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AL KAUNIYAH
ISSN : 19783736     EISSN : 25026720     DOI : 10.15408/kauniyah
Core Subject : Science,
Al-Kauniyah: Jurnal Biologi (p-ISSN: 1978-3736, e-ISSN: 2502-6720) is an Open Access Journal published by Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, and established since 2007. Since 2016 Al-Kauniyah has established a collaboration with the Association of Lecturer in Biology and Biology Education throughout the State Islamic Higher University (PTKIN) in Indonesia. Until 2015, Al-Kauniyah covered environmental biology solely, but since 2016 the journal has been extended to cover the entire field of biological science (bioscience). By publishing biannually, on April and October, Al-Kauniyah is intended to communicate original researches and current issues on the subject of biology. Since volume 9 issue 1 April 2016, Al-Kauniyah had been changes the layout. This journal warmly welcomes contributions from scholars of related disciplines. Manuscripts can be submitted to AL-KAUNIYAH
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COVER AL-KAUNIYAH JURNAL BIOLOGI VOL. 11 NO. 1 APRIL 2018 COVER COVER
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.994 KB) | DOI: 10.15408/kauniyah.v11i1.9990

Abstract

STUDI PAKAN KUKANG JAWA (Nycticebus javanicus Goeffroy, 1812) DI TALUN DESA CIPAGANTI, GARUT, JAWA BARAT Helmi Romdhoni; Ratna Komala; Marie Sigaud; K. A.I. Nekaris; Agung Sedayu
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (706.668 KB) | DOI: 10.15408/kauniyah.v11i1.4914

Abstract

Abstrak Talun atau hutan kebun memiliki potensi sebagai habitat kukang jawa (Nycticebus javanicus) di luar kawasan yang dilindungi. Salah satu karakter habitat adalah keberadaan pakan dalam habitat. Penelitian ini dilakukan pada bulan Juli hingga September 2016, bertempat di kawasan talun Desa Cipaganti, Garut, Jawa Barat. Metode yang digunakan adalah metode deskriptif. Sampel penelitian adalah kukang dewasa yang sudah dipasang radio-collar. Pengambilan data pakan berupa jenis pohon dan jenis pakan yang digunakan.Data pakan dikumpulkan selama pengamatan perilaku. Metode yang digunakan dalam pengamatan perilaku adalah instantaneous point sampling dengan interval waktu lima menit. Pengambilan data vegetasi habitat  dilakukan dengan mengamati struktur dan komposisi vegetasi. Jenis pakan yang dimakan oleh kukang jawa jantan dan betina dianalisis dengan menggunakan Chi-square. Terdapat tiga jenis pakan utama dari kukang jawa, yaitu getah (71,27%), nektar (16,09%), serangga (11,49%), dan bunga (1,15%). Terdapat tiga suku tumbuhan yang digunakan oleh kukang jawa sebagai sumber pakan, yaitu  Fabaceae, Arecaceae, dan Moraceae.  Abstract Talun or forest-garden has a potential role as habitat for javan slow loris (Nycticebus javanicus) outside its protected area. One of the habitat characteristics is the presence of feeds in the habitat. The study was conducted from July to September 2016, in a talun area of Cipaganti Village, Garut, West Java. The method used in this study was descriptive. The samples of the study were adult individuals that had been installed with a radio-collar. Data on tree species as feeds were collected during observations on behavior. The method used in the behavior observation was instantaneous point sampling with five-minute intervals. Data retrieval of the vegetation was conducted by observing the structure and composition of the vegetation. Types of the feed consumed by the male and female animals were analyzed by using Chi-square test. There were three types of main feeds for javan slow loris, those were gum (71.27%), nectar (16.09%), insect (11.49%), and flower (1.15%). There were five families of plant that used by the javan slow loris as the source of feeds.  Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 4914  
CATATAN TENTANG Solanum diphyllum L. (SOLANACEAE) TERNATURALISASI DI PULAU JAWA Muhammad Rifqi Hariri; Arifin Surya Dwipa Irsyam
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (834.869 KB) | DOI: 10.15408/kauniyah.v11i1.5448

Abstract

Abstrak Solanum merupakan salah satu marga terbesar pada kelompok tumbuhan Angiospermae. Marga tersebut terdiri dari 1400 jenis yang terdistribusi di kawasan tropis dan subtropis. Banyak anggotanya yang memiliki nilai ekonomi tinggi, sehingga telah dibudidayakan secara luas sebagai tanaman pangan, sayuran, tumbuhan obat, dan tanaman hias. Oleh sebab itu, beberapa di antaranya telah diintroduksi ke Pulau Jawa sejak lama. Sebanyak 24 jenis Solanum telah tercatat dalam buku Flora of Java vol. 2. Meskipun demikian, masih memungkinkan adanya jenis-jenis lain yang belum terekam dalam buku tersebut. Penelitian ini bertujuan untuk memberikan informasi mengenai jenis tambahan yang telah ternaturalisasi di Pulau Jawa. Pengamatan dilakukan di beberapa lokasi di Jawa Barat, Jawa Tengah, dan Jawa Timur pada tahun 2015 hingga 2017. Solanum diphyllum telah dikoleksi dari Bogor (Darmaga), Bandung (Taman Sari dan Pasir Impun), Sumedang (Jatinangor), Wonogiri (Desa Johunut), Semarang (Desa Mesu), Trenggalek (Desa Watulimo), Situbondo (Desa Wringin Anom dan Banyuputih), Bondowoso (Desa Bandilan), dan Sumenep (Desa Pakong). Jenis ini memiliki potensi untuk dimanfaatkan sebagai tumbuhan obat. Tumbuhan ini ditemukan tumbuh meliar di semua lokasi pengamatan dan keberadaannya perlu diperhatikan karena jenis tersebut merupakan tumbuhan asing invasif di kawasan lain.  Abstract Solanum is one of the largest genera in Angiospermae. This genus comprises about 1400 species distributed in tropic and subtropic regions. Some of them have high economic value and have been cultivated widely as crops, vegetables, medicinal plants, and ornamental plants. Therefore, several species have been introduced to Java island in the past. A total of 24 species of Solanum has been recorded in Flora of Java vol. 2. However, there is still unrecorded species occurred. The aim of this study was to provide information about an additional naturalized species in Java. The observations have been carried out in several locations in West Java, Central Java, and East Java, from 2015 to 2017. Solanum diphyllum has been collected from Bogor (Darmaga), Bandung (Taman Sari and Pasir Impun), Sumedang (Jatinangor), Wonogiri (Johunut Village), Semarang (Mesu Village), Trenggalek (Watulimo Village), Situbondo (Wringin Anom and Banyuputih Village), Bondowoso (Bandilan Village), and Pamekasan (Pakong Village). This species has a potential to be utilized as a medicinal plant. Moreover, this plant grows wildly in all of the observed locations. Its presence needs to be considered since it is known as an invasive alien species in other regions.  Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1.5448 
INDEX AUTHOR AND SUBJECT VOL. 11 NO. 1 APRIL 2018 INDEX INDEX
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.623 KB) | DOI: 10.15408/kauniyah.v11i1.10038

Abstract

OPTIMASI FERMENTASI ASAM LAKTAT OLEH Lactobacillus casei PADA MEDIA FERMENTASI YANG DISUBTITUSI TEPUNG KULIT PISANG Fafa Nurdyansyah; Umar Hafidz Asyari Hasbullah
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.969 KB) | DOI: 10.15408/kauniyah.v11i1.6166

Abstract

Abstrak Tujuan dari penelitian ini adalah untuk menentukan kondisi optimum fermentasi asam laktat pada beberapa perbandingan konsentrasi substrat dan inokulum yang berasal dari pemanfaatan limbah kulit pisang sebagai subtrat dan Lactobacillus casei sebagai inokulum. Optimasi dilakukan dengan rancangan percobaan menggunakan Central Composite Design (CCD) dengan penentuan titik optimum variabel menggunakan Respon Surface Methodolgy (RSM). Tiga belas satuan percobaan, yaitu konsentrasi substrat (X1: 2,5; 5; 7,5% b/v) dan inokulum (X2: 2, 4, 6% v/v), digunakan untuk menentukan titik optimum proses fermentasi. Fermentasi dilakukan dengan sistem tumpak dengan volume media 100 mL dalam fermentor berupa labu Erlenmeyer 250 mL. Kultur diinkubasi pada goyangan 150 rpm dan suhu 37 °C, selama 72 jam. Selanjutnya, asam laktat hasil analisis digunakan sebagai respon penentuan titik optimum variabel proses fermentasi. Hasil penelitian menunjukkan bahwa rendemen proses penepungan kulit pisang yang dihasilkan adalah 23,02% dengan karakteristik kimia memiliki kadar pati, gulatotal, asam total, dan serat kasar secara berturut-turut adalah 5,35; 1,36; 0,61; dan 1,54 % (b/b). Titik optimum konsentrasi substrat dan inokulum, yaitu 4,71% dan 6,61%, menghasilkan konsentrasi biomassa yang optimum sebesar 5,99 g/L, sedangkan titik optimum variabel X1 dan X2 dengan konsentrasi substrat dan inokulum sebesar 4,31% dan 4,64% menghasilkan nilai optimum konsentrasi asam laktat sebesar 7,06 g/L, yang merupakan hasil produksi asam laktat terbaik pada penelitian ini.   Abstract The objective of this study is to determine the optimum condition of lactic acid fermentation with various concentrations of substrate and inoculum, which derived from the utilization of banana peel waste as substrate and Lactobacillus casei as inoculum. Response surface methodology (RSM) was used in this study. Variable optimization was conducted by Central Composite Design (CDC) with RSM to determine the optimum point of lactic acid fermentation variable. Those step used 13 groups with independent variable in the form of substrate concentrations (X1:2.5; 5; 7.5% w/v) and inoculum concentrations (X2: 2, 4, 6% v/v) to decide the optimum point of variable in the fermentation process. Fermentation was performed in a batch system with 100 mL media in a fermenter of 250 mL Erlenmeyer flask. The culture was incubated at 150 rpm and 37 °C for 72 hours.The result of lactic acid was used to determine the optimum point of variable in fermentation. The results showed that the yield from the fluor making process of banana peel was 23.02% with chemical characteristics of starch content, total sugar, total acid, and crude fiber were 5.35; 1.36; 0.61; and 1.54% w/w, respectively. The optimum concentration of substrate and inoculum concentration, 4.71% and 6.61%, resulted in optimum biomass concentration of 5.99 g/L, while the optimum point of X1 and X2 variables with substrate and inoculum concentration of 4.31% and 4.64% yielded optimum lactate acid concentration of 7.06 g/L, which is the best production of lactic acid in this study. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 6166  
MORFOLOGI PERMUKAAN DAUN TANAMAN TERUNG (Solanum melongena L.) SEBAGAI RESPONS TERHADAP CEKAMAN KEKERINGAN Ilahi, Rizky Nanda Kurnia; Isda, Mayta Novaliza; Rosmaina, Rosmaina
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.312 KB) | DOI: 10.15408/kauniyah.v11i1.5662

Abstract

Abstrak Terung (Solanum melongena L.) tergolong tanaman yang sensitif terhadap kekeringan selama tahap pertumbuhan dan perkembangannya. Karakteristik stomata dan trikoma merupakan kriteria yang dapat digunakan untuk mengidentifikasi tanaman yang toleran terhadap kekeringan. Penelitian bertujuan menguji respons karakteristik anatomi daun berupa trikoma dan stomata tanaman terung terhadap cekaman kekeringan melalui empat taraf interval penyiraman. Penelitian menggunakan Rancangan Acak Lengkap melalui empat taraf interval penyiraman, yakni 3, 6, 9, dan 12 hari.  Pengaruh cekaman kekeringan terhadap morfologi permukaan daun pada terung menyebabkan terjadinya peningkatan kerapatan trikoma tiga kali lebih banyak dibandingkan dengan kontrol, penurunan ukuran lebar trikoma mencapai 59,02%, penurunan ukuran lebar stomata mencapai 78,34%, dan penurunan ukuran lebar porus stomata mencapai 80,80%. Hasil penelitian menunjukkan bahwa peningkatan jumlah trikoma dengan ukuran trikoma yang semakin kecil diduga sebagai bentuk proteksi tanaman terhadap kerusakan jaringan dan mekanisme adaptasi tanaman untuk dapat memenuhi jumlah asimilasi CO2 perluas daun yang dibutuhkan untuk fotosintesis pada kondisi kekeringan. Sensitifitas tanaman terhadap kondisi kekeringan berupa mekanisme adaptasi dengan cara memperkecil ukuran stomata dan bukaan lebar porus, sehingga laju fotosintesis tetap terjaga pada kondisi kekeringandan mekanisme tanaman menjaga efisiensi penggunaan air dengan cara mengurangi ukuran stomata dan memperkecil bukaan porus stomata.    Abstract Eggplant (Solanum melongena L.) belongs to a group of plants that are sensitive to drought (water stress) during their growth and development stages. Characteristics of stomata and trichomes are criteria that can be used to identify drought-tolerant plants. This study aims to determine the response of leaf anatomical characteristics of the eggplant as well as trichome and stomata to drought stress through four levels of watering interval. The research using completely randomized design with watering intervals of 3, 6, 9, and 12 days. The effect of drought stress on leaf surface morphology of the eggplant resulted in three times greater trichomes density than control, decreased trichomes width by 59.02%, stomata width by 73.84%, and size of stomata porch width by 80.80%.. The result was showing that increasing number of trichome with smaller trichome size was thought to be a form of crop protection against tissue damage and plant adaptation mechanism in order to meet the amount of CO2 leaf expansion assimilation required for photosynthesis in drought stress condition. The sensitivity of plants to drought stress conditions is the mechanism of adaptation by reducing the size of stomata and wide porous opening, so that the rate of photosynthesis has been maintaining in the dry conditions and the mechanism of the plant maintain the efficiency of water use by reducing the size of stomata and minimizing stomata porous opening.   Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 5667
PEMBENTUKAN NODUL DARI BIJI MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS PADA MEDIA WPM DENGAN PENAMBAHAN BAP DAN MADU Hariono, Eko; Isda, Mayta Novaliza; Fatonah, Siti
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (753.258 KB) | DOI: 10.15408/kauniyah.v11i1.5422

Abstract

Abstrak Manggis (Garcinia mangostana L.) asal Bengkalis merupakan salah satu buah tropis yang menjadi komoditas ekspor Provinsi Riau dengan keunggulan dapat hidup di tanah gambut, tanah rawa, dan tanah masam. Pembentukan nodul tanaman manggis merupakan tahapan awal perbanyakan tunas pada kultur in vitro. Penelitian ini bertujuan untuk menentukan konsentrasi Benzilaminopurine (BAP) dan madu, baik tunggal maupun kombinasi, pada Woody Plant Medium (WPM) dalam pembentukan nodul dari eksplan biji manggis yang dibelah tiga secara membujur. Penelitian ini menggunakan Rancangan Acak Kelompok dengan menggunakan konsentrasi BAP 0, 5, dan 7 mg/L dan madu 0, 3, 6, dan 9 mL/L, baik tunggal maupun kombinasi. Hasil percobaan menunjukkan bahwa persentase pembentukan nodul paling tinggi (100%) diperoleh dari perlakuan 5 mg/L BAP. Perlakuan pemberian konsentrasi BAP dan madu, baik tunggal maupun kombinasi, pada media WPM, tidak mampu mempercepat waktu muncul nodul. Jumlah nodul terbanyak di 40 hari setelah tanam pada perlakuan 7 mg/L BAP yang disertai 3 mL/L madu adalah 25,0 nodul/biji.   Abstract Mangosteen (Garcinia mangostana L.) from Bengkalis in origin is one of the tropical fruit that became an export commodity of Riau Province, with the advantage of living in peat soil, swamp, and acidic soil. The formation of nodules is an early stage of shoot propagation in vitro cultures. This study aims to determine the concentration of BAP (Benzilaminopurine) and honey, either single or combination, in Woody Plant Medium (WPM) in the formation of nodules from mangosteen seed explants. This research employed Randomized Block Design to test the variation of BAP in the concentration of 0, 5, and 7 mg/L and honey in the concentration of 0, 3, 6, and 9 mL/L, either single or a combination. The results showed that the highest percentage of nodule formation (100%) was obtained from the treatment 5 mg/L of BAP. The treatment of BAP and honey, either single or combination, on WPM media, was unable to accelerate the timing of nodules. The highest number of nodules in 40 days after planting in the treatment of 7 mg/L of BAP with 3 mL/L of honey was 25.0 nodules/seed.  Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1.5422 
PENGARUH PENAMBAHAN BIOFLOK DENGAN DOSIS BERBEDA TERHADAP PERTUMBUHAN BENIH UDANG WINDU (Penaeus monodon FABRICIUS 1798) Ilham Zulfahmi; Muhammad Syahimi; Muliari Muliari
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.889 KB) | DOI: 10.15408/kauniyah.v11i1.4862

Abstract

AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh penambahan bioflok terhadap pertumbuhan dan kelangsungan benih udang windu. Penelitian dilakukan selama 30 hari dari Juni hingga Juli 2016 di Laboratorium Program Studi Budidaya Perairan Universitas Almuslim Bireuen. Wadah pemelihara-an berupa akuarium berukuran 50x30x40 cm3 bervolume 10 L dengan padat penebaran 15 ekor per wadah. Penelitian dilaksanakan secara eksperimental menggunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Perlakuan terdiri atas perlakuan A (kontrol), yaitu tanpa penambahan bioflok, perlakuan B, yaitu penambahan bioflok 10 mL dan 5% pakan komersil, perlakuan C, yaitu penambahan bioflok 15 mL dan 5% pakan komersil, dan perlakuan D, yaitu penambahan bioflok 15 mL dan tanpa penambahan pakan komersil. Parameter yang diamati pada akhir masa pemeliharaan meliputi laju pertumbuhan rata-rata spesifik harian, pertambahan panjang mutlak, dan kelangsungan hidup, yang kemudian diolah dengan menggunakan analisis variansi satu-faktor dengan taraf nyata 0,05. Hasil penelitian menunjukkan bahwa penambahan bioflok berpengaruh nyata terhadap laju pertumbuhan rata-rata spesifik harian dan pertambahan panjang mutlak. Perlakuan B menghasilkan laju pertumbuhan rata-rata spesifik harian tertinggi, yaitu 0,55±0,02% per hari, dan pertambahan panjang mutlak tertinggi, yaitu 3,7±0,1 cm. Namun demikian, penambahan bioflok tidak memberikan pengaruh yang nyata terhadap kelangsungan hidup benih udang windu.AbstractThis research aims to determine the influence of the addition of biofloc on the growth and survival rate of tiger shrimp juvenile. The research was carried out for 30 days from June to July 2016 in the aquaculture laboratory of Almuslim Bireuen University. The maintenance container used was an aquarium with a size of 50x30x40 cm3 that contains 10 L with stocking density 15 tiger shrimp juvenile per aquarium. The research was carried out experimentally using a complete randomized design with four treatments and three replicates. The treatments consist of treatment A (control) which has no addition of biofloc, treatment B which addition of 10 mL biofloc and 5% of commercial feed, treatment C which addition of 15 mL biofloc and 5% commercial feed, and treatment D which addition of 15 mL biofloc without commercial feed. The parameters observed at the end of the maintenance period include daily specific growth rate, absolute length increase, and survival, which were then analyzed by using a one-factor analysis of variance with a significance level of 0.05. The results showed that the addition of biofloc significantly influenced the daily specific growth rate and the absolute length increase. Treatment B gave the highest daily specific growth rate of 0.55±0,02% per day, and the highest absolute length increase of 3.7±0,1 cm. However, the addition of biofloc has no significant influence on the survival of tiger shrimp seeds.  Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 4862 
POTENSI JAMUR INDIGENUS RIAU (Penicillium sp.PN6) DAN Neptunia oleracea UNTUK BIOREMEDIASI OIL SLUDGE Wahyu Lestari; Atria Martina; Rodesia Mustika Roza; Imelda Wardani
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.863 KB) | DOI: 10.15408/kauniyah.v11i1.6350

Abstract

Abstrak Lumpur minyak bumi (oil sludge) memiliki kandungan kontaminan petroleum hidrokarbon terbesar, karenanya pengolahan limbah oil sludge perlu dilakukan agar tidak mencemari lingkungan. Penelitian yang menguji kemampuan jamur indigenus Riau (Penicillium sp. PN6) dan legum akuatik (Neptunia oleracea) dalam meremediasi senyawa hidrokarbon dengan pengukuran total petroleum hidrokarbon (TPH) pada limbah oil sludge telah dilakukan. Pertumbuhan N. oleracea dilakukan pada media limbah oil sludge dengan konsentrasi 25, 50, 75, dan 100%. Selanjutnya dilakukan pemberian inokulum Penicillium sp. PN6 ke dalam media pertumbuhan. Hasil penelitian menunjukkan bahwa persentase pertumbuhan N. oleracea adalah 95,58; 85,87; 25,55; dan 0%, berturut-turut pada limbah oil sludge 25, 50, 75, dan 100%. Terjadi penurunan konsentrasi TPH awal pada semua perlakuan di akhir pengamatan. Penicillium sp. PN6 meningkatkan degradasi TPH dari limbah oil sludge dibandingkan dengan kombinasi Penicillium sp. PN6 dengan N. oleracea, tetapi kombinasi Penicillium sp.PN6 dengan N. oleracea meningkatkan persentase penurunan TPH dari limbah oil sludge dibandingkan dengan menggunakan Penicillium sp. PN6 saja. Penicillium sp. PN6 dan kombinasinya dengan N. oleracea sama-sama mampu menurunkan pH limbah oil sludge.Abstract Oil sludge contains the largest contaminant of petroleum hydrocarbon that needs treatment to prevent its pollution into the environment. A study examining the ability of a Riau indigenous fungi (Penicillium sp. PN6) and an aquatic legume (Neptunia oleracea) to remediate hydrocarbon compounds by determining total petroleum hydrocarbon (TPH) in oil sludge waste has been conducted. The growth of N. oleracea was performed in the oil sludge waste media by concentrations of 25, 50, 75, and 100%. The medium was inoculated with Penicillium sp. PN6. The results showed that the percentage of growth of N. oleracea were 95.58; 85.87; 25.55; and 0%, in the oil sludge waste of 25, 50, 75, and 100%, respectively. There was a decrease in initial TPH concentration at all treatments at the end of the observation. Penicillium sp. PN6 increased more TPH degradation of the oil sludge waste compared to a combination of Penicillium sp. PN6 and N.oleracea, but the combination increased the decrease percentage of TPH compared to those that used Penicillium sp. PN6 only. Penicillium sp. PN6 and its combination with N. oleracea are both able to decrease pH of the oil sludge waste. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 6350 
ISOLASI DAN KERAGAMAN BAKTERI UREOLITIK LOKAL RIAU YANG BERPOTENSI SEBAGAI CAMPURAN BETON Mufidah Dwi Suci Ningsih; Tetty Marta Linda; Bernadeta Leni Fibriarti
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.771 KB) | DOI: 10.15408/kauniyah.v11i1.5737

Abstract

Abstrak Bakteri ureolitik merupakan mikroorganisme yang memiliki kemampuan untuk menghasilkan urease yang dapat mengendapkan kalsium karbonat (kalsit). Bakteri ini berpotensi sebagai agen bioremediasi logam berat dan sebagai bahan konstruksi beton. Penelitian ini bertujuan untuk mengisolasi, mengkarakterisasi, dan menguji presipitasi kalsit bakteri ureolitik dari tanah lokal Riau. Hasil penelitian memperoleh 30 isolat bakteri yang berhasil diisolasi dari tanah tempat pembuangan akhir, dan setelah dilakukan pewarnaan Gram, 77% isolat merupakan bakteri Gram positif dan hanya 33% merupakan bakteri Gram negatif.  Hasil uji presipitasi menunjukkan bahwa isolat- isolat dengan kode sp. 32, sp. 9, dan sp. 20 mampu membentuk kalsium karbonat berturut-turut sebesar 0,129 g, 0,126 g, dan 0,105 g, setelah diinkubasi selama 7 hari pada medium cair yang diberi penambahan urea dan kalsium. Isolat-isolat bakteri tersebut memiliki hubungan kekerabatan yang dekat, yang ditandai dengan besarnya koefisien kekerabatannya, yaitu lebih dari 70%. Dengan demikian, bakteri-bakteri yang terisolasi dan teruji dalam  membentuk kalsium karbonat asal tanah lokal Riau berasal dari sekelompok bakteri, yang berpotensi untuk dikembangkan sebagai campuran beton.  Abstract Ureolytic bacteria are microorganisms that have the ability to produce urease that precipitates calcium carbonate (calcite). This bacteria has potential as an agent for bioremediation of heavy metal and as a concrete construction material. The aim of this research is concerning about isolation, characterization, and examination on calcite precipitate of the ureolytic bacteria from Riau local soil. The result showed that 30 isolates were isolated from landfill soil, and after Gram staining, 77% of the isolates are Gram-positive and only 33% are Gram-negative. The result of precipitation examination showed that the bacterial isolates sp. 32, sp. 9, and sp. 20 precipitated 0.129 g, 0.126 g and 0.105 g of calcium carbonate, respectively, after incubation for 7 days in broth medium added with urea and calcium. The bacterial isolates have a close relationship, which is characterized by the magnitude of the coefficient of more than 70%. Therefore, the isolated and tested bacteria having the ability to form calcium carbonate from local soil Riau derived from a group of bacteria, which has a potential to be developed as a mixture of concrete. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 5737  

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