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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Pangan dan Gizi Jurnal AGROTEKNOLOGI Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) ARTERI : Jurnal Ilmu Kesehatan Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Nasional (JPN) Indonesia

Mohammad Ubaidillah

Center For Development Of Advanced Science And Technology (CDAST), Jember University, Jember, East Java, Indonesia

Author-ID : 3235270

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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RESPON PEMBERIAN GIBERELIN TERHADAP KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN PADA BUAH TANAMAN CIPLUKAN (Physalis angulata L.) Fitra Dea Wafa; Mohammad Ubaidillah; Tri Agus Siswoyo
JURNAL AGROTEKNOLOGI Vol 15 No 02 (2021)
Publisher : Faculty of Agricultural Technology, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/j-agt.v15i02.25391

Ciplukan (Physalis angulata L.) is one of the plants from the class of herbal plants that is good to be developed because people are starting to choose herbal treatment. The content of ciplukan fruit is phenolic and flavonoid. Thus, technology for enlargement and increasing fruit content with the giberellin (GA3) hormone is needed. GA3 functions to stimulate the formation, development of fruit and increase the biosynthesis of secondary metabolites. This study aimed to determine the influence of the GA3 hormone on the phenolic content and antioxidant activity in ciplukan fruit. The experimental design used RAL 5 treatments: GA3 concentrations of 0 ppm (P0), 15 ppm (P1), 20 ppm (P2), 25 ppm (P3), and 30 ppm (P4). The parameters studied were the number of fruit, fruit size, fresh weight of fruit, total phenolic, total flavonoid, and antioxidant activity (DPPH method) in ciplukan fruit. The results showed that the concentration of 30 ppm (P4) of GA3 was showed the highest values on the number of fruits (33±3.53 g), fruit length (14.23±0.35 mm), width (12.87±0.39 mm), and fresh fruit weight (38.6±3.97 g) without calyx. The concentration of 30 ppm (P4) also affected the phenolic content (2.52±0.23 mgGAE/g), flavonoids (2.43±0.18 mgQE/g), and IC50 value of antioxidant activity (60.59 g/mL). Keywords: antioxidant activity, ciplukan, flavonoid, giberellin, phenolic

PENGARUH CEKAMAN KEKERINGAN TERHADAP EKSPRESI GEN KETAHANAN OSCATA DAN OSAPX1 PADA PADI TOLERAN KEKERINGAN Fariza Oktaviani; Irma Novita Sari; Tri Handoyo; Tri Agus Siswoyo; Mohammad Ubaidillah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Rice is widely cultivated in Indonesia, where one of the problems is drought. Rice plant growth can be inhibited due to a lack of water, which could cause oxidative stress. One mechanism for self-defence involves activating antioxidative genes. This study aims to determine the regulatory response of OsCATA and OsAPX1 resistance genes in rice under drought stress. The rice varieties used were those already pre-tested, including Siak Raya, Sertani 1, Indragiri, IR64. Drought treatments were 0% (control) and 15% PEG 6000. Results indicated that the interaction of rice variety treatment and drought stress had a highly significant effect on the root length and chlorophyll content but no significant effect on the plant height. Also, the gene expression of OsCATA and OsAPX1 increased in rice plants exposed to drought stress. Variety Sertani 1 was recommended due to their high average increase in root length, reduced average plant height and chlorophyll content, as well as increased gene expression in conditions of drought stress. Padi banyak dibudidayakan di Indonesia, di mana salah satu permasalahannya adalah kekeringan. Pertumbuhan tanaman padi dapat terhambat akibat kekurangan air, yang dapat menyebabkan terjadinya stres oksidatif. Salah satu mekanisme pertahanan diri adalah mengaktifkan gen antioksidatif. Penelitian ini bertujuan mengetahui respons regulasi gen ketahanan OsCATA dan OsAPX1 pada padi yang mengalami cekaman kekeringan. Benih varietas padi yang digunakan sudah diuji pendahuluan sebelumnya, antara lain Siak Raya, Sertani 1, Indragiri, IR64. Perlakuan kekeringan yaitu 0% (kontrol) dan 15% PEG 6000. Hasil penelitian menunjukkan interaksi perlakuan varietas padi dengan cekaman kekeringan signifikan terhadap panjang akar, dan total klorofil namun tidak signifikan terhadap tinggi tanaman. Ekspresi gen OsCATA dan OsAPX1 meningkat pada tanaman padi yang mengalami cekaman kekeringan. Tanaman yang direkomendasikan adalah varietas Sertani 1 yang memiliki peningkatan rata-rata panjang akar yang tinggi, penurunan rata-rata tinggi tanaman, dan kandungan klorofil rendah, serta meningkatnya ekspresi gen pada kondisi cekaman kekeringan.

SEGREGATION ANALYSIS OF MORPHOLOGICAL CHARACTER AND BIOACTIVE GENOTYPE OF RICE PLANT F3 (CEMPO SALAMET/IR64) POPULATION Indri Gita Lestari; Mohammad Ubaidillah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Red rice contains high anthocyanin and bioactive antioxidant compounds that prevent free radical reactions. Cempo Salamet has potential as an antioxidant source, and the characteristics are red colored grains, 4–5 months old, 169 cm plant height, 7 productive tillers per plant, and resistance to blast disease. IR64 had been developed with the following characteristics: 3 months old, 85 cm plant height, 20–35 productive tillers per plant, resistance to brown leafhoppers pigment. This study aimed to obtain information on the segregation of the F3 population from crosses between the Cempo Salamet and IR64 varieties. Research methods included preparation and maintenance with genotype analysis. PCR analysis was conducted using SSR markers with primer RM346, RM316, RM228, and RM339. The segregation in F3 plants was 50% for >130 cm plant height, 51% for 10–19 tillers per plant, 67% for 2.2 g/100-grain weight, and 33% strong red for colour intensity. The findings demonstrated that SSR markers RM346, RM339, and RM228 could validate Cempo Salamet, IR64, and F3 DNA bands. However, RM316 could not validate all DNA bands in the research sample. Beras merah mengandung antosianin yang tinggi dan senyawa bioaktif antioksidan yang mampu mencegah terjadinya reaksi radikal bebas. Cempo Salamet berpotensi sebagai sumber antioksidan yang memiliki karakteristik biji berwarna merah, umur 4–5 bulan, tinggi tanaman 169 cm, anakan produktif 7 batang, tahan terhadap penyakit blas. IR64 telah banyak dibudidayakan dengan karakteristik umur 3 bulan, tinggi tanaman 85 cm, anakan produktif 20–35 batang, tahan terhadap wereng coklat, akan tetapi tidak mengandung pigmen. Penelitian ini bertujuan mendapatkan informasi segregasi populasi F3 hasil persilangan antara varietas Cempo Salamet dan IR64. Metode penelitian meliputi persiapan dan pemeliharaan tanaman serta analisis genotipe bioaktif. Analisis PCR menggunaan marka SSR dengan primer RM 346, RM 316, RM 228, dan RM 339. Terjadi segregasi karakter morfologi pada populasi F3 yaitu diperoleh tanaman dengan tinggi >130 cm (50%), 10–19 anakan (51%), bobot 100 bulir dengan 2,2 g (67%), dan intensitas warna bulir dengan merah kuat (33%). Penelitian menunjukkan marker SSR yang dapat memvalidasi pita-pita DNA yaitu RM346, RM339, and RM228, sedangkan RM316 tidak dapat memvalidasi keseluruhan pita-pita DNA pada sampel penelitian.

Analisis Kandungan Gizi Beras dari Beberapa Galur Padi Transgenik Pac Nagdong/Ir36 Dina Fitriyah; Mohammad Ubaidillah; Fariza Oktaviani
ARTERI : Jurnal Ilmu Kesehatan Vol 1 No 2 (2020): Februari
Publisher : Puslitbang Sinergis Asa Professional

Rice that is generally consumed by Indonesian people is that does not have important nutrient such as β-carotene. Therefore, the way to supply β-carotene requirement in staple foods can be done through β-carotene biofortification by genetic engineering of the rice plant. PAC Nagdong/ IR36 is transgenic Golden Rice which can produce β-carotene. The research aimed to determine some nutritional content of PAC Nagdong/ IR36 rice lines that include carbohydrate, amylose, amylopectin, protein, lipid, and β-carotene content. Amylose was measured using the iodine colorimetric method, whereas the contents of amylopectin and carbohydrates were measured using the by difference method. Protein was measured using the Bradford method, lipid was measured using Proctor and Bowen method, while β-carotene content was measured using the colorimetric method. PAC Nagdong/ IR36 rice lines had carbohydrate content between 85,06-90,16%, amylose 12,94%- 20,81%, amylopectin 69,35- 76,17%, protein 7,76-8,52 µg/µl, lipid 0,65-0,90%, and β-carotene 231,61-920,52 µg/g. Carbohydrate, protein, and β-carotene in PAC Nagdong/ IR36 were higher than TN1 rice variety (control). This indicated that PAC Nagdong/ IR36 provided better nutrition. Amylose content of Nagdong/ IR36 PAC rice was low, which donated its potential to produce fluffier rice whereas high β-carotene content could be used as a source of β-carotene that is useful in overcoming vitamin A deficiency and beneficial for health including in preventing degenerative diseases.

Analisis Kandungan Senyawa Bioaktif, Nutrisi dan Aktivitas Antioksidan pada Minuman Ekstrak Beras Hitam Dina Fitriyah; Dessya Putri Ayu; Surya Dewi Puspita; Yohan Yuanta; Mohammad Ubaidillah
ARTERI : Jurnal Ilmu Kesehatan Vol 3 No 1 (2021): November
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v3i1.204

Black rice contains high bioactive compounds that have antioxidant activity so that it has the potential as a source of functional food that can help to overcome various health problems. Black rice extract has high potential to be developed into an antioxidant beverage. Research on the content of bioactive compounds, antioxidant activity, and nutrients in black rice extract beverages has not been widely studied in Indonesia. The objective of this study was to determine the content of bioactive compounds, activity, and nutrients of black rice extract. Four types of commercial black rice grains were extracted using methanol as comparison (without treatment) and using water with a temperature of 90°C for 15 and 25 minutes, with the ratio of water: rice powder (20, 25 and 30 ml/ g). The black rice extracts were tested for total phenolic compound, antioxidant activity, protein, and iron content. The highest total phenolic content, flavonoids, and antioxidant activity of black rice extract beverages were 4.54 mg GAE/g (water ratio: rice powder 20 ml/g, 15 minutes), 14.51 mg QE/g (water ratio: rice powder 20 ml/g, 15 minutes), and 73.44% (water ratio: rice powder 30 ml/g, 15 minutes). The total protein and iron content of extract black rice beverage was 0.3115- 0.3620% and 0.0100- 0.0123%, respectively. The presence of high content of bioactive compounds, protein, iron, and antioxidant activity in black rice extract with a processing time of 15 and 25 minutes at 90°C, and the ratio of water: rice powder (20, 25, 30 ml/g) indicates that the processing conditions can provide a reference in producing black rice extract beverages which are rich in bioactive compounds, antioxidants, and nutrients. The presence of iron in brown rice extract may help overcome iron deficiency.

Morphological, Physiological and Molecular Characteristics of Tolerant Sugarcane to Waterlogging Stress Viki Bayu Wibisono; Sholeh Avivi; Mohammad Ubaidillah; Sri Hartatik
Indonesian Journal of Agronomy Vol. 50 No. 2 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Waterlogging stress reduced sugarcane yield by 15-45%. This study aimed to determine the morphological, physiological, and molecular response of sugarcane under waterlogging stress. This research was conducted from October 2021 to January 2022 at the Agrotechnology Departement Laboratory, Faculty of Agriculture, Jember University, East Java. The experiment was arranged using a randomized block factorial design. The first factor was the genotype of sugarcane with 6 levels (Bululawang, PS 862, Cening, PS 881, M3, M4). The second factor was the waterlogging period with 3 levels (30 DAT, 60 DAT, and 90 DAT). The third factor was waterlogging treatment (without waterlogging treatment and with waterlogging treatment). Several growth characteristics such as fresh root weight, stem diameter, plant height, leaf area surface, aerenchyma tissue, total chlorophyll, stomata density, H2O2, and antioxidant gene expression were investigated. The results showed that waterlogging stress had an impact on the formation of aerenchyma tissue, plant height, fresh root weight, leaf area, total chlorophyll, stomata density, hydrogen peroxide, and expression of antioxidant gene. Cening genotype showed the best response in terms of low decreasing percentage to morphological, physiological, and high antioxiant gene expression. Keywords: antioxidant, flooding, resistance, reactive oxygen species, Cening

Kandungan Nutrisi dan Aktivitas Antimikroba Ekstrak Beras Merah Dina Fitriyah; Dessya Putri Ayu; Surya Dewi Puspita; Ria Chandra Kartika; Mohammad Ubaidillah
Jurnal Pangan dan Gizi Vol 12, No 2 (2022): Kajian Pangan dan Gizi
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jpg.12.2.2022.30 - 36

Red rice is an important commodity with antioxidant activity. The antioxidant content in red rice can help to overcome various health problems. Red rice extract has the potential to be a functional drink that is rich in nutrients and antioxidants. In Indonesia, studies regarding the nutritional content and antimicrobial activity of red rice extract are still few and poorly explored. This study aims to determine nutritional content and potency of antimicrobial red rice extract. Four types of commercial red rice were extracted using a treatment of 90°C of temperature, 15 minutes, with the ratio of water: rice 1: 20 ml/g. The conventional extraction method using methanol was used as a comparison. The extract was measured for protein content, iron, and antimicrobial activity. Red rice extract's total protein and iron content were 0.3305-0.5660% and 0.0090-0.0141%, respectively. Red rice extract concentrated up to 100 µl can inhibit Staphylococcus aureus and Escherichia coli growth with the largest inhibition zone of 3 mm and an antimicrobial index of 0.5. The protein content of red rice extracted with our method showed a higher value when compared to conventional extraction using methanol. The presence of protein, iron, and antimicrobial activity in red rice extract can be potential as a functional drink that is good for health because the presence of iron in red rice extract has the potential to overcome iron deficiency. Moreover, the antimicrobial activity in red rice can be beneficial to inhibit the growth of bacteria that cause foodborne diseases such as S. aureus and E. coli.

Uji Aktivitas Antimikroba Hasil Ekstraksi dari Padi Lokal Indonesia Lana Yunita; Mohammad Ubaidillah
Agriprima : Journal of Applied Agricultural Sciences Vol 6 No 2 (2022): SEPTEMBER
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v6i2.471

Daun padi merupakan salah satu organ tanaman padi yang mengandung senyawa bioaktif yang berguna bagi kesehatan. Senyawa bioaktif fenolik adalah salah satu turunan dari antosianin yang merupakan pigmen warna pada daun padi. Senyawa fenolik memiliki sifat antioksidan alami yang berpotensi sebagai antimikroba. Tujuan penelitian ini yaitu melakukan analisis senyawa bioaktif ekstrak daun padi berpigmen dan menganalisis pengaruhnya terhadap aktivitas penghambatan mikroba. Penelitian ini dilakukan pada bulan Juli – Desember 2021, di Laboraturium Agroteknologi, Laboraturium Agronomi Fakultas Pertanian dan Laboraturium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember. Metode penelitian yang dilakukan yaitu melakukan ekstraksi untuk analisis kandungan total fenolik, kadar antioksidan zona hambat untuk bakteri E.coli dan S. aureus. Perlakuan konsentrasi fenolik yang digunakan untuk melihat zona hambat bakteri yaitu 0,19 µg/ml; 0,095 µg/ml; 0,048 µg/ml; 0,024 µg/ml dan kontrol yaitu ampicilin 100 ppm dan air. Data hasil penelitian dianalisis menggunakan ANOVA dan uji lanjut DMRT 5 %. Kandungan total fenolik untuk tiap varietasnya berbeda dan total fenolik tertinggi terdapat pada varietas Gogo Niti II yaitu 0,89 µg/ml sedangkan kadar antioksidan tertinggi yaitu Merah Wangi sebesar 76,16%. Rerata zona hambat bakteri E.coli tertinggi terdapat pada perlakuan 0,19 µg/ml varietas Black Madras dan rerata zona hambat bakteri S.aureus tertinggi pada ampicilin 100 ppm pada varietas Black Madras dibandingkan dengan varietas yang lain sedangkan varietas Merah Saleman tidak membentuk zona hambat pada perlakuan manapun kecuali ampicilin 100 ppm.

Karakterisasi Morfologi dan Molekuler Galur Padi Introduksi Golden Rice F8 terhadap Penyakit Blas dan Hawar Daun Bakteri Viki Bayu Wibisono; Dwi Mai Abdul Imam Buqori; Danny Agus Dyanshah; Sri Hartatik; Mohammad Ubaidillah
Indonesian Journal of Agronomy Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Golden rice merupakan padi yang di dalam endosperma bijinya memiliki kandungan Î²-karoten atau provitamin A. Beberapa upaya telah dilakukan untuk meningkatkan ketahanannya terhadap penyakit blas dan hawar daun bakteri (HDB). Hasil pemuliaan Golden rice telah mendapatkan turunan ke 8 (F8) yang bersifat stabil. Penelitian ini bertujuan untuk mengkarakterisasi agromorfologi dan genotipe galur padi introduksi Golden rice F8 PAC Nagdong/IR36/IR64. Penelitian dilakukan di Laboratorium Agroteknologi, Fakultas Pertanian, Universitas Jember. Benih yang digunakan yaitu tiga galur padi intorduksi Golden rice F8 PAC Nagdong/IR36/IR64 dengan kode 302/IR-2-2(6)/1, 302/IR-2-2(7)/2, 302/IR-2-2(8)/1 serta varietas IR36 dan IR64 sebagai tanaman kontrol. Analisis genotipe meliputi gen Pik-s, Pi54, Pi-ta, Xa4 dan Xa7. Hasil penelitian menunjukkan bahwa karakter agromorfologi pada seluruh galur Golden rice untuk karakter tinggi tanaman, jumlah anakan dan jumlah anakan produktif tidak berbeda nyata. Tinggi tanaman Golden rice termasuk dalam kategori semidwarf, jumlah anakan >24.6 dengan anakan produktif mencapai lebih dari 85%, panjang malai antara 20.09-20.85 cm, dan bobot per rumpun mencapai 42.94 g serta seluruh galur Golden rice memiliki gen pengkode sifat tahan terhadap penyakit blas dan HDB. Kata kunci: Î²-karoten, galur, HDB, introduksi, marka

Pengaruh BAP, IAA, dan Jenis Eksplan terhadap Efisiensi Regenerasi Tomat Fortuna 23 Sholeh Avivi; Mohammad Ubaidillah; Setiyono; Rifngatul â€˜Atiqoh
Indonesian Journal of Agronomy Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

This study aimed to determine an efficient regeneration protocol for genetic transformation of tomato Fortuna 23. This study was conducted from August 2021 to February 2022 at the Tissue Culture Laboratory, Faculty of Agriculture, University of Jember. The study consisted of 2 experimental designs, i.e, a completely randomized factorial design for shoot induction study with three factors and three replications and a completely randomized non-factorial design for root induction study with five replications. The treatments for shoot induction study were BAP (0, 1, 2, and 3 mg L-1), IAA (0, 0.1, 0.2, and 0.3 mg L-1), and types of explants (cotyledons, hypocotyls, and roots). The treatments for root induction study were NAA (0, 0.1, 0.5, and 1 mg L-1). The combination treatment B2A3E1 (BAP 2 mg L-1 + IAA 0.3 mg L-1 + cotyledons) was the best treatment for shoot induction since it was able to produce the highest number of shoots (8 shoots), with shoots appearing fast (21 HSI) and callus appearing fast too (7 HSI). NAA 0.5 mg L-1 resulted in the fastest root emergence (3 HSI), the highest number of leaves (5 leaves), and the highest number of roots (26). Keywords: regeneration, root induction, shoot induction

Co-Authors Adelia Niandhita Palupi Adhitya Wardhono Agung Nugroho Puspito Agung Nugroho Puspito Aisyah Ayu Nuril Firdausi Akbar Arie Yuhdie Andini Dwi Fitriana Atiqa Zahra Aulia Aulia Rafidah Pananto Ciplis Gema Qori’ah Clara Sintha Putri Pratiwi Daniyah Basalamah Danny Agus Dyanshah Dessya Putri Ayu Dessya Putri Ayu Dina Fitriyah Dina Fitriyah Dina Fitriyah Dini Nurin Alfiyah Dodik Priyanto Dwi Candra Nugraha Dwi Mai Abdul Imam Buqori Dwi Mai Abdul Imam Buqori Fajri Qothrunnada Fariza Oktaviani Fariza Oktaviani Farokhatun Nazilah Fitra Dea Wafa Hasbi Mubarak Suud Ika Purnamasari Indri Gita Lestari Iqbal Bayu Ferdiansah Irma Novita Sari Keysa Churiyyatul Haris Khansa Alura Ramadhina Lana Yunita Livia Ika Lindari M Andik Fathur Rohman M. Alif Muzhaffar Mellfani Rhamadinda Nendra Tigara Muchammad Savero Sulthan Marzi Nabila Safira Putri Madani Naiya Nalya Cahyadi Ria Chandra Kartika Rifngatul â€˜Atiqoh Setiyono Shofia Dwi Rahma Wati Sholeh Avivi Sholeh Avivi Sri Hartatik Sri Hartatik Surya Dewi Puspita Surya Dewi Puspita Telpivani Solehatul Putri Untari Tri Agus Siswoyo TRI HANDOYO Viki Bayu Wibisono Viki Bayu Wibisono Yohan Yuanta

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