JSFK (Jurnal Sains Farmasi & Klinis)
Vol 3, No 1 (2016): J Sains Farm Klin 3(1), November 2016

Identifikasi Type Human Papillomavirus (HPV) pada Penderita Kanker Serviks

Marlina Marlina (Fakultas Farmasi Universitas Andalas)
Yufri Aldi (Fakultas Farmasi Universitas Andalas)
Andani Eka Putra (Fakultas Farmasi Universitas Andalas)
Densi Selpia Sopianti (Fakultas Farmasi Universitas Andalas)
Dewi Gulyla Hari (Fakultas Farmasi Universitas Andalas)
Arfiandi Arfiandi (Fakultas Farmasi Universitas Andalas)
Akmal Djamaan (Fakultas Farmasi Universitas Andalas)
Rustini Rustini (Fakultas Farmasi Universitas Andalas)



Article Info

Publish Date
28 Dec 2016

Abstract

Human Papillomavirus (HPV) is the most significant risk factor for the cause of cervical cancer. The purpose of this study for identification of HPV types 16, 18, 31, 33, 45 and 52 in cervical cancer patients. HPV is a row of high-risk HPV types that can cause cervical cancer. Total sample of 78 diisolat DNA derived from FFPE, cervical smears and cervical cancer fresh tissue obtained from Dr. Dr. M. Djamil, Padang and hospitals. Arifin Achmad, Pekanbaru. HPV DNA detection is done by using Polymerase Chain Reaction (PCR) using universal primers GP5 +/6 +. HPV types were identified by PCR with specific primers. Total sample types obtained with concentrations varying between 0.9 to 645 ng / ml with purity DNA in accordance with the specified purity for PCR amplification. The results of the study of 78 patients with cervical cancer samples, 42 samples (54%) identified HPV DNA. HPV type 18 is more dominant and followed by HPV type 16 as compared to the other types, namely the percentage of 40.4% and 28.5%. HPV type 45 (7.1%), HPV type 52 (2.3%) and HPV 31 and HPV type 33 was not detected.

Copyrights © 2016






Journal Info

Abbrev

jsfk

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Description

Jurnal Sains Farmasi & Klinis (J Sains Farm Klin) adalah jurnal ilmiah di bidang kefarmasian yang diterbitkan oleh Fakultas Farmasi Universitas Andalas bekerjasama dengan lembaga profesi "Ikatan Apoteker Indonesia" (IAI) daerah Sumatera Barat sejak tahun ...