Claim Missing Document
Check
Articles

Found 40 Documents
Search

MP-12 Effect of AKBISprob Supplementation on Antibacterial-Producing Lactic Acid Bacteria (LAB) Isolated from Laying Hens Intestine . Nurliana; Sema Coumandary; Fashihah Rahmah Noya Farimansysh; . Sugito; Dian Masyitha; . Darmawi; . Erina; . Gholib; Erdiansya Rahmi; . Ismail
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.168 KB)

Abstract

Generally, Bacteria in the intestine consist of beneficial bacteria and bacteria that have the potential to disrupt animal health such as pathogenic bacteria. The presence of pathogenic bacteria is one of the factors that cause gastrointestinal dysfunction, but can be suppressed by maintaining the presence of beneficial bacteria for the digestive tract, especially the type of Lactic Acid Bacteria (LAB) (Astuti, 2016). LAB has antagonistic activity because it is able to inhibit pathogenic bacteria so it can compete to maintain normal flora balance in the digestive tract.  The main inhibitory activity of LAB is caused by the accumulation of primary metabolites such as lactic acid, acetic acid, ethanol, and carbon dioxide. In addition, LAB is also capable of producing antibacterial compounds such as bacteriocins (Furtado et al., 2014). Naturally LAB has existed in the digestive tract of chickens, but it is easy to experience changes in the amount due to the influence of given the feed (Widodo et al., 2015). Feed and the environment can affect the composition of microbes in the digestive tract in chickens(Ghadban, 2002; Apajalahti et al., 2004). Provision of fermented feed can improve the composition of intestinal microflora and increase the number of LAB. Fermented feed is generally easily biodegradable and has a higher nutritional value than the original ingredient and it can reduce the pH of the digestive tract.AKBISprob is an alternative product to increase poultry production in the form of supplement made from a mixture of soybean wate, and palm kernel meal which is fermented with Aspergillus niger.  The fermentation process using Aspergillus niger can reduce the crude fiber contained in AKBIS, because the mold can produce amylase, pectinase, amyloglucosidase and cellulase enzymes which can degrade cellulose so that it is easier to digest. In addition, Aspergillus niger also produces metabolites in the form of citric acid which is a component that can reduce intestinal pH so that it is suitable for LAB growth. Based on research conducted by Nurliana et al. (2016; 2017), supplementation of 4% AKBISprob can maintain the chickens production and health as well increase the number of LAB and reduce the number of pathogenic bacteria such as Escherichia coli and Salmonella sp. in chickens intestine. Thus creating a balance of microflora in the digestive tract of laying hens. Based on the above reasons, it is necessary to conduct research on antibacterial detection of BAL in laying hens that have been given AKBISprob.
PCS-9 Effect of Freeze Drying of Follicular Fluid from Waste of Aceh Cattle Ovary on the Estradiol Concentration M Adam; G Gholib; M Hamdan; M Syafruddin; Hafizuddin Hafizuddin; H Alief
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.81 KB)

Abstract

Aceh cattle are one of the many cow’s species that are widely preserved and distributed in Aceh Province. This species is the result of a cross between Bos sundaicus and Bos indicus and has been designated as a native Indonesian class of cattle in 2011 by the Minister of Agriculture of Indonesia through the decision of the Minister of Agriculture number 2907 / Kpts / OT.140 / 6/2011 [1]. Aceh's cattle population in 2017 in total was 627,629 species [2]. Slaughtering the cows results in the obtaining of byproduct or waste such as ovaries. The ovarium of female cows is one of the complement results of animal slaughter house which is infrequently used. In fact, the ovaries comprise follicles which contain many active compounds inside the follicular fluids such as insulin-like growth factor I (IGF-I), follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen and progesterone [3]. Freeze drying/lyophilization is one of the preservative process which are generally used in food and non-food processing. This study was conducted to determine the effect of these methods on the concentration of estrogen in follicular fluid.
AH-25 Sperm Morphology of the Javan Muntjak, Muntiacus muntjak muntjak Sri Wahyuni; Gholib Gholib; Wahono Esthi Prasetyaningtyas; I Ketut Mudite Adnyane; Srihadi Agungpriyono; Hamny Hamny; Muhammad Jalaluddin; Mustafa Sabri; Muslim Akmal; Muhammad Agil; Tuty Laswardi Yusuf
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.056 KB)

Abstract

Sperm Morphology of the Javan Muntjak,  Muntiacus muntjak muntjak
Evaluasi Validitas Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit dan Waktu Sentrifugasi Sampel Darah untuk Pengukuran Konsentrasi Hormon Kortisol pada Kambing Kacang Gholib Gholib; Sri Wahyuni; Rahma Melinda; Muslim Akmal
Jurnal Agripet Vol 21, No 1 (2021): Volume 21, No. 1, April 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i1.18153

Abstract

ABSTRAK. Penggunaan human cortisol enzyme-linked immunosorbent assay (ELISA) kit untuk pengukuran hormon kortisol pada hewan dan keterlambatan waktu sentrifugasi sampel darah untuk analisis hormon perlu dievaluasi. Penelitian ini bertujuan untuk mengevaluasi validitas human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) untuk pengukuran konsentrasi kortisol dan menguji pengaruh keterlambatan waktu sentrifugasi sampel darah terhadap stabilitas konsentrasi kortisol pada kambing kacang. Sampel darah dikoleksi dari delapan ekor kambing kacang. Uji validitas kit EIA-1887 dilakukan secara: a) analitik (uji parallelism, akurasi, dan presisi), dan b) biologis (pengukuran kortisol sebelum dan setelah transportasi). Uji keterlambatan waktu sentrifugasi terhadap stabilitas konsentrasi kortisol dilakukan dengan 5 perlakuan yaitu disentrifugasi kurang dari 1 jam (P1/kontrol), 6 jam (P6), 12 jam (P12), 18 jam (P18),dan 24 jam (P24) setelah darah dikoleksi. Data uji parallelism dianalisis dengan uji persamaan kemiringan, uji presisi dihitung % CV (coefficient variation) intra-assay dan inter-assay, uji akurasi dihitung % recovery, uji T untuk validasi biologis, dan uji ragam (One Way Anova) untuk pengaruh waktu sentrifugasi. Hasil uji parallelism menunjukkan kurva sampel kambing kacang sejajar/parallel dengan kurva standar kortisol. Akurasi kit EIA-1887 adalah 103,43±7,85%, dan % CV intra-assay dan inter-assay adalah 10%. Konsentrasi kortisol setelah transportasi secara signifikan lebih tinggi daripada sebelum transportasi (p0,05). Adanya penurunan secara nyata konsentrasi kortisol pada darah yang disentrifugasi 24 jam (P24) setelah koleksi (p0,05). Kesimpulan, human cortisol ELISA kit (EIA-1887) memiliki validitas yang baik secara analitik dan biologis untuk digunakan dalam pengukuran konsentrasi kortisol pada kambing kacang. Keterlambatan waktu sentrifugasi selama 24 jam berpengaruh terhadap konsentrasi kortisol.  (Evaluation the Validity of Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit and Centrifugation Time of Blood Sample for Measuring the Concentration of Cortisol in Kacang Goats)  ABSTRACT. The use of human cortisol ELISA kit for measuring cortisol in animals and delayed to blood centrifugation time for hormone measurement need to be evaluated. This study aimed to evaluate the validity of human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) for cortisol measurement and effect of delayed to blood centrifugation time on cortisol concentrations in kacang goats. Blood was collected from eight kacang goats. Validation test of EIA-1887 kit was performed through: a) analytical (parallelism, accuracy, and precision tests), and b) biological validations (measuring cortisol concentrations before and after transportation). Five treatments were performed to test delayed to centrifugation time: blood centrifuged at 1 h (control, P1), 6 h (P6), 12 h (P12), 18 h (P18), and 24 h (P24) after collection. Parallelism data were analyzed by slope equality test, precision and accuracy calculated by % CV of intra-and inter-assay, and % recovery, respectively. Data of biological validation and centrifugation time effects were analyzed by Student t-test, and one way ANOVA, respectively. Results of parallelism showed that serial dilution curve of kacang goat plasma was parallel with cortisol standard curves. Accuracy of EIA-1887 kit was 103.43±7.85%, and % CV of intra-and inter-assay were 10%. Concentration of cortisol after transportation was significantly higher than before transportation (p0.05). Concentration of cortisol was significantly decreased when blood was centrifuged at 24 h after collection (P0.05). In conclusion, human cortisol ELISA kit (EIA-1887) is a reliable assay for measuring cortisol in plasma of kacang goat. Delayed to blood centrifugation time affect cortisol concentrations.
Pengaruh Pemberian PGF2α Sebelum Koleksi terhadap Peningkatan Kualitas Semen dan Level Testosteron Sapi Aceh Eka Meutia Sari; Saifan Nur; Mulkan Mulkan; Gholib Gholib; Cut Nila Thasmi; Tongku Nizwan Siregar
Jurnal Agripet Vol 21, No 1 (2021): Volume 21, No. 1, April 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i1.17778

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian hormon PGF2α sebelum koleksi terhadap peningkatan kualitas semen dan level testosteron sapi Aceh di UPT. Hewan Coba Universitas Syiah Kuala. Penelitian ini dilaksanakan dari bulan Januari sampai dengan Maret 2019. Data hasil pemeriksaan kualitas semen dianalisis secara deskriptif. Dalam penelitian ini digunakan satu ekor sapi Aceh jantan sebagai kontrol dengan 2 perlakuan, yaitu P1 (tanpa pemberian PGF2α sebelum koleksi) dan P2 (pemberian PGF2α 30 menit sebelum koleksi) dengan dosis 25 mg tiap perlakuan. Setiap perlakuan dilakukan sebanyak lima kali dan dilakukan secara bergantian setiap minggunya. Rata-rata volume (ml); konsentrasi (106 sel/ml); motilitas (%), viabilitas (%); abnormalitas (%); dan level testosteron (ng/ml) pada P1 vs P2 masing-masing adalah 5,2±1,30 vs 5,2±0,97 (P0,05); 145,4±36,58 vs 172,8 ±46,27 (P0,05); 63,87±6,44 vs 75,05±0,08 (P0,05); 69,30±0,22 vs 75,05±0,08 (P0,05); 16,90±0,08 vs 10,46±0,09 (P0,05); dan 28,07±14,37 vs 24,19±6,11 (P0,05). Disimpulkan bahwa pemberian PGF2α 30 menit sebelum koleksi dapat meningkatkan kualitas spermatozoa tetapi tidak dapat meningkatkan konsentrasi testosteron.  (Case study the effect of giving pgf2α before the collection of the quality of Aceh cattle semen) ABSTRACT. This study aims to determine the effect of giving PGF2α hormone before collection to improve the quality of Acehnese semen in UPT. Hewan Coba Syiah Kuala University. This research was conducted from January to March 2019. Data on semen quality examination were analyzed descriptively. In this study, one Aceh cattle was used as a control with 2 treatments, namely P1 (without administration of PGF2α before collection) and P2 (administration of PGF2α 30 minutes before collection) at a dose of 25 mg per treatment. Each treatment was carried out five times and carried out alternately every week. The results showed that the average volume of cement (ml) and pH P1 and P2 respectively 5.2 ± 1.30 and 5.2 ± 0.97, and 6.6±0.55 and 6.5 ± 0.50. While the average concentration of spermatozoa (106 cells / ml) in P1 and P2 were 145.4 ± 36.58 and 172.8 ± 46.27 respectively. The average percentage (%) of progressive spermatozoa motility, circularity, non motility, viability, and abnormalities in P1 and P2 respectively: 63.87 ± 6.44 and 73.63 ± 9.42, 11.34 ± 3, 70 and 9.0 ± 7.01, 24.79 ± 4.04 and 17.37 ± 4.44, 69.30 ± 0.22 and 75.05 ± 0.08, 16.90 ± 0.08 and 10.46 ± 0.09. It was concluded that administration of PGF2α 30 minutes before collection can increase the quality of spermatozoa but cannot increase the concentration of testosterone.
Efektivitas Pemberian Beberapa Preparat Hormon Prostaglandin Komersial terhadap Persentase Berahi Sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara Novika Ayuni Rambe; Tongku Nizwan Siregar; Teuku Armansyah TR; Gholib Gholib; Budianto Panjaitan; Mulyadi Adam; Dasrul Dasrul
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16317

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh perbedaan PGF2α analog terhadap persentase berahi sapi di Labuhanbatu Selatan. Sampel yang digunakan dalam penelitian ini adalah data hasil UPSUS SIWAB tahun 2017. Dalam penelitian ini digunakan data 2.547 ekor sapi yang memiliki variasi breed dan umur, dengan skor kondisi tubuh baik. Sapi-sapi dikelompokkan menjadi tiga kelompok berdasarkan preparat sinkronisasi berahi yang digunakan. K1= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%) berjumlah 1.300 ekor. K2= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-2 (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml) berjumlah 600 ekor. K3= kelompok sapi yang diinjeksi dengan 2 ml PGF2α-3 (cloprostenol 75 mg/ml dan chlorocresol 1,0 mg/ml) berjumlah 647 ekor. Penyuntikan dilakukan secara intramuskulus, dua kali dengan interval 10 hari. Data yang diperoleh kemudian dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Withney. Hasil analisis menunjukkan persentase berahi pada kelompok 1; 2; dan 3 masing-masing 80,7%; 50% dan 61,8% (P0,01). Dari hasil penelitian dapat disimpulkan bahwa PGF2α-1 mempunyai efektivitas lebih baik dibandingkan PGF2α-2 dan PGF2α-3 dalam induksi sinkronisasi berahi pada sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara.  (Effectiveness of different commercial prostaglandin hormone administration on the percentage of estrous in cattles in South Labuhanbatu, North Sumatera) ABSTRACT. The aim of this study was to determine the effect of differences PGF2α analogues on the percentage of estrus in cattles in South Labuhanbatu. The sample used in this study was the 2017 SIWAB UPSUS data. Data of 2,547 cattles with various breed and age as well as in good body condition scores were selected. The cattles were grouped into three data groups based on the hormone used for estrus synchronization. The cattles in data group I (1300 cows) were injected with 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml and benzyl alcohol 1.65%). The cattles in data group II (600 cows) were injected with 5 ml PGF2α-2 (5.5 mg/ml dinoprost tromethamine and 12.0 mg/ml benzyl alcohol). The cattles in data group III (647) were injected with 2 ml of PGF2α-3 (cloprostenol 75 µg/ml and chlorocresol 1.0 mg/ml). Injections were carried out intramuscularly, twice at 10-day intervals. The data obtained were then analyzed Kruskal Wallis and continued with Mann Withney test. The results showed that the percentage of estrus in groups I; II; and III were 80.7%; 50% and 61.8% (P0.01). From the results of the study it can be concluded that Lutalyse has better effectiveness than Capriglandin and Sincrovall in the induction of synchronization estrus in cattle in South Labuhanbatu, North Sumatera.
FAECAL GLUCOCORTICOID MEASUREMENT AS INDICATOR STRESS IN WILD CRESTED MACAQUES (MACACA NIGRA): THE IMPORTANCE OF VALIDATION AND SAMPLE PROCESSING TECHNIQUES . Gholib; Taufiq P. Nugraha; Muhammad Agil; Iman Supriatna; Bambang Purwantara; Antje Engelhardt
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.231 KB)

Abstract

Monitoring of physiological stress in wild and/or endangered animals living in their natural habitat can be generated via measuring faecal glucocorticoid metabolites (FGCMs) through enzyme immunoassay technique. However, a careful validation of each enzyme immunoassays (EIAs) and methodological issues such as samples preservation, and extraction procedures must be a significant concern and validated because  it may influence FGCMs levels. In this present study, the aim was to carry out a biological validation to find reliable EIAs that could be used for non-invasive measurement of glucocorticoid levels in crested macaques. Moreover, we compared three different techniques of preservation: lyophilisation, oven drying and field extraction and three different solvents for extraction: 80% methanol, 90% pure ethanol and 90% commercial alcohol (isopropyl alcohol) to find the simple and practical techniques for sample processing. For hormone analysis, we colIected 189 faecal samples from wild crested macaques living in the Tangkoko-Batuangus Nature Reserve, North Sulawesi, Indonesia. The results show that biological validation can be used to validate measurement of faecal glucocorticoid which 3α,11β-dihydroxy-etiocholanolone was the most reliable EIAsfor this species. Different preservation and solvent extraction were not significantly influenced levels of FGCMs (p0.05). In conclusion, Our study highlights the importance of biological validation of FGCMassays and presents practical techniques for the non-invasive monitoring of physiological stress in crested macaques.
3. The Effect of Audio Recording for Playback Experiment to the Alteration of Orangutan’s Territorial Behavior Rahmat Nazif; Gholib Gholib; Erdiansyah Rahmi; Arman Sayuti; Triva Murtina Lubis; Ummu Balqis
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.89 KB) | DOI: 10.21157/j.med.vet..v12i1.4253

Abstract

The aim of this study was to find an alternative method to prevent the conflict between the orangutan and human with the utilization of audio recording as biofence. The location of this study was in Leuser National Park on Bukit Lawang, Langkat, North Sumatra using 16 orangutans as the subject of this study. The method used in this study was a simulation and playback experiment which were using long call, spontaneous call, predator voice, and siren sound in the audio recording of non-alpha orangutans. The respond was observed using binocular and recorded using camera recorder. The data obtained were analyzed using Krustal Wallist test. The result showed that 87% of orangutan showed an interest in the attractor, on the other hand, 13% showed disinterest to the attractor. The facial expression shown by orangutan was fear, submission, aggression, and worry. The statistical analyzing result showed that long call and the spontaneous call was very significant (P0.01), while the predator and siren voice were insignificant (P0.05). The result can be concluded that long call and spontaneous calls showing the effective result to the alteration of territorial behavior on orangutan and have a potential as a biofence that is used as an alternative method to prevent the conflict between orangutan and human.
7. Total Level of Hemoglobin and Hematocrit Value of Broiler Chickens after Given Moringa Leaves Fermented Flour (Moringa oleifera) in Feed M. Hasan; Octa Sicillia Rampai; Arman Sayuti; Razali Daud; Abdul Harris; T. Armansyah TR; Gholib Gholib
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.4299

Abstract

This study aimed to determine the effect of using Moringa leaves fermented flour in feed stock toward the increasing level of hemoglobin and hematocrit value on broiler chickens. This study used a completely randomized design that consists of 4 treatments and 5 replications. The study group was divided into group P0 which was given with 100% of commercial feed, group P1 was given 95% of commercial feed and Moringa leaves fermented flour 5%, group P2 was given 92.5% of commercial feed and Moringa leaves fermented flour 7.5%, and group P3 was given 90% of commercial feed and Moringa leaves fermented flour 10%. Fermentation using EM4 (Effective Microorganism-4) were able to decrease the crude fiber of Moringa leaves flour. The treatment was conducted for 15 days, then hemoglobin levels and hematocrit values were counted. Data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. This research showed that giving Moringa leaves fermented flour for 15 days did not have a significally effect (P0.05). The given Moringa leaves fermented flour in  feed for 15 days with 5%, 7.5% and 10% concentration was not show any increase in the levels of hemoglobin and hematocrit values.
16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract Rizki Aulia; Sugito Sugito; M. Hasan; T. Fadrial Karmil; Gholib Gholib; Rinidar Rinidar
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.49 KB) | DOI: 10.21157/j.med.vet..v11i2.4667

Abstract

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella
Co-Authors . Darmawi . Erina . Ismail . Nurliana Abdul Harris Abdullah Hamzah Afifuddin Afifuddin Al Azhar Amiruddin A Amiruddin Amiruddin Andre Pasetha Antje Engelhardt Antje Engelhardt Arman Sayuti Arman Sayuti Azhar A Bambang Purwantara Budianto Panjaitan Budianto Panjaitan Chintya Desfariza Cut Dahlia Iskandar Cut Erika Ramadhana Cut Nila Thasmi Cut Nila Thasmi Dasrul Dasrul Dasrul Dasrul Dian Masyitha Dwinna Aliza DYAH PERWITASARI -FARAJALLAH Eka Meutia Sari Erdiansya Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Fadli A. Gani Fadli A. Gani Farida Athaillah Fashihah Rahmah Noya Farimansysh Fisma Amri H Alief Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Hamny Sofyan Hasanuddin Hasanuddin Herrialfian Herrialfian Husnurrizal . Ika Inda Bani Iman Supriatna Joharsyah J Juli Melia Juli Melia Ketut Adnyane Mudite Lilis Suryani M Adam M Hamdan M Hasan M Syafruddin M. Hasan M. Hasan M. Isa Mahdi Abrar Mahdi Abrar Michael Heistermann Muhammad Adlim MUHAMMAD AGIL Muhammad Bahi Muhammad Hambal Muhammad Hambal Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Jalaluddin Mulkan Mulkan Mulyadi Adam Mulyadi Adam Mulyadi Adam Mulyadi Adam Muslim Akmal Muslim Akmal Muslim Akmal Nabilah Putroe Agung Nanda Yulian Syah Nellita Mutia Novika Ayuni Rambe Nuzul Asmilia Octa Sicillia Rampai Okta Hilda Kadar Patrick Flaggellata Rahma Melinda Rahmat Nazif Rasmaidar Rasmaidar Razali Daud Razali Daud Rinidar R Rinidar Rinidar Riski Ananda Rizki Aulia Rosa Rika Wahyuni Roslizawaty R Roslizawaty Roslizawaty Rumi Sahara Zamzami Saifan Nur Sema Coumandary Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Suriadi S Syafruddin S Syafruddin Syafruddin Syifa Salsabila T. Armansyah T. Armansyah T. Armansyah TR T. Armansyah, TR T. Fadrial Karmil Taufiq P. Nugraha Taufiq Purna Nugraha Teuku Armansyah Teuku Armansyah Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Triva Murtina Lubis Triva Murtina Lubis Tuty Laswardi Yusuf Ummu Balqis Usma Aulia Utari Desya Wahono Esthi Prasetyaningtyas Wahyuni, Sri Yana Zein Yezi Gita Rahayu Zainuddin Z ZK Abdurahman Baizal Zuhrawati Zuhrawati Zulkarnain Zulkarnain