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Detection of Foot and Mouth Disease Virus in Cattle in Lamongan and Surabaya, Indonesia Using RT-PCR Method Dinana, Zayyin; Rantam, Fedik Abdul; Suwarno, Suwarno; Mustofa, Imam; Rahmahani, Jola; Kusnoto, Kusnoto
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.191-196

Abstract

Foot and mouth disease (FMD) virus was reported as an outbreak in Indonesia in April 2022 and belonged to serotype O/ME-SA/Ind-2001e is spread in the country. This study aimed to detect the causative agent based on clinical symptoms in cattle that have the vesicle in the mouth and hooves. A total of 25 samples were collected during August 2022 from Lamongan and Surabaya, Indonesia. FMD was identified in 58% (7/12) using reverse transcription polymerase chain reaction (RT-PCR), respectively. The samples were performed using universal primers with 328 bp length for primary diagnosis of FMD. These findings indicate that the spread of FMD viruses is highly contagious, so rapid and accurate diagnosis is needed as an effort to control and monitor FMD viruses.
EPIDEMIOLOGY OF ROTAVIRUS INFECTION IN CHILDREN UNDER FIVE YEARS IN DR. SOETOMO HOSPITAL, SURABAYA (2015-2019): Gambaran Epidemiologi Kasus Infeksi Rotavirus Pada Balita di RSUD Dr. Soetomo Pada Tahun 2015-2019 Ana Bilah, Aisah Nur; Dinana, Zayyin; Maharani, Aussie Tahta; Fitria, Anisa Lailatul; Juniastuti; Athiyyah, Alpha Fardah; Darma, Andy; Gunadi Ranuh, I Gusti Made Reza; Sudarmo, Subijanto Marto; Yamani, Laura Navika
Jurnal Berkala Epidemiologi Vol. 12 No. 3 (2024): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V12I32024.255-262

Abstract

Background: Diarrhea is the first cause of death for children under five years of age worldwide. In Indonesia, diarrhea cases due to rotavirus infection tend to be detected throughout the year. Purpose: The study was to determine the epidemiology of diarrhea cases due to rotavirus infection of pediatric patients in Dr. Soetomo Hospital, Surabaya based on the variables of person, place, and time. Methods: This type of research uses both descriptive and quantitative methods. The samples used were cases of rotavirus infection in toddlers in Dr. Soetomo Hospital, Surabaya in 2015–2019. Results: From 2015 to 2019, there were about 110 cases (31.98%) of acute diarrhea caused by rotavirus infection at Dr. Soetomo Hospital, Surabaya. The findings revealed that the majority of rotavirus infections (42.73%) occurred in children aged 6-11 months followed 12-23 months (35.45%) and were male (56.36%). The highest cumulative incidence was observed in August 2018 and February 2029, with a value of 1,0. Rotavirus infection was most common among children living in Gubeng sub-District, with 19 cases (17.27%). Conclusion: Epidemiological studies of rotavirus infection by person, place, and time reveal the distribution of disease cases and enable targeted interventions to respond to the disease.