Heri Setiyo Bekti
Department Of Medical Laboratory Technology, Health Polytechnic, Ministry Of Health Denpasar, Bali, Indonesia Center Of Excellence In Science And Technology, Health Polytechnic, Ministry Of Health Denpasar, Bali, Indonesia

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KEJADIAN INFEKSI SALURAN KEMIH PADA IBU HAMIL DI DESA DALUNG, KECAMATAN KUTA UTARA, KABUPATEN BADUNG – BALI Ayu Sukma Wedha Kristyanti; I Nyoman Jirna; Heri Setiyo Bekti
Meditory Journal (The Journal of Medcal Laboratory) Vol. 1 No. 2, Desember. 2013
Publisher : Jurusan Analis Kesehatan, Poltekkes Denpasar, Denpasar

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Abstract

Background urinaryTract Infection (UTI) that caused by bacteria is commonly happened in women, such as pregnant women. Untreated UTI can leads to premature birth and low weight in newborn baby. Objective this study aims to determine whether there is any indication of the incidence of UTI in the pregnancy women. Methods research is descriptif study with cross sectional design. Total samples urine 38 from pregnant women at various weeks of gestation who live in the Dalung Village, Sub District of North Kuta, Badung-Bali are cultured. Samples urine that examined are morning urine samples and clean mid-stream urine. Bacterial examination of urine samples is done using colony count method. Results of the study showed that 5 pregnant women (13%) have colony count results more than 100.000 CFU/ml. The colony count results more than 100.000 CFU/ml presences of bacteria in the urine (bacteriuria). Untreated cases of bacteriuria can leads to infections in the urinary tract. Conclusion to pregnancy women to do some laboratory tests if there are some symptoms of UTI and for pregnancy women to be more concerned with the hygiene of urinary tract. Keywords: Colony Count Method; Pregnant Women; Urinary Tract Infection; Urine.
Tinjaun Tentang Perbedaan Waktu Sampling dan Kualitas Bakteriologis Air Isi Ulang di Kecamatan Denpsar Selatan Kota Denpasar Ni Komang Juniawati; Nyoman Mastra; Heri Setiyo Bekti
Meditory Journal (The Journal of Medcal Laboratory) Vol.1, No.1, Juni. 2013
Publisher : Jurusan Analis Kesehatan, Poltekkes Denpasar, Denpasar

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Abstract

Backgroud there is not enough source of water that can be used in Denpasar City especially in South Denpasar District forces the development of refreshment stand drinking water. By refreshment stand drinking water, the people can get drinking water quickly. The quality of the refill drinking water that is being sold effects on the people health who drink it. They must realize it to avoid diseases caused by water such as gastroenteritis (diarrheic, cholera, typhus). Methods this research discuss about the bacteriology quality of the refill drinking water based on time sampling where the time sampling is determined by the row materials changing process. This research was done for 2 weeks by the water samples were taken twice in every seven days. Results the first observation, there were 17 water samples contained 41.2% coliform that is not obey the Indonesian Health Minister Policy No. 492/MENKES/PER/IV/2010, and the second observation, there were 17 water samples samples contained 30% coliform that is not obey the Indonesian Health Minister Policy No. 492/MENKES/PER/IV/2010. By knowing the result of the research, the writer suggests to the people to be able to choose refill drinking water properly and safety. Conclusion Coliform MPN value of drinking water refill time sampling I, 7 samples (41%) exceeds that of 0 (8.8 to 38), so it does not qualify. PMN value refill drinking water coliform sampling period II, 5 samples (30%) exceeds that of 0 (2.2 to 38) so it does not qualify. Bacteriological quality difference is influenced by several factors: the raw water, the cleanliness of the sample bottles, pipettes, media and equipment used. Keywords: time sampling, bacteriology quality, the refill drinking water
Perbedaan Lama Waktu Penundaan Urin Terhadap Hasil Pemeriksaan Glukosa Urin IGA. Dewi Sarihati; Heri Setiyo Bekti; Dewa Ayu Niti Rahayu Putri
Meditory Journal (The Journal of Medcal Laboratory) Vol.1, No.1, Juni. 2013
Publisher : Jurusan Analis Kesehatan, Poltekkes Denpasar, Denpasar

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Abstract

Background the specimen urine usually check is not fresh cause delayed time. The best specimens urine is fresh which is immediately checked. Sample for urine glucose test better by fresh urine. Objective this study to know the difference delayed time check on the result of glucose in urine. Methode the design used in this study is The Postest-Only Control Group Design and the method of sample is purposive sampling. The samples used as 15 samples from 15 different subjects and to do the test as much as three group. The subjects of this study were patients of DM. Statistical tests used the Friedman test with value of α = 0,05. Results of the urine glucose test by fresh urine and delayed time is difference. The followed by Post Hoc test using Wilcoxon. The result in this study between urine glucose test is soon done and delayed time for one hour is no difference p = 1,000 (p > 0,05). There is a difference value between urine glucose test delayed done for one hour and delayed urine glucose test for two hour p = 0,01 (p < 0,05). There is a difference value between urine glucose test is soon done and delayed time urine glucose test for two hour p = 0,01 (p < 0,05). Conclusion the delayed time urine glucose test on two hour was could influenced result. Keywords: delayed time; urine glucose.
Identifikasi Taenia solium secara Mikroskopis pada Peternakan Babi Bekti, Heri Setiyo; Habibah, Nur; Rinawati, Luh Putu; Pradnya Yasa, Ni Putu Candra Dewi; Rindi, Oktavelendi Dhaneta Graha; Dewi, Ni Km Ayu Kusuma; Savitri, Ni Putu Ayu Dani; Rakhmawati, Aprilia
Jurnal Kesehatan Vol 12, No 1 (2021): Jurnal Kesehatan
Publisher : Politeknik Kesehatan Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v12i1.2448

Abstract

Taenia solium is a zoonosis found all over the world, especially in developing countries. T solium can cause taeniasis and cysticercosis in humans. Pigs as intermediate hosts can be infected with T solium which causes porcine cysticercosis. T solium can infect muscle, skin, eye, and brain tissue which can develop into neurocysticercosis. Neurocysticercosis is the cause of 30% of cases of epilepsy in the world. In Denpasar city, pigs are one type of livestock. A large number of pig farms and the Balinese’s people's habit of consuming pork is one of the sources of T solium infection. This research was conducted to determine T solium eggs in pig feces. The study was conducted microscopically on 31 samples with simple random sampling technique. The results showed that of the 31 samples examined, 54.8% (17 samples) were positive for T solium eggs. This indicates that the pig farms in Denpasar city have been infected with T solium. Pig farming in Denpasar city was done traditionally and kept indoors. However, the breeders pay less attention to pig health and environmental sanitation. Also, pigs are slaughtered privately by breeders because there are no slaughterhouses. Lack of supervision of pork circulating in the community is also the cause of T solium infection. Therefore, it is necessary to carry out supervision by the local government on pig farms as well as supervision of pork consumed by the public to prevent and eradicate T solium infection.
Overview of Visfatin Levels in Pregnant Women as a Stunting Biomarker Ni Nyoman Astika Dewi; Luh Putu Rinawati; Ni Putu Ditya Prabandari Dewi; I Made Krishna Paramartha; Ni Putu Intan Maha Ayu Diyanti; Aprilia Rakhmawati; Heri Setiyo Bekti
JURNAL KEBIDANAN Vol 12, No 1 (2022): April 2022
Publisher : Politeknik Kesehatan Kementerian Kesehatan Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkb.v12i1.8018

Abstract

Stunting is one of the malnutrition problems experienced by toddlers in the world. Indonesia is the third country in the prevalence of stunting in the Southeast Asian region. Visfatin is one of the molecules that play a role in fetal development during the womb. Reports related to visfatin in pregnant women are still very few, and mention visfatin plays a role in the incidence of preeclampsia (PE), fetal growth restriction, and gestational diabetes mellitus (GDM). This study aims to determine the level of visfatin in pregnant women as a biomarker of stunting in Bangli Regency, Bali Province. This research is a descriptive study, with an observational design. Blood samples were taken from the median cubital vein. Samples were examined by ELISA method. In the examination of vistafin levels in 30 pregnant women, the lowest level being 0.53 ng/ml and the highest level being 56.72 ng/ml. From the results obtained and compared with previous studies, the visfatin levels obtained were lower than the visfatin levels in normal pregnant women. Many studies have shown that increased levels of visfatin in maternal plasma are associated with low birth weight and intrauterine growth restriction (IUGR). Babies born with LBW have a risk of stunting.
Primers application with the Tso31 gene target in the molecular identification of Taenia solium Nur Habibah; Heri Setiyo Bekti; Ni Wayan Rika Kumara Dewi; Luh Putu Rinawati; Burhannuddin Burhannuddin; Aprilia Rakhmawati
Muhammadiyah Medical Journal Vol 2, No 1 (2021): Muhammadiyah Medical Journal (MMJ)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.651 KB) | DOI: 10.24853/mmj.2.1.35-40

Abstract

Background: taeniasis is a zoonotic disease caused by Taenia spp. Human taeniasis caused by Taenia solium can be acquired after consumption of raw insufficiently cooked infected pork meat. Pigs are intermediate host for T.solium. Pigs acquired this infection by eating human feces that contained T.solium eggs. Pigs infected with T.solium can be transmitted to humans. Purposes: identification of T.solium in pig is important because it is indicator of T.solium transmission. Microscopic examination of T.solium eggs is considered less effective and efficient so that many other methods are developed for T.solium detection such as molecular and immunology. Method: This method used specific primer which can detect the Tso31 gene in T.solium. Tso31 gene is one of the most promising antigens to differentiate T.solium from T.saginata. Pig feces samples were taken by random sampling technique from 7 pig farms in Denpasar. Result: from the 30 samples, we found one sample that which gave a single amplification product of 234 bp. This indicates that the pig farms in Denpasar have been infected with T.solium. Conclusion: it is necessary to do meat inspection properly in the market as well as health education about the dangers and impacts of T.solium infection in the community.
The Effectiveness of Green Grape Extract (Vitis vinifera) on Decreasing White Rat (Rattus novergicus) Triglycerides Levels Ketut Lilik Arwati; Ni Nyoman Astika Dewi; Heri Setiyo Bekti; Ni Wayan Rika Kumara Dewi; Andi Udin Saransi
Muhammadiyah Medical Journal Vol 3, No 1 (2022): Muhammadiyah Medical Journal (MMJ)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mmj.3.1.1-9

Abstract

Background: Grapes (Vitis vinifera) are a source of antioxidants with high content of polyphenols and anthocyanins. Grape skin is rich in flavonoids. Anthocyanins and flavonoids have the effect of lowering blood triglyceride levels. Purposes: This study aimed to determine the effect of using green grape extract (GGE) in lowering triglyceride levels in the blood. Methods: Five groups of white male rats (Rattus novergicus) were divided into a negative control group (KN), positive control (KP), rats with a GGE dose of 100 mg/200g BW as P1, rats with a GGE dose of 250mg/200g BW as P2, and rats with GGE dose of 500 mg/200g BW as P3. Each group was given a hypercholesterolemic diet for five weeks. Groups P1, P2, and P3 were given GGE according to their respective doses for 14 days, then measured triglyceride levels in the blood. Results: The results showed that GGE 500mg/200g BW significantly reduced triglyceride levels in all treated mice. Conclusion: These results indicate that GGE has a great potential to treat dyslipidemia by lowering triglyceride levels in the blood.
GAMBARAN KADAR SERUM INTERLEUKIN-6 PADA PEROKOK AKTIF I Gusti Agung Dewi Sarihati; Kadek Ika Surya Cahyani; Ida Ayu Sri Arjani; Surya Bayu Kurniawan; Heri Setiyo Bekti
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1282

Abstract

AbstractCigarette smoke contains Reactive Oxygen Species (ROS). ROS when it is over-produced causes  oxidative stress. Oxidative stress is related to the synthesis of pro-in-flamatory cytokines  which encourage inflammation which is characterized by the increase in proinflammatory cytokines. The purpose of this study was to determine the description of serum interleukin-6 levels in active smokers. The research sample was 11 active smokers in the Bali Regional Police who were selected using a non- probability sampling technique using purposive sampling. Measurement of serum interleukin-6 levels was performed using the ELISA method. The results showed that serum interleukin-6 levels increased in all age groups and most were found at the age of 35-44 years (36.3%), with normal and obese BMI each 45.5%, in white cigarette smokers with filters (72.7%), moderate smoking intensity (63.6%), with smoking duration of 11-15 years (36.4%). The conclusion in this study is that most active smokers have increased serum interleukin-6 levels. Keywords: active smokers, serum, interleukin-6
Daya Hambat Ekstrak Daun Sirih dan Daun Legundi Terhadap Pertumbuhan Bakteri Staphylococcus aureus Ida Bagus Oka Suyasa; Heri Setiyo Bekti; Luh Putu Rinawati; Luh Putu Laksmi; Putu Diah Wahyuni; Desak Gede Dwi Agustini; Aprilia Rakhmawati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.11015

Abstract

Staphylococcus aureus is one of the causes of infectious disease, ranging from mild to severe infections such as meningitis. The drug that is often used is the penicillin class of antibiotics. Apart from chemical antibiotics, natural antibacterial compunds from plants can be also used as an alternative treatment for this infection. Such as green betel (Piper betle L.) and legundi (Vitex trifolia L.). The ethanol extract of betel leaf has antibacterial activity against Staphylococcus aureus. The phytochemical content in legundi leaf is also able to inhibit the growth of Staphylococcus aureus. The ethanol extract of betel leaf and legundi is made through a maceration process. The extract concentrations used in this study were 20%, 30%, and 40%. The inhibition test of Staphylococcus aureus using the disc method. From the results of this study, the active substances found in betel leaf were flavonoids, tannins, and phenols, while in legundi were found flavonoids, tannins and phenols, and quinones. In total, the mean inhibition zone in this study ranged from 7.87mm to 17.33mm where legundi leaf extract was in the moderate category, betel and combination extract were classified as strong. There was a difference in the diameter of the inhibition zone of betel and legundi extract at a concentration 20% with 40%. In the combination extract there is a difference in the concentration of 20% with 40% and 30% with 40%. An antimicrobial compound can be said to synergize if a mixture of two or more antimicrobial compounds is able to provide an effect of each compounds. In this study, it has seen that the mean of the combination is higher than the mean of legundi leaf extract. Meanwhile, the mean of betel leaf extract is still greater than the combination extract.
Uji Aktivitas Antibakteri Cuka Apel Terhadap Bakteri Salmonella Paratyphi A I Gusti Ayu Made Wulan Diantari; Heri Setiyo Bekti; Cokorda Dewi Widhya Hana Sundari
Jurnal Skala Husada : The Journal of Health Vol 19, No 1 (2022): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v19i1.1944

Abstract

ABSTRACTSalmonella paratyphi A causes enteric fever, which is paratyphic fever which attacks the intestines and is acute. Apple cider vinegar contains acetic acid, apple cider vinegar contains organic acids, namely acetic acid and amino acids, flavonoids, polyphenols, pectins and it’s also rich of vitamins and minerals. The acetic acid content of apple cider vinegar acts as an antimicrobial. The objective of the research was to determine the concentration of apple cider vinegar which was effective in inhibiting the growth of Salmonella paratyphi A bacteria. The type of research used was true experiment using posttest only control group design. The results obtained are apple vinegar can inhibit the growth of Salmonella paratyphi A bacteria with the result that the inhibition zone diameter at a concentration of 15% is 8,8 mm, 20% is 11,22 mm, 25% is 11,92 mm, and 30% is 13,05 mm, whereas in the positive control group it was obtained 29,95 mm and the negative control group was 0 mm or no inhibition zone is formed. The different test using Kruskal Wallis resulted in a value of sig (p) (0.05) so it indicated that there were differences in the inhibition zone for the growth of Salmonella paratyphi A bacteria against various concentrations of apple cider vinegar. It was concluded that there was an antibacterial activity of apple cider vinegar against the growth of Salmonella paratyphi A bacteria and the effective concentration that could inhibit the growth of Salmonella paratyphi A bacteria was at 30% concentration.ABSTRAKSalmonella paratyphi A menyebabkan demam enterik yaitu demam paratifus yang menyerang usus dan bersifat akut. Cuka apel memiliki kandungan asam asetat, Cuka sari apel memiliki kandungan asam organik yaitu asam asetat dan asam amino, flavonoid, polifenol, pektin serta kaya vitamin dan mineral. Kandungan asam asetat yang dimiliki cuka sari apel bertindak sebagai antimikroba. Tujuan penelitian adalah untuk mengetahui konsentrasi cuka apel yang efektif untuk menghambat pertumbuhan bakteri Salmonella paratyphi A. Jenis penelitian yang digunakan adalah true experiment dengan menggunakan rancangan posttest only control group design. Uji daya hambat antibakteri dengan menggunakan empat macam konsentrasi yaitu 15%, 20%, 25%, dan 30%. Sedangkan kontrol kerja yang digunakan adalah Kloramfenikol 30 µg sebagai kontrol positif dan aquadest steril sebagai kontrol negatif. Hasil yang didapatkan adalah cuka apel dapat menghambat pertumbuhan dari bakteri Salmonella paratyphi A dengan hasil diameter zona hambat pada konsentrasi 15% sebesar 8,8 mm, 20% sebesar 11,22 mm, 25% sebesar 11,92 mm, dan 30% sebesar 13,05 mm, sedangkan pada kelompok kontrol positif didapatkan sebesar 29,95 mm dan kontrol negatif sebesar 0 mm. Uji beda dengan uji Kruskal Wallis memperoleh hasil nilai sig (p) (0,05) sehingga menunjukkan adanya perbedaan zona hambat pertumbuhan bakteri Salmonella paratyphi A terhadap berbegai konsentrasi dari cuka apel. disimpulkan bahwa ada aktivitas antibakteri cuka apel terhadap pertumbuhan bakteri Salmonella paratyphi A dan konsentrasi efektif yang dapat menghambat pertumbuhan bakteri Salmonella paratyphi A adalah konsentrasi 30%.