Article Per Year (5 Year)
p-Index From 2019 - 2024
0.659
P-Index
This Author published in this journals
All Journal JURNAL ILMIAH PLATAX
Pratasik, Silvester Benny
Unknown Affiliation
Environmental Science

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Antibacterial Activity of Endophytic Bacteria of Seagrass Symbiont Enhalus acoroides from Tiwoho Waters, North Minahasa Purniasih, Ni Komang Pitri; Ginting, Elvy Like; Wullur, Stenly; Mangindaan, Remy E. P.; Rumampuk, Natalie D. C.; Pratasik, Silvester Benny
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42485

Abstract

Nutrient-poor marine bacteria are often found to form a living mechanism by associating with other marine organisms such as seagrass Enhalus acoroides. Seagrass serves as a habitat for marine biota and is known to produce bioactive compounds. Endophytic and epiphytic microbes that are symbiotic in seagrass have the ability to produce bioactive compounds similar to the bioactive compounds produced by their host. Therefore, this study aimed to obtain isolates of symbiont bacteria and to test their antibacterial activity.  The symbiotic bacteria isolates were tested for their antibacterial activity using gram-negative bacteria such as E. coli and S. typhi, and gram-positive bacteria such as S. aureus and S. mutans. Antibacterial activity test was carried out using the paper disc method. Observation of antibacterial activity was carried out for 3x24 hours by observing the growth of the resulting inhibition zone. The zone of inhibition was measured to determine the diameter and strength of the bioactive compounds produced by symbiotic bacteria.A total of six isolates of symbiotic bacteria were obtained from the seagrass Enhalus acoroides with varied morphological characteristics. The six isolates of symbiont bacteria showed antibacterial activity and could inhibit and kill pathogenic bacteria. Two of the six isolates of symbiotic bacteria that produced the largest diameter of the inhibition zone were isolates E (Ep1) 3,5 mm and F (En3) 4 mm in S. mutans test bacteria.Keywords: Antibacterial, Isolation, Enhalus acoroides, Symbionts, Tiwoho AbstrakBakteri laut yang miskin nutrisi banyak dijumpai membentuk mekanisme hidup dengan cara berasosiasi dengan organisme laut lainnya seperti lamun Enhalus acoroides. Lamun berfungsi sebagai habitat biota laut dan diketahui dapat menghasilkan senyawa bioaktif. Mikroba endofit dan epifit yang bersimbiosis pada lamun mempunyai kemampuan untuk memproduksi senyawa-senyawa bioaktif yang serupa dengan senyawa bioaktif yang diproduksi inangnya. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri simbion serta menguji aktivitas antibakterinya.  Isolat bakteri simbion diuji aktivitas antibakterinya menggunakan bakteri uji gram negatif seperti E. coli dan S. typhi, dan bakteri uji gram positif seperti S. aureus dan S. mutans. Uji aktivitas antibakteri dilakukan menggunakan metode kertas cakram. Pengamatan aktivitas antibakteri dilakukan selama 3x24 jam dengan mengamati pertumbuhan zona hambat yang dihasilkan. Zona hambat diukur untuk mengetahui diameter dan kekuatan senyawa bioaktif yang dihasilkan oleh bakteri simbion.  Sebanyak enam isolat bakteri simbion yang didapatkan dari lamun Enhalus acoroides dengan karakteristik morfologi yang bevariasi. Keenam isolat bakteri simbion menunjukkan adanya aktivitas antibakteri dan dapat menghambat serta membunuh bakteri pathogen. Dua dari enam isolat bakteri simbion menghasilkan diameter zona hambat terbesar adalah isolat E (Ep1) 3,5 mm dan F (En3) 4 mm pada pada bakteri uji S. mutans.Kata kunci: Antibakteri, Isolasi, Enhalus acoroides, Simbion, Tiwoho
A Preliminary Studies of Fish Richness in the Tondano River Estuary Manado Bay Bataragoa, Nego E.; Pratasik, Silvester Benny; Menajang, Febry S. I.; Manginsela, Fransine B.; Dauhan, Dulce Maria; Tombi, Indra
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.50025

Abstract

The purpose of this study was to analyze the richness of fish species in the Tondano River estuary. Sampling was done for two months, in July and August 2022, new moon phase, during the low tide at 13.00-16.00 pm. Sampling used a 20-m beach seine with a cod-end part of 0.2-cm mesh and wings of 2-m height and 1.5-cm mesh. The beach seine was operated 10 times starting from the lowest tide at 13.00 pm to the tide condition at 16.00 pm. As a whole, 736 individuals were caught, consisting of 24 species and 17 families, 14 migrant species, and 10 resident species The number of fish varied from 1 to 445 individuals, in which Terapon jarbua (445 ind) was the most abundant, followed by Neovespicula depressifrons (70 ind). The dominant index (C) was 0.38 and the diversity index (H) was 1.65. The resident species were 120 individuals varying from 2 to 70 ind., and the most abundant was Neovespicula depressifrons (70 ind.). The dominant index (C) was 0.37 and the diversity index was 1.47.
Fish Community Structure Of The Bahu River Estuary Manado City: Sampe, Ayumi Angraini; Manu, Gaspar D.; Kambey, Alex D.; Pratasik, Silvester Benny; Rondonuwu, Arie B.; Rangan, Jety K.
Jurnal Ilmiah Platax Vol. 12 No. 2 (2024): ISSUE JULY-DECEMBER 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i2.55275

Abstract

Community structure is an ecological term to indicate what organisms are present in a given environment, in what numbers, and how they are interconnected, through ecological indices that reflect dominance, evenness, and richness traits. The estuary is the most downstream part of the river and is connected to the sea. The Bahu River estuary is a habitat for a variety of aquatic life, especially fish, where water quality conditions greatly affect the abundance, diversity, and dominance. The methods used in this study were general field survey methods and fishing methods using the "swept area" method (Sparre & Venema, 1998). Data collection was carried out based on the time of collection using beach seine gear. This research was conducted with several stages, namely fishing in the field which will become data, data analysis and discussion. The results of this study indicate the composition of fish species in the Bahu River Estuary there are 11 families, 11 genus, and 14 species with a total of 86 individuals. The results of data analysis of the highest relative abundance in the Bahu River Estuary were in June, namely the Ambassis urotaenia species with a value of 45.90%. The results showed the community structure in the Bahu River Estuary in June and July (H' = 1.53 - 1.64, J' = 0.64 - 0.79, D = 0.31 - 0.26). Keywords: Diversity; Evenness; Dominance; River Estuary.
Co-Authors Alex D. Kambey Dauhan, Dulce Maria Fransine B. Manginsela Gaspar D. Manu, Gaspar D. Ginting, Elvy Like Menajang, Febry S. I. Natalie D Rumampuk Nego E. Bataragoa, Nego E. Purniasih, Ni Komang Pitri Rangan, Jety K. Remy E. P Mangindaan Rondonuwu, Arie B. Sampe, Ayumi Angraini Stenly Wullur Tombi, Indra