International Journal of Biosciences and Biotechnology
Vol 5 No 2 (2018)

DETECTION AND ELIMINATION VIRUS ON POTATO (Solanum tuberosum L.)

Debora Margareth (Program of Master of Biological Sciences, Faculty of Mathematics and Natural Sciences, Udayana University)
Ida Ayu Astarini (Program of Master of Biotechnology, Postgraduate Program of Udayana University)
I Gede Rai Maya Temaja (Program of Master of Biotechnology, Postgraduate Program of Udayana University)



Article Info

Publish Date
21 May 2018

Abstract

This study aims to determine the symptoms of virus attacks on potato crops, identification of viruses that attack potato plants in Pancasari village, Bali, efectivity of thermotherapy technique to eliminate in vivo potato. The research was taken using purposive sampling technique and complete random program. Virus symptoms were observed on two potato fields. Serological test employed DAS-ELISA with PVX, PVS, PVY and PLRV antiserum. In vivo elimination virus were done by thermoteraphy in 37°C±1°C (4 days), 34°C±1°C (3 days) on 3 weeks and 33°C±1°C (4 days), 30°C±1°C (3 days) on 2 weeks. Results of the study at Pancasari Village Bali shown varied symptoms on potato leaves, such as mottle, mosaic, malformation, leafroll, witchesbroom. Through serological test, had identified viruses were 11.11% PVX, 55.56% PVS, 80% PVY and 11.11% PLRV which were infected farmer’s potato. In vivo thermotherapy was succeed decreasing PVS and PVY level during 2 weeks on 33°C±1°C (4 days) and 30°C±1°C (3 days).

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Journal Info

Abbrev

jbb

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Environmental Science

Description

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of ...