Berkala Penelitian Hayati
Vol 25 No 2 (2020): June 2020

In vitro culture technique of Camellia sinensis L for epicatechin production with phosphor inducer

Sutini Sutini (Department of Agrotechnology, Faculty of Agriculture, UPN ”Veteran” East Java, Surabaya, Indonesia)
Widiwurjani Widiwurjani (Department of Agrotechnology, Faculty of Agriculture, UPN ”Veteran” East Java, Surabaya, Indonesia)
Nora Augustien (Department of Agrotechnology, Faculty of Agriculture, UPN ”Veteran” East Java, Surabaya, Indonesia)
Hadi Suhardjono (Department of Agrotechnology, Faculty of Agriculture, UPN ”Veteran” East Java, Surabaya, Indonesia)
Guniarti Guniarti (Department of Agrotechnology, Faculty of Agriculture, UPN ”Veteran” East Java, Surabaya, Indonesia)
Djoko Agus Purwanto (Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia)
Wirdhatul Muslihatin (Department of Biology, Faculty of Mathematics and Natural Sciences, Sepuluh Nopember Institute of Technology, Surabaya, Indonesia)



Article Info

Publish Date
04 May 2020

Abstract

In several ASEAN countries, in vitro culture techniques are one of the cultivation technologies that applied in sustainable agriculture. This technology has been performed in response to obtain the secondary metabolite of epicatechin from Camellia sinensis L. However, The yield of epicatechin is remaining low than in the field production. To overcome this problem, phosphor inducers is desirable to be supplemented in the study. The young leaf shoots of C. sinensis L was sliced on a culture-filled bottle, the sliced part was re-cultivated in the same medium by supplementing of 2,4-D growth regulators, then the medium was optimized by phosphor inducer with range of concentartions 40-200 ppm. The callus formation was then evaluated using high performance liquid chromatography methods to obtain qualitative and quantitative data. The results showed that the yield of epicatechin was obtained to 11.75%, suggesting that the supplementing of phosphor inducers at cultivation of in vitro culture of C. sinensis L able to improve the production of epicatechin by in vitro culture technique.

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Journal Info

Abbrev

jurnal

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by ...