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Jurnal Kimia Terapan Indonesia

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08532788 EISSN : 25277669 DOI : -

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Articles 7 Documents

Search results for , issue "Vol 21, No 1 (2019)" : 7 Documents clear

Characterization of Protease Crude Extract from Indigenous Lactic Acid Bacteria and the Protein Degradation Capacity in Local Tuber and Cereal Paste Flour Tatik Khusniati; Nanda Sabbaha Nur Kasfillah; Vilya Syafriana; Resti Sofia Zahara; Padmono Citroreksoko; Sulistiani Sulistiani; Trisanti Anindyawati
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Protease hidrolyzed protein in flour in order to more digest by human ulcer. Lactobacillus plantarum B110 and Lactobacillus satsumensis are indigenous lactic acid bacteria that produce protease. The objective of this research is to characterization of protease crude extract from indigenous lactic acid bacteria and the protein degradation capacity in local tuber and cereal paste flour. Tuber and cereal flour used were purple sweet potato (Dioscorea alata), cassava (Manihot esculenta), rice (Oryza sativa), corn (Zea mays) and wheat (Triticum) as comparison. Proteaseactivity was tested by Horikoshi method (1971) and protein degradation was by formol titration. Research results showed that optimum activities and stabilities of Lactobacillus plantarum B110 were at pH: 7.5, 45oC and pH:5.0-8.0, 35-50oC, while that L. satsumensis EN 38-32 were at pH: 7.0, 40oC and pH:6.0-8.0, 20-45oC. Increases in protein degradation capacity of the paste flour additional proteases crude extract from L. plantarum B110 were 0.0838% (purple sweat potato), 1.3299% (cassava), 0.5834% (corn), 0.7499% (rice) and 1.5551% (wheat as comparison); while that L. satsumensis EN 38-32 were 0.20% (purple sweet potato), 0.32% (cassava), 0.87% (corn), 1.17% (rice). Based on increases in protein degradation capacity, protease crude extract from L. plantarum B110Â and L. satsumensis EN 38- 32 were sequently better to hidrolyze protein of cassava and rice paste flour than thatother tuber and cereal.

The Effect of Acid Hydrolysis Treatment on the Production of Nanocellulose Based on Oil Palm Empty Fruit Bunches Athanasia Amanda Septevani; Dian Burhani; Yulianti Sampora; Yenni Apriliany Devy; Gita Novi Ariani; Sudirman Sudirman; Dewi Sondari; Khairatun Najwa Mohd Amin
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Nanocellulose has been known as promising reinforcing material in various polymer based product resulted to remarkable improvement in mechanical and thermal properties. Hence, studies to date have developed and explored various sources of biomass to produce nanocellulose. The aims of this study are to synthesize and fully characterize nanocellulose obtained from abundantly available oil palm empty fruit bunches via two different methods which are strong (H2SO4) and mild acid (H3PO4) hydrolysis at 50 Â°C for 3.5 hours. Based on the morphological study using Transmission Electron Microscopy, rod like nanocellulose was obtained using strong acid hydrolysis while mild acid hydrolysis produced long filament shape. X-Ray diffraction analysis showed that the degree crystallinity of nanocellulose produced from strong acid hydrolysis was higher which is 96% than that of mild acid hydrolysis recorded with 86%. While the sulphuric acid hydrolysis usually produces lower thermal stability than that of other types acid hydrolysis, surprisingly, in this study, the thermal stability of nanocellulose from strong acid hydrolysis was relatively similar to mild acid hydrolysis due to the formation of single crystal structure affording unique characteristic of the obtained nanocellulose.

Preliminary Economic Study on the Production of ZnO Nanoparticles Using a Sol-Gel Synthesis Method Fikri Aziz Shalahuddin; Sera Serinda Almekahdinah; Asep Bayu Dani Nandiyanto
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

The economic evaluation is one of the key points in building chemical industries. This paper presented a preliminary economic evaluation of the large scale production of zinc oxide (ZnO) nanoparticles using the sol-gel method, which is very useful for helping decision whether the fabrication of this material profitable or not. Particularly, the study was done by changing the cost of raw material, which was compared to several economic parameters such as GPM, PBP, and CNPV. The result showed that the project was profitable by increasing raw material cost below 100% from the estimated raw material cost, informing the fact for the prospective fabrication for fulfilling the demand of ZnO nanoparticles.

Self-Healing Concrete Using Bacteria Calcification from Karst Cave Environment Ananto Nugroho; Agung Sumarno; Luna Nurdianti Ngeljaratan; Deni Zulfiana; Ni Putu Ratna Ayu Krishanti; Triastutil Triastutil; Eko Widodo
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Karst regions in Indonesia have the uniqueness of the landscape and biodiversity. The karst is formed by the dissolution of rocks and the precipitation of mineral. In the cave, there are ornaments of stalactite and stalagmite which are formed by the process of mineral precipitation. We have isolated, screened, and identified the soil bacterium from the cave environment (Lysinibacillus macroides). These bacteria are able to precipitate calcium carbonate and can be developed as a self-healing agent concrete. We investigated the proportions and the properties of mixtures concrete containing lightweight aggregate and volcanic ash impregnated with bacteria. A comparison study was made by concrete cylinders subjected to compressive strength tests with and without the bacteria. It found that the strength of concrete with bacteria decreased by less than 10.56% for 28 days of cured specimens. This study showed that the effects of bacteria on the strength of concrete are not considerable. However, these bacteria are effective to repair in the microcrack less than 0.3 mm

Use of Mg-Al/hydrotalcite Catalyst in Biodiesel Production from Avocado Seed Oils: A Preliminary Study Irvan Maulana Firdaus; Tri Fitriany; Milda Nurul Hidayah; Agus Soleh; Khilman Husna Pratama; Febiyanto Febiyanto
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Biodiesel production of avocado seed oils has been carried out using the heterogeneous catalyst of Mg-Al/hydrotalcite. Transesterification process was conducted by varying temperature reaction andÂ oil-methanol molar ratio. The reaction temperature was 30, 40, 50, and 60Â°C, whereas the oil-methanol molar ratio was 1:3, 1:6, 1:9, and 1:12, respectively. As-synthesized Mg-Al/hydrotalcite catalyst was characterized using X-ray diffraction and FTIR. Meanwhile, the biodiesel was analyzed their density, viscosity, water content and 1H-NMR analysis. The results showed that optimum condition in biodiesel production wasÂ oil-methanol molar ratio of 1:6 at a reaction temperature of 60Â°C for 60 minutes and catalyst quantity of 2% yielding biodiesel conversion percentage was approximately 15.90%. However, this preliminary findings showed that Mg-Al/hydrotalcite was able to convert the avocado seed oils into biodiesel even if still need further analysis and research so that produces a higher percentage of biodiesel conversion.

Conversion of hemicellulose from kenaf core fiber to xylose through dilute sulfuric acid hydrolysis William Judiawan; Yanni Sudiyani; Elda Nurnasari
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Kenaf (Hibiscus cannabinus) is a lignocellulosic plant that is usually utilized as a fiber source for sack production. The core from kenaf fiber has not been utilized yet in Indonesia, therefore it is still considered as a waste. Hemicellulose from kenaf core can be hydrolyzed to xylose through dilute sulfuric acid hydrolysis in high temperature. Hydrolysis in this study was done by using autoclave at 121â„ƒ and 10% (m/v) biomass: acid ratio for 15 and 45 minutes with a variation on acid concentration (2%, 4%, and 6% v/v). Xylose concentration in the hydrolyzate tends to increase with higher acid concentration and longer heating time. 6% (v/v) sulfuric acid concentration and 45 minutes of heating time produce the highest xylose concentration (20.53 gr/L) and yield (86.50%)

Phenol Biodegradation and Catechol 2,3-Dioxygenase Gene Sequencing of Bacillus cereus IrC2 isolated from Rungkut Indonesia Candra Yulius Tahya; Wahyu Irawati; Friska Juliana Purba
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Phenol is toxic organic compounds that harmful to humans, mammals, and disrupt the aquatic environment, especially higher-organisms in fresh-water environment. The biodegradation method using bacteria to degrade hazardous chemical and detoxify wastewater is an effective and efficient method. Bacillus cereus IrC2 isolated from sludge in an industrial wastewater treatment plant in Rungkut â€“ East Java, Indonesia has been examined for the ability to degrade phenols in minimal salt medium. Bacillus cereus IrC2 is Gram-positive bacterium. This bacterium is motile, rod-shaped and its nucleotides sequence of 16S rRNA gene has been sequenced and can be accessed in GenBank with accession number MK511840. Bacillus cereus IrC2 is capable to use phenol up to 400 ppm as the sole carbon source to grow for 48 hours incubation. Phenol degrades 96% from initial concentration. Degradation of phenol was calculated by colorimetric method using 4-aminoantipyrine reagent and confirmed by GC MS analysis. The aerobic degradation of phenol pathways consists of three steps; in the first step, two hydroxyl groups are inserted into aromatic ring and catalyzed by mono or dioxygenase to produce dihydroxy aromatic compounds which are mostly catechols. Catechol enters the next step of aromatic ring cleavage catalyzed by catechol 1,2-dioxygenase and/or catechol 2,3- dioxygenase. The catechol 2,3-dioxygenase gene of Bacillus cereus IrC2 has been amplified by PCR and cloned into pTA2 vector. The cloned plasmid (pTA2-catE) was transformed into E. coli DH5Î± and selected blue-white colonies. The insert sequence was determined by Sanger deoxy sequencing method. The catechol 2,3-dioxygenase gene nucleotides sequence of Bacillus cereus IrC2 was submitted nto GenBank with accession number MK561609.

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