Journal of Fisheries & Marine
Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE [Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]

Gunanti Mahasri (Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.)
Ulia Fajriah (Program Studi Akuakultur, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.)
Sri Subekti (Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.)

Article Info

Publish Date
01 Apr 2010

Abstract

Abstract One of the diseases that often attack are fish is parasitic disease caused by Lernaea cyprinacea, called Lernaeosis. This ectoparasites could be detected on skin, gill, eyes, fin or inside of mouth and nostril of fishes. The aim of the study was to know the character is tic of L.cyprinacea protein based on molecular weight using SDS-PAGE. The results of this study were expected as Lernaeosis vaccine candidate. The experiment used SDS-PAGE with 12% separating gel, 3% stacking gel, voltage 110 volts 28 A and gel staining using coomassie blue. The result showed that, there were eight protein bands with molecular weight 82.3, 73.3, 66.6, 60.5, 54.9, 27.5, 23.1 and 19.8 kDa. Among them, the bands with molecular weight 27.5, 23.1 and 19.8 kDa were thicker and clearer than the others.

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Journal Info
Journal of Fisheries & Marine
Website

Abbrev

JIPK

Publisher

Universitas Airlangga

Subject

Biochemistry, Genetics & Molecular Biology

Description

Jurnal Ilmiah Perikanan dan Kelautan (JIPK; English: Scientific Journal of Fisheries and Marine) ISSN International Centre | ISSN:2528-0759 (Online) | ISSN: 2085-5842 (Print) JIPK is a peer-reviewed and open access biannually (April and November) that published by the Faculty of Fisheries and ...