The research aimed to know the best formulations of basic substrate inoculums white oyster mushrooms (Pleurotus ostreatus (Jacq) P. Kumm) in the best liquid forms and their viability on production media. The research was conducted at Laboratory Biotekchnology of Depertement of Biology Faculty of Matematics and Natural Sciences, starting from March until July 2015. The experimental designed used was a Completely Randomized Design (CRD) which consisted of seven treatment and three replications. The compositions of the treatment were the ratio among wood sawdust, rice brain, corn starch and calcium (g) in successive sequences as follows : P0 (0 : 50: 50 : 3); (P1 10 : 45 : 45 : 3); (P2 20 : 40 : 40 : 3); (P3 30 : 35 : 35 : 3); (P4 40 : 30 : 30 : 3); (P5 50 : 25 : 25 : 3); (P6 60 : 20 : 20 : 3). The planting media used in this research were production media. The observation parameters namely the ability fungal growth of inoculums that meets surface the media with viability a high. The result of the research showed that P6 was the best treatment among. In the viability of the inoculums grew well on media production surface with good viability at 8 days of incubation.
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