cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. aceh besar,
Aceh
INDONESIA
Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
Arjuna Subject : -
Articles 12 Documents
 1   2 
Search results for , issue "Vol 4, No 2 (2014): Life Sciences" : 12 Documents clear
FAECAL GLUCOCORTICOID MEASUREMENT AS INDICATOR STRESS IN WILD CRESTED MACAQUES (MACACA NIGRA): THE IMPORTANCE OF VALIDATION AND SAMPLE PROCESSING TECHNIQUES . Gholib; Taufiq P. Nugraha; Muhammad Agil; Iman Supriatna; Bambang Purwantara; Antje Engelhardt
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.231 KB)

Abstract

Monitoring of physiological stress in wild and/or endangered animals living in their natural habitat can be generated via measuring faecal glucocorticoid metabolites (FGCMs) through enzyme immunoassay technique. However, a careful validation of each enzyme immunoassays (EIAs) and methodological issues such as samples preservation, and extraction procedures must be a significant concern and validated because  it may influence FGCMs levels. In this present study, the aim was to carry out a biological validation to find reliable EIAs that could be used for non-invasive measurement of glucocorticoid levels in crested macaques. Moreover, we compared three different techniques of preservation: lyophilisation, oven drying and field extraction and three different solvents for extraction: 80% methanol, 90% pure ethanol and 90% commercial alcohol (isopropyl alcohol) to find the simple and practical techniques for sample processing. For hormone analysis, we colIected 189 faecal samples from wild crested macaques living in the Tangkoko-Batuangus Nature Reserve, North Sulawesi, Indonesia. The results show that biological validation can be used to validate measurement of faecal glucocorticoid which 3α,11β-dihydroxy-etiocholanolone was the most reliable EIAsfor this species. Different preservation and solvent extraction were not significantly influenced levels of FGCMs (p0.05). In conclusion, Our study highlights the importance of biological validation of FGCMassays and presents practical techniques for the non-invasive monitoring of physiological stress in crested macaques.
BROAD SPECTRUM ANTIMICROBIAL ACTIVITY OF LUMBRICUS RUBELLUS POWDER AGAINST DRUG RESISTANT MICROBES TRISTIA RINANDA; . SAKDIAH; RISNA OKTAVIA; MEYCHA DA FHONSA; MUFQI FITRA
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.054 KB)

Abstract

Inappropriate uses of antimicrobial drugs have contributed to the development of resistant microbial pathogens. This phenomenon requires discovery and development of potential antimicrobial compounds from organic materials. Lumbricus rubellus is the ubiquitous earthworm containing antimicrobial peptide named Lumbricin-1. The aim of this study was to determine the broad spectrum antimicrobial activity of Lumbricus rubellus powder against several drug resistant microbes such as Multidrug Resistant (MDR) Pseudomonas aeruginosa, Methicillin Resistant Staphylococcus aureus (MRSA) and Fluconazole resistant Candida albicans. This experimental study was conducted using Completely Randomized Design (CRD) with 6 treatment groups consisting of 100 mg, 200 mg, 300 mg, 400 mg and 500 mg L.rubellus powder in 5 ml solvent (acetic acid and acetonitrile) and negative control groups. One percent of each concentration then was used in the antimicrobial activity testing using Kirby Bauer disc diffusion method. Data of this research were analyzed by ANOVA. The results showed that L. rubellus powder in each of the concentrations of 100 mg, 200 mg, 300 mg, 400 mg and 500 mg obtained an average inhibition zone of 14.33 mm, 14.17 mm, 14.00 mm, 15.00 mm and 13.00 mm respectively against MDR P. aeruginosa; 16.75 mm, 18.50 mm, 18.75 mm, 16.75 mm and 17.25 mm against MRSA; 16.50 mm, 18.00 mm, 17.50 mm, 17.75 mm and 16 mm against Fluconazole resistant C. albicans. Statistical analysis revealed that Lumbricus rubellus powder in the tested concentrations showed significant broad spectrum antimicrobial activity against drug resistant microbes (p0.05).

Page 2 of 2 | Total Record : 12

 1   2 

Filter by Year

2014 2014


Reset
Filter By Issues
All Issue Vol 5, No 2 (2015): Life Sciences Vol 5, No 1 (2015): Engineering Vol 4, No 2 (2014): Life Sciences Vol 4, No 1 (2014): Engineering Vol 3, No 3 (2013): Addendum Vol 3, No 2 (2013): Engineering Vol 3, No 1 (2013): Life Sciences Vol 2, No 2 (2012): Engineering Vol 2, No 1 (2012): Life Sciences Vol 1, No 2 (2011): Engineering Vol 1, No 1 (2011): Life Sciences More Issue