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All Journal MAKARA of Health Series Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Althea Medical Journal Medical Journal of Indonesia Global Medical and Health Communication The Indonesian Biomedical Journal Sari Pediatri Journal of Medicine and Health Majalah Kedokteran Andalas Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Neuroanestesi Indonesia
Ida Parwati
Department Of Clinical Pathology, Faculty Of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung
Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Comparison of Estimated Glomerular Filtration Rate Mean Value of HARUS 15-30-60, HADI, and ASIAN Fomula Accuracy in Diabetes Mellitus Type 2 Rachmayati, Sylvia; Parwati, Ida; Martakusumah, Abdul Hadi; Soelaeman, Rachmat; Sekarwana, Nanan
International Journal of Integrated Health Sciences Vol 3, No 1 (2015)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: To compare the accuracy of HARUS 15-30-60, HADI, and Asian Formulas (Chinese-equation (Ch-E), Japanese-equation (Jp-E), and Thai- equation (Th-E)) for estimated glomerular filtration rate (eGFR).Methods: The Kidney Dialysis Outcome Quality Initiative (KDOQI) has published a guideline to measure renal function, which is based on glomerular filtration rate (GFR). This procedure is complicated and expensive, therefore an estimated GFR (eGFR) has been proposed. The modification of diet in renal disease (MDRD) study prediction equation is the most frequently eGFR used. This method still have a weakness in accuracy, so the chronic kidney disease epidemiology collaboration (CKD-EPI) formula is developed. Since CKD-EPI is not practical for daily use, the MDRD is published for Asian population that includes Ch-E, Jp-E, and Th-E. In Indonesia, the MDRD formula has not been validated using any gold standard, therefore 2 new formulas have been developed, i.e. HARUS 15-30-60 and HADI formulas. In this study, we analyzed 102 medical records of Diabetes Mellitus Type 2 (DMT2) patients who visited Dr. Hasan Sadikin General Hospital, Bandung during the period of 2012 to 2013. We analyzed the data using HARUS 15-30-60, HADI, Asian formulas, and then compared them to CKD-EPI to see the accuracy. Statistical analysis used was paired t-test in SPSS-17 program.Results: The accuracy of the different formulas are as follows: HADI (p=0.173), HARUS 15-30-60 (p=0.060), Ch-E (p=0.001), Th-E (p=0.000), and Jp-E (p=0.000).Conclusions: HADI is the most accurate formula, followed by HARUS Formula, Ch-E, and Th-E and Jp-E, respectively.Keywords: Chinese-equation, HADI and HARUS 15-30-60 formulas, Japanese-equation, and Thai-equation DOI: 10.15850/ijihs.v3n1.403
Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia van Soolingen, Dick; van Crevel, Reinout; Parwati, Ida; Alisjahbana, Bachti; Sudiro, Tjahjani M.; Rosana, Yeva
Medical Journal of Indonesia Vol 14, No 3 (2005): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.775 KB) | DOI: 10.13181/mji.v14i3.186

Abstract

At present, there is no standardized method for Mycobacterium tuberculosis drug susceptibility testing (DST) among laboratories in Indonesia. Since January 2001 to January 2004 we have tried to establish the method of 25-well culture plates with middlebrook’s media (Drug Susceptibility Culture Plate (DSCP) method) used by the Dutch Supranational Reference Laboratory at the Institute of Public Health and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that this method potentially gives better result as it can be very well standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration) values. Data from 364 isolates that have been tested by DSCP method showed that resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%, 15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2% isolates. (Med J Indones 2005; 14: 142-6)Keywords: M. tuberculosis, DST, DSCP method
Efficiency of BacT/Alert in detecting Salmonella Samaun, Endang; Parwati, Ida
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

[no abstract available]
Multidrug resistance Salmonella in Dr. Hasan Sadikin General Hospital, Bandung Samaun, Endang; Parwati, Ida
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

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no abstract available
Uji Validitas Neutrophil Gelatinase Associated Lipocalin sebagai Penanda Diagnosis Gangguan Ginjal Akut pada Sepsis Hidayat, -; Parwati, Ida; Gondodiputro, Rubin Surachno; Rita, Coriejati
Majalah Kedokteran Bandung Vol 44, No 2 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Gangguan ginjal akut (GgGA) merupakan penurunan fungsi ginjal secara mendadak yang ditandai dengan peningkatan kreatinin serum ≥0,3 mg/dL atau meningkat >1,5 kali dari kadar sebelumnya atau penurunan urine output (UO) <0,5 mL per jam selama >6 jam. Sepsis merupakan penyebab tersering GgGA dengan angka kejadian berkisar 20–50% dan angka kematian mendekati 70%. Kadar neutrophil gelatinase associated lipocalin (NGAL) urine penderita GgGA dapat meningkat secara cepat dan lebih awal dibandingkan dengan kadar kreatinin serum sehingga NGAL dapat dijadikan penanda diagnosis GgGA. Penelitian bertujuan mengetahui validitas NGAL urine sebagai penanda diagnosis GgGA pada penderita sepsis. Sebanyak 50 sampel urine diambil dari penderita sepsis di Unit Gawat Darurat (UGD), Intensive Care Unit (ICU), dan Medical Intermediate Care (MIC) di Rumah Sakit Dr. Hasan Sadikin Bandung selama Februari sampai Mei 2010 dan dilakukan pemeriksaan kadar NGAL urine dengan metode enzyme linked immunosorbent assay (ELISA). Data yang diperoleh dianalisis dengan uji nonparametrik Mann-Whitney, kurva receiver operating characteristic (ROC), dan uji validitas. Hasil penelitian didapatkan kadar NGAL urine penderita sepsis dengan GgGA lebih tinggi secara bermakna dibandingkan dengan penderita sepsis tanpa GgGA (3.380 ng/mL berbanding 116 ng/mL; p<0,001). Pada cut-off point 107 ng/mL, NGAL urine memiliki sensitivitas 100%, spesifisitas 36%, positive predictive value (PPV) 60,9%, negative predictive value (NPV) 100%, dan akurasi 68%. Simpulan, kadar NGAL urine memiliki validitas yang baik dan dapat dijadikan sebagai penanda diagnosis terjadinya GgGA pada penderita sepsis. [MKB. 2012;44(2):121–6]. Validity Test of Neutrophil Gelatinase Associated Lipocalin as Diagnostic Marker forAcute Kidney Injury on SepsisAcute kidney injury (AKI) is an abrupt decrease of renal function which marked by increase of serum creatinine ≥0.3 mg/dL or ≥1.5 times of previous level or decrease urine output <0.5 mL/hour in >6 hours. Sepsis is the most common cause of AKI with incidence rate is about 20–50% and mortality nearly 70%. Urine neutrophil gelatinaseassociated lipocalin (NGAL) level in AKI patients can increase quickly and earlier compared with serum creatinine and could be as a marker for AKI. The purpose of this study was to assess the validity of urine NGAL as diagnostic marker of AKI on sepsis patients. Subjects were 50 urine samples of sepsis patients from Emergency Department (ED), Intensive Care Unit (ICU) and Medical Intermediate Care (MIC) in Dr. Hasan Sadikin Hospital Bandung between February and May 2010 and were examined with enzyme linked immunosorbent assay (ELISA) method. Data analysis was performed by non parametric Mann-Whitney test, receiver operating characteristic (ROC) analysis and validity test.The results found that urine NGAL of AKI patients were significantly higher compared with non AKI patients (3,380 ng/mL vs 116 ng/mL, p<0.001). A cut-off point >107 ng/mL for urine NGAL had a sensitivity of 100%, specificity of 36%, positive predictive value (PPV) of 60.9%, negative predictive value (NPV) of 100% and accuracy of 68%. In conclusions, urinary NGAL level has good validity and could be used as a screening test for AKI on sepsis patients. [MKB. 2012;44(2):121–6]. DOI: http://dx.doi.org/10.15395/mkb.v44n2.131
Perbedaan Parameter Hematologi pada Penderita Tuberkulosis Paru Terinfeksi Mycobacterium Tuberculosis Galur Beijing dengan Galur Non-Beijing Sundari, Rini; Parwati, Ida; Mose, Johanes Cornelius; Setiabudiawan, Budi
Majalah Kedokteran Bandung Vol 49, No 1 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.157 KB) | DOI: 10.15395/mkb.v49n1.985

Abstract

Tuberkulosis (TB) merupakan penyakit inflamasi kronik, tingginya kasus TB dapat disebabkan oleh perbedaan virulensi antargalur Mycobacterium tuberculosis (MTB). Penelitian ini bertujuan untuk menganalisis berbagai manifestasi hematologi yang terjadi pada penderita TB paru yang terinfeksi  galur Beijing dan non-Beijing MTB. Sampling penelitian dilakukan di Rumah Sakit Dr. H.A. Rotinsulu Bandung, RSU Cibabat Cimahi, Balai Besar Kesehatan Paru Masyarakat (BBKPM) Bandung, Puskesmas Batujajar, Puskesmas Padalarang, dan Puskesmas Cimareme pada Juni 2014–Januari 2015. Penelitian diikuti oleh 74 penderita TB paru BTA (+) terdiri atas 61% pria dan 39% wanita yang berusia 18–63 tahun. Berdasar atas spoligotyping diperoleh 24 (32%) terinfeksi galur Beijing dan 50 (68%) galur non-Beijing. Pemeriksaan laju endap darah (LED) menggunakan metode Westergreen, parameter hematologi lain menggunakan haematology analyzer. Kadar hemoglobin galur Beijing 8,6–14,8 g/dL dan galur non-Beijing 8,1–16,5 g/dL, anemia ini lebih banyak ditemukan pada penderita yang terinfeksi galur Beijing (17 dari 24) dibanding dengan galur non-Beijing 31 dari 50. Nilai absolut eritrosit tidak ada perbedaan, kecuali red blood cell distribution width (RDW). Hasil antara Beijing dan non-Beijing didapatkan hasil LED 94,0 (35,03) vs 89,9 (29,96) mm; leukositosis tidak berbeda namun 67% neutrofilia dan 17% limfopenia pada galur Beijing, 0% dan 30% pada galur non-Beijing; jumlah trombosit 46% (416,3+161,7)x1.000 sel/mm3 vs 122-834 (407,0+154,8)x1.000 sel/mm3 dengan trombositosis 63% vs 46%. Penderita terinfeksi galur Beijing menunjukkan anemia, LED, dan trombositosis lebih tinggi dibanding dengan non-Beijing; hal ini berarti penderita terinfeksi galur Beijing mengalami inflamasi yang lebih berat. [MKB. 2016;49(1):35–41]Kata kunci: Beijing, non-Beijing, profil hematologiThe Differences of Haematology Profile in Patients with Lung Tuberculosis Infected by Mycobacterium tuberculosis Beijing Strain and non-Beijing StrainTuberculosis (TB) is a chronic inflammation disease; a high numbers of tuberculosis cases can be caused by virulence potential of each Mycobacterium tuberculosis (MTB) strain. The event of inflammation process influences the hematopoietic system which gives various hematology examination results. This study was conducted in order to analyze various forms of hematological manifestation occur in patients with lung TB caused by MTB Beijing strain and non-Beijing strain infections.  This study was performed on 74 lung TB-infected patients with positive acid-fast bacilli, consisting of 61% males dan 39% females whose age ranged from 18 to 63 (32.6+12.2) years old. Spoligotyping was performed, resulting in 24 (32%) Beijing strain and 50 (68%) non-Beijing strain infections. Hematological examination was performed using hematology analyzer and erythrocyte sedimentation rate (ESR) with Westergreen method. Hemoglobin level ranged from 8.6 to14.8 (11.8) g/dL and 8.1-16.5 (12.0) g/dL from Beijing strain and non-Beijing strain, respectively, with more anemia was found in Beijing strain patients (71%) compared to non-Beijing strain (62%). There was no differences in absolute erythrocyte count, except in red blood cell distribution width (RDW).  The comparison of ESR result between Beijing and non-Beijing in ESR resulting in 94.0 (35.03) vs 89.9 (29.96) mm with no difference in leukocytosis, yet 66.7% neutrophilia and 16.7% lymphopoiesis in Beijing strain patients, 0% and 30% consecutively in non-Beijing strain. The number of thrombocyte is 68-882 (416.3+161.7)x1000 cells/mm3 vs 122–834 (407.0+154.8)x1000 cells/mm3 with thrombocytosis in 63% vs 46%. Beijing strain patients shows anemia, and higher ESR and thrombocytosis. These show that patients infected by Beijing strains experience more severe inflammation. [MKB. 2016;49(1):35–41]Key words: Beijing strain, non-Beijing strain, haematology profile
Validitas Metode Polymerase Chain Reaction GeneXpert MTB/RIF pada Bahan Pemeriksaan Sputum untuk Mendiagnosis Multidrug Resistant Tuberculosis Sirait, Nurlina; Parwati, Ida; Dewi, Nina Susana; Suraya, Nida
Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Pengendalian tuberkulosis saat ini terkendala oleh metode diagnostik konvensional yang lambat terutama untuk mendeteksi multidrug resistant tuberculosis (MDR-TB). Deteksi dini MDR-TB sangat penting untuk mencegah penyebaran MDR-TB dan mengurangi angka kematian. Penelitian ini bertujuan menganalisis validitas pemeriksaan polymerase chain reaction genexpert MTB/RIF suatu pemeriksaan molekuler automatis yang cepat untuk mendeteksi MDR-TB. Penelitian dilakukan di Poliklinik Directly Observed Treatment Short- Course (DOTS) Rumah Sakit Dr. Hasan Sadikin Bandung. Subjek penelitian adalah penderita tersangka MDRTB. Sampel penelitian berupa dahak yang dilakukan pemeriksaan mikroskopis, uji kepekaan metode proporsi media Lowenstein Jensen dan polymerase chain reaction genexpert MTB/RIF. Selama periode Agustus 2012 sampai Januari 2013 didapatkan 88 subjek penelitian, kelompok usia terbanyak 25–34 tahun. Hasil pemeriksaan kultur didapatkan 54 sampel tumbuh, 34 sampel tidak tumbuh, dan 3 sampel merupakan Mycobacteria lain dari tuberkulosis. Sebesar 97,5% sampel yang resisten rifampisin juga resisten terhadap isoniazid. Pemeriksaan GeneXpert MTB/RIF menunjukkan sensitivitas 92,3% dan spesifisitas 75% dengan akurasi 88,2%. Simpulan, pemeriksaan GeneXpert MTB/RIF memiliki validitas tinggi untuk mendiagnosis MDR-TB terhadap baku emas uji kepekaan M. tuberculosis metode proporsi pada media Lowenstein Jensen. Pemeriksaan ini disarankan sebagai alat skrining MDR-TB. [MKB. 2013;45(4):234–9]Kata kunci: GeneXpert MTB/RIF, MDR-TB, uji kepekaan metode proporsiValidity of Polymerase Chain Reaction GeneXpert MTB/RIF Method on Sputum Sample Examination to Diagnose Multidrug Resistant TuberculosisControl of tuberculosis is hampered by slow conventional diagnostic methods especially for the detection of multidrug resistant tuberculosis (MDR-TB). Early detection of MDR-TB is essential to interrupt MDR-TB transmission and reduce the death rate. The aim of this study was to assess the validity of polymerase chain reaction genexpert MTB/RIF examination, which is a rapid automated molecular examination for the detection of MDR-TB. The study was conducted at the Directly Observed Treatment Short-Course (DOTS) clinic at Dr. Hasan Sadikin General Hospital Bandung. Subjects were patients with suspected MDR-TB. The research sample was sputum which was subjected to microscopic examination, susceptibility test proportion methods in Lowenstein Jensen media and polymerase chain reaction genexpert MTB/RIF examination. During August 2012 until January 2013, 88 subjects were obtained, with most of them were 25–34 years old. There were 54 samples obtained that grew in culture and 34 samples did not grow while 3 samples were other Mycobacteria of tuberculosis. It was also found that 97.5% of rifampin-resistant samples were also resistant to isoniazid. Examination of GeneXpert MTB/RIF showed a sensitivity of 92.3%, specificity of 75% with an accuracy of 88.2%. In conclusion, GeneXpert MTB/RIF examination has high validity for diagnosing MDR-TB against M. tuberculosis gold standard resistance test using the proportion method in Lowenstein Jensen media. The examination is recommended for MDR-TB screening. [MKB. 2013;45(4):234–9]Key words: GeneXpert MTB/RIF,MDR-TB, susceptibility test proportion methods DOI: http://dx.doi.org/10.15395/mkb.v45n4.170
Clinical and Cerebrospinal Fluid Abnormalities as Diagnostic Tools of Tuberculous Meningitis Parwati, Ida; Dian, Sofiati; Lestari, Fiona
Althea Medical Journal Vol 3, No 1 (2016)
Publisher : Althea Medical Journal

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Abstract

Background: Tuberculous meningitis (TBM) is the most severe form of extrapulmonary tuberculous (TB) disease and remains difficult to diagnose. The aim of the study was to determine the diagnostic value of clinical and laboratory findings of cerebrospinal fluid (CSF) examinations for diagnosing TBM using bacterial culture result as the gold standard.Methods: A prospective cross sectional study was carried out to 121 medical records of hospitalized TBM patients in neurological ward at Dr. Hasan Sadikin General Hospital Bandung, from 1 January 2009–31 May 2013. The inclusion criteria were medical records consisted of clinical manisfestations and laboratory findings. The clinical manisfestations were headache and nuchal rigidity, whereas the laboratory findings were CSF chemical analysis (protein, glucose, and cells) and CSF microbiological culture. Validity such as sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) for clinical and laboratory findings were calculated, using bacterial culture result as the gold standard.Results: The most clinical findings of TBM was nuchal rigidity and it had the highest sensitivity value, but the lowest spesificity value. Decreased of CSF glucose had the highest sensitivity value compared to other laboratory findings, but the value was low.Conclusions: The clinical manisfestations and the laboratory findings are not sensitive and specific enough for diagnosing TBM. [AMJ.2016;3(1):132–6] DOI: 10.15850/amj.v3n1.725
Validitas Multiplex Real Time Polymerase Chain Reaction untuk Diagnosis Limfadenitis Tuberkulosis pada Spesimen Blok Parafin Rezeki, Mike; Parwati, Ida; Hernowo, Bethy S.; Tjandrawati, Anna
Majalah Kedokteran Bandung Vol 46, No 3 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Limfadenitis tuberkulosis merupakan tuberkulosis ekstraparu yang paling sering ditemukan. Saat ini baku emas diagnosis limfadenitis tuberkulosis berdasarkan histopatologi. Pemeriksaan histopatologi tidak memberikan informasi etiologi pasti penyebab limfadenitis, padahal limfadenitis dapat disebabkan Mycobacterium tuberculosis maupun Mycobacterium non-tuberculosis yang sangat berbeda regimen terapinya. Spesimen limfadenitis tuberkulosis dalam blok parafin yang disimpan sangat bermanfaat ketika dibutuhkan pemeriksaan lanjutan.  Pemeriksaan multiplex real time polymerase chain reaction  pada  blok parafin dapat mendeteksi Mycobacterium  tuberculosis atau Mycobacterium  non-tuberculosis. Pemilihan objek penelitian dilakukan berdasarkan randomisasi sederhana. Penelitian uji diagnostik dengan  rancangan potong lintang untuk mengetahui  validitas pemeriksaan multiplex  real time polymerase chain reaction  dalam  mendiagnosis limfadenitis tuberkulosis  sebagai konfirmasi pada blok parafin dilakukan periode Juni 2012˗˗Juni 2013 di Rumah Sakit Dr. Hasan Sadikin Bandung. Objek penelitian terdiri atas 40 blok parafin limfadenitis tuberkulosis dan 22 blok parafin kelompok kontrol. Pemeriksaan multiplex real time poymerase chain reaction menggunakan primer gen sikuens sisipan IS6110 dan gen Mycobacterium protein tuberkulosis MPB64 untuk mendeteksi deoxyribonucleic acid Mycobacterium tuberculosis dan gen 16S rRNA (ribosomal ribonucleic acid) untuk mendeteksi Mycobacterium non-tuberculosis. Hasil uji validitas sensitivitas 75%, spesifisitas 77%, nilai duga positif 86,6%, nilai duga negatif 63%, dan akurasi 75,8%. Pemeriksaan multiplex real time polymerase chain reaction dapat dianjurkan untuk konfirmasi diagnosis limfadenitis tuberkulosis pada blok parafin. Simpulan: multiplex real time polymerase chain reaction  memiliki validitas sedang untuk diagnosis limfadenitis tuberkulosis pada spesimen blok parafin.Kata kunci: Deoxyribonucleic acid, limfadenitis tuberkulosis, multiplex real time polymerase chain reaction, Mycobacterium tuberculosis Validity of Multiplex Real Time Polymerase Chain Reaction for Tuberculous Lymphadenitis Diagnosis on Formalin Fixed Paraffin EmbeddedTuberculous lymphadenitis is one of the most common form of extra pulmonary tuberculous. The diagnosis of tuberculous lymphadenitis based  on  histopathology as a gold standard. Histopathology examination could not inform the etiology of lymphadenitis whether Mycobacterium tuberculosis or Mycobacterium nontuberculosis  of which  the treatment regiment is very different. The archieved specimen like formalin fixed paraffin embedded  of tuberculous lymphadenitis is useful for futher investigation. The multiplex real time polymerase chain reaction  can detect  Mycobacterium tuberculosis or Mycobacterium nontuberculosis  from formalin fixed paraffin embedded. The object of the study use simple random. The study with cross sectional design  to determine validity multiplex real time polymerase chain reaction  on tuberculous lymphadenitis from formalin fixed paraffin embedded, was done June 2012˗˗June 2013 at Dr. Hasan Sadikin Hospital Bandung. Objects  consisted  of  40  formalin fixed paraffin embedded  tuberculous lymphadenitis and  22 control group. The multiplex real time polymerase chain reaction use insertion  sequence IS6110 and Mycobacterium protein tuberculosis MPB64 gen for detect deoxyribonucleic acid Mycobacterium tuberculosis and 16S rRNA (ribosomal ribonucleic acid) gen for Mycobacterium nontuberculosis. The validity test of the Mycobacterium tuberculosis multiplex real time polymerase chain reaction with histopathology as a gold standards gave a sensitivity 75%, specificity 77%, positive predictive value 86.6%, negative predictive value 63%, and accuracy 75.8%. This examination can be used for confirmation of tuberculous lymphadenitis from formalin fixed paraffin embedded. Conclussion: multiplex real time polymerase chain reaction has intermediate validity to diagnose tuberculous lymphadentis on formalin fixed paraffin embedded.Key words: Deoxyribonucleic acid,  multiplex real time polymerase chain reaction, Mycobacterium tuberculosis DOI: 10.15395/mkb.v46n3.314
Gambaran Validitas Pemeriksaan Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Metode Rapid Immunochromatography pada Bahan Pemeriksaan Sputum dan Serum Penderita Tuberkulosis Paru Subroto, Hendra; Parwati, Ida; Turbawaty, Dewi Kartika; Alisjahbana, Bachti
Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.856 KB) | DOI: 10.15395/mkb.v49n3.1120

Abstract

Penegakan diagnosis tuberkulosis (TB) paru penting dalam mengurangi morbiditas dan mortalitas. Diagnosis laboratorium TB paru berdasar atas pemeriksaan BTA dan kultur M. tuberculosis memiliki sensitivitas rendah. Terdapat pemeriksaan cocktail antigen TB rapid immunochromatography (ICT) yang mendeteksi antigen ESAT-6, CFP-10, MPT-64 yang disekresikan oleh M. tuberculosis. Tujuan penelitian menganalisis validitas pemeriksaan cocktail antigen TB metode rapid ICT sputum dan serum penderita TB paru terhadap kultur Ogawa. Penelitian dilaksanakan Juli–Oktober 2014 di RSUP Dr. Hasan Sadikin Bandung. Bentuk penelitian adalah observasional deskriptif khusus dengan rancangan penelitian potong lintang. Subjek penelitian penderita yang datang ke Poliklinik Pulmonologi atau Poliklinik DOTS, didiagnosis TB paru. Sebanyak 68 sputum dan serum dari 33 kultur sputum M. tuberculosis positif dan 35 kultur negatif dilakukan pemeriksaan cocktail antigen TB rapid ICT. Angka positivitas cocktail antigen TB rapid ICT sputum 54,4%; serum tanpa pemanasan 0%. Pada serum dilakukan pemanasan pada suhu 56oC selama 30 menit untuk menghilangkan aktivitas antibodi dan didapatkan angka positivitas sebesar 19,1%. Nilai sensitivitas dan spesifisitas pemeriksaan untuk sputum 93,9% dan 82,8%, untuk serum tanpa pemanasan 0% dan 100%, serta serum dengan pemanasan 24,2% dan 85,7%.  Validitas pemeriksaan sputum memiliki sensitivitas tinggi dan spesifisitas sedang, untuk serum memiliki sensitivitas rendah dan spesifisitas tinggi. [MKB. 2017;49(3):178–85]Kata kunci: Cocktail antigen TB rapid ICT, kultur Ogawa, mikroskopik BTA, tuberkulosis paru, serum, sputum Validity of Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Rapid Immunochromatography Method on Sputum and Serum Samples from Patient with Pulmonary TuberculosisEarly diagnosis of pulmonary tuberculosis (TB) is very important in reducing morbidity and mortality. The current diagnosis of TB includes direct staining (acid fast bacilli) or M. tuberculosis culture, but these examinations have a low sensitivity. An assay using rapid ICT cocktail antigen TB is currently available for diagnosing TB. This method can detect ESAT-6, CFP-10, and MPT-64 antigen which is secreted by M. tuberculosis. The aim of this study was to analyze the validity of cocktail antigen TB rapid ICT using sputum and serum with Ogawa culture. This was a cross-sectional descriptive observational study. Sputum and serum were collected from patients who were diagnosed as lung TB suspects in the lung and DOTS Clinic of Dr. Hasan Sadikin General Hospital Bandung during the period of July–December 2014 in . Cocktail antigen TB detection assay using two kind of samples (sputum and serum) were evaluated. A total of 68 subjects of33 subjects presented positive culture and 35 presented negative cultures. Positivity rates for sputum and serum were 54.4% and 0%, respectively. Heated sputum assay had a sensitivity of 93.9% and specificity of 82.8%, Serum assay presented a sensitivity of 0% and specificity  of100%.  Serum were modified by heating at 56oC for 30 minutes. The positivity rate of heated serum was 19.1%. The result of modified serum assay showed a sensitivity  of 24.2% and specificity of 85.7%. Conclusion: the sensitivity of the sputum assay is high and the specificity is medium. The sensitivity of this serum assay is low and the specificity is high. [MKB. 2017;49(3):178–85]Key words: Acid fast bacilli, cocktail antigen TB rapid ICT, pulmonary tuberculosis, Ogawa culture, sputum, serum
Co-Authors - Hidayat, - - Liza A K Sugianli A Rizal Abdul Hadi Martakusumah Adhi K Sugianli Adhi Kristianto Sugianli Adrian Suhendra Agnes Rengga I Agnes Rengga Indrati Ahmad Faried Ahmad Rizal Ahmad Rizal Ganiem Al Rasyid Amanda Widayanti Andani Eka Putra Andi Basuki Prima Birawa Andi Basuki Prima Birawa Andre van der Venn Anggra Filani Anggraini Alam Ani Melani Maskoen Anis Karuniawati Anna Tjandrawati Arto Yuwono Arto Yuwono Soeroto, Arto Yuwono Astrid Tamara Maajid Budiman Bachti Alisjabana, Bachti Bachti Alisjahbana Bachti Alisyahbana Basti Andriyoko Basti Andriyoko Bethy S. Hernowo Bethy Suryawati Hernowo BUDI SETIABUDIAWAN Budi Setiabudiawan Budiman , Budiman Chrysanti Murad Chrysanti Murad, Chrysanti Cissy B Kartasasmita Cissy B. Kartasasmita Coriejati Rita D Oktavia Dahlan, Zulkarnain Delita Prihatni Dewi Kartika T Dewi Kartika Turbawaty Dewi Kartika Turbawaty Dewi Lokida Dewi Nurhayati Dick van Soolingen Dida Ahmad Gurnida Djatnika Setiabudi E. Risdiyani Edhyana Sahiratmadja Efrida Efrida Efriyani, Yuhpita Indah Elsa Yulius Endang Samaun Endang Sutedja Erica Catarina Erna Jupri Evan Susandi Fairuz Quzwain Fiona Lestari Fiona Lestari, Fiona Ganiem AR Gde Sindu Mega Hartanto Bayuaji Hendra Subroto, Hendra Herri S Sastramihardja Herry Garna Hinta Meijerink I. Sjahid Idaningroem Sjahid Ike Sri Redjeki Indahwaty, - Ivana Agnes Sulianto J. E. Gunawan Jamsari Jamsari Johanes C. Mose Johanes C. Mose Julia Hartati Juniati, Rianita Larissa Larissa Lelly Yuniarti Leni Lismayanti Lidya Chaidir Linda Choerunnisa Lisda Amalia Livia Noviani Liza Liza Luhung Budiailmiawan Mike Rezeki Nanan Sekarwana Nataprawira, Heda M Nenny Gustiani Nenny Gustiani Ni Sayu Dewi B. Nida Suraya Nida Suryana Nina Susana Dewi Nina Tristina Noormartany . Nur Atiik Nurizzatun Nafsi Nurlina Sirait Prawoto Prawoto Prayudi Santoso Pujiyanti, Hapsari Rachmat Soelaeman Rahmat Sumantri Ramdan Panigoro Reinout van Crevel Reinout van Crevel RINI SUNDARI RINI SUNDARI, RINI Rita Rachmayanti Rizki Andriyani Rubin Surachno Gondodiputro Rudi Wisaksana Rully Marsis Amirullah Roesli Ryan Bayusantika S. Nilawati Silvia Rachmayati Soeroto, Arto Y Sofiati Dian Sylvia Rachmayati T. M. Sudiro Tiene Rostini Tiene Rostini Tiene Rostini Tiene Rostini, Tiene Tjahjani M. Sudiro Towifah Fauziah Choerunisa Turbawati DK Uni Gamayani Uun Sumardi Wia Melia Wida Purbaningsih Wulan Ardhana Iswari Wulan Ardhana Iswari, Wulan Ardhana Yani Triyani Yani Triyani Yanti Yanti Yeva Rosana Yovita Hartantri Yusuf Sulaiman Effendi