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The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Zam, Syukria Ikhsan; Agustien, Anthoni; Syamsuardi, Syamsuardi; Djamaan, Akmal; Mustafa, Irfan
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
Kajian Biodegradasi Filem Plastik Campuran Polistiren dengan Poli(3hidroksibutirat-ko-3-hidroksivalerat) dalam Tanah Secara In-vitro Permatadewi, Asiska; Zaini, Erizal; Rustini, Rustini; Djamaan, Akmal
Jurnal Farmasi Andalas Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Andalas

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Abstract

The in vitro biodegradation testing of plastic of the mixture of polystyrene (PS) and poly (3-hydroxybutirate-co-3-hydroxyvalerat) [P(3HB-ko-3HV)] in soil has been carried out. Plastic film was produced by using blending technique and followed by solved casting at various composition. The biodegradation testing was conduted by using soil burial method. Rseult showed that the increasing of component [P(3HB-ko-3HV)] in the mixture of plastic film was also increase the biodegradation rate of plastic film in the soil. At the composition 100:0, 95:5, 90:10 and 85:15 of plastic film in 5 weeks burial was found weigh remaining of plastic were of 99.67; 95.71; 95.03; 94.13% w/w respectively. It is also observed that the amount of bacteria in the soil were of 2.57 x 106 colonies/g.
Kajian Biodegradasi Filem Plastik Campuran Polistiren Dengan Poli (3-Hidroksibutirat) Dalam Tanah Octaviani, Melzi; Zaini, Erizal; Djamaan, Akmal
Jurnal Farmasi Andalas Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Andalas

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Abstract

The degradation of a plastic film containing polymer synthetic polystyrene (PS) and biopolymer poly(3-hydroxybutyrate) [P(3HB)] in soil has been carried out based on soil burial test. Result showed that the amount of P(3HB) in plastic film containing PS and P(3HB) influence the degradation rate of plastic film. Rate degradation measured use linear regression equality and parameter is indicated by the slope (k), degradation time 50% (t 50%), and degradation time 95% (t 95%). Degradation plastic film which occurred in soil with ratio PS/P(3HB) 85:15 was k = 0.90 %/week, t 50% = 55.13 weeks and t 95% = 105.29 weeks, followed by PS/P(3HB) 90:10 and PS/P(3HB) 95:15. In contrast, plastic film which made from pure PS (100:0) occurred slow with slope value (k) = 0.14 %/week, t 50% = 345.07 weeks, and t 95% = 655.63 weeks. Result of SEM test showed that damage occurred and erosion at surface of plastic film as long as observation time in various environment condition. 
UJI BIODEGRADASI FILEM PLASTIK CAMPURAN POLISTIREN DENGAN POLI(3 HIDROKSIBUTIRAT-KO-3-HIDROKSIVALERAT) DALAM LUMPUR Dewi, Asiska Permata; Octaviani, Melzi; Rustini, Rustini; Zaini, Erizal; Djamaan, Akmal
Jurnal Sains dan Teknologi Farmasi Vol 17, No 1 (2012)
Publisher : Universitas Andalas

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Abstract

An assay of biodegradation of plastic film combination of polystyrene (PS) and poli (3-hydroxy butyrate-ko-3-hydroxyvalerate) [P(3HB-ko-3HV)] has been conducted. The assay was done by using soil burial test method. The plastic film was made by blending technique of various composition of PS/P(3HB-ko-3HV) in to the mud. The remaining weight of plastic film was determined gravimentically time intervals 1,2,3,4 and 5 weeks of burial. Result showed that the P(3HB-ko-3HV) had an influence on the degradation rate of plastic film. The biodegradation rate increased with an increase in P(3HB-ko-3HV) content in the mixture. The rate was derived from linear regression equation and parameters such as biodegradation rate constant (k), t 50 % and t 95% were obtained. It was found that for combination of  PS/P(3HB-ko-3HV) 95:5 k= 1,26% b/b per week , t 50 % = 39,07 weeks and t 95 % = 74,783 week for the mixture of PS/P(3HB-ko-3HV) 90:10  k = 1,604 % b/b per week, t 50 % = 30,57 week and t 95 % = 58,62 week. For the mixture of PS/P(3HB-ko-3HV), 85:15 k = 2,663 % b/b per week, t 50 % = 17,95 week and t 95 % = 34,85 week. While those of pure PS plastic film k = 0,24 % b/b per week, t 50 % = 201,85 week and t 95 % = 354,55 week which indicated a very slow biodegradation. Analysis by SEM showed that the surface of plastic film was eroded and damaged during the burial. The amount of bacterial colony per 1 gram of mud was 2,31x107.
Biosynthesis of a biopolymer poly(3-hydroxybutyrate) from a mixture of palm oil and 2-butanol as carbon sources Djamaan, Akmal
INDONESIAN JOURNAL OF PHARMACY Vol 22 No 4, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.566 KB) | DOI: 10.14499/indonesianjpharm0iss0pp315-322

Abstract

Polyhydroxybutyrate  [P(3HB)]  are  synthesized  as  carbon  and  energy reserve materials by many types of microorganisms under certain environmental condition. These biosynthetic polyesters have much received attention as they can  be  considered  to  be  a  source  for  developing  novel  biodegradable  plastic materials for pharmaceutical, medical and biodegradable packages industries. In this case,  Erwiniasp. USMI-20, a locally soil isolated microorganism has been found  to accumulate  P(3HB)  in its  cells  during  growth  on  mineral-media  with  a mixture of palm oil and 2-butanol as carbon source. Fermentation process was conducted  through  a  feedbatch  cultivation  under  aerobic  condition  at  pH  7.0, incubation temperature 30 oC, and agitation rate of 200 rpm for 48 hours. The characterization of the  polymer production  was based  on  cell growth  (biomass) and  polymer  content  detected  by  a  gas  chromatography  methode.  Result showed  that  from  a  mixture  of palm  oil:2-butanol  (4.62g/L+0.89g/L) produced P(3HB) of 50.86 %w/w with biomass of 5.82g/L; a mixture of palm oil:2-butanol (4.62g/L+1.65g/L) produced  P(3HB)  of 57,77%  w/w  with  biomas  of 6.01g/L,  a mixture  of  palm  oil:2-butanol  (4.62g/L+2.48g/L)  produced  P(3HB)  of  55.85%w/w with  biomass  of  6.57g/L,   a  mixture  of palm  oil:2-butanol (4.62g/L+3.29 g/L) produced P(3HB) of 56.37% w/w with biomass of 6.67g/L and a mixture of palm  oil:2-butanol  (4.62g/L+4.12g/L)  produced  P(3HB)  of  47.70%  w/w  with biomass of 6.33g/LKey words:biopolymer, poly(3-hydroxybutyrate), palm oil, and n-butanol.
PENGARUH PENGGUNAAN PENYALUT BIOBLEND PS/PCL TERHADAP PELEPASAN ZAT AKTIF UREA GRANUL -, Salman; -, Febriyenti; Djamaan, Akmal
Jurnal Riset Kimia Vol 8, No 2 (2015): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v8i2.234

Abstract

The most widely used surfactant is an anionic surfactant which is synthesized from petroleum namely Linear Alkylbenzene sulphonate (LABS). Methyl Ester Sulfonate which is currently being developed. Surfactant can produced from palm oil methyl ester via sulfonation sulfonate. When in this research using sodium metabisulphite. The aims of this work is to synthesize Methyl Ester Sulfonate surfactant from Palm Oil Methyl Ester using Sodium Metabisulphite and a catalyst Calcium Oxide. The effects of time and the mole ratio are also investigated. Sulfonation process carried out in 4, 5, 6 hours with mole ratio of 1: 0,5, 1:1, 1: 1,5, temperature of 80° C and with stirring speed of 450 rpm. It haven been found that the surfactant produced has density of (0.89490 g/cm3 - 0.89545 g/cm3), viscosity (2.0323 cP - 2.1329 cP), pH (2,03 - 2,48), surface tension (32.60 mN/m - 33.60 mN/m), interfacial tension (30.45 mN/m - 30.94 mN/m), and the stability emulsion (59.17% - 89, 17%).
PRODUCTION AND CHARACTERIZATION OF A BIODEGRADABLE POLYMER POLY(3-HYDROXYBUTYRIC ACID) FROM GLUCOSE USING Erwinia sp. USMI-20 Akmal Djamaan; Mohamed Isa Abd. Majid; Mohd. Azizan Mohd. Noor
Indonesian Journal of Pharmacy Vol 12 No 4, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.474 KB) | DOI: 10.14499/indonesianjpharm0iss0pp172-180

Abstract

The production and characterization of a biodegradable polymer poly(3-hydroxybutyric acid), P(3HB) from glucose as sole carbon source by Erwinia sp. USMI-20 has been carried out. Results showed that Erwinia sp. USMI-20 could produced P(3HB) with a maximum polymer content of 48 % of the dry cell weight, an amount of polymer of 2.8 g/l, a dry cell weight of 5.8 g/l, a maximum specific growth rate of 0.21 h-1. a maximum polymer production rate of 0.02/h, yield YP(3HB)//C of 0.21 g/g, with the optimum fermentation time of 48 hours. The melting temperature (T) and glass transition temperature (Tg) of the P(3HB) were 175 ˚C and 15 ˚C, respectively. The weigh-average molecular weight (Mw) was in the range of 1,000,000 to 1,120,000 D whereas the number-average molecular weight (Mn) was in the range of 420,000 to 580,000 D with the polydispersity index (Mw,Mn) in range of 1.9 to 2.4. Key word: biodegradable polymer, glucose and Erwina sp.
Optimasi Proses Produksi Bioplastik Poli (3-Hidroksibutirat) dengan Bakteri Bacillus Sp Faac 20801 Menggunakan Bahan Dasar Jerami Padi Secara Fermentasi Krisyanella, Krisyanella; Djamaan, Akmal; Aulia, Witra
Jurnal Sains dan Teknologi Farmasi Vol 17 No 1 (2012)
Publisher : Fakultas Farmasi Universitas Andalas

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Abstract

Optimization of a production of a bioplastic poly (3 - hidroxybutirat) (P(3HB) by fermentation using Bacillus sp FAAC 20801 bacteria and rice straw as carbon source has been carried out. Detection of P(3HB) was done using gas chromatography. The highest glucose level obtain by using H2SO4 5% for hydrolisis. 46,800 mg straw (glucose concentration 200 mg/ml) were obtained 5,379 mg biomass and 0.058 mg P(3HB) ; from 83,400 mg rice straw (glucose concentration 400 mg/ml) were obtained 5,011 mg biomass and 0.124 mg P(3HB) ; from 142,800 mg rice straw (glucose concentration 600 mg/ml) were obtained 3,992 mg biomass and 2.309 mg P(3HB). 
Kajian Biodegradasi Filem Plastik Campuran Polimer Sintetik dengan Biopolimer dalam Larutan Air Octaviani, Melzi; Dewi, Asiska Permata; Boestari, Agusri; Zaini, Erizal; Djamaan, Akmal
Jurnal Sains dan Teknologi Farmasi Vol 17 No 1 (2012)
Publisher : Fakultas Farmasi Universitas Andalas

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Abstract

A study on degradation of plastic film of the mixture of polystyrene sintetic polymers (PS) with poli(3-hidroxyibutirat) [P(3HB)] dan poli(3-hidroksibutirat-ko-3-hidroxyvalerat) [P(3HB-ko-3HV)] biopolymers in-vitro and in-vivo has been done. The assay was done by immersion test method in aquadest, river water at various pH of 4,6,8, and 10 respectively. Degradation rate was determined by linear regression with parameters including : t 50%, t 95% and rate constant (k). Degradation rate of plastic film of 1 PS/P(3HB) 95:5 at pH 6 was the fastest with k = 0,79 % week, t 50 % = 63,06 week and t 95 % = 119,84 week, followed by PS/P(3HB) 90 : 10 with k = 1,22 % / week, t 50 % = 41,25 week and t 95 % = 78,21 week and PS/P(3HB) 80 : 20 with k = 3,16 % week, t 50 % = 15,81 week and t 95 % = 30,05 week while pure PS plastic film (100 : 0 ) did not degraded at all. The degradation rate of a mixture of plastic film in river water, PS/P(3HB-ko-3HV) 95:5 showed k= 0,64 % week, t 50 % = 82,63 week and t 95 % = 157,17 week, PS/P(3HB-ko-3HV) 90:10 with k = 0,69 % week, t 50 % = 72,16 week and t 95 % = 137,4 week ; PS/P(3HB-ko-3HV) 85:15 with k = 0,74 %/week, t 50% = 66,79 week and t 95% = 127,32 week, while the pure PS plastic film (100:0) did not degrade.
Analisis Faktor-Faktor Yang Mempengaruhi Kepuasan Kerja Apoteker Yang Bekerja di Apotek di Kota Padang Djamaan, Akmal; Syahrul, Laura; Bakhtra, Dwi Dinni Aulia
Jurnal Sains dan Teknologi Farmasi Vol 18 No 1 (2013)
Publisher : Fakultas Farmasi Universitas Andalas

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Abstract

Humans resources must be evaluated as a key factor in an organizations success. Humans resource organization has a wide range of needs. Job satisfaction is a reflection of the employees attitude towards the overall feeling of the work, it consists of various aspects. Job satisfaction can improve service quality and increase employee satisfaction. This was a descriptive study about pharmacists who work at a pharmacy in Padang city as much as 50 respondent. There are six variables that influence job satisfaction of pharmacists: working conditions, salary, promotion, fair treatment, job security, relationships with co-workers and the relationship with the leader. Results showed that the pharmacists working at pharmacies in Padang was just quite satisfied with the work. There was a variable relationship between co-workers and the job satisfaction of the respondents in the amount of 76.32%. Total achievements of the respondents to the relationship with the fair treatment of 75.76 %, 75.68% working conditions, relations with the leaders of 73.92%, 72.79% salary and promotion. Variables of job security less the total respondents 59.80% achievement . Differences in demographic factors also affect the job satisfaction of the respondents. Job satisfaction of male respondents are influenced by working conditions, while the female respondents are affected by fair treatment and relationships with co-workers. Respondents who have a service life over 5 years shows that work satisfaction is strongly influenced by the variable of fair treatment while his tenure of 1 to 5 years are affected by working conditions. Respondents who have over 30 years of age are affected by work satisfaction and working conditions for the age 24 to 30 years of work satisfaction is strongly influenced by fair treatment.