Muhammadiyah Medical Journal
Vol 2, No 1 (2021): Muhammadiyah Medical Journal (MMJ)

Primers application with the Tso31 gene target in the molecular identification of Taenia solium

Nur Habibah (Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar, Indonesia)
Heri Setiyo Bekti (Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar, Indonesia)
Ni Wayan Rika Kumara Dewi (Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar, Indonesia)
Luh Putu Rinawati (Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar, Indonesia)
Burhannuddin Burhannuddin (Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar, Indonesia)
Aprilia Rakhmawati (Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar, Indonesia)



Article Info

Publish Date
31 May 2021

Abstract

Background: taeniasis is a zoonotic disease caused by Taenia spp. Human taeniasis caused by Taenia solium can be acquired after consumption of raw insufficiently cooked infected pork meat. Pigs are intermediate host for T.solium. Pigs acquired this infection by eating human feces that contained T.solium eggs. Pigs infected with T.solium can be transmitted to humans. Purposes: identification of T.solium in pig is important because it is indicator of T.solium transmission. Microscopic examination of T.solium eggs is considered less effective and efficient so that many other methods are developed for T.solium detection such as molecular and immunology. Method: This method used specific primer which can detect the Tso31 gene in T.solium. Tso31 gene is one of the most promising antigens to differentiate T.solium from T.saginata. Pig feces samples were taken by random sampling technique from 7 pig farms in Denpasar. Result: from the 30 samples, we found one sample that which gave a single amplification product of 234 bp. This indicates that the pig farms in Denpasar have been infected with T.solium. Conclusion: it is necessary to do meat inspection properly in the market as well as health education about the dangers and impacts of T.solium infection in the community.

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Journal Info

Abbrev

MMJ

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Description

Muhammadiyah Medical Journal (MMJ) Faculty of Medicine and Health is an international journal based on scientific research. The journal is published 2 (two) times in 1 year, March and September. Muhammadiyah Medical Journal Faculty of Medicine and Health invites the lecturers, practitioners and ...