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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 7 Documents
 1 
Search results for , issue "Vol 7 No 1 (2001): December 2001" : 7 Documents clear
PENGGUNAAN AMILOGLUKOSIDASE DALAM PRODUKSI GULA CAIR DENGAN BAHAN DASAR UBIUBIAN Tatik Khusniyati; Abdul Choliq; E Djajakusuma; D Sastraatmadja
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/502

Abstract

Sugar cane limitation was found in refined sugar production, so it needed other alternative in sugar production with various cassava. Taro, sweet potato and cassava as high carbohydtrate of various cassava can be used in liquid sugar production by using microbial enzyme. Amiloglucosidase Mucorjavanicus can degrade various cassava carbohydrate. To know liquid sugar quality of various cassava, the use of amyloglucosidase Mucorjavanius in liquid sugar production with various cassava were researched. Observation were conducted with six treatments of amyloglucosidase concentration, that is 0.00% (control); 0.05%; 0.10%; 0.15%; 0.20% and 0.25%. the liquid sugar quality was detected by measuring reduction sugar (“DNS� method), water content (AOAC), pH, colour, and amyloglucosidase activity (“Samogeny� titration method). Statistical analysis used Complete Randomized Design. The result showed that the highest reduction sugar and amyloglucosidase activity on each sugar were found on liquid sugar 0.20% enzyme (p<0.05). the highest reduction sugar and amyloglucosidase activity, was respectively on Taro 323 mg/100ml and 54.91 unit/100 ml; sweet potato 242 mg/100 ml and 47.72 unit/100 ml; and cassava 188 mg/100 ml and 41.09 unit/100 ml. the lowest total solid or the highest water content between three sugars was on taro liquid sugar 0.20% enzyme, that is 64.97% and 35.03% respectively (p<0.05). pH liquid sugar onthree various cassava was on range between 5.78-6.01. the best colour was on taro liquid sugar, that is brown to yellow. From three sugars with different raw material, the best sugar quality was tari liquid sugar with 0.20%.
TRANSESTERIFIKASI ENZIMATIK ASAM LEMAK DARI SUBSTRAT MINYAK SAWIT DAN SANTAN KELAPA Joko Sulistyo; Yati Soedaryati Soeka; Rini Handayani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/504

Abstract

We have reported he potency of microbial extracellular enzyme for coconut oil by fermentation. Further investigation was aimed to study capacity if the enzyme on bioproses of crude palm oil by transesterification saturated fatty acid to fatty acid ester which is useful for industry of cosmetics, biopharmacy and natural chemistry. We have studied some lipase from culture filtrate of Bacillus subtilis FM-9101, Pseudomonas aerogenes FM-9201, Pseudomonas fluorescens FM-9202, Pseudomonas stutzerri FM 9203, and Candida rugosa FM-9301. Those five strains showed different activities during the hydrolysis substrate which resulted in decreasing or increasing free fatty acids those were liberated from media containing crude palm oil, coconut milk or mixed of both. The optimal transesterification condition were at temperature 45-50o C and pH 4.5 for C. rugosa and pH 6.0 tp 7.0 for P. aerogenes, P. fluorescens, P. stutzeri and B. subtilis. Under the enzyme concentration of 25% (v/v), the transesterification was effectively occurred, while at the concentration of 20% (v/v) the enzymatically biosynthesis required longer incubation period.
TIPE SITOLOGI DUA SPESIES PTERIS DALAM HUBUNGANNYA DENGAN KETINGGIAN TEMPA Siti Zubaidah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/505

Abstract

A cytological study of the two species of Pteris (Pteris biaurita and Pteris tripartita) growing around Malang was carried out. A hypothesis stated that ploidy was enchanched by cold weather. Those study want to find out the correlation between the altitude (with different temperatures) and ploidy of the two species. Cytological type and ploidy examined by chromosome number counting, using standart squash method. The result of this research showed that Pteris biaurita and Pteris tripartite have two cytotypes, 2n= 58 (2x or diploid) and 2n= 116 (4x or tetraploid). There was no correlation between the ploidy level of Pteris biaurita and the altitude, but the ploidy level of Pteris tripartite apt to be raise in higher altitude.
POLA INFEKSI ANEKA JENIS CAPLAK ACARINA:IXODIDAE PADA BIAWAK Varanus salvator DI BEBERAPA PELOSOK DAERAH INDONESIA Achmad Saim; Sri Hartini
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/507

Abstract

A total of 607 specimen (254 males, 140 femles, 92 nymph and 121 larvals) are found on 39 common water monitors from several areas in Indonesia, which are consisted of three generas (six species) of ticks. Total with discovery percentage each species of ticks on these common water-monitor are recorded 45 specimens (14%) Amblyomma helvolum , four specimens (2.6%) Aponomma komodoense, 573 specimens (71%) A. lucasi, 11 specimen (6.4%) A. sumbawaensis,eight specimens (6,4%) A. trimaculatuum, and two specimen (2.6%) Darmacentor atrosignatus. Adult of ticks and A. lucasi are found much more than other developmental stages or also its species. Its infection show of 1-4 stages or 1-2 species of ticks on each common water monitor and its parasitic index are recorded 15.6 or 1-121 of ticks on each common water-monitor. Its host, distribution and roles in ecosystem as parasites and vectors of several disease are discussed.
PENGARUH SPESIES BAKTERI DAN RATIO SPERMATOZOA/BAKTERI TERHADAP VITALITAS SPERMATOZOA MANUSIA SECARA IN VITRO Sukarjati Sukarjati; Hamdani Lunardhi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/508

Abstract

The effect of some pecies of bacteria and sperm/bacteria ratio on sperm vitality has been studied. Four species of bacteria were used in this study: Stapylococcus epidermidis, Streptococcus faecalis, Enterobacter aerogenes were obtained from semen culture of infertile men and E. coli was obtained from prostaltic fluid culture from men with prostat and urinary system disturbances. Five semen samples fulfilling the WHO criteri (1992) were used in this study. After preparation by Percoll gradient-column method, sperm were inoculated in a microplate with Stapylococcus epidermidis, Streptococcus faecalis, Enterobacter aerogenes and E. coli under the sperm/bacteria ratio 1:10 and 1:1000. Sperm vitality was observed immediately, 3 and 6 hours after inoculation. At the second experiment, the detrimental influence of bacteria on sperm was prevented by adding penicillin. Result of this study indicated that Stapylococcus epidermidis, Streptococcus faecalis, and Enterobacter aerogenes were not affected on the sperm vitality. The effect of E. coli on sperm vitality occurred at the ratio of sperm/bacteria 1:10 after 3 and 6 hours incubation and at the ratio of sperm/bacteria 1:1000 occurred after 6 hours incubation. It might be concluded that the negative influence of bacteria on sperm vitality in vitro, is dependent in species of bacteria, bacteria concentration, and time of incubation. The most detrimental effect on sperm vitality was shown by E. coli at the ratio of sperm/bacteria 1:10 after 6 hours incubation. The detrimental effecr was not prevented bt the addition of penicillin.
PENGARUH PEMUPUKAN DAN VEGETASI TERHADAP KEBERADAAN JAMUR TANAH DI LAHAN BEKAS PENAMBANGAN EMAS YANG DIREKLAMASI PADA DAERAH CIMANGGU DAN BOJONG PARI JAMPANG SUKABUMI Titin Yulinaeri; Suciatmih Suciatmih; Nandang Suharna
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.418 KB) | DOI: 10.23869/509

Abstract

In order to know the effect of vegetation and fertilizer on the occurrence of soil fungi, a study was conducted in reclamated gold mining land both in Cimanggu and Bojong Pari, Jampang, Sukabumi. Soil fungi were isolated by dilution plate method, using a “tauge sucrose agar� medium with 50 ppm antibiotic and incubated at room temperature for 2-3 days. Fungi identification follows Domsch et al (1980), Samson et al (1981), and Barnet (1969).The effect of vegetation and fertilizer on the composition of soil fungi in reclamated land both Cimanggu and Bojong Pari were not quite different. Aspergillys, Eupenicillium, and Pennicillium maybe dominant fungi in these areas. These fungi were probably involved in reclamation of land. To obtain faster reclamation process, the fungi are better involved as one of introduced microorganism besides Rhizobium and Mychorizal fungi or mixed inoculants. Besides election of suitable flora and fauna, soil fungi are expected to be able recover degraded land into original ecosystem.
KEMAMPUAN TUMBUH Ecoli DH5 KOMPETEN DALAM 'MEDIUM MINIMAL' MENGANDUNG DEKSTRAN UNTUK MENGEMBANGKAN METODE SELEKSI KLON GEN DEKSTRANASE Afaf Baktir; Kuntaman Kuntaman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/510

Abstract

Dextranase gene cloning so far have used selection method base on halo formation around he recombinant dex colony grown on LB – blue dextran agar plate. The difficulty of the cloning process is in the selection of dex positive clone. As an example, for obtaining dex gene it has been screened about 36500 colonies. The reason that it was difficult to determine Dex positive clone because dextran hydrolysis by primary recombinant E.coli cells in LB – blue dextran medium was too weak. In the present research, we have designed a minimal medium contained dextran and low concentration of yeast extract to reduce difficulty and to increase accuracy and reproducibility determining of recombinant dex E.coli.In this experiment, dex positive cloned was simulated by competent E.coli grown in medium contained dextranase. The minimal medium designed consist of dextran 1% + yeast extract 0.01% +KH2PO4 0.1% + MgSO4 0.24% + NaCl 0.1% + CaCl2 0.01%. this medium have proved can distinguish between recombinant E.coli dex and other E.coli ie. The competent E.coli can grow well in this medium which was supplied by dextranase, but without dextranase this competent E.coli did not or limited grow

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