ABSTRACT---One of the Indonesian marine natural resources, brown alga of Turbinaria decurrens, used in pharmacy in order of carotenoid pigment as antioxidant. We interested in analysis B-caroten in Turbinaria decurrens and antioxidant and anti-tumor activity. The method that is use to divide carotenoid is the hierarchy maseration method using n-hexane, ethyl acetate and methanol and then using chromatography column. To analyze the qualitative carotenoid uses HPLC by Crc column and eluenmethanol-acetonitrile (3:I). Meanwhile, the test of bioactivity carotene uses radical DPPH (1I'difenil-2-pibilhidrozil) to test of antioxidant qnd sitotoksik asscy with MTT [3,(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrcaolium bromideJ for HeLa tumor cells to test of anti-tumor. The result of this research is B -caroten thqt is in qctract has 0.00387%. Moreover, bioacttvity test shows that B -caroten fraction does notactive to neutralize of DPPHfree radical than ascorbic aci{ but it shows the acttvity to kill HeLa tumor cells.Keywords : Turblnaria decunens, Carolenoid, Antioksidan, Antitumor
Copyrights © 2009