Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Vol. 8 No. 2 (2021): December 2021

PENGARUH CEKAMAN KEKERINGAN TERHADAP EKSPRESI GEN KETAHANAN OSCATA DAN OSAPX1 PADA PADI TOLERAN KEKERINGAN

Fariza Oktaviani (Program Studi Magister Bioteknologi, Universitas Jember, Jl. Kalimantan, Kampus Tegal Boto, Jember 68121, Indonesia)
Irma Novita Sari (Program Studi Agroteknologi, Universitas Jember, Jl. Kalimantan, Kampus Tegal Boto, Jember 68121, Indonesia)
Tri Handoyo (Pusat Unggulan Bioteknologi Tanaman Industri (PUI-PT BioTIn), Universitas Jember, Jl. Kalimantan, Kampus Tegal Boto, Jember 68121, Indonesia)
Tri Agus Siswoyo (Program Studi Magister Bioteknologi, Universitas Jember, Jl. Kalimantan, Kampus Tegal Boto, Jember 68121, Indonesia)
Mohammad Ubaidillah (Program Studi Agroteknologi, Universitas Jember, Jl. Kalimantan, Kampus Tegal Boto, Jember 68121, Indonesia)



Article Info

Publish Date
28 Dec 2021

Abstract

Rice is widely cultivated in Indonesia, where one of the problems is drought. Rice plant growth can be inhibited due to a lack of water, which could cause oxidative stress. One mechanism for self-defence involves activating antioxidative genes. This study aims to determine the regulatory response of OsCATA and OsAPX1 resistance genes in rice under drought stress. The rice varieties used were those already pre-tested, including Siak Raya, Sertani 1, Indragiri, IR64. Drought treatments were 0% (control) and 15% PEG 6000. Results indicated that the interaction of rice variety treatment and drought stress had a highly significant effect on the root length and chlorophyll content but no significant effect on the plant height. Also, the gene expression of OsCATA and OsAPX1 increased in rice plants exposed to drought stress. Variety Sertani 1 was recommended due to their high average increase in root length, reduced average plant height and chlorophyll content, as well as increased gene expression in conditions of drought stress. Padi banyak dibudidayakan di Indonesia, di mana salah satu permasalahannya adalah kekeringan. Pertumbuhan tanaman padi dapat terhambat akibat kekurangan air, yang dapat menyebabkan terjadinya stres oksidatif. Salah satu mekanisme pertahanan diri adalah mengaktifkan gen antioksidatif. Penelitian ini bertujuan mengetahui respons regulasi gen ketahanan OsCATA dan OsAPX1 pada padi yang mengalami cekaman kekeringan. Benih varietas padi yang digunakan sudah diuji pendahuluan sebelumnya, antara lain Siak Raya, Sertani 1, Indragiri, IR64. Perlakuan kekeringan yaitu 0% (kontrol) dan 15% PEG 6000. Hasil penelitian menunjukkan interaksi perlakuan varietas padi dengan cekaman kekeringan signifikan terhadap panjang akar, dan total klorofil namun tidak signifikan terhadap tinggi tanaman. Ekspresi gen OsCATA dan OsAPX1 meningkat pada tanaman padi yang mengalami cekaman kekeringan. Tanaman yang direkomendasikan adalah varietas Sertani 1 yang memiliki peningkatan rata-rata panjang akar yang tinggi, penurunan rata-rata tinggi tanaman, dan kandungan klorofil rendah, serta meningkatnya ekspresi gen pada kondisi cekaman kekeringan.

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Journal Info

Abbrev

JBBI

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review ...