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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 6 Documents
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Search results for , issue "Vol 2, No 2 (2022)" : 6 Documents clear
THE EFFECT OF DOSAGE AND TYPE OF FERTILIZER ON THE PERCENTAGE OF ATTACKS OF BROWN PLANTHOPPER (Nilaparvata lugens STALL.) ON SOME VARIETIES OF PADDY (Oryza sativa L.) Arkhiadi Benauli
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.62134

Abstract

The effect of dosage anf type of fertilizer on the percentage of attacks of brown planthopper (Nilaparvata lugens Stall.) on Some Varieties of Paddy (Oryza sativa L.). This study aims to determine the dose of cow manure on the percentage of brown plant hopper attack  in several varieties of paddy. This research was conducted in Desa Serdang in March-June 2021. This research used Randomized Block Design (RAK) of two factors and three replications. The first factor  is fertilizer dose consisting of 4 treatments (anorganic, 50 kg / plot, 100 kg / plot and 150 kg / plot). The other factor  is a variety consisting of 2 treatments (Inpari 10 and Inpari 32). The results showed that the dosage of 100 kg / plot cow manure was the best treatment to production per plot and resistant to the pecentage of brown plant hopper attack. Inpari 32 is the best treatment to increase production per plot and resistant to the intensity of brown plant hopper attack. The interaction of fertilizer dosage and variety (Inpari 32 + 100 kg / plot) is the best treatment to increase production per plot and resistant to the percentage of brown plant hopper attack.
Application Cow Manure and Mychorriza to Physiological of Biduri (Calotropis gigantea) Sri Hartati; Samanhudi Samanhudi; Muji Rahayu; Mochammad Danny Sukardan; Nadia Alifia Rahma
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.61273

Abstract

Biduri (Calotropis gigantea) was a wild plant that had many benefits in the health and textile industries. In the health sector, biduri was used as a herbal plant, while in the textile industry, used as the main raw material. Biduri could be used as a substitute for kapok as the main raw material, therefore it was necessary to develop cultivation technology to met the needs. This research was conducted at the Jumantono Field Laboratory of Faculty of Agriculture UNS, Karanganyar using a factorial Randomized Complete Block Design (RCBD), which consisted of 2 factors, namely cow dung fertilizer, and mycorrhizae. Cow dung fertilizer consists of 0 ton/ha, 10 ton/ha, and 20 ton/ha. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10gram/plant, and 15gram/plant. There were 36 treatment combination that consist of 12 treatment combination repeated 3 times. The interaction between cow dung did not significantly affect all physiological observation variables. The results showed that the application of 20 ton/ha of cow dung fertilizer gave the best results on the width of stomata openings, the rate of photosynthesis, and the rate of transpiration. Inoculation with mycorrhizal fungi had no significant effect on all observations of physiological variables Keywords: Calotropis gigantea, cow manure, mycorrhiza, physiological
Application of Livestock Waste Liquid Fertilizer and Mychorriza Biofertilizer on The Growth of Biduri (Calotropis gigantea) Muhammad Naufal Adani; Samanhudi Samanhudi; Amalia Tetrani Sakya; Sri Hartati; Muji Rahayu; Anne Sukmawati
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.63262

Abstract

Biduri (Calotropis gigantea) was a plant with several benefits, namely as a medicinal plant and textile material. Biduri was a wild plant that could grow in various types of soil conditions. The existence of biduri was still not fully utilized by many people. So it was necessary to develop the cultivation technology of Biduri to take advantage of this plant. This research was conducted at the field laboratory of Jumantono, Faculty of Agriculture, Universitas Sebelas Maret, Karanganyar, and used a factorial Randomized Complete Block Design (RCBD), which consisted of 2 factors, namely organic liquid fertilizer from livestock waste and mycorrhizae. Livestock waste liquid fertilizer consists of 40ml/kg soil cow urine, 40ml/kg soil goat urine, 40ml/kg soil rabbit urine, and 40ml/kg soil fish waste. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10gram/plant, and 15gram/plant. There were 20 treatment combinations repeated 3 times, so the total was 60 treatment combinations. The interaction between livestock waste liquid fertilizer and mycorrhizae did not significantly affect all the physiological observation variables. The results showed that the application of livestock waste liquid fertilizer had no significant effect on all observations of physiological variables. Giving mycorrhizae shows that it can increase the number of stomata and the width of stomata aperture.
Genetic Variability of Eggplant (Solanum Melongena L. 'Nasubi') Based on ISSR Markers and Phenotypic Characters Nabila Shafura; Annas Rabbani; Budi Setiadi Daryono
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.65874

Abstract

‘Nasubi’ eggplant seeds have occurred since 2018. In addition, plants that produce fruit phenotypic characters differ from those planted before the planting period in 2018. These three factors cause the decline in harvest rates and the current high increase in production costs. Observation of variations in phenotypic and molecular characters with ISSR molecular markers between seeds before 2018 and after 2018, became the first step for the research team to reveal information on the uniformity of phenotypic and molecular characters in eggplant ‘Nasubi’ from seeds purchased by farmers before 2018 and post-2018. The results of the molecular analysis with ISSR primary molecular markers namely UBC 809, UBC 815, IBC 880, UBC 888, and UBC 892, showed that the six samples had a high similarity index of 90% and resulted in a low polymorphism average of 12%. The results of the phenotypic analysis showed that eggplants from the seeds before 2018 and seeds after 2018 in this study had variations in the character of the leaf tip angle, leaf base, flower crown color, stamen color, young fruit color, fruit curve, and fruit tip shape.
Root Growth Response of Soybean Under Water Deficit Achmad Fatchul Aziez; Agung Prasetyo; Paiman Paiman
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.66465

Abstract

Roots are plant organs that function to absorb water and nutrients from the rhizosphere. If the soil is dry, the roots will be affected first. This study aims to know the response of soybean root to drought stress. This research was arranged in a randomized completely block design (RCBD) with two factors and three replications. The first factor was soil moisture content, which consisted of four levels, e.i., 100, 75, 50, and 25% field capacity. The second factor was the growth stage, which consisted of three kinds, e.i., the vegetative active, flowering time, and seed filling period. The results showed that the soil water content in below 75% field capacity decreased root length, root fresh weight, root dry weight, root volume, and increased shoot root ratio. The seed filling period was more sensitive to water deficiency than the active vegetative and flowering time. The study findings that soybean plants can grow well at 100% field capacity. The practical implication of planting soybeans use a soil moisture content of 100% field capacity.
ANTIBACTERIAL ACTIVITY AND TLC-BIOTOGRAPHY PROFILE OF THE ETHYL ACETAT FRACTION OF ASIAN PIGEONWINGS FLOWER (Clitoria ternatea) AGAINST Escherichia coli Ari Susilowati; Rizkya Suhardo Putri; Widya Mudyantini
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.66769

Abstract

Infectious diseases are diseases caused by the presence of microorganisms that enter and develop in the body, causing several clinical symptoms and signs. One of the infectious diseases is diarrhea caused by Escherichia coli. There are many potential herbal plants as alternative antibacterial antibiotics, one of which comes from the Asian pigeonwings flower (Clitoria ternatea). This study aims to determine the antibacterial activity of the ethyl acetate fraction of pigeonwings flower, phytochemical compounds and its TLC-bioautography profile. The antibacterial compound of pigeonwings flower was extracted by maceration using ethanol and then fractionated by ethyl acetate and made solutions with concentrations of 5, 10, 20, and 40%, respectively. The experiment was carried out with 3 replications using the well diffusion method and followed by TLC-bioautography to determine the TLC-bioautography profile. Phytochemical compounds of pigeonwings flower were determined by thin layer chromatography (TLC). Antibacterial activity data were analyzed using the One Way ANOVA and continued with the Tukey HSD 5% through SPSS. The ethyl acetate fraction of pigeonwings flower had the best antibacterial activity at a concentration of 40% with an inhibition zone diameter of 11.54 mm. The diameter of the inhibition zone showed significantly different in each concentration and it can be said that the ethyl acetate fraction of pigeonwings flower was able to inhibit the growth of E. coli. The phytochemical compounds contained in the ethyl acetate fraction of pigeonwings flower are flavonoids and alkaloids with flavonoids being the most active compounds in inhibiting the growth of E. coli.

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