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Journal : EKSAKTA: Journal of Sciences and Data Analysis

Solvent Optimization For Genistein Isolation Of “Rotten Tempe” By High Performance Liquid Chromatography Method Cucun Alep Riyanto; Hartati Soetjipto
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 17, ISSUE 2, August 2017
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol17.iss2.art3

Abstract

Genistein is a soy isoflavone that has been known have anticancer properties. The aim of this research is to determine the right combination of solvents to extract isoflavones in rotten tempe and determine the genistein content of the extraction process by the High Performance Liquid Chromatography (HPLC) method. Isoflavone extraction process was done using a mixture of methanol and chloroform solutions. Isoflavone extracts obtained will be analysed using High Performance Liquid Chromatography (HPLC) method to determine the content of genistein. The optimum isoflavone extract was obtained from chloroform:methanol (10:1 v/v) solvent ratio. Where as the highest contents of genistein from the 4th day “rotten tempe” as 26.199 ± 25.146 (mg/g).
Protoporphyrin IX Extraction from Quail Eggshell (Cortunix cortunix) and Its Complexity with Zn(II) A. Rindang Anggit Laksono; Yohanes Martono; Cucun Alep Riyanto
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 18, ISSUE 2, August 2018
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol18.iss2.art7

Abstract

The aim of this research is to find the optimal condition for  Zn(II) complexation with protoporphyrin IX which is a natural coloring pigment on quail eggshell. The concentration of protoporphyrin IX extract is  0.01% (w / w) of the dried extract. The results of the complex are analyzed using Response Surface Methodology (RSM). The design used was central composite design with 3 factor variables X1 = pH (2; 4; 6), X2 = ratios (1: 3,1: 4,1: 5) and X3 = time (30 ', 60', 90 '). The modeling used is linear and shows that the pH, ratio, and time factor have an influence on the complexation. This model gives the absorbance equation Y = 2.12506 - 0.049856X1 - 020316X2 - 0,00409857X3 and gives treatment in the most optimum complexation of pH 2; ratio of 1: 3; and time for 30 minutes with the absorbance of 1,293 and the desirability value of 0.825.
Synthesis and Characterization of Nano Activated Carbon from Annatto Peels (Bixa orellana L.) Viewed from Temperature Activation and Impregnation Ratio of H3PO4 Cucun Alep Riyanto; Muhamad Syaiful Ampri; Yohanes Martono
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 1, ISSUE 1, February 2020
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol1.iss1.art7

Abstract

Nano activated carbon is activated carbon with nano-sized carbon particles and can be synthesized from cellulose-containing materials such as the Annatto peels. In this study, the synthesis of nano activated carbon of the Annatto peels was carried out in terms of activation temperature variations and the carbon impregnation ratio of 50% H3PO4 acid and determining the characteristics of nano activated carbon from Annatto peels (NAPAC). The activation method used is the impregnation of carbon in 50% H3PO4 with a ratio of 1: 3; 1: 4; 1: 5; 1: 6; and 1: 7 (w/w) for 24 hours and heating at 400; 500; 600; 700; and 800 °C for one hour. Nano activated carbon from Annatto peels (NAPAC) was characterized by Infrared Spectrophotometer (FTIR), X-ray Diffractometer (XRD), and Transmission Electron Microscope (TEM). The results of the study showed that the NAPAC can be synthesized from Annatto peels with activation by 50% H3PO4 at the temperature of 500°C and the impregnation ratio of 1:5 (w/w). The result of characterization using FT-IR, XRD and TEM showed that NAPAC contains a group of functions O-H, C-H, C=, C=C dan C-O/P=O with an amorphous carbon structure and the range of particles diameter at 22-36 nm