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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 7 Documents
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Search results for , issue "Vol. 18 No. 4 (2011): December 2011" : 7 Documents clear
Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from . NURBARIAH; ITA DJUWITA; KUSDIANTORO MOHAMAD; IMAN SUPRIATNA
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.151

Abstract

Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.
Coral reef communities contain a wide variety of mutualistic associations none more important than the relationship between corals and their symbiotic dinoflagellates of the genus Symbiodinium sp., commonly referred to as zooxanthellae. The function of Zinc (Zn) as cofactor of several enzyme systems such as extracellular carbonic anhydrase (extracellular CA) which catalyzes the interconversion of HCO3- and CO2. Concentrations of dissolved Zn in oligothropic waters are often very low therefore ma WIDIASTUTI KARIM; RICHARDUS KASWADJI; TRI PRARTONO; LILY MARIA GORETTI PANGGABEAN
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.157

Abstract

Coral reef communities contain a wide variety of mutualistic associations none more important than the relationship between corals and their symbiotic dinoflagellates of the genus Symbiodinium sp., commonly referred to as zooxanthellae. The function of Zinc (Zn) as cofactor of several enzyme systems such as extracellular carbonic anhydrase (extracellular CA) which catalyzes the interconversion of HCO3- and CO2. Concentrations of dissolved Zn in oligothropic waters are often very low therefore may limit the growth of zooxanthellae and their ability to fix CO2 from seawater via the carbonic anhydrase. The aim of this research is to investigate the effect of various concentrations of Zn on the growth and extracellular CA activity in zooxanthellae. Cell density was monitored daily by enumeration with hemocytometer-type chamber (0.1 mm). Extracellular CA was measured in homogenized intact whole cell by a pH drift assay. Results revealed that Zn status strongly influences the growth rate and extracelullar CA activity in zooxanthellae. The specific growth rate and cell density increased two-fold whilst extracelullar CA activity increased 10.5 times higher than that in control with increasing concentrations of Zn from 0 to 80 nM, but decreased when Zn was over 80 nM. Under a concentration of 80 nM was not Zn limited culture, consequently the growth rate of zooxanthellae not dependent on CO2 concentration yet offset by extracelullar CA activity.
The javan green peafowl (Pavo muticus muticus) is a endangered bird species. They have big pressure to the population and the habitat. The fact that the birds are still exist on their local distribution. Behavior ecology of javan green peafowl is dealing with activities, mechanism and strategies of the birds in relation to their environment. The aimed of the study is to obtain data and information of ecological adaptation strategies through the behavior activities, mechanism and strategies of ja JARWADI BUDI HERNOWO; ANI MARDIASTUTI; HADI SUKADI ALIKODRA; CECEP KUSMANA
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.164

Abstract

The javan green peafowl (Pavo muticus muticus) is a endangered bird species. They have big pressure to the population and the habitat. The fact that the birds are still exist on their local distribution. Behavior ecology of javan green peafowl is dealing with activities, mechanism and strategies of the birds in relation to their environment. The aimed of the study is to obtain data and information of ecological adaptation strategies through the behavior activities, mechanism and strategies of javan green peafowl in-relation to their habitat types. Ad libitum sampling method was used on continuous recording every peafowl activities in relation with duration, frequencies mechanism and strategy were influenced by habitat type’s condition. Chi-squre test was used for statistical analysis to know different behavior has influenced by habitat types. The result shown that the javan green peafowl habitat typse has significant influenced on duration of the activities but not at the activities pattern. The walking during the feeding, select shading places or luxuriant trees close to feeding site, choosing tall trees or emergent trees close to open area as roost site, open area also road as display or dancing area, open area which grow shrubs as nesting site are javan green peafowl ecological behavior strategies. The mechanism and strategies of javan green peafowl behavior ecology are related to avoid from disturbance and to adapt their habitat type’s conditions.
Human brain posseses the ability to create a concept to assist the process of grouping individual object or events into different classes or categories. We call this grouping process as categorization. In addition to humans, the ability to categorize has also been proposed for animals. Being able to identify, visually or otherwise, a new object as a member of a category is an advantage for animals. Present experiment aims to test the categorization ability in discriminating species by Macaca fas KANTHI ARUM WIDAYATI; BAMBANG SURYOBROTO; ACHMAD FARAJALLAH; AKICHIKA MIKAMI
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.177

Abstract

Human brain posseses the ability to create a concept to assist the process of grouping individual object or events into different classes or categories. We call this grouping process as categorization. In addition to humans, the ability to categorize has also been proposed for animals. Being able to identify, visually or otherwise, a new object as a member of a category is an advantage for animals. Present experiment aims to test the categorization ability in discriminating species by Macaca fascicularis. Using match-to-sample task with photographs of monkeys and human as stimuli, we tested whether monkeys able to categorize monkey individuals as a class against human individuals as another class. We found that monkeys categorized humans differently from monkeys. The monkeys used physical characteristic such as shape and colors from the photographs to create different concepts of human and monkeys.
Growth of body size (body height and body weight) and somatotype in 363 girls and 299 boys aged 4 to 20 years of ethnic Javanese lived in Magelang Regency Indonesia were studied cross-sectionally. Over half of them were categorized in the well-off family, therefore underweight or underfat prevalence in our subjects was low (14.3%) but overweight and obesity prevalence was also low (14%). They were shorter and lighter than reference children from U.S., Japan and Yogyakarta but they improved when TETRI WIDIYANI; BAMBANG SURYOBROTO; SRI BUDIARTI; ALEX HARTANA
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.182

Abstract

Growth of body size (body height and body weight) and somatotype in 363 girls and 299 boys aged 4 to 20 years of ethnic Javanese lived in Magelang Regency Indonesia were studied cross-sectionally. Over half of them were categorized in the well-off family, therefore underweight or underfat prevalence in our subjects was low (14.3%) but overweight and obesity prevalence was also low (14%). They were shorter and lighter than reference children from U.S., Japan and Yogyakarta but they improved when compared with those of the same ethnic of Bantul and with the different ethnic of rural India. There was a clear age-related change of their somatotype. At age 4 years, the physique of children subjects in both sexes is found to be mesomorph-endomorph. Thereafter it is transformed into ectomorphic-endomorph in girls and to mesomorph-ectomorph in boys at the age of 20 years. In girl subjects, the onset of puberty was characterized by an acceleration of endomorphy component at age 8 years. While in our boys it was characterized by an acceleration of ectomorphy since age 9 years. The different growth pattern of somatotype components showed that the use of BMI as an indicator of fatness in children should be reassessed.
Bioflocculant producing-bacteria from tapioca waste water were characterized. Two bacterial isolates i.e. LT-5 and LT-6 isolates had high flocculation activity, with activity of 68.92 and 71.38% respectively. The flocculation activity of LT-5 isolate increased at pH 2.0-4.0 (acidic condition), however the activity of LT-6 increased at pH 6.0-8.0 (neutral). Addition of 0.05% of AlCl3 as cation was the most effective and had important role in flocculation activity. Based on the morphological prope . SURYANI; LAKSMI AMBARSARI; I MADE ARTIKA; HARTUTIK EKA SUSANTI
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.193

Abstract

Bioflocculant producing-bacteria from tapioca waste water were characterized. Two bacterial isolates i.e. LT-5 and LT-6 isolates had high flocculation activity, with activity of 68.92 and 71.38% respectively. The flocculation activity of LT-5 isolate increased at pH 2.0-4.0 (acidic condition), however the activity of LT-6 increased at pH 6.0-8.0 (neutral). Addition of 0.05% of AlCl3 as cation was the most effective and had important role in flocculation activity. Based on the morphological properties, LT-5 isolate was identified as Chromobacterium violaceum and LT-6 isolate was identified as Citrobacter koseri.
Bartek is one of local melon varieties mainly cultivated in Pemalang, Central Java. Bartek has three variations of fruits; Long-Green, Ellips-Green, and Yellow. Chromosome characterization of the Bartek was investigated to determine the genetic variation. The main purpose of this research was to determine the genetic characters of Bartek including chromosome number, mitosis, cell cycle, and karyotype. Squash method was used for chromosome preparation. The results showed that all of Bartek observ BUDI SETIADI DARYONO; DIAN ARUNI KUMALAWATI
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.197

Abstract

Bartek is one of local melon varieties mainly cultivated in Pemalang, Central Java. Bartek has three variations of fruits; Long-Green, Ellips-Green, and Yellow. Chromosome characterization of the Bartek was investigated to determine the genetic variation. The main purpose of this research was to determine the genetic characters of Bartek including chromosome number, mitosis, cell cycle, and karyotype. Squash method was used for chromosome preparation. The results showed that all of Bartek observed in this study have similar diploid (2n) chromosome number = 24. According to the total number of chromosome, Bartek is closer to melon than cucumber. The mitotic analysis exhibited that the Bartek has similar karyotype formula, 2n = 2x = 24m. Based on the R value of the three kinds of Bartek (R < 0.27), it indicated that three kinds of Bartek were considered to be originated from similar species and one of melon varieties (Cucumis melo L. var. Bartek).

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