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All Journal Jurnal Teknologi Dan Industri Pangan Zuriat Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Berkala Ilmiah Pertanian BERKALA SAINSTEK Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science UNEJ e-Proceeding Jurnal Bioteknologi & Biosains Indonesia (JBBI) Journal of Livestock Science and Production Indonesian Journal of Chemistry Jurnal Bioindustri (Journal of Bioindustry)
Tri Agus Siswoyo
Program Studi Magister Bioteknologi, Universitas Jember, Jl. Kalimantan, Kampus Tegal Boto, Jember 68121, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Mathematics Physics Veterinary

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KARAKTERISTIK PERUBAHAN PROTEIN BIJI MELINJO (GNETUM GNEMON) PADA AWAL PERKECAMBAHAN Siregar, Adrian Syawaluddin; Siswoyo, Tri Agus; Sukowardojo, Bambang
Berkala Ilmiah Pertanian (e-ISSN: 2338-8331) Vol 1, No 2: NOVEMBER
Publisher : Berkala Ilmiah Pertanian (e-ISSN: 2338-8331)

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Abstract

[ENGLISH] Protein was one of macromolecule in seed which played an important role in the process of germination. Storage protein in seed was hydrolyzed to amino acid which are then used in the translation process for a genetic message interpretating and protein building in pre-germination process. Pre-Germination was the first step of germination that have many biochemical changes. The objectives of this research was to know biochemical characteristic of soluble protein and protein pattern of melinjo seed during pre-germination. Two varieties of melinjo seed Gentong and Kerikil was used in this research with three stages of treatment (initial seed, 3 weeks warm stratification seed and 1 month seed seedling). Seed samples are extracted to determine total soluble protein and protein pattern with 15 % SDS-PAGE. The results showed that total soluble protein have a change different at each stage of the treatment. Protein patterns analysis indicates band protein with molecular weight 66 kDa and 14 kDa change during pre-germination. Keywords: Melinjo Seed; Pre-Germination; Protein; SDS-PAGE [INDONESIAN] Protein merupakan salah satu makromolekul dalam biji yang berperan penting pada proses perkecambahan. Protein yang tersimpan pada biji dihidrolisis menjadi asam amino yang kemudian digunakan dalam proses translasi untuk menginterpretasi suatu pesan genetik dan membentuk protein dalam proses awal perkecambahan. Awal perkecambahan adalah tahapan pertama dari proses perkecambahan dimana perubahan biokimia telah banyak terjadi. Tujuan dari penelitian ini adalah mengetahui karakteristik perubahan kandungan protein terlarut dan pola protein biji tanaman melinjo pada awal perkecambahan. Penelitian ini menggunakan 2 varietas biji melinjo yaitu varietas Gentong dan Kerikil dengan tiga tahap perlakuan yaitu biji awal, biji setelah perlakuan stratifikasi hangat selama 3 minggu dan biji setelah 1 bulan semai. Sampel biji melinjo diekstrak untuk menentukan kandungan protein terlarut dan pola protein menggunakan 15% SDS-PAGE. Hasil penelitian menunjukkan bahwa kandungan protein terlarut mengalami perubahan yang berbeda pada setiap tahap perlakuan. Analisa pola protein menunjukkan adanya 2 jenis pita protein yang mengalami perubahan pada awal perkecambahan yaitu protein dengan berat molekul 66 kDa dan 14 kDa. Kata Kunci: Biji Melinjo; Awal Perkecambahan; Protein; SDS-PAGE How to citate: Siregar AS, TA Siswoyo, B Sukowardojo. 2013. Karakteristik perubahan protein biji melinjo (Gnetum gnemon) pada awal perkecambahan. Berkala Ilmiah Pertanian 1(2): 24-26.
CHANGES OF SUCROSE CONTENT AND INVERTASE ACTIVITY DURING SUGARCANE STEM STORAGE Siswoyo, Tri Agus; Oktavianawati, Ika; ., Djenal; Sugiharto, Bambang; Murdiyanto, Untung
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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ABSTRACTInvertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the keyenzymes involved in sucrose metabolism in sugarcane plants.They are highly correlated with sucrose and reducing sugar contentsduring plant growth. The sugarcane plants have two kindsof invertases, namely neutral invertase (NI) and acid invertase(AI). They have different function in sucrose accumulation.The research aimed to study the role of AI and NI in accumulationof reducing sugar during storage of sugarcane stems. Plantmaterials of 18-month-old field grown sugarcane of the commercialvariety R-579 (wet-land) and M 442-51 (dry-land) wereused. Three internodes were sampled to represent immature(internode 1-8/F1), maturing (internode 9-16/F2), and mature(internode 17-24/F3) stem tissues. All tissues were stored for0-9 days at room temperature (28-32oC) and each day, the samplewas extracted to determine invertase activity, total solubleprotein, and sugar contents. This observation was valid forinvertase activity expressed on a protein basis. At the initiateharvested (0-3 days), NI had a higher specific activity than AIin the sucrose-accumulating region of the sugarcane stems.Negative significant correlation was found between NI specificactivity and sucrose accumulation (r2 = 0.41, P < 0.05). AIshowed a higher specific activity after 4 days harvested and hadnegative correlation with sucrose accumulation (r2 = 0.40, P <0.05). These results showed that NI could be more responsiblein sucrose hydrolisis than AI at early storage of sugarcane stems.
Screening Fitokimia dan Studi Aktivitas Ekstrak Daun Sintok (Cinnamomum sintoc Bl.) Sebagai Antioksidan dan Antihiperlipidemia Kumalasari, Ardine; Handayani, Wuryanti; Siswoyo, Tri Agus
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9916

Abstract

Cinnamomum sintoc Bl. merupakan tanaman yang memiliki kandungan minyak atsiri di kulit batangnya yang digunakan untuk antioksidan, antihiperlipidemia dan lain-lain. Dari penelitian ini, senyawa metabolit sekunder dari daun sintok (kadar air 54,7%±0,69) diekstrak dengan metanol dan dianalisis dengan reagen spesifik. Daun sintok diekstraksi secara maserasi bertingkat dengan meningkatkan kepolaran pelarut, yaitu n-heksana, etil asetat, metanol. Uji antioksidan dan antihiperlipidemia dilakukan pada setiap ekstrak(HS, ES, MS) menggunakan standar asam galat equivalent. Total fenolik dan total flavonoid dihitung menggunakan kurva standar asam galat dan kuersetin, hasil total fenolik dari setiap ekstrak antara lain HS (39,23±2,79 mg AGE/g); ES (110,77±2,37 mg AGE/g); dan MS (283,63±3,96 mg AGE/g). Aktivitas antioksidan pada ekstrak ditentukan dengan kemampuan ekstrak untuk meredam DPPH sedangkan aktivitas antihiperlipidemia ekstrak ditentukan dengan kemampuan ekstrak untuk menghambat kinerja lipase. Potensi ekstrak daun sintok terhadap antioksidan cukup tinggi hanya untuk ekstrak MS, sedangkan potensi terhadap antihiperlipidemia untuk semua jenis ekstrak. Kata Kunci: Cinnamomum sintoc Bl., fitokimia, maserasi, fenolik, antioksidan, antihiperlipidemia.
Effect of Sodium Chloride on Thermal Properties of 30 kDa Protein Isolated from Melinjo Seed Tri Agus Siswoyo
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 3 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The thermal properties of melinjo (Gnetum gnemon) protein were studied using differential scanning calorimetry. The melinjo protein obtained from crude seed protein was isolated using isoelectric precipitation method. The molecular weight of the protein isolate about 30 kDa as estimated using SDS-polyacrylamide gel electrophoresis. The thermogram of MSPI showed a major endothermic peak at 84.10C and a minor transition at 109.50C with enthalpy value of 0.52 and 0.32 J/g, respectively. The progressive increasing in major endothermic peak with increase in NaCl concentrations, which suggested a more compact conformation of MSPI with higher thermal stability. Treatment by heating the sample at 800C for 5 min caused reduction of enthalpy value, indicated that MSPI denaturation occurred. In contrast, after MSPI combined with NaCl, the enthalpy value was increased, which showed that the of MSPI was more heat stable. Key words: Melinjo, protein isolated, thermal, NaCl, enthalpy
Effects of Different Lipid Extractions on Gelatinization and Retrogradation of Bitter Nuts (Gnetum gnemon) Starch Tri Agus Siswoyo
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 2 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The effect of different lipid extraction on gelatinization and retrogradation of bitter nuts (Gnetum gnemon) starch was studied by the measurement of starch-lipids complex formation using differential scanning calorimetry. The bitter nuts samples were extracted sequentially with hexane for surface lipid starch (SL) and hot water-saturated butanol for internal lipid starch (IL). The gelatinization enthalpies of starch increased significantly with extracting step, but the starch-lipid complex enthalpies of SL and IL were statistically lower, when compared with the native starch. According to the Avrami equation, the retrogradation rate of native bitter nuts starch was slower than that of the SL or IL, whereas the retrogradation rate of IL was slower than SL. High number of starch-lipids complex could retard the retrogradation of bitter nuts starch during storage. Key words : Gnetum gnemon, bitter nuts, gelatinization, retrogradation, avrami equation, starch-lipid complex.
AKTIVITAS DAN STABILITAS RADICAL SCAVENGING L-ASKORBIL PALMITAT HASIL SINTESIS SECARA ENZIMATIK [Activity and Stability of Radical Scavenging of L- Palmitate Synthesized Enzymatically] Tri Agus Siswoyo; Tri Ardiyati
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

L-ascorbyl palmitate (AsA-Pal-Enz) was synthesized by using an immobilized lipase from Aspergillus niger. A comparison of antioxidative effects between L-ascorbic acid  (AsA) and AsA-Pal-Enz was determined in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging. The results indicate that the AsA-Pal-Enz was effective in preventing lipid oxidation, while the antioxidative activity in authentic AsA-Pal was lower. The activity of AsA-Pal-Enz was very stable than AsA-Pal standard during heating. 
Perubahan Kandungan Sukrosa dan Aktivitas Invertase pada Batang Tebu Selama Pemanenan Tri Agus Siswoyo; Ika Oktavianawati; Bambang Sugiharto; Untung Murdiyanto
Zuriat Vol 17, No 2 (2006)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v17i2.6739

Abstract

Invertase (_-D-fructofuranosidase, E.C. 3.2.1.26) merupakan enzim kunci dalam metabolisme sukrosa pada tanaman tebu serta berkolerasi tinggi terhadap kandungan sukrosa dan gula reduksi selama masa pertumbuhan. Pada tanaman tebu terdapat 2 jenis invertase yaitu Acid Invertase (AI) dan Neutral Invertase (NI) yang mempunyai fungsi berlainan dalam pengakumulasion sukrosa. Penelitian ini bertujuan mempelajari peranan AI dan NI setelah pemanenan serta hubungannya terhadap perubahan kandungan sukrosa. Hasil penelitian menunjukkan aktivitas NI tertinggi terjadi pada awal pemanenan dan mempunyai korelasi negatif yang nyata sebesar (r2) = 0.41 dengan kandungan sukrosa. Aktivitas AI baru menunjukkan aktivitas yang meningkat setelah umur penyimpanan diperlama dan mempunyai korelasi negatif yang tinggi dengan kandungan sukrosa sebesar (r2) = 0.40. Dari hasil tersebut dapat dikatakan bahwa peranan NI lebih menentukan terhadap degradasi sukrosa pada awal pemanenan.
Analisis In Vivo Aktivitas Antihipertensi dari Protein Biji Melinjo (Gnetum gnemon) Terhidrolisis Yeni Tri Puspitaningrum; Erfan Efendi; Tri Agus Siswoyo
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Hypertension is a serious health problem and requires a good handling. An alternative treatment for hypertension is required due to the treatment of hypertension took a long period of time causing unwanted side effects. Melinjo seed hydrolyzed-proteins have an ACE-inhibitor activity, an antihypertensive effect. This study aimed to determine the antihypertensive effects of melinjo seed hydrolyzed-proteins in hypertensive rats. Wistar rats induced with prednisone 1,5 mg/kg body weight and 2% NaCl for 7 days and then treated. There were 7 groups: a normal group K, a group K(-) which is a hypertensive rats without treatment, a group K(+) which is hypertensive rats with captopril 2,5 mg/kg body weight on day 8th, and four treatment groups P1, P2, P3, and P4 which are hypertensive rats with melinjo seed hydrolyzed-proteins with a dose of 5, 10, 20, and 30 mg/kg body weight on day 8th, respectively. Blood pressure was measured before and after treatment every 1 hour for 5 hours. The results showed that melinjo seed (Gnetum gnemon) hydrolyzed-proteins can decrease blood pressure in hypertensive rats.   Keywords: Antihypertensive, Gnetum gnemon, Hydrolized-Protein, Blood Pressure
Aktivitas Antioksidan dan Kemampuan Proteksi terhadap Kerusakan DNA dari Protein Isolat Biji Melinjo (Gnetum Gnemon L.) Terhidrolisis Menggunakan Alkalase Terimobilisasi (Antioxidant Activity and Protection Ability Against DNA Damage of Isolate Protein Me Anandini Aulia Safiera; Endah Puspitasari; Tri Agus Siswoyo
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i2.5870

Abstract

Hydrolyzed protein isolates of melinjo seed using free alcalase has been known to have antioxidant potential. However, free alcalase can only be used once. Enzymes immobilization technique with entrapment method would overcome this problem. The immobilized enzyme characteristic was observed by SEM and hydrolysis degree value. The success of hydrolysis process was observed by SDS-PAGE. Antioxidant activity test was determined by the ability to inhibit hydroxyl radical and protection against DNA damage in 1% agarose gel. The result showed that protein isolates from melinjo seed had been successfully hydrolyzed by immobilized alcalase. Following hydrolysis showed that immobilized alcalase was effective to hydrolyze protein up to four times. Based on hydroxyl radical reduction activity test, antioxidant activity of hydrolyzed protein using immobilized alcalase had the same as that of glutathione as positive control antioxidant activity. In addition, hydrolyzed protein using immobilized alcalase had the ability toprotect DNA damage which showed by reversible change of supercoil band. Keywords: melinjo seed, hydrolyzed protein, immobilized alcalase, antioxidant
Hidrolisis Protein Isolat Biji Melinjo (Gnetum gnemon L.) menggunakan Alkalase Terimobilisasi dan Aktivitasnya sebagai Antihipertensi (Hydrolysis of Melinjo Seed (Gnetum gnemon L.) Isolated-Protein using Immobilized Alcalase and Its Activity as Antihyper Nur Fauziah Matra; Endah Puspitasari; Tri Agus Siswoyo
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.6612

Abstract

Hypertension is ranked third on the cause of death in Indonesia for all ages (6.8 %). Protein isolate of melinjo seeds (Gnetum gnemon L.) hydrolyzed with free alcalase has been known to have antihypertensive activity. However, the use of free alcalase is uneconomical since it can only be used once, thus we studied the immobilized alcalase effectivity to hydrolise the melinjo seeds protein isolate to be used as angiotensin converting enzyme (ACE) inhibitor. The immobilization method used entrapment method with DMDMOS/TMOS as matrix. Characterization of the immobilized enzyme was observed by FTIR and effectiveness of repeated hydrolysis was observed by the value of the degree of hydrolysis. The success of the hydrolysis process was determined by SDS-PAGE electrophoresis. Antihypertensive activity test was determined by the ability to inhibit ACE. The results showed that the alcalase has been immobilized in the matrix of silane and SDS-PAGE protein profile showed melinjo seed proteins had been successfully hydrolyzed. Hydrolysis process repeatedly demonstrated that immobilized alcalase was effective to hydrolyze protein isolates of melinjo seeds twice. Based on ACE inhibitory test, there were no significant differences between the protein isolate hydrolyzed by immobilized alcalase (Gg-PH), protein isolates hydrolyzed with free alcalase (Gg-PHB), and captopril. These findings suggest that the antihypertensive activity in Gg-PH is the same with that of captopril. Keywords: hypertension, antihypertensive, melinjo, enzymes immobilization, ACE inhibitor
Co-Authors A.A. Istri Ratnadewi Adrian Syawaluddin Siregar Adrian Syawaluddin Siregar Ali Santosa Anandini Aulia Safiera Aprila Iga Mufidah Arbi Bahtiar Boedi Iman Halanobis Ardine Kumalasari Bambang Kusmanadhi Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sukowardojo Bambang Sukowardojo Budipratiwi Wisudyaningsih Budipratiwi Wisudyaningsih Djenal . Djenal Djenal Dwi Setyawan Endah Puspitasari Erfan Efendi Fariza Oktaviani Fitra Dea Wafa Frida Oktaningtias Widiarthi Harianti Novita Ika Oktavianawati Irma Novita Sari Jainur Rochman Kacung Hariyono Kartika Tari Azhar Kumalasari, Ardine Lina Asri Wulandari listya purnamasari Martiyas Pujirahayu Mohammad Ubaidillah Nabila Nur Aisyah Al Ayyubi Norry Eka Palupi Nur Fauziah Matra Nurul Pratiwi Rahmawati Rahmawati Restanto, Didik Pudji Reza Septyan Pratama Sholeh Avivi Sigit Soeparjono Siswandono, Siswandono Sri Hartatik Tri Ardiyati TRI HANDOYO Untung Murdiyanto Untung Murdiyanto Untung Murdiyanto Wuryanti Handayani Yagus Wijayanto Yeni Tri Puspitaningrum Yudi Wicaksono