Molekul: Jurnal Ilmiah Kimia
Vol 10, No 2 (2015)

THE CHARACTERISTICS OF BIOACTIVE PEPTIDES AND ANTIBACTERIAL ACTIVITY OF HONEY BEE (Apis nigrocincta) SMITH VENOM, ENDEMIC TO SULAWESI

Mokosuli Yermia Semuel (Laboratory of Bioactivity and Molecular Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Manado, Manado, North Sulawesi)
Rudi Alexander Repi (Research Institutes, State University of Manado)

Article Info

Publish Date
01 Nov 2015

Abstract

Apis nigrocincta Smith is a species of honey bee cavity nesting, endemic to Sulawesi. Research that aims to find the composition of the bioactive content of peptides and antibacterial activity of honey bee venom A. nigrocincta Smith has been conducted. Honey bee venom composition was analyzed using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Method and Spectrophotometer UV-Vis Method. Analysis of antibacterial activity, was conducted using a modified agar diffusion method. The results showed that the venom of the honey bee Apis nigrocinctaSmith has five bands of molecules with a molecular weight i.e. 33.54kDa; 21 kDa and 15.43 kDa. The peptide detected were hyaluronidase, fosfolipase A, mellitin, lysofosfolipase or antigen 5. Antibacterial activity was higher than the control ampisilin and antibiotic streptomycin.

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Journal Info
Molekul: Jurnal Ilmiah Kimia
Website

Abbrev

jm

Publisher

Universitas Jenderal Soedirman

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

MOLEKUL is a peer-reviewed journal of chemistry published by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Indonesia. Publishing frequency 2 issues per year, on May and November. This Journal encompasses all branches of chemistry and its ...