journal of Basic Medical Veterinary
Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019

IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG

Akyun Rozaqi Syah Putra (Fakultas Kedokteran Hewan Universitas Airlangga)
Mustofa Helmi Effendi (Fakultas Kedokteran Hewan Universitas Airlangga)
Setiawan Koesdarto (Fakultas Kedokteran Hewan Universitas Airlangga)
Suwarno Suwarno (Fakultas Kedokteran Hewan Universitas Airlangga)
Wiwik Tyasningsih (Fakultas Kedokteran Hewan Universitas Airlangga)
Agnes Theresia Soelih Estoepangestie (Fakultas Kedokteran Hewan Universitas Airlangga)

Article Info

Publish Date
02 Jul 2020

Abstract

Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the study showed that from all of the samples could be isolated and indentified E. coli, based on the colony characteristics on EMBA and biochemical test. Based on the double disc synergy test method using antibiotic disc amoxicylyn-clavulanate, ceftriaxone, aztreonam, ceftazidime and cefotaxime, 10 isolates could be identify els ESBL- E. coli. furthermore 3 out of 10 isolates DDST positives were confirmed ESBL- E. coli using Vitek-2 method.

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Journal Info
journal of Basic Medical Veterinary
Website

Abbrev

JBMV

Publisher

Universitas Airlangga

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, ...