Folia Medica Indonesiana
Vol. 54 No. 1 (2018): March

Effects of Arak Bali Administration on Spermatozoa DNA Fragmentation and Testosterone Level of Rats (Rattus norvegicus)

Alfiah Hayati (Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya)
Dwi Winarni (Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya)
Ni Wayan Sukma Antari (Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya)
Inne Ninda Resita (Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya)
Erlyn Nurul Fauziah (Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya)



Article Info

Publish Date
05 Apr 2018

Abstract

This study aimed to determine the effects of arak bali on the fragmentation of spermatozoa and testosterone in rats (Rattus norvegicus). This study used 24 rats (170-200 g), divided into four groups: one control and three treatments (receiving arak bali containing 40% alcohol as much as 0.1 and 0.5 mL and synthetic alcohol (40%) as much as 0.1 mL, for 45 days). The observation of DNA fragmentation was done using acridine orange staining and the measurement of testosterone level used ELISA method. The results showed that the provision of arak bali in experimental animals increased the occurrence of spermatozoa DNA fragmentation. The higher the volume of arak bali given, the higher the fragmentation of spermatozoa DNA. The administration of arak bali also decreased testosterone level. The higher the volume of arak bali given, the lower the rats' testosterone level.

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Journal Info

Abbrev

FMI

Publisher

Subject

Other

Description

Folia Medica Indonesiana publishes articles in the field of medical science in particular and health sciences in general, as well as a variety of other fields related to those two disciplines. Most of the articles are research article, and others are articles on case reports and literature review. ...