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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Indonesian Journal of Human Nutrition JURNAL PHOTON Oto Rhino Laryngologica Indonesiana BKM Public Health and Community Medicine
Surono, Agus
Departemen Ilmu Kesehatan Telinga Hidung Tenggorok- Bedah Kepala Leher Fakultas Kedokteran Universitas Gadjah Mada/ Rumah Sakit Dr. Sardjito Yogyakarta
Agriculture, Biological Sciences & Forestry Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Journal : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Expression of Tumor Necrosis Factor - α (TNF-α) and Interleukin 1-β (IL1-β) in Chronic Tubotympanic Suppurative Otitis Media Anton Budhi Darmawan; Marsetyawan HNE Soesatyo; Ratna Dwi Restuti; Agus Surono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.283 KB) | DOI: 10.19106/JMedScieSup0050012018020

Abstract

Chronic Suppurative Otitis Media (CSOM) is a common public health problem worldwide and a major cause of hearing impairment. It is also one of the neglected disease especially in developing countries. Cytokines are a group of glycoproteins that play a role in strengthening the immune and inflammatory reactions in various diseases, including inflammation of the middle ear. Some of the important inflammatory mediators found in middle ear fluids are Tumor Necrosis Factor-α (TNF-α) and Interleukin-1β (IL-1β). Cytokines are thought to play a role in the ongoing inflammatory regulation. The aim of this study was to compare the expression of TNF-α and IL-1β in tubotympanic CSOM and in healthy control group. The mean of TNF-α serum level in tubotympanic CSOM was 0,553±1,59 pg/ ml, and 0,587±2,13 pg/ ml in control group. There was no statistically different of TNF-α between two groups (P > 0,05). Mean of IL-1β serum level in the tubotympanic CSOM and control group were 0,633±0,92 and 0,302±0,48, respectively. Although IL-1β levels were higher in the patient group, the difference was not statistically significant (P > 0,05).
Sequence variation of latent membrane protein 2A (LMP2A)gene from Epstein-Barr virus epitope cytotoxic T-lymphocyte (CTL)related to human leucocyte antigen-A24 (HLA-A24)in peripheral blood sample and cytobrushof nasopharyngeal cancer patients Maya Esther Wullur Moningka; Agus Surono; Sofia Mubarika
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 2 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.639 KB) | DOI: 10.19106/JMedSci005102201905

Abstract

Epstein-Barr virus (EBV) infects lymphocyte B and triggers latent phase in the host so that it causes nasopharyngeal carcinoma (NPC). Latent membraneprotein 2A (LMP2A) epitope CTL-HLA-A24 is a target for recognition by cytotoxic T lymphocyte(CTL). The change in the epitope could influence the latency of particular EBV in the host due toits ability to evade immune surveillance mediated by CTL. The study aimed to determine thesequence variation of LMP2A epitope CTL-HLA-A24 gene from the peripheral blood samples and cytobrush of the NPC patients. Case-series study was conducted with total 16 cytobrush samples from NPC patients. DNA isolation, polymerasechain reaction (PCR) and gene sequencing were performed in this study. From cytobrush samples of NPC patients, it was found the changes of base sequence variation of LMP2A gene from GGC>GGA, CCA>CCC, TGC>TCC, GGT>GGC and TCT>ACT. CCA>CCC and TGC>TCC variations were found in epitope associated with HLA-A2 where there was a change of epitope sequence from TYGPVFMCL to TYGPVFMSL caused by missense mutation. The change of base sequence caused amino acid alteration from cysteine to serine. Whereas the variation of CCA>CCC did not change the sequence of amino acid proline so that the epitopewas unaffected. In epitope associated HLA-A2 (CLGGLLTMV), there was a change in base sequence from GGT to GGC, but there was no changes in amino acid and still as glycine. There were some new variations: in the upstream sequence of LMP2A from GGC>GGA which is silent mutation and the other variation is in downstream sequence of LMP2A from TCT>ACT which is missense mutation. Thesequence variations of LMP2A gene found in this research were GGC>GGA, CCA>CCC, TGC>TCC, GGT>GGC and TCT>ACT. In our research, we found another variation compared the previous research. The variation was in the upstream sequence of LMP2A from GGC>GGA which is silent mutation and the other variation is in the downstream sequence of LMP2A from TCT>ACT which is missense mutation.
Co-Authors Adi Heru Sutomo Adi Heru Sutomo Ahmad Imanuddin Angelina Candra Dewi Anton Budhi Darmawan Aprianti, Rina Asrul Sani Azwar Azwar Fuadiyah Nila Kurniasari Ika Ambar Trisnawati Koeswardani, Millani Amastasia Lia Agustina, Lia Marsetyawan HNE Soesatyo Maya Esther Wullur Moningka Nur Arfian, Nur Rahmani, Noor Siti Ratna Dwi Restuti Retno Pangastuti Rina Aprianti Sofia Mubarika Sofia Mubarika Tunika, Puriwati Wijaya, Muhammad Harry