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Al-Kimia

al-kimia
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Published by Universitas Islam Negeri Alauddin Makassar

ISSN : 23022736 EISSN : - DOI : -

Core Subject : Science,

Chemistry

Al-Kimia is a journal of chemistry that published by Department of Chemistry, Faculty of Science and Technology UIN Alauddin Makassar. The journal is published twice a year on January-June and July-December. This journal encompasses original research articles in all aspects of chemistry and related area. The journal, which was firstly published in July 2013, is a forum for communication of research results, review the results of research, methodologies and new approaches in research related to chemistry in Indonesia.

Arjuna Subject : -

Articles 8 Documents

Search results for , issue "Vol 2 No 2 (2014): Desember" : 8 Documents clear

Isolasi Dan Identifikasi Bakteri Termofil Penghasil Amilase Dari Sumber Air Panas Lejja Sulawesi Selatan Rugaiyah A. Arfah; Abd. Rauf Patong; Ahyar Ahmad; M. Natsir Djide
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

A Research isolation and identification of bacteria termofil amylase from hot springs Lejja South Sulawesi has been done. This study aims to characterize the morphological, biochemical, genus and species of bacteria producing the enzyme amylase. The method used in this study through the stages: 1) Skrening and isolation of bacteria by means of as much as 1.0 mL of sample dilution plated on Petri dishes containing agar medium, then incubated for 20-24 hours at 50 °C, colonies of bacteria growing and has a colony morphology different character each taken 1 ose then etched into the amylolytic selective medium then incubated for 20-24 hours at 40oC and 50oC. Colonies that grew on selective media is scratched quadrant amylolytic to obtain pure isolates. Pure bacterial isolates taken 1 ose then grown in selective medium for 48 h at 50° C, bacterial isolates were grown spilled iodine solution (2% I2 and 0.2% KI) when there is a clearing zone around the colony indicated as the enzyme-producing bacterial isolates termofil amylase; 2) termofil characterization of bacterial isolates in microscopy with Gram stain; 3) isolates selected biochemical tests performed according to the method Bergey's Manual and Systematic of Bacteriology. Results of screening and isolation of 10 bacterial isolates obtained amylase through iodine test, selected 2 isolates, 1 isolate from water samples RSAII-1B and 1 isolates from water samples mixed sediment RSSII-4B, which has a diameter of clearing zone of 5.6 cm respectively and 5.15 cm; out such characterization results of gram stain microscopy showed that the 2 isolates including gram + and shaped bacillus, the colony morphology as observed macroscopically, microscopy and biochemical test results obtained RSAII isolates and isolates RSSII-1B-4B is a Bacillus sp.

Isolasi dan Implementasi Protein Bioaktif Kepah (Atactodea striata) Sebagai Bahan Obat Antibakteri Tahirah Hasan; Abd. Rauf Patong; Abd. Wahid Wahab; M. Natsir Djide
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

This study aimed to 1) determine the degree of saturation of ammonium sulfate right to extract and purify the bioactive protein from shells (Atactodea striata), 2) determine the fraction of active protein from shells (Atactodea striata) as a potential antibacterial. In this study used the Lowry method for determine protein concentration and agar diffusion method for antibacterial activity. Extraction of shells Atactodea striata was conducted by making use of buffer solution (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl2, 1 % β-mercaptoethanol, and 0.5 % Triton X-100). Purification of proteins by precipitation using ammonium sulfate at saturation level 30 %, 50 %, 70 %, and 90 %. The results showed that the protein concentration of the crude extract is 41.6354 mg/mL. At fractionation rate of 0-90% saturation showed the highest concentration of protein found in fractions with 70% saturation level is 56.4184 mg/mL. The testing of antibacterial activity against Staphylococcus aureus showed that crude extracts and protein fractions Atactodea striata is considered effective as an antibacterial. The highest bioactivity during 24-hour incubation in protein fractions obtained by ammonium sulfate saturation level of 50% is 25.17 mm. Whereas the lowest activity was obtained at 90% saturation level is 14.05 mm. Bioactivity against Escherichia coli after incubation for 24 hours has the highest activity in the protein fraction with 30% ammonium sulfate saturation is 15.12 mm. Whereas the lowest activity was showed at 70% saturation level is 10.30 mm. After the observation was continued for 48 hours on both test bacteria, which formed a clear area becomes cloudy. It shows that the crude extract and fractions of protein tend to be bacteriostatic against Staphylococcus aureus and Escherichia coli.

Penggunaan Epiklorohidrin Dalam Meningkatkan Stabilitas Adsorben Kitosan Berikatan Silang Dan Kitosan Tercetak Ionik Kobal Muhammad Qaddafi; Abd. Wahid Wahab; Nursiah La Nafie; Paulina Taba
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

The use of epichlorohydrin to improve the stability of chitosan adsorbent has been carried out. The purpose of this study was to determine the stability of the chitosan adsorbent after modified into epichlorohydrin cross-linked chitosan and ionic cobalt imprinted adsorbent chitosan cross-linked epichlorohydrin. Adsorbent prepared by homogeneous gel formation of chitosan in acetic acid medium and then modified into epichlorohydrin cross-linked chitosan beads and ionic cobalt imprinted adsorbent chitosan cross-linked epichlorohydrin. Stability of the adsorbent was determined by calculating the percentage of insoluble material after stirring the adsorbent in nitric acid medium at pH 1, 2, and 3 for 12 hours. The results of FTIR spectra show a shift of the absorption band of chitosan were compared with epichlorohydrin cross-linked chitosan and ionic cobalt imprinted adsorbent chitosan cross-linked epichlorohydrin. The use of epichlorohydrin as cross-linked agent may increase the stability of the adsorbent to maintain the percentage of insoluble material by 89% at pH 3 for epichlorohydrin cross-linked chitosan adsorbent and 96% at pH 3 for ionic cobalt imprinted adsorbent chitosan cross- linked epichlorohydrin.

Isolasi Dan Karakterisasi Bakteri Simbion Larva Kupu-Kupu Cossus cossus Penghasil Enzim Selulase Maswati Baharuddin; Abd. Rauf Patong; Ahyar Ahmad; Nursiah La Nafie
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

This study was conducted to characterize bacterial symbionts of butterfly larvae Cossus cossus capable of degrading cellulose. This study successfully purify and characterize isolates originating from the intestine (CC1 and CC2), head (CC3), middle (CC4), and tail (CC5). From a qualitative test using 0.1% congo red gained the clear zone indicates that the bacteria are able to degrade cellulose. Based on the test temperature and pH on the growth of the data obtained CC5 isolates including isolates of thermophilic bacteria, while others including mesophilic bacteria. While based pH test all isolates were able to grow well at neutral pH. Based on the data obtained growth curve maximum bacterial growth at the 24th hour. Based on morphology and physiology test obtained bacteria genus Acinotobacter, Pseudomonas, and Bacillus.

Pemanfaatan Kitin Sebagai Bahan Membran Elektroda Enzim Diamin Oksidase Untuk Biosensor Histamin Abdul Karim; Abd. Rauf Patong; Abd. Wahid Wahab; Indah Raya
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

This research aims to utilize isolated chitin from shrimp waste to develop histamine biosensors basedon diamine oxidase (DAO) enzyme electrode with cyclic voltammetry. DAO enzyme trapped in the chitin-cellulose acetate membranes with various comparisons were layered on the Pt electrode. Histamine will be oxidized by the DAO enzyme produces aldehydes and H2O2 which acts as an electron transfer mediator. Biosensor performance is influenced by several factors, especially the concentration and composition of electrode membranei.Comparison of chitin-cellulose acetate used in this study were 1:1, 2:1 and 3:1. Isolated chitin from the shrimp waste is chemically obtainedrendamen of 23.6%, and characterization of electrode membrane by FTIR and cyclic voltammetry showed that the DAO enzymes electrode with chitin-cellulose acetate membrane 2:1 is the best composition.

Biosorpsi Tanaman Puring (Codiaeum variegatum) Terhadap Emisi Timbal (Pb) Pada Kendaraan Bermotor Nur Amalia P; Syamsidar HS; Kurnia Ramadani
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Enhancement the motorized vehicle number will be potential for air pollution in the big cities. One of the pollutant which is resulted from burning process fuel is timbale (Pb) metal. Alternative effort that is used to decrease the higher timbale (Pb) metal pollutantmaterial is with manner biosorption use puring plant (codiaeumvariegatum).The aim of this research is to know the puring plantability in absorbs Pb metal. The treatment methodis used to sprinkling plant and without sprinkling and than directly exposure to plant with period 3,6 and 9 days. The result of research that puring leaves ability can absorb Pb concentration metal between (1 until 1,5) ppm or 0,304 mg/Kg at nine days of period exposure, it order can be considered as biosorption agent Pb metal pollution.

Identifikasi Metabolit Sekunder Ekstrak Etil Asetat Biji Alpukat (Persea americana Mill.) dan Uji Toksisitas Terhadap Larva Udang Artemia salina Leach. Nurdia Asdar; Asriani Ilyas; Maswati Baharuddin
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Identification of metabolite compound in acetone Extract from Fruits Avocado seed (Percea americana Mill) and toxicity test against Artemia salina Leach. Aim of this research is to identify the secondary metabolite compound in aceton extract from fruits Avocado seed (Percea americana Mill) and to determine the potential of secondary metabolites from an avocado extract as anticancer. The compound was obtained from Identification process in several stages, namely extraction, fractination, purification and identification. The identification process was color test, TLC, spectroscopy IR. The toxicity test by Brine Shrimp Lethality Test (BSLT) to Artemia salina Leach. The compound obtained from this research was yellow-white crystalline needle-shaped, the purity test with TLC analysis showed a stain in three eluen system on the stain test on three eluent systems with Rf proportions as follows; 0,15 from chloroform:ethyl acetat (6:4), 0,33 from methanol:chloroform (1:9), 0,75 for acetone:ethyl acetat, and positively to the reagent Wagner and gave brown precipitate for indicate as alcaloid group. This result is supported by spectroscopy from compound.While toxicity tests showed condensed acetone extracts and pure compounds are toxic with LC50 value of each is 20.61 mg/mL and 39,81 mg/mL.

Analisis Logam Merkuri (Hg) Pada Krim Pemutih Wajah Merek X Dengan Metode Spektrofotometri Serapan Atom (SSA) Madania Madania; Megawati M Martani
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Whitening creams that supposedly contain mercury which is added with the aim to reduce freckles on the skin’s surface, but it gives a negative impact, for it was conducted analysis of the presence of mercury compounds qualitatively and quantitatively. This research is purely experimental research with the aim of identifying the content of mercury (Hg) is the bleach cream face on brand X circulating in Indonesia are on the label does not have the composition, number and batch number POM and determine levels of mercury (Hg) is the bleach cream face on brand X. Qualitative test using three reagents that NaOH, HCl, KI whereas quantitative testing using atomic absorption spectrophotometry (SSA).The result of the research that brand X contains mercury (Hg). Brand X code A contains 2718,1273 bpm mercury (Hg) and code B contains 214,1661 bpm. Quantitative analysis results calculates using concentration obtained from a linear regression of whitening face cream brand X Code A contains 3239.9117 bpm mercury (Hg) and Code B contains 858,6798 bpm mercury (Hg) in pot.

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