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KARAKTERISTIK PERUBAHAN PROTEIN BIJI MELINJO (GNETUM GNEMON) PADA AWAL PERKECAMBAHAN Siregar, Adrian Syawaluddin; Siswoyo, Tri Agus; Sukowardojo, Bambang
Berkala Ilmiah Pertanian (e-ISSN: 2338-8331) Vol 1, No 2: NOVEMBER
Publisher : Berkala Ilmiah Pertanian (e-ISSN: 2338-8331)

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Abstract

[ENGLISH] Protein was one of macromolecule in seed which played an important role in the process of germination. Storage protein in seed was hydrolyzed to amino acid which are then used in the translation process for a genetic message interpretating and protein building in pre-germination process. Pre-Germination was the first step of germination that have many biochemical changes. The objectives of this research was to know biochemical characteristic of soluble protein and protein pattern of melinjo seed during pre-germination. Two varieties of melinjo seed Gentong and Kerikil was used in this research with three stages of treatment (initial seed, 3 weeks warm stratification seed and 1 month seed seedling). Seed samples are extracted to determine total soluble protein and protein pattern with 15 % SDS-PAGE. The results showed that total soluble protein have a change different at each stage of the treatment. Protein patterns analysis indicates band protein with molecular weight 66 kDa and 14 kDa change during pre-germination. Keywords: Melinjo Seed; Pre-Germination; Protein; SDS-PAGE [INDONESIAN] Protein merupakan salah satu makromolekul dalam biji yang berperan penting pada proses perkecambahan. Protein yang tersimpan pada biji dihidrolisis menjadi asam amino yang kemudian digunakan dalam proses translasi untuk menginterpretasi suatu pesan genetik dan membentuk protein dalam proses awal perkecambahan. Awal perkecambahan adalah tahapan pertama dari proses perkecambahan dimana perubahan biokimia telah banyak terjadi. Tujuan dari penelitian ini adalah mengetahui karakteristik perubahan kandungan protein terlarut dan pola protein biji tanaman melinjo pada awal perkecambahan. Penelitian ini menggunakan 2 varietas biji melinjo yaitu varietas Gentong dan Kerikil dengan tiga tahap perlakuan yaitu biji awal, biji setelah perlakuan stratifikasi hangat selama 3 minggu dan biji setelah 1 bulan semai. Sampel biji melinjo diekstrak untuk menentukan kandungan protein terlarut dan pola protein menggunakan 15% SDS-PAGE. Hasil penelitian menunjukkan bahwa kandungan protein terlarut mengalami perubahan yang berbeda pada setiap tahap perlakuan. Analisa pola protein menunjukkan adanya 2 jenis pita protein yang mengalami perubahan pada awal perkecambahan yaitu protein dengan berat molekul 66 kDa dan 14 kDa. Kata Kunci: Biji Melinjo; Awal Perkecambahan; Protein; SDS-PAGE How to citate: Siregar AS, TA Siswoyo, B Sukowardojo. 2013. Karakteristik perubahan protein biji melinjo (Gnetum gnemon) pada awal perkecambahan. Berkala Ilmiah Pertanian 1(2): 24-26.
CHANGES OF SUCROSE CONTENT AND INVERTASE ACTIVITY DURING SUGARCANE STEM STORAGE Siswoyo, Tri Agus; Oktavianawati, Ika; ., Djenal; Sugiharto, Bambang; Murdiyanto, Untung
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

ABSTRACTInvertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the keyenzymes involved in sucrose metabolism in sugarcane plants.They are highly correlated with sucrose and reducing sugar contentsduring plant growth. The sugarcane plants have two kindsof invertases, namely neutral invertase (NI) and acid invertase(AI). They have different function in sucrose accumulation.The research aimed to study the role of AI and NI in accumulationof reducing sugar during storage of sugarcane stems. Plantmaterials of 18-month-old field grown sugarcane of the commercialvariety R-579 (wet-land) and M 442-51 (dry-land) wereused. Three internodes were sampled to represent immature(internode 1-8/F1), maturing (internode 9-16/F2), and mature(internode 17-24/F3) stem tissues. All tissues were stored for0-9 days at room temperature (28-32oC) and each day, the samplewas extracted to determine invertase activity, total solubleprotein, and sugar contents. This observation was valid forinvertase activity expressed on a protein basis. At the initiateharvested (0-3 days), NI had a higher specific activity than AIin the sucrose-accumulating region of the sugarcane stems.Negative significant correlation was found between NI specificactivity and sucrose accumulation (r2 = 0.41, P < 0.05). AIshowed a higher specific activity after 4 days harvested and hadnegative correlation with sucrose accumulation (r2 = 0.40, P <0.05). These results showed that NI could be more responsiblein sucrose hydrolisis than AI at early storage of sugarcane stems.
Isolation and Characterization of the Expression of Gene for Sucrose Transporter Proteins in Sugarcane Plant (Saccharum officinarum) Harianti Novita; Tri Agus Siswoyo; Bambang Sugiharto
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Sucrose as the major transported form of fixed carbon must be translocated from source tissue to the sites of consumption and storage or sink tissues. The translocation of sucrose is facilitated by some distinct sucrose transporters proteins (SUT). To study sucrose transporters in sugarcane, we had conducted isolation and characterization of gene encoding sucrose transporter protein. The isolation was performed with RT-PCR method using total RNA isolated from sugarcane leaf and primer designed from conservative region of SUTcDNAs of SoSUT2A (accession number AY65599), OsSUT-1(accession number AAP54842, OsSUT-1 mRNA (accession number XM 46477). Based on the conservative amino acids sequences of QILQQFA and MGKTEPV, the corresponding sequences of the primers for RT-PCR were: P1, (forward) 5’CAGATCCTTCAACAGTTCGC-3’ and P2 (reverse) 5’-TGCCCTTTGTCTCCGGAACC-3’, respectively. Agarose gel electrophoresis shown a clear single 0.5 kb cDNA band of the PCR product. Thus, the DNA was cloned into pGEMT vector (Promega) for further analysis. Sequence determination of the PCR product revealed a nucleotides sequence of 543 bp in length and has a high homology around 89%, 87.3 % and 84.8 % with maize ZmSUT-1, sugarcane SoSUT 2A and rice OsSUT-1 mRNA, respectively. We designated the cDNA as SoSUT2 and the nucleotide sequence have been submitted to GenBank data base under accession number bankit 734628. By using PSORT analysis the fragment of cDNA-SoSUT2 encoded protein may be located in the endoplasmic reticulum. To have a better understanding, the expression of SoSUT2 gene in sugarcane was determinate by RT-PCR method using total RNA isolated from leaf, petioles, stem and root and visualized the PCR product in agarose gel electrophoresis. Based on the cDNA bands intensity, it can be illustrated that the expression of SoSUT2 gene were found highest in sugarcane leafs then petioles and stem, but the expression was not found in root. Although the SoSUT2-cDNA has not been isolated in full size, the results suggest the presence of gene family of SUT in sugarcane.
AKTIVITAS DAN STABILITAS RADICAL SCAVENGING L-ASKORBIL PALMITAT HASIL SINTESIS SECARA ENZIMATIK [Activity and Stability of Radical Scavenging of L- Palmitate Synthesized Enzymatically] Tri Agus Siswoyo; Tri Ardiyati
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

L-ascorbyl palmitate (AsA-Pal-Enz) was synthesized by using an immobilized lipase from Aspergillus niger. A comparison of antioxidative effects between L-ascorbic acid  (AsA) and AsA-Pal-Enz was determined in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging. The results indicate that the AsA-Pal-Enz was effective in preventing lipid oxidation, while the antioxidative activity in authentic AsA-Pal was lower. The activity of AsA-Pal-Enz was very stable than AsA-Pal standard during heating. 
PENGARUH KONSENTRASI DAN WAKTU APLIKASI CaCl2 TERHADAP FISIKOKIMIA BUAH TOMAT (Lycopersicum esculentum Mill.) Lina Asri Wulandari; Tri Agus Siswoyo; Kacung Hariyono
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) Jurnal Bioindustri: VOL. 2 NO. 1 (2019)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v2i1.481

Abstract

Tomato was classified as a climacteric fruit with a fast ripening period andthen followed by the increase of fruit softening. The inhibition of fruit softening was needed to maintain the quality and shelf life of the fruit. One of the way to inhibit the tomatoes softening was calcium application. However, the best concentration and timing of calcium application for tomato was still limited. The aimed of research was to determine the best combination of concentration and time of application of calcium chloride (CaCl2) to the physicochemical of tomato. Experiment was arranged in factorial completely randomized design (CRD) with two factors and three replications. First factor was concentration of CaCl2 which consisted of 5 levels (0 M; 0.3 M; 0.6 M; 0.9 M; 1.2 M), while the second factor was application time of CaCl2 (pre- and post-harvesting). The results showed that there was no interaction between concentration and application time of CaCl2 to the physicochemical of tomatoes. Treatment of CaCl2 1.2 M could increased fruit calcium content, inhibited weight loss and fruit softness, increased total titrated acid content, and inhibited the degradation of vitamin C. The application time of CaCl2 in pre- and post-harvest was not significantly different in term of physicochemical properties. Keywords: fruit softness, quality, weight loss, shelf life, vitamin C
Perubahan Kandungan Sukrosa dan Aktivitas Invertase pada Batang Tebu Selama Pemanenan Tri Agus Siswoyo; Ika Oktavianawati; Bambang Sugiharto; Untung Murdiyanto
Zuriat Vol 17, No 2 (2006)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v17i2.6739

Abstract

Invertase (_-D-fructofuranosidase, E.C. 3.2.1.26) merupakan enzim kunci dalam metabolisme sukrosa pada tanaman tebu serta berkolerasi tinggi terhadap kandungan sukrosa dan gula reduksi selama masa pertumbuhan. Pada tanaman tebu terdapat 2 jenis invertase yaitu Acid Invertase (AI) dan Neutral Invertase (NI) yang mempunyai fungsi berlainan dalam pengakumulasion sukrosa. Penelitian ini bertujuan mempelajari peranan AI dan NI setelah pemanenan serta hubungannya terhadap perubahan kandungan sukrosa. Hasil penelitian menunjukkan aktivitas NI tertinggi terjadi pada awal pemanenan dan mempunyai korelasi negatif yang nyata sebesar (r2) = 0.41 dengan kandungan sukrosa. Aktivitas AI baru menunjukkan aktivitas yang meningkat setelah umur penyimpanan diperlama dan mempunyai korelasi negatif yang tinggi dengan kandungan sukrosa sebesar (r2) = 0.40. Dari hasil tersebut dapat dikatakan bahwa peranan NI lebih menentukan terhadap degradasi sukrosa pada awal pemanenan.
Effect of Sodium Chloride on Thermal Properties of 30 kDa Protein Isolated from Melinjo Seed Tri Agus Siswoyo
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 3 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The thermal properties of melinjo (Gnetum gnemon) protein were studied using differential scanning calorimetry. The melinjo protein obtained from crude seed protein was isolated using isoelectric precipitation method. The molecular weight of the protein isolate about 30 kDa as estimated using SDS-polyacrylamide gel electrophoresis. The thermogram of MSPI showed a major endothermic peak at 84.10C and a minor transition at 109.50C with enthalpy value of 0.52 and 0.32 J/g, respectively. The progressive increasing in major endothermic peak with increase in NaCl concentrations, which suggested a more compact conformation of MSPI with higher thermal stability. Treatment by heating the sample at 800C for 5 min caused reduction of enthalpy value, indicated that MSPI denaturation occurred. In contrast, after MSPI combined with NaCl, the enthalpy value was increased, which showed that the of MSPI was more heat stable. Key words: Melinjo, protein isolated, thermal, NaCl, enthalpy
Screening Fitokimia dan Studi Aktivitas Ekstrak Daun Sintok (Cinnamomum sintoc Bl.) Sebagai Antioksidan dan Antihiperlipidemia Kumalasari, Ardine; Handayani, Wuryanti; Siswoyo, Tri Agus
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9916

Abstract

Cinnamomum sintoc Bl. merupakan tanaman yang memiliki kandungan minyak atsiri di kulit batangnya yang digunakan untuk antioksidan, antihiperlipidemia dan lain-lain. Dari penelitian ini, senyawa metabolit sekunder dari daun sintok (kadar air 54,7%±0,69) diekstrak dengan metanol dan dianalisis dengan reagen spesifik. Daun sintok diekstraksi secara maserasi bertingkat dengan meningkatkan kepolaran pelarut, yaitu n-heksana, etil asetat, metanol. Uji antioksidan dan antihiperlipidemia dilakukan pada setiap ekstrak(HS, ES, MS) menggunakan standar asam galat equivalent. Total fenolik dan total flavonoid dihitung menggunakan kurva standar asam galat dan kuersetin, hasil total fenolik dari setiap ekstrak antara lain HS (39,23±2,79 mg AGE/g); ES (110,77±2,37 mg AGE/g); dan MS (283,63±3,96 mg AGE/g). Aktivitas antioksidan pada ekstrak ditentukan dengan kemampuan ekstrak untuk meredam DPPH sedangkan aktivitas antihiperlipidemia ekstrak ditentukan dengan kemampuan ekstrak untuk menghambat kinerja lipase. Potensi ekstrak daun sintok terhadap antioksidan cukup tinggi hanya untuk ekstrak MS, sedangkan potensi terhadap antihiperlipidemia untuk semua jenis ekstrak. Kata Kunci: Cinnamomum sintoc Bl., fitokimia, maserasi, fenolik, antioksidan, antihiperlipidemia.
AKTIVITAS LIPASE DAN METABOLISME LIPID SELAMA MASA PERKECAMBAHAN WIJEN (Sesamum indicum. L) Tri Agus Siswoyo; Norry Eka Palupi
JURNAL AGROTEKNOLOGI Vol 1 No 02 (2007)
Publisher : Faculty of Agricultural Technology, University of Jember

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Abstract

Experiments were conducted to determine the relationship between lipase activity and the metabolism of lipids during germination of sesame (Sesamum indicum. L) seed. During the course of germination, concentration of the non-polar lipid fractions and lipase activity increased. The glycolipids and phospholipid fractions were relative stable during early germination, but there were marked changes phospholipid fractions after 5 days germination. In a comparison among lipid fractions Non-polar lipids (NL), Glycolipids (GL), and Phospholipids (PL), there were no significant regressions between the quantitative changes in lipid fractions and lipase activities. It was concluded that the degradation in NL and increase in PL and GL observed during sesame germination were not controlled by the level of lipase activity. Rather, it was suggested that sesame may have another lipase present in the cells where the oil bodies are located that hydrolyzes lipids from the oil bodies during germination. Keywords: lipase, glycolipids, phospholipid, lipase, sesame seed
TEKNOLOGI PEMANFAATAN LIMBAH PADAT PENGOLAHAN KERIPIK SINGKONG MENJADI PAKAN PELET AYAM PEDAGING DI DESA BARATAN KABUPATEN JEMBER Listya Purnamasari; Nurul Pratiwi; Tri Agus Siswoyo
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v2i1.687

Abstract

      Baratan Village, Patrang Sub-district, Jember District has a high potential production of cassava chips by Home Industry (IRT). Solid waste of cassava (cassava husk) from cassava processing into cassava chips has not been utilized properly. Cassava husk can be used as raw material for animal feed. The purpose of this program is the empowerment of the community of Baratan IRT in the processing of cassava husk into broiler feed and to increase the value of the waste and can improve the economic of the IRT in Baratan Village. Cassava husk has a deficiency because of the antinutrient content (Cyanide Acid) and dangerous when consumed. The methods used in this program are counseling and chipping of cassava with chipping tools, counseling and training of utilitation of cassava waste, counseling and training of broiler feed formulation, pelet making, mentoring, monitoring and evaluation. The result of this program is the IRT Baratan Village Jember Regency is able to process the solid waste from processing of cassava chips into broiler feed (pelet). Guidebooks can be used by IRT well so they can treat the waste into feed independently. IRT cassava chips as a partner of this program can implement and apply the technology that has been given and strive to always continuous in the manufacture of feed and pellet product development in an effort to improve the welfare and sustainability of the program.Keywords: cassava waste, pelet feed, Baratan Village