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All Journal Jurnal Veteriner Majalah Kedokteran Bandung Folia Medica Indonesiana Majalah Obstetri dan Ginekologi Syntax Literate : Jurnal Ilmiah Indonesia ISM (Intisari Sains Medis) : Jurnal Kedokteran Berkala Penelitian Hayati

Hamdani Lunardhi

Department Of Andrology, Faculty Of Medicine, Universitas Airlangga, Dr. Soetomo Hospital, Surabaya

Author-ID : 309784

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Density Gradient Centrifugation Pra-freezing Mengoptimalkan Persentase Morfologi Normal Spermatozoa Pasca-thawing Suyono, Seso Sulijaya; Hinting, Aucky; Lunardhi, Hamdani; I'tishom, Reny
Majalah Kedokteran Bandung Vol 50, No 3 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Kriopreservasi akan mengganggu struktur dan fungsi spermatozoa. Preparasi semen mampu menghasilkan spermatozoa dengan kualitas baik. Penelitian ini bertujuan menganalisis pengaruh preparasi semen dengan density gradient centrifugation (DGC) pra-freezing terhadap kualitas spermatozoa pasca-thawing. Penelitian dilakukan di boratorium Biologi Kedokteran, Fakultas Kedokteran Universitas Airlangga periode November 2017â€“Januari 2018. Penelitian eksperimental laboratoris dilakukan menggunakan cairan ejakulat volunter pria infertil. Semua sampel dibagi menjadi dua bagian, kelompok kontrol serta kelompok perlakuan berupa preparasi mini DGC. Setelah penambahan krioprotektan, selanjutnya dilakukan freezing. Pemeriksaan kualitas spermatozoa, meliputi motilitas, viabilitas serta persentase morfologi normal menggunakan metode WHO 2010, baik pra-freezing maupun pasca-thawing. Persentase perubahan kualitas spermatozoa pasca-thawing kedua kelompok dibandingkan menggunakan uji t dan bermakna jika nilai p<0,05. Total 20 sampel cairan ejakulat digunakan dalam penelitian ini. Persentase penurunan motilitas progresif, motilitas total, serta viabilitas pasca-thawing antara kedua kelompok tidak berbeda bermakna dengan nilai p masing-masing 0,422; 0,873 serta 0,432. Namun, penurunan persentase morfologi normal pasca-thawing pada kelompok kontrol jauh lebih besar daripada kelompok perlakuan dengan nilai p<0,001. Penelitian ini menyimpulkan bahwa preparasi semen berupa mini DGC pra-freezing mampu menghasilkan spermatozoa pasca-thawing dengan persentase morfologi normal yang lebih baik daripada protokol direct freezing.Pre-freezing Density Gradient Centrifugation Optimizes the Percentage of Post-thawed Sperms with Normal MorphologyÂ Cryopreservation impairs sperm structure and functions. Sperm preparation is a selection technique to obtain a population of high quality sperms. This study aimed to analyze the effect of pre-freezing Density Gradient Centrifugation (DGC) sperm preparation on the quality of post-thawed sperms. An experimental laboratory study was conducted using the ejaculates collected from volunteers visiting to our department. Samples were split into two fractions: control group and treatment group. In the treated group, mini-DGC sperm preparations were developed. After some cryoprotectants were added, samples were then cryopreserved using rapid freezing protocol. The evaluation of sperm quality that included evaluation on motility, viability and percentage of sperm morphology was performed by referring to 2010 WHO standardization on semen analysis. Evaluation was performed under pre-freezing and post-thawed condition. The percentage of sperm parameter changes between the two groups were compared using t-test with p-value <0.05 considered statistically significant. A total of 20 samples were included in this study. Post-thawed progressive motility, total motility, and viability considerably declined between two groups with p-values of 0.422, 0.873, and 0.432 respectively. In post-thawed observation, the percentage of sperms with normal morphology was significantly lower in the control group when compared to the treatment groupÂ (p<0.001). In conclusion, the pre-freezing mini-DGC can optimize the post-thawed percentage of sperms with normal morphology compared to direct freezing protocol

Hyaluronan Expression on Vitrified Oocytes Before and After In Vitro Maturation (EKSPRESI HYALURONAN PADA OOSIT YANG DIVITRIFIKASI SEBELUM DAN SESUDAH MATURASI IN VITRO) Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi; Widjiati Widjiati
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Oocyte vitrification is a major challenge in assisted reproductive technology. Oocyte vitrification with cumulus cells provide benefits in the process of maturation and fertilization. Vitrification leads to rapid temperature changes, therefore the decreasing in temperature could damage the cells even when the morphology was normal. Vitrification of mature oocytes is common because of its low sensitiveness towards low temperatures than immature oocytes. The aim of the research was to compare the effect of vitrification before and after in vitro maturation to the expression of hyaluronan. Maturation was operated in medium TC 50 ?L in CO2 incubators for 24 hours. Vitrification started with washing oocyte in PBS basic medium supplemented with 20% serum for 1-2 minutes, then in equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose (K1); 2) PBS + 20% serum + 0.25 M sucrose (K2); and 3).PBS + 20% serum + 0.1 M sucrose (K3). Immunocytochemical stain was performed to evaluate the hyaluronan expression. Remmele scale index (Immunoreactive score, IRS) was used to read the result. There was no differences of hyaluronan expression in oocyte and cumulus group of K1, K2 and K3 at p< 0.05, statistically. We concluded that there was no difference of hyaluronan expression on oocyte and cumulus between vitrified oocyte of pre and post in vitro maturation which indicated that oocyte could be vitrified in the immature and mature state.

Effect of oocyte vitrification before and after in vitro maturation towards Bcl-2, Bax and Bcl-2/Bax ratio expression Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi
Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August
Publisher : Universitas Airlangga

Objectives: to compare the expression of Bcl-2, Bax and Bcl-2/Bax ratio in cumulus cell and oocyte between vitrified oocyte pre and post in vitro maturation.Materials and Methods: Maturation was operated in medium TC 100 µl for 24 hours. Vitrification begins with washing oocyte in PBS basic medium supplemented of 20% serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Imunocytochemistry observed the expression of Bcl-2, bax and Bcl-2/bax ratio.Results: Bcl-2 expression on oocyte in control group differed significantly with treatment group, Bcl-2 expression on cumulus in control group differed significantly with treatment 1 group. Bax expression on oocyte in control group differed significantly with treatment group. Bax expression on cumulus in control group differed significantly with treatment group. Bcl-2/Bax expression ratio on oocyte and cumulus did not differ significantly in all groupConclusion: No difference Bcl-2/Bax expression ratio on oocyte and cumulus between vitrified oocyte pre and post in vitro maturation.

Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF) Audia Mumtaz Rifasky; Puspa Wardhani; Ashon Sa’adi; Ninik Darsini; Hamdani Lunardhi; Zakiyatul Faizah
Majalah Obstetri dan Ginekologi Vol. 29 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V29I12021.14-17

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia.

IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE Reny I’tishom; Doddy M Soebadi; Aucky Hinting; Hamdani Lunardhi; Rina Yudiwati
Folia Medica Indonesiana Vol. 52 No. 3 (2016): JULY - SEPTEMBER 2016
Publisher : Faculty of Medicine, Universitas Airlangga

One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.

Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process Gede Wira Buanayuda; Hamdani Lunardhi; Indra Gusti Mansur
Folia Medica Indonesiana Vol. 55 No. 4 (2019): December
Publisher : Universitas Airlangga

Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a control group, (k2) sperm preparation group without ALA, (k3) group of sperm preparation with the addition of ALA. The motility of spermatozoa was observed with the WHO 1999 method for 4 hours in units of percent. Progressive motility in k3 (47.95 ± 3.617) was higher than in k2 (38.05 ± 3.278) statistically significantly different after 3 hours of observation (p<0.0001). Progressive motility in k3 (78.8 ± 5.841) was higher than k1 (56.55 ± 7.511) from the initial observation (p <0.0001). The progressive motility of k2 (76.05 ± 6.768) was higher than k1 (56.55 ± 7.511) from the start of the observation (0.0001). It can be concluded that the addition of ALA in the sperm preparation process increases and maintains progressive motility that is better than sperm preparation without ALA addition after 3 hours of observation.

Differences of Indirect MAR Test in the Unexplained Infertile and Fertile Couples Nur Hafiz Ramadhona; Aucky Hinting; Hamdani Lunardhi
Folia Medica Indonesiana Vol. 56 No. 1 (2020): March
Publisher : Universitas Airlangga

Infertility is the inability of a partner to get pregnant after regular sexual intercourse without contraception for 12 months. Based on the cause, male infertility is caused by genetic, hormonal, infectious, sexual, and unexplained infertility. However, many infertile couples are classified into unexplained infertility without an antisperm antibody (ASA) immunological examination. This study aimed to prove that couples classified as unexplained infertility can be caused by ASA wives. This was an observational study with a cross sectional study design. The sample consisted of 2 groups, in which the first group consisted of 36 fertile couples and the second group consisted of 35 unexplained infertility couples. All samples were carried out indirect MAR test to obtain the ASA value of the wife. If the wife's ASA value was >40%, the ASA results were positive. Conversely, if the ASA value was <40%, the ASA results were negative. From these 2 groups, there was a statistically significant difference (p<0.0001) that the wife's ASA value in the unexplained infertility group was significantly higher than that in the fertile group. This study concluded that the positive ASA results from the indirect MAR test were significantly found in the unexplained infertility sample.

PENGARUH SPESIES BAKTERI DAN RATIO SPERMATOZOA/BAKTERI TERHADAP VITALITAS SPERMATOZOA MANUSIA SECARA IN VITRO Sukarjati Sukarjati; Hamdani Lunardhi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/508

The effect of some pecies of bacteria and sperm/bacteria ratio on sperm vitality has been studied. Four species of bacteria were used in this study: Stapylococcus epidermidis, Streptococcus faecalis, Enterobacter aerogenes were obtained from semen culture of infertile men and E. coli was obtained from prostaltic fluid culture from men with prostat and urinary system disturbances. Five semen samples fulfilling the WHO criteri (1992) were used in this study. After preparation by Percoll gradient-column method, sperm were inoculated in a microplate with Stapylococcus epidermidis, Streptococcus faecalis, Enterobacter aerogenes and E. coli under the sperm/bacteria ratio 1:10 and 1:1000. Sperm vitality was observed immediately, 3 and 6 hours after inoculation. At the second experiment, the detrimental influence of bacteria on sperm was prevented by adding penicillin. Result of this study indicated that Stapylococcus epidermidis, Streptococcus faecalis, and Enterobacter aerogenes were not affected on the sperm vitality. The effect of E. coli on sperm vitality occurred at the ratio of sperm/bacteria 1:10 after 3 and 6 hours incubation and at the ratio of sperm/bacteria 1:1000 occurred after 6 hours incubation. It might be concluded that the negative influence of bacteria on sperm vitality in vitro, is dependent in species of bacteria, bacteria concentration, and time of incubation. The most detrimental effect on sperm vitality was shown by E. coli at the ratio of sperm/bacteria 1:10 after 6 hours incubation. The detrimental effecr was not prevented bt the addition of penicillin.

Factors Affecting The Outcome of in Vitro Fertilization (IVF) Fayka Putri Poempida; Jimmy Yanuar; Hamdani Lunardhi; Samsulhadi Samsulhadi; Relly Y. Primariawan
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

The high prevalence of infertility motivated researchers to find a solution, henceforth In Vitro Fertilization was invented. Factors that affect the outcome of IVF may include sperm analysis, maternal Body Mass Index (BMI), maternal smoking habits, endometriosis, and maternal age. However, there are ongoing debates about the role of said factors regarding the outcome of IVF. The objective of this research is to analyze those factors. This research is a Case-Control study with an analytical observational design. Data were retrieved from patients’ medical records undergoing IVF in Graha Amerta Fertility Clinic from January 2019-October 2020. First, the Chi-Square Test revealed sperm abnormality (p=0.212), Maternal BMI (p=0.427), endometriosis (p=0.067), meaning there was no connection with the outcome of IVF. Simultaneously, maternal age (p=0.037) showed a connection with the outcome of IVF. From the Binary Logistic Regression Test, maternal age 36-40 years old (p=0.044) affects the outcome of IVF significantly. Concurrently maternal BMI, endometriosis, and sperm abnormality have p value>0.05 meaning it is insignificant to the outcome of IVF. This research concluded that sperm abnormality, maternal BMI, and endometriosis do not affect the outcome of IVF. There was no data about maternal smoking habits. Whilst maternal age affects the outcome of IVF. Conclusion: This research concluded that sperm abnormality, maternal BMI, and endometriosis do not affect the outcome of IVF. There was no data about maternal smoking habits. Whilst maternal age affects the outcome of IVF.

Hubungan Usia Ibu, Jumlah AFC, dan Total Pemberian Gonadotropin terhadap Kualitas Embrio pada Program Fertilisasi In Vitro di Klinik Fertilitas Graha Amerta RSUD Dr. Soetomo Surabaya Periode 2018 – 2019 Chitra Devi Paramita Prasetyawati; Budi Santoso; Hamdani Lunardhi; Hendy Herdarto; Ashon Sa’ad
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

Fertilisasi In Vitro merupakan metode untuk mendapatkan kehamilan pada pasangan infertil. Meskipun dengan FIV, angka keberhasilan kehamilan masih cukup rendah yang tentu tidak terlepas dari pengaruh kualitas embrio yang didapatkan. Banyak faktor yang dapat mempengaruhinya, beberapa contohnya adalah usia ibu, jumlah AFC, dan total pemberian gonadotropin. Namun, penelitian mengenai ini masih terbatas terkait literatur dan informasinya. Mengetahui hubungan usia ibu, jumlah AFC, dan total gonadotropin terhadap kualitas embrio pada program FIV di Klinik Fertilitas Graha Amerta Periode 2018- 2019. Penelitian ini menggunakan rancangan cross sectional dengan metode total sampling. Bahan penelitian dari rekam medis pasien yang menjalami program FIV di Klinik Fertilitas Graha Amerta periode 2018-2019. Dari 52 pasangan yang menjalani FIV didapatkan hasil p-value antara usia dengan kualitas embrio baik hari ke-3 0,020, degenerasi 0,003, kualitas embrio baik hari ke-5 0,007, buruk 0,048. AFC dengan kualitas embrio baik hari ke-3 0,000, degenerasi 0,001, kualitas embrio baik hari ke-5 0,001. Total gonadotropin dengan kualitas embrio hari ke-3 dan ke-5 <0,05. Biochemical pregnancy dengan kualitas embrio baik hari ke-3 0,013. Penelitian ini membuktikan bahwa usia ibu dan jumlah AFC berhubungan dengan kualitas embrio. Total pemberian gonadotropin tidak berhubungan dengan kualitas embrio. Namun, apabila diteliti secara bersamaan terdapat hasil pengaruh signifikan secara simultan antara usia ibu, jumlah AFC dan total pemberian gonadotropin terhadap persentase kualitas embrio baik pada hari ke-3 dan hari ke-5.

Co-Authors Amarudin Amarudin Ashon Sa’ad Ashon Sa’adi Aucky Hinting Aucky Hinting Aucky Hinting Audia Mumtaz Rifasky Ayang Halim Budi Santoso Chitra Devi Paramita Prasetyawati Doddy M Soebadi Faizah, Zakiyatul Fayka Putri Poempida Gede Wira Buanayuda Ginting, Aucky Hendy Herdarto I'tishom, Reny Indra Gusti Mansur Indra Gusti Mansur Jimmy Yanuar Lutfi Hardiyanto Mansur, Indra Gusti Ninik Darsini Nur Hafiz Ramadhona Puspa Wardhani R. Haryanto Aswin R. Haryanto Aswin Ramadhona, Nur Hafiz Relly Y. Primariawan Rina Yudiwati Samsulhadi Samsulhadi Sukarjati Sukarjati Supardi Supardi Supardi Supardi Suyono, Seso Sulijaya Wahyuning Ramelan Widjiati Widjiati

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