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Farhan Baehaki
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Asian Journal of Health and Applied Sciences
Published by Lighthouse Publishing Company
ISSN : -     EISSN : 28298985     DOI : https://doi.org/10.53402/ajhas
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health
Asian Journal of Health and Applied Sciences (AJHAS) is an open access journal publishing high quality peer-reviewed articles across the breadth of health and applied sciences research. The journal takes an objective and constructive approach to peer-review, ensuring each manuscript is well evaluated on its own scholarly merits and research integrity. The journal considers research articles and literature reviews in the areas of medicine, nursing, midwifery, pharmacy, medical laboratory technology (hematology, clinical chemistry, immunoserology, bacteriology, clinical toxicology, immunohematology, cytotechnology, histotechnology), medical records, nutrition science, chemistry (biochemistry, health instrumentation, analytical chemistry, environmental chemistry, organic chemistry), biology (microbiology, molecular biology), occupational health and safety in the health sector, and public health.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)" : 5 Documents clear
The Effect of Different Extraction Temperatures of SARS-COV2 RT-PCR Examination Process On CT Value In Patient Specimens In Labkesda Kabupaten Sukabumi, Indonesia Fadhilah, Fitri Rahmi; Wahid, Aziz Ansori; Rachman, Mochamad Irwan Fitriana
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.39

Abstract

The Severe Acute Respiratory Syndrome Coronavirus-2, or COVID, was announced by WHO on February 11, 2020.  Examination of SARS-CoV2 by RTq-PCR method in Labkesda Kab. Sukabumi is one of the health service support units in the Sukabumi area. The number of PCR examination requests is increasing every day, whereas, at this stage, the temperature increase process should reach the recommended temperature of 90°C, which can take 20-25 minutes. Sometimes ATLM has processed specimens at 60°C - 90°C. Given the required temperature at the time of extraction, which is at a temperature of 90 degrees, here the researchers wanted to prove whether below 90 degrees had an effect on the virus extraction process. This study aims to determine the effect of differences in extraction temperature on the SARS-CoV2 RTq-PCR examination process on CT VALUE on patient specimens. The variable in this study was the difference in temperature consisting of 60°C, 80°C, and 90°C. This research used quantitative research methods with case studies. This study is an experimental study to determine the effect of temperature differences on the CT value of the RTq - PCR examination. The population of this study were 30 patients at LABKESDA Kabupaten Sukabumi. This analysis used a one-way ANOVA test analysis with software. IBM SPSS 23.0. It may be concluded that there is no significant difference in the CT Value findings on the SARS-CoV2 RTq-PCR Examination since the study's p-value was 0.758, which is more than 0.05.
Test of The Inhibitory Power of Lemongrass Extract Against The Growth of Candida albicans On Natural Resources Medium Murtafi'ah, Ni'matul; Syarofah, Zela Adeline; Pitono, Arie J.
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.59

Abstract

Candidiasis is an acute or chronic inflammation that causes disorders of the body's system caused by the fungus Candida albicans. Candida albicans is a common cause that does not cause disease in a person's normal immune system but can attack a person's poor system. Candidiasis can be treated with antifungal drugs, but prolonged and extensive use of antifungal drugs causes side effects. So alternative drugs are needed that can reduce these side effects. Lemongrass (Cymbopogon citratus) has pharmacological activity as an antifungal. Research Objectives: To determine the inhibition of lemongrass extract on the growth of Candida albicans in an SDA medium with concentrations of 0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The study used a descriptive research design. The method of testing the resistance is carried out by the good diffusion method. Lemongrass extract can inhibit the growth of Candida albicans fungus by forming an inhibition zone. At a concentration of 0% lemongrass extract, no inhibition zone was formed, a concentration of 0.2% formed an inhibitory zone of 10.5 mm, a concentration of 0.4% formed an inhibition of 6.1 mm, a concentration of 0.6% formed an inhibition zone of 14 mm, the concentration 0.8% formed an inhibition zone of 10.8 mm, a concentration of 15 formed an inhibitory zone of 27.6 mm.
Identification of Trichomonas vaginalis Parasites In Commercial Sex Workers Conducting Examinations At The Mawar Clinic PKBI Bandung, Indonesia Kodariah, Liah; Nisa, Putri Khoerun; Pakpahan, Suyarta E.; Aulia, Syifa
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.82

Abstract

Trichomoniasis is a sexually transmitted infection (STI) caused by the pathogenic Trichomonas vaginalis protozoa parasite found in the female genitourinary tract and can cause vaginal discharge, in severe conditions, vaginal inflammation will occur. Trichomoniasis has a high risk of infecting groups that often have multiple partners, such as Commercial Sex Workers (CSWs). This study aims to determine the presence or absence of Trichomonas vaginalis parasites in the urine sample of Commercial Sex Workers who conduct examinations at the Mawar Clinic PKBI Bandung. The research method used is observation. The sample is the urine of a prostitute who performs an examination at the Mawar Clinic PKBI Bandung, Indonesia. The examination was carried out in two ways, namely the wet method and the Giemsa staining method, which was carried out in the Rajawali Bandung Institute of Health laboratory. The results showed that out of 30 samples, 11 urine samples of CSW were infected with Trichomonas vaginalis,. The conclusion of this study is that Trichomonas vaginalis parasites were found in urine samples of prostitutes who were examined at the Mawar Clinic PKBI Bandung.
Potential Bromelain Pinneapple Extract to Breaker Tempe Protein As Organic MSG Tias, Eriza Putri Ayu Ning; Wicaksono, M. Galih; Hayati, Lu'lu'ul Rosyiqul; Salsabila, Aulia Firda; Prabowo, Kus Alan Dhimas; Setyaningsih, Endang
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.182

Abstract

New alternatives are needed to change flavorings from chemicals to organic so that they do not have a bad impact on health. The study's results proved that giving MSG at a dose of 9 g / day to red blood cells caused the appearance of a micronucleus so that it indicated a mutagenic MSG. The potential of bromelin enzyme contained in pineapple fruit is able to break down proteins from tempe so that it can give rise to a savory taste that is generally found from other flavorings. The purpose of this study was to determine the potential of bromelin extract pineapple, which is a tempe protein breaker as an organic flavoring sourced from various literatures. The research was conducted using a literature review method of reference sources such as google scholar and research gate. A total of 40 articles were found and then selected based on predetermined parameters resulting in 25 selected articles. From the results of the literature search obtained a percentage of 80% for the sampling method on the extraction method, 16% centrifuged and the method using extraction and centrifugation 4%. Meanwhile, judging from the variables studied, 52% were obtained regarding the activity of hydrolyzing proteins, 12% for the characterization of the enzyme bromelin, 8% for the mechanism of action of bromelin as an anti-bacterial and 4% in the stacking of meat, catalyzing peptide bonds, mobilization of bromelin enzymes, meat quality, decreased glucose levels, healing of lukan and physical quality of cheese curd. And when viewed from the part of the pineapple plant that is utilized 44% of the flesh of the pineapple fruit, 12% of the pineapple weevil, 8% each of the flesh of the fruit and the fruit weevil of the fruit, the skin of the pineapple fruit and the flesh of the young pineapple fruit and 4% each of the ripe pineapple weevil, the flesh of the ripe fruit, the stem and the final leaf of the pineapple plant. And when viewed from the use of the benefits of bromelin, a score of 36% was obtained to increase protein levels in the product, 20% to speed up the hydrolysis process, 12% to stack meat, 8% to inhibit streptococcus bacteria, and 4% each was used to hydrolyze collagen. Helps the fermentation process, anti-browning agents fund therapeutic genes.
Analysis of The Heavy Metal Content of Zinc (Zn) In Tofu Using Atomic Absorption Spectrophotometer (AAS) Lutfi, Nadira Nur Hajah; Rizal, Fahmi
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.191

Abstract

Tofu, which is rich in protein, has long been consumed by the people of Indonesia as a side dish. Tofu is a food made from fermented soybeans and extracts from it. One of the metals that is dangerous and should not be eaten is zinc (Zn). Zinc is a type of heavy metal that is soft and easy to purify. This study aims to determine the content of heavy metal zinc (Zn) contained in tofu by using Atomic Absorption Spectrophotometry (AAS). The advantages of an Atomic Absorption Spectrophotometer (AAS) are specific, low detection limit, from the same solution, several different elements can be measured, can be applied to many types of elements in many types of samples, limit levels that can be determined are very area (mg/L to percent). This research uses a descriptive-analytical method to describe or provide an overview of the object under study through the data. And this research uses the wet destruction method, namely the destruction of the sample with the addition of a solution of nitric acid (HNO3) followed by heating and filtering. The results showed that the concentration of heavy metal Zn in tofu was 0.161 ppm, 0.139 ppm, 0.161 ppm, 0.152 ppm, 0.153 ppm, 0.154 ppm, 0.159 ppm, 0.174 ppm, 0.185 ppm, 0.141 ppm, 0.159 ppm and 0.164 ppm. The conclusion is that the levels of tofu Zn contain zinc metal Zn with levels of 10.3325 mg/kg. The levels of zinc metal Zn are still below the threshold level, so tofu is still safe for consumption.

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