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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
Arjuna Subject : -
Articles 12 Documents
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Search results for , issue "Vol 4, No 2 (2014): Life Sciences" : 12 Documents clear
LOSE WEIGHT AND PREVENT OBESITY BY AEROBIC GYMNASTICS . Yusni
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Aerobic gymnastics is a sport that is very popular in today's society, especially for those who want to lose or control body weight. But sports alone is not enough to lose weight and it must be coupled with good eating pattern settings. The problem is many of us are very difficult to control our diet and it needs to be analyzed further. This research aims to carry out an analysis of the influence of aerobic gymnastics to weight loss without diet interventions given.  The subject was 15 women, aged 20-25 years old, have a normal body weight. The intervention provided in the form of gymnastic aerobics three times a week, performed for 45-minutes practice session, and each was given for 1 month. This research was a quasi experimental research with one group pretest-posttest design. Weight measurement was done before and after aerobic gymnastics given. The results indicated that the aerobic gymnastics for 1 month losing 0.73% of body  weight, but the decline was not statistically meaningful. This showed that aerobic gymnastics for 1 month for women in the age 20-25 years has started to give response, but the duration has not been enough to lose significantly of body weight. It was concluded that in order to lose or control body weight, besides  takes aerobic gymnastics with the duration of up to 1 month, it has to accompany with  diet arrangement. This would give effect to  prevent  overweight and obesity
FORMULATION OF TRICHODERMA VIRENS ORIGIN OF ACEH COCOA CONTROLLING BLACK POD DISEASE CAUSED BY PHYTOPHTORA PALMIVORA TJUT CHAMZURNI; RINA SRIWATI; RIZKY MUARIF; BUNI AMIN; ABDUH ULIM
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Trichoderma virens have been isolated from rooting cocoa in Aceh and in the laboratory have been reported capable of inhibiting the growth of P. palmivora in vitro. However, the formulations of T. virens from Aceh can be used as an alternative product for farmers in controlling black pod disease of cocoa. This research was conducted t the Laboratory of Plant Pathology College of Agriculture Syiah Kuala University and cocoa fruit samples obtained from cocoa plantations owned by farmers in Pidie Jaya District of Aceh. The purpose of the study was to obtain an application formulations strains of T. virensfrom Aceh, as one of the biological control study consisted of seven treatments, namely W (water), Pest (Mancozeb Fungicide), Det (Detergent), Det+Tv( detergent+strainsof T.virens), P+Tv (Adhesive +strainsof T.virens), Co+Tv( Corn Oil+strainsof T.virens), Po+Tv (Palm oil+strainsof T.virens) and each treatment was repeated 10 times to obtain70 experimental units. The parameters observed incubation period, disease incidence (%), and the diameter of the spots (cm). Application results show that the strains of T. virens Origin Aceh effective in controlling P. palmivora on cocoa pods and the best  formulation in controlling P. palmivora on cocoa pods are corn oil+T. virens formulation (Co +Tv) compared with other formulations are characterized by the length of the incubation period, the low incidence of P. palmivora disease, and the small diameter of the spots on the cocoa pods.
THE DIVERSITY AND DISTRIBUTION OF SEAGRASS IN KARANG TIRTA BEACH PADANG CITY, WEST SUMATERA ARIEF A. PURNAMA; INDRA J. ZAKARIA; JABANG NURDIN
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Diversity and Distribution of Seagrass in Karang Tirta Beach Padang City, West Sumatera was conducted from April to June 2011. This study was intended to analyze the diversity, distribution pattern, coverage, composition and structure community of seagrass in Karang Tirta beach. Measurement of distribution aspect was analyzed with line transect method and sample of seagrass collected by using squares plot 0.5 x 0.5 m. Approximately 12 ha total of seagrass was estimated in various areas, such as: intertidal zone of tourism area, people settlement and mangrove zone. Seagrass distribution pattern was grouping category, and it was found 2 of 13 Species from Family Hidrocharitaceae of Indonesian seagrass exist, they were Thalassia hemprichii about 1.59 and Enhalus acoroides about 9.95. They were included into poor seagrass category with coverage ranged between 21.11% for T. hemprichii and 5.66% for E. acoroides. The highest species density was T. hemprichii (309.2 ind/m2) with appearance frequency value 100% and important value 252. The lowest species density was E. acoroides (7.73 ind/m2) with appearance frequency value 33.33% and important value 48. 
METHYLCOBALAMIN EFFECT ON MECHANICAL BEHAVIOR ALLODYNIA IN RATS SPRAGUE DAWLEY ENDANG MUTIAWATI
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Neuropathic pain is an expression of the nerve damage that impaired its excitability, such as increased excitability in the nerve lesion and surrounding healthy nerves as well. It is estimated that the incidence of neuropathic pain ranges between 2-40% of all adult men. Some neuropathic pain can not be cured, such as in patients with diabetes mellitus, trigeminal neuralgia, cervical syndrome, carpal tunnel syndrome, lumbar stenosis, herniated nucleus pulposus and the carcinoma. The pathophysiology of neuropathic pain are complex and not fully understood. Methylcobalamin is a metabolite of vitamin B12 acts as a coenzyme in the formation of methionine from homocysteine. This reaction is useful in the formation of DNA, as well as maintenance of nerve function. Through the methylation reaction, methylcobalamin also plays a role in the formation of lecithin, a protein that plays an important role in the regeneration of peripheral nerves, including the formation of myelin. This study aimed to determine animal mechanical alodinia with or without the administration of methylcobalamin. Twenty male Sprague Dawley rats, aged 2 months, with an average weight of 150-250 g, obtained from the Laboratory LPPT Gadjah Mada University, Yogyakarta. The mice were randomly divided into 4 groups, each consisting of 5 mice. All the rats, their fifth lumbar nerve were ligated. Group I was the control mice (C) 0.9% NaCl given, group II (M1) were given a dose of 50 microg methylcobalamin, group III (M2) were given a dose of 100 microg methylcobalamin, while group IV (M3) were given a dose of 150 microg methylcobalamin 13 weeks provision. For 13 weeks, neuropathic pain behavior was assessed. Neuropathic pain behavior in experimental animals observed about onset, duration and filaments Von Frey numbers that cause mechanical alodinia. Result of the study showed methylcobalamin influence the mechanical alodinia. Based on the results obtained, it is concluded that there was a reduction in neuropathic pain in the methylcobalamin group compared with the control group.
IL-12 PE, CD 69 PERCP, CD3 FITC, AND CD4 APC OPTIMIZATION WITH ACTIVATION OF ISOLATED AGENT HEAT-KILLED SONICATED MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN RINI SUNDARI; IDA PARWATI; JOHANES C. MOSE; BUDI SETIABUDIAWAN
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Infection caused by Mycobaterium tuberculosis exists in form of intracellular infection, which leads to lymphocyte activation. CD69 is the first lymphocyte activation marker expressed in Th1 lymphocyte, which follows by IL-12 release. Flow cytometry analysis can identify the subpopulations of lymphocytes and  intracellular cytokines such as IL-12, yet precise preparation needs to be done. This research aims to conduct optimization with four color lyse/wash flow cytometry assay system FastImmune™ FACSCalibur examination, with monoclonal antibody IL-12, CD69, CD3, and CD4 in succession uses fluorochrome PE, PerCP, FITC, and APC.To activate the lymphocytes from heparinized whole blood, we used activation agent which derives from isolated heat-killed sonicated Mycobacterium tuberculosis Beijing strain. Optimal concentration from the according activation agents is 40 mL. To determine the compensation, BDTM CompBead and blank-cell unstainning are used, but the maximum result showed by blank-cell unstainning.Each monoclonal antibody dosage of IL-12PE, CD69 PerCP, and CD3 FITC is 40 mL, while CD4 APC 5 mL. Total event lymphocyte is determined minimally by 10,000 events. With 18,510 total events and Th gated events quantity are 4,692, the result obtained is IL12-PE has 7.4% gated (347 events); CD69+ perCP/CD3+ FITC 18.2% (850 events); and CD69+ perCP/CD4+ APC 3.9%.
EFFECT OF BIOCHAR RESIDUE, COMPOST, AND UREA COMBINATION ON GROWTH AND YIELD OF MAIZE (ZEA MAYS L.) MOHD. CHALISZAR; . ZAITUN; E. NURAHMI
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The objective of this study was to know the effect of biochar residue, compost and urea fertilization on growth and yield of maize.  The research was conducted at University Farm Ie Seum Research Station, Aceh Besar district, Aceh Province, Indonesia.  The experimental arranged in a randomized complate block non factorial design with four replications.  There were eight treatment combinations: P1 (without biochar residue + without compost + without urea), P2 (without biochar residue + without compost + urea), P3 (without biochar residue + compost + without urea), P4 (without biochar residue + compost + urea), P5 (biochar residue + without compost + without urea), P6 (biochar residue + without compost + urea), P7 (biochar residue + compost + without urea), P8 (biochar residue + compost + urea).  Based on the plant growth, biochar residue, compost, and urea fertilizer treatment did not significantly affect on plant height age of 30, 45 and 60 days after planting, leaf number aged 30, 45 and 60 days after planting, stem diameter ages 30, 45 and 60 days after planting. At the P7 treatment (biochar residue + compost + without urea) gave the best value but does not differ significantly with all treatments tested.  Based on plant yield, biochar residue, compost, and urea fertilizer treatment did not significantly affect on cornhusk ear length, cornhusk cob diameter, cornhusk cob weight, cob length without cornhusk, cob diameter without cornhusk, cob weight without cornhusk, and yield.  At P5 treatment (biochar residue + without compost + without urea) cornhusk cobs and P7 (biochar residue + compost + without urea) cornhusk cobs and without cornhusk provide the best value but does not differ significantly with all treatments tested. 
BIOSYSTEMATICS OF HOSPITALITERMES HOSPITALIS HOLMGREN (ISOPTERA) FROM BORNEO . Syaukani
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This article redescribes Hospitalitermes hospitalis of open-air processional column termitesfrom Central Kalimantan, Borneo Indonesia.  In many publications, this nasute termite is one of very incomplete descriptions. Condition of head capsule and its coloration (soldier caste), mandibles and antennae (soldier caste) are importance characters identification work. This species showed a large variation of nesting sites and dimorphism of worker caste. 
THE CORRELATION BETWEEN DIETARY PATTERNS OF FOOD ANIMAL ORIGIN AND RISK OF BREAST CANCER IN BANDA ACEH EVA FITRIYANINGSIH; . NURLIANA; UMMU BALQIS
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The research was aimed to investigate the correlationbetween food animal origin dietary patterns and breast cancer in Banda Aceh. This case-control study compared the dietary pattern of food animal origin  between 45 breast cancer patients and 45 age-matched controls. Six  dietary patterns were difined by food frequency questioner(FFQ): food animal origin, preserved of food animal origin, processing of food, fats andoils, vegetables and fruits patterns. Preserved of food animal origin were significantly associated with the incidence of breast cancer with an odds ratio (OR) 5.86 [95% confidence interval (CI) =1.49-21.65] respectively; p=0.013; while food animal origin, processing of food, fats and oils, vegetables and fruits dietary patterns were not associated with the incidence of breast cancer. The conclusion of the research that salt fish and keumamah  play an important role of the association
EFFECT OF BIOCHAR RESIDUE, COMPOST, AND UREA COMBINATION ON GROWTH AND YIELD OF MAIZE (ZEA MAYS L.) MOHD. CHALISZAR; . ZAITUN; E. NURAHMI
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.216 KB)

Abstract

The objective of this study was to know the effect of biochar residue, compost and urea fertilization on growth and yield of maize.  The research was conducted at University Farm Ie Seum Research Station, Aceh Besar district, Aceh Province, Indonesia.  The experimental arranged in a randomized complate block non factorial design with four replications.  There were eight treatment combinations: P1 (without biochar residue + without compost + without urea), P2 (without biochar residue + without compost + urea), P3 (without biochar residue + compost + without urea), P4 (without biochar residue + compost + urea), P5 (biochar residue + without compost + without urea), P6 (biochar residue + without compost + urea), P7 (biochar residue + compost + without urea), P8 (biochar residue + compost + urea).  Based on the plant growth, biochar residue, compost, and urea fertilizer treatment did not significantly affect on plant height age of 30, 45 and 60 days after planting, leaf number aged 30, 45 and 60 days after planting, stem diameter ages 30, 45 and 60 days after planting. At the P7 treatment (biochar residue + compost + without urea) gave the best value but does not differ significantly with all treatments tested.  Based on plant yield, biochar residue, compost, and urea fertilizer treatment did not significantly affect on cornhusk ear length, cornhusk cob diameter, cornhusk cob weight, cob length without cornhusk, cob diameter without cornhusk, cob weight without cornhusk, and yield.  At P5 treatment (biochar residue + without compost + without urea) cornhusk cobs and P7 (biochar residue + compost + without urea) cornhusk cobs and without cornhusk provide the best value but does not differ significantly with all treatments tested. 
PREPARATION OF ANTI-IDIOTYPE ANTIBODY AS DIAGNOSTIC KIT IN RABIES SEROLOGICAL TEST SAYU P.Y. PARYATI; EKA N. NAWANGSIH; IIS I. RAKHMAT
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Serology remains the only way to monitor the effectiveness of vaccination of humans and animals against rabies. Many techniques for determining the level of rabies antibodies have been described, and all of the methods used rabies viruses as antigen. Indeed attenuated viruses can revert to a more virulent form, and inactivated virus may produce serious side effects. Anti-idiotype Ab can induce protective immune response against rabies virus, its means that anti-idiotype Ab can be used as surrogate antigen in serological test.The aim of this study is to prepare the anti-idiotype antibody (anti-idiotype Ab) as diagnostic kit in rabies serological test. Polyclonal anti-idiotype Ab were prepared in laying chickens and purified using affinity chromatography column for IgY. Rabbit anti dog immunoglobuline were prepared used New Zeeland White strain, and dog anti rabies serum were prepared from rabies immunized dog. For the preparation of the kit, it takes a few stages, i.e. the making of stocks A, stocks B and prototype diagnostic kit. Stocks A is a mixture of S. aureus Cowan I intact in solution of Tris Buffer HCl with rabbit anti dog serum in various comparisons (v/v). Stocks B is a mixture of rabies anti-idiotype Ab with rabies antibody (IgG anti rabies) are harvested from dog.Comparison of the obtained between whole S. aureus Cowan I and rabbit serum anti dog is 4~6:2~4. Optimization of stocks A and B based on the principle as follows: a merger of stocks A and B must not cause coagglutination. Formulation of aqueous stocks A and B will be a candidate when the diagnostic kits on the positive control produces coagglutination and negative controls do not produce coagglutination. The conclusions of this study is the anti-idiotype antibody can used and prepared as kit diagnostic with the principle of coagglutination by utilizing A protein of S. aureus.

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