas Mulawarman. Hasil yang diperoleh (berdasarkan angket evaluasi) adalah 97% peserta menyatakan peningkatan pengetahuan tentang manajemen K3, penilaian resiko serta identifikasi bahaya, SOP K3, CPR, P3K dan adanya bahaya psikososial di lingkungan laboratorium SMA.
The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured Nugroho, Rudy Agung; Manurung, Hetty; Saraswati, Dewi; Ladyescha, Deasy; Nur, Firman Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6519

Abstract

This research aimed to determine the phytochemicals content of Terminalia catappa leaves extract (TCL) and its effects on the survival and blood profiles of ornamental fish (Betta sp) Ninety fish were randomly assigned into six triplicates groups and reared in various concentration of TCL: 0 (control), 125, 250, 375, 500, 625 ppm for 30 days. Temperature, Dissolve oxygen (DO), and pH were monitored during the trial. After 30 days, survival, Red Blood Cells (RBC), White Blood Cells (WBC), haemoglobin (Hb), lymphocyte, and total protein serum (TPS) were analyzed. Based on the phytochemicals test, saponin, triterpenoid, quinon, phenolic, tannin, and flavonoid were detected on the TCL. Temperature and DO were not affected by any concentration of TCL. The lowest pH (5.05) was found in fish medium immersed with 625 ppm of TCL. Adding TCL above 375 ppm resulted in significantly higher survival, RBC, and Hb. The highest WBC was found in fish immersed with 625 ppm whereas the lowest lymphocyte was found in fish immersed with 375 of TCL. However, immersing any various concentration of TCL did not affect on the TPS. In summary, immersing TCL above 375 ppm is beneficial to enhance survival, RBC, WBC, and Hb of Betta sp.How to CiteNugroho, R. A., Manurung, H., Saraswati, D., Ladyescha, D. & Nur, F. M. (2016). The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured. Biosaintifika: Journal of Biology & Biology Education, 8(2), 240-247.
Growth and Survival Evaluation of Oreochromis Sp fed Hermetia illucens Larva and Manihot esculenta leaves Meal Aini, Nur; Nugroho, Rudy Agung; Hariani, Nova
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.587 KB) | DOI: 10.15294/biosaintifika.v10i3.15278

Abstract

This study was conducted to compare the effects of dietary substitution of fish meal (FM) with black soldier fly (Hermetia illucens) larvae meal (BSFM) and Manihot esculenta leaves meal (MEM) on the growth and feed efficiency of Oreochromis sp. Four concentrations viz: P1 (25%): 50g BSFM and 25g MEM, P2 (50%): 100g BSFM and 50g MEM, P3 (75%): 150g BSFM and 75g MEM, P4 (100%): 200g BSFM and 100g MEM were prepared and tested against control without FM replacement. Each diet was fed to three replicates groups of fish at a rate of 5% of body weight two times per day for 30 days. At the end of the trial, growth parameters, Feed conversion rate (FCR), and feed efficiency (FE) were evaluated. The results showed that fish fed dietary substitution of FM with combination ratio of BSFM and MEM higher than 50% significantly improved all growth parameters, FCR and FE. It is therefore suggested that the partial (higher than 50%) or total replacement of fish meal with combination of BSFM and MEM in the diet of Oreochromis sp can be used as fish meal substitution to obtain better growth and feed efficiency.
Pengaruh Rasio Pupuk Organik Cair Limbah Ikan Nila (Oreochromis niloticus) Dengan Pupuk Inorganik Komersial Terhadap Pertumbuhan Sawi Pakcoy (Brassica rapa L.) Secara Hidroponik Rakit Apung Priyanggi, Rizki Wira; Nugroho, Rudy Agung; Sari, Yanti Puspita
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 14 No 1 (2019): Bioprospek: Jurnal Ilmiah Biologi: Volume 14 Number 1 2019
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.352 KB) | DOI: 10.30872/bp.v14i1.429

Abstract

Studi pengaruh Rasio Pupuk Organik Cair Limbah Ikan Nila (Oreochromis niloticus) dengan Pupuk Inorganik Komersial terhadap Pertumbuhan Sawi Pakcoy (Brassica rapa L.) secara Hidroponik Rakit Apung dilaksanakan pada bulan September 2018 sampai Januari 2019. Penelitian ini bertujuan untuk mengetahui pengaruh rasio pemberian pupuk organik cair limbah ikan nila terhadap pertumbuhan tanaman sawi pakcoy secara hidroponik rakit apung, untuk mengetahui konsentrasi pupuk organik cair limbah ikan nila terbaik untuk pertumbuhan tanaman sawi pakcoy yang optimal pada. Penelitian ini menggunakan rancangan acak kelompok (RAK) yang terdiri dari 5 perlakuan (100% AB mix, 25% POC dan 75% AB mix, 50% POC dan 50% AB mix, 75% POC dan 25% AB mix, 100% POC) dengan 4 ulangan. Pengamatan dilakukan selama 4 minggu. Parameter lingkungan yang diamati adalah suhu lingkungan (rumah hidroponik), suhu larutan, pH larutan dan TDS (Total Dissolved Solid). Parameter pertumbuhan yang diamati yaitu, tinggi tanaman, jumlah daun, berat segar, berat kering, luas daun, kandungan klorofil, laju pertumbuhan relatif tanaman, dan volume akar. Hasil penelitian ini menunjukkan perlakuan dengan menggunakan 100% AB mix dan perlakuan 25% POC dan 75% AB mix merupakan perlakuan terbaik pada parameter tinggi tanaman, jumlah daun, luas daun, berat segar, berat kering, volume akar, laju pertumbuhan relatif, dan kandungan klorofil. Pada penelitian ini dapat disimpulkan bahwa substitusi lebih dari 25% POC belum mampu memberikan pertumbuhan yang optimum seperti pupuk AB mix.
Pengaruh Susu Fermentasi Lactobacillus Casei Terhadap Hematologi Mencit (Mus musculus) Yang Diinfeksi Salmonella enterica Serotype Typhi Nurlilayanti, Nurlilayanti; Kusumawati, Eko; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 14 No 1 (2019): Bioprospek: Jurnal Ilmiah Biologi: Volume 14 Number 1 2019
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.594 KB) | DOI: 10.30872/bp.v14i1.430

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh susu fermentasi Lactobacillus casei terhadap hematologi mencit yang diinfeksi Salmonella enterica serovar typhi (S. typhi). Tahapan penelitian ini yaitu pemeliharaan hewan uji, pembuatan media, perlakuan bakteri S. typhi pada mencit, pemeriksaan demam tifoid (uji widal), analisis hematologi mencit dan analisis data. Dalam penelitian ini, sekelompok mencit dibagi menjadi empat kelompok, yaitu K1 merupakan mencit kelompok kontrol negatif, K2 merupakan mencit kelompok kontrol positif, P1 mencit yang diberi perlakuan S. typhi inkubasi 6 jam (0,25 mL) dan susu fermentasi (0,25 mL) secara bersama-sama selama 7 hari dan P2 mencit diberi perlakuan S. typhi inkubasi 6 jam (0,25 mL) selama 7 hari dilanjutkan dengan pemberian susu fermentasi (0,25 mL) selama 5 hari. Hasil analisis uji statistik menyebutkan bahwa pemberian susu fermentasi pada mencit perlakuan P2, nilai hemoglobin (Hb) sebesar 4,20±0,20 dan hematokrit (Hct) sebesar 15,85±3,35, sedangkan pada P1 (mencit diinfeksi S. typhi dan pemberian susu fermentasi secara bersama-sama) tidak dipengaruhi, hal ini dikarenakan efek antagonis dari bakteri Lactobacillus terhadap bakteri Salmonella. Kesimpulan dari penelitan ini adalah bahwa mencit yang diberi perlakuan S. typhi dan susu fermentasi selama 7 hari tidak menunjukkan pengaruh terhadap hematologi mencit. Sedangkan pada mencit yang diberi perlakuan S. typhi selama 7 hari dilanjutkan dengan pemberian susu fermentasi selama 5 hari, berpengaruh terhadap hematologi mencit pada parameter Hb dan Hct.
Hubungan Panjang Berat Dan Faktor Kondisi Relatif Lima Spesies Ikan di Sungai Suwi Muara Ancalong, Kutai Timur Nugroho, Rudy Agung; Florentino, Apri Pauci; Lariman, Lariman; Aryani, Retno; Rudianto, Rudianto; Kusneti, Monica
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 2 (2021): June 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i2.3524

Abstract

The length-weight relationship of fish and the condition factor provides an overview of the fish growth conditions in the river. The purpose of this study was to determine the length-weight relationship and factor condition of five fish species in the Suwi River wetlands. The method of catching fish used gill nets, ceiling nets, fishing rods, traps, and other nets. The research was conducted for to months in the Suwi river wetlands. The results the length-weight relationship of five fish were varied, Ompok bimaculatus had b value 2,93, Helostoma temminckii (b  value 2.15), Mystus nemurus  (b value 2,62), and Channa) micropeltes (b 2.61) which indicated a negative allometric growth pattern. Meanwhile Leptobarbus hoevenii (b 3.12) was a positive allometric growth pattern. Negative allometric indicates growth in length faster than weight growth, thus the physical shape looks flattened, while positive allometric indicates growth in fish length slower than growth in fish weight. Based on the relative weight (Wr) of Ompok bimaculatus 101.28 ± 19.13, Helostoma temminckii 101.05 ± 15.51, Mystus nemurus 100.95 ± 14.84, Channa micropeltes 101.09 ± 14.14, Leptobarbus hoevenii 103.71 ± 34.57. Condition factor above 100, indicates sufficient food availability or low predator density
Co-Authors Abd. Rasyid Syamsuri Andi Asikin Noor Andi Noor Asikin Andy Nurcahyono Anggi Audila Anton Rahmadi Apri Pauci Florentino Aprilya Mustari Ari Susandi Sanjaya Ari Susandy Sanjaya Ari Susandy Sanjaya Aryani, Retno Auliana Auliana Ayu Nirmala Sari Bambang Purnama Blegur Megaria Ismawati Deasy Ladyescha, Deasy Dewi Saraswati, Dewi Dewi, Novia Chamala Sari Dharma, Bodhi Didit Suprihanto, Didit Dwikie Setiawan Dyah Noprianti Eko Kusumawati Eno Framita Fatmawati Patang Firman M Nur Firman Muhammad Nur, Firman Muhammad Florentino, Apri Pauci Hadi Purwo Wiyono Hendra Saputra Hetty Manurung Hetty Manurung Hetty Manurung Hetty Manurung Hetty Manurung Hetty Manurung Hetty Manurung, Hetty Idris Mandang, Idris Idwan Jul Ulum Ilham Zulfahmi Ilmania Laora Sunan Tias Imam Rosadi Ingga Lies Dastin Islamiati Islamiati Iwan Hasri Iwan Muhammad Ramdan Jawatir Pardosi Julinda Romauli Manullang Kusneti, Monica Kusumawati, Eko Kusumawati, Eko Lariman Lariman Lariman Lariman Lariman, Lariman Lepong, Piter Mardi Irwanto Meliyana, Yeni Monica Kusneti Muhammad Erwhyn Salim Nasution, Annio Indah Lestari Normala Sari Nova Hariani Nuraeni Muzdalifah Nurlilayanti, Nurlilayanti Panggabean Panggabean Patimah Patimah Prima Hidayat Priyanggi, Rizki Wira Rahma Suci Retno Aryani Retno Aryani Retno Aryani Retno Aryani Retno Aryani Retno Aryani. RR Dirgarini Julia Nurlianti Subagyono Rudianta Rudianta Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Rudianto Saibun Sitorus Subagyono, RR Dirgarini Julia Sudiastuti Sudiastuti Sudiastuti, Sudiastuti Syafrizal Fachmy Veliyana Londong Allo Wardina Masdalifa Widha Prahastika Widha Prahastika Widha Prahastika Widha Prahastika Windi Rosiana Dewi Wulan Iyhig Ratna Sari Yana, Nova Yanti Dami Yanti Puspita Sari Yanti Puspita Sari Yerwanto Ilang Yudha Aulia Firnanda