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INDONESIA
AL KAUNIYAH
Published by Universitas Islam Negeri Syarif Hidayatullah Jakarta
ISSN : 19783736     EISSN : 25026720     DOI : 10.15408/kauniyah
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology
Al-Kauniyah: Jurnal Biologi (p-ISSN: 1978-3736, e-ISSN: 2502-6720) is an Open Access Journal published by Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, and established since 2007. Since 2016 Al-Kauniyah has established a collaboration with the Association of Lecturer in Biology and Biology Education throughout the State Islamic Higher University (PTKIN) in Indonesia. Until 2015, Al-Kauniyah covered environmental biology solely, but since 2016 the journal has been extended to cover the entire field of biological science (bioscience). By publishing biannually, on April and October, Al-Kauniyah is intended to communicate original researches and current issues on the subject of biology. Since volume 9 issue 1 April 2016, Al-Kauniyah had been changes the layout. This journal warmly welcomes contributions from scholars of related disciplines. Manuscripts can be submitted to AL-KAUNIYAH
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Articles 299 Documents
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Peranan Jamur Rhizoctonia sp. Asal Taman Nasional Rawa Aopa Watumohai Sulawesi Tenggara terhadap Keberhasilan Aklimatisasi dan Laju Pertumbuhan Planlet Anggrek Macan (Grammatophyllum scriptum BL.) Rita Ningsih; Sri Ambardini; Denofia Denofia
Al-Kauniyah: Jurnal Biologi Vol 7, No 2 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.833 KB) | DOI: 10.15408/kauniyah.v7i2.2716

Abstract

The aim of this research was to know the role of Rhizoctonia sp. fungus from Taman Nasional Rawa Aopa Watumohai (TNRAW) to the successfully acclimatization and growth rate of tiger orchid planlet (Grammatophyllum scriptum Bl.) as well to know successfully infection of fungus Rhizoctonia sp. at the planlet root. The procedure of this research consists 3 main steps, they were fungus inoculation on orchid’s growing media, growth test and verification of successfully infection on planlet root. Growth test consisting of 3 treatments namely control (without nutrition and fungi) (K0); Rhizoctonia sp. application (R1); Rhizoctonia sp. + nutrition Potato Dextrose Borth (PDB) (R2) with the repetition for 8 times, then the growth data was analysed by statistics utilizes to F-test and continue by BNT-test on 95% trusty level. The result of the research showed that R2 treatment increase high planlet, leaf and root number. All the mean score of research indicator R2 was highest compared R1 and K0. The result of F-test showed R1 and R2 treatment gave significant influence toward the high planlet, leaf and root number growth. Except, fresh and dry weight planlet. Eventhough, fresh and dry weight was higher compared than K0 acclimatization period for a month.  Rhizoctonia sp. fungus infected planlet root through velamen to eksodermis and cortex by forming hifa scroll (pelotons).
KEANEKARAGAMAN FENOTIPIK NGENGAT (Subordo Heterocera) BERDASARKAN KARAKTER MORFOLOGI DI KAWASAN UNIVERSITAS RIAU DAN DESA SIABU, PROVINSI RIAU Ennie Chahyadi; Nuryani Nuryani; Dewi Indriyani Roslim
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1231.909 KB) | DOI: 10.15408/kauniyah.v12i1.6793

Abstract

AbstrakPenelitian tentang ngengat masih sangat sedikit dilakukan di Indonesia terutama di kawasan Universitas Riau dan Desa Siabu Kampar belum ada datanya. Penelitian ini bertujuan untuk menganalisis keanekaragaman fenotip ngengat berdasarkan karakter morfologi untuk mengetahui jarak genetik ngengat di kawasan Universitas Riau dan Desa Siabu. Penelitian dilakukan pada bulan Desember 2016 hingga Mei 2017. Lokasi pengambilan sampel terdapat di dua kawasan dengan enam lokasi yang berbeda yaitu Universitas Riau (Arboretum, Kebun FMIPA, Rusunawa) dan Desa Siabu (perumahan warga, Bukit Tentado dan hutan sekunder). Metode sampling mengunakan perangkap layar (light trap) sebagai sumber cahaya digunakan lampu mercuri merk Philips 160 watt. Bagian tubuh ngengat yang diamati adalah kepala, torak, abdomen dan sayap, jumlah karakter morfologi yang diamati adalah 24 karakter. Setiap karakter morfologi diberi skor kemudian dianalisis menggunakan program NTSYS ps versi 2.02i. Hasil yang didapat yaitu 61 spesies dengan 414 individu dari 10 famili ngengat.  Keanekaragaman fenetik ngengat di Universitas Riau dan Desa Siabu berturut turut adalah 87% dan 78%. Ngengat dari kedua kawasan menunjukkan perbedaan pada karakter ukuran, namun tidak pada karakter morfologi dan warna. Terdapat adanya kemiripan sebesar 66% antara ngengat yang dikoleksi dari Universitas Riau (kebun FMIPA, rusunawa) dengan Desa Siabu (Bukit Tentado dan hutan sekunder).Abstract Research on moths is still very few in Indonesia. Especially in the area of Riau University and Siabu Kampar Village. There is no datum about moths. The objective of this research is to analyze the genetic diversity of moths in Riau University and Siabu Vilage, Riau Province based on morphological characters. The study was conducted from December 2016 to May 2017. The moths were collected from two areas with six different locations such as Riau University (arboretum of mathematics and science faculty) and Siabu Village (village residence, Tentado Hill, and secondary forest). The sampling method that used was the light trap, with 160 watts of mercury, was used as a light source. The parts of the body observed were head, thorax, abdomen, and wings with the number of morphological characters observed by 24 characters. Morphological characters were observed and scored and then analyzed using the NTSYSp c version 2.02i software. The results showed that there were 61 species with 414 individuals from 10 families of moths. The genetic diversity of moth in the Riau University and Siabu Village was 87% and 78%. Moths on both locations indicated no difference in the shape and color, but they were different in size. Moths from FMIPA garden and rusunawa had 66% genetic similarity to one from Tentado Hill and secondary forest.
Optimalisasi Penggunaan Pupuk Kompos dengan Penambahan Effective Microorganism 10 (Em10) pada Produktivitas Tanaman Jagung (Zea mays L.) Elpawati Elpawati; Stephani Dwi Dara; Dasumiati Dasumiati
Al-Kauniyah: Jurnal Biologi Vol 8, No 2 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.662 KB) | DOI: 10.15408/kauniyah.v8i2.2693

Abstract

This research is aimed to determine the effect of growing media composition, the addition different concentration of EM10 fertilizer, and interaction between the composition of the growing media with different concentration of EM10 fertilizer on Zea mays growth and productivity. This research was conducted in Home Composting of UIN Syarif Hidayatullah Jakarta from June until October 2013. This study used Random Grouped for Factorial Design 2 x 5 wth 3 repetitions. First factor was plant media dose (M), consist of two extents, M1 (compost:soil = 1:1) and M2 (compost:soil = 1:2). The second factor was fertilizer concentration (D), consist of five extents, DO (no fertilizer),D1 (Urea: 0,9 g, SP-36: 0,9 g, KCl: 0,45 g), D2 (10 ml EM10), D3 (15 ml EM10), and D4 (20 ml EM10). The obtained data was analyzed using Analysis of Varians(ANOVA) and Duncan test when there is a significant difference..The result showed plant media (M2) with ratio composition of compost and soil was 1:2 could increase the stem diameter at harvest time. 20 ml concentration of EM10 could increase the stem diameter’s growth (2.29 mm) at harvest time, similarly 15 ml EM10 could increase the cob’s productivity (1.66 cobs). The interaction of composition plant media and fertilizer concentration of EM10 did not influence corn’s growth and productivity.
PERILAKU MAKAN MASYARAKAT PEDESAAN DI KALIMANTAN BARAT Wolly Candramila; Deska Erasmawanti; Desta Rotuana Sihombing; Hosniah Hosniah; Lisa Gola; Magdalena Magdalena
Al-Kauniyah: Jurnal Biologi Vol 11, No 2 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1090.713 KB) | DOI: 10.15408/kauniyah.v11i2.7615

Abstract

AbstrakKegiatan konversi lahan di Kalimantan Barat yang cenderung terus meningkat dapat berdampak negatif bagi ketersediaan pangan masyarakat desa yang masih melakukan food gathering dan berburu dari suplai alami di sekitar desa atau berternak dengan skala keluarga. Perilaku makan yang salah disertai lokasi desa yang relatif jauh dari fasilitas kesehatan yang lengkap turut menambah risiko kesehatan dan kematian pada masyarakat desa tersebut. Penelitian ini menjelaskan dan menentukan faktor-faktor yang mempengaruhi perilaku makan tiga etnis berbeda di Kalimantan Barat, mencakup Dayak, Madura dan Tionghoa, terkait penurunan ketersediaan lahan sebagai sumber pangan.  Survei dilakukan di empat kabupaten dan satu kota dengan sampel diambil secara acak dari populasi etnis Dayak (Kanayatn, Muduk, Ketungau Sesae’), Madura danTionghoa. Data perilaku makan dianalisis secara deskriptif mencakup frekuensi dan rutinitas makan, keragaman sumber makanan, estimasi pengeluaran energi harian dan klasifikasi aktivitas fisik sehari penuh, serta kondisi fisik sebagai manifestasi kegiatan makan dari 293 partisipan. Faktor-faktor yang mempengaruhi perilaku makan masyarakat pedesaan Kalimantan Barat adalah pekerjaan, keterlibatan individu dalam kelompok sosial, serta ketersediaan dan keterjangkauan sumber makanan. Konversi lahan hutan dan kebun akan mempengaruhi ketersediaan sumber makanan yang terjangkau termasuk perilaku makan sehat dan seimbang pada masyarakat pedesaan di Kalimantan Barat.AbstractMost rural communities in West Kalimantan were facing negative impact of increasing land conversion activities on their natural food supply. Improper eating behavior and limited health facilities may give more health and death risk to these people. This research described and determined the factors that influenced the eating behavior among three ethnics in West Kalimantan, namely Dayak, Madura and Chinese populations, and the potential impact of land conversion to it.  Survey was conducted in four regencies and one city and random sampling was done in three sub ethnics of Dayak (Muduk, Kanayatn, and Ketungau Sesae’), Madura, and Chinese communities. The eating behavior data was analyzed descriptively included meal frequency and routines, the variety of daily food source, individual estimates of energy expenditure, body mass index (BMI) distribution, and sports habit among 293 participants. Four main factors influenced the eating behavior of rural communities in West Kalimantan including types of occupation, individual involvement in social groups, and the availability and affordability of food source. The land conversion activity will certainly influence the affordable food source availability and the eating behavior for healthy and balanced food among the rural communities in West Kalimantan. 
POHON PELAWAN (Tristaniopsis merguensis): SPESIES KUNCI KEBERLANJUTAN HUTAN TAMAN KEANEKARAGAMAN HAYATI NAMANG – BANGKA TENGAH Dian Akbarini
Al-Kauniyah: Jurnal Biologi Vol 9, No 1 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.969 KB) | DOI: 10.15408/kauniyah.v9i1.3500

Abstract

Abstrak Pembangunan berkelanjutan merupakan pembangunan yang tidak memprioritaskan sektor ekonomi semata, tapi juga menyeimbangkan sektor sosial dan lingkungan. Salah satu indikator yang digunakan adalah kelimpahan keanekaragaman hayati yang terdapat di suatu wilayah. Indikator pembangunan berkelanjutan yang disusun oleh Perserikatan Bangsa Bangsa adalah kelimpahan spesies kunci. Pohon Pelawan (Tristaniopsis merguensis) merupakan satu spesies kunci bagi keberlanjutan keanekaragaman hayati di kabupaten Bangka Tengah. Pohon Pelawan dapat menjamin tetap tumbuhnya jamur Heimioporus sp. dan panen madu Pelawan di Taman Keanekaragaman Hayati kabupaten Bangka Tengah.Abstract Sustainable development is the development not only focusing on economic sectors but also balancing social and environment balance. One of the indicators used (in this research) is the abundance of biodiversity found in a region. Sustainable development indicators compiled by the United Nation is the abundance of key species. Pelawan tree (Tristaniopsis merguensis) is one of the key species for sustainable diversity in Central Bangka regency. Pelawan tree is supposed to preserve the growth of fungi Heimioporus sp. and the harvest of Pelawan honey in Biodiversity park, Central Bangka region. 
STRUKTUR KOMUNITAS ECHINODERMATA DI KAWASAN INTERTIDAL GILI MENO, LOMBOK UTARA M. Irsyad A. Ghafari; Gito Hadiprayitno; M. Liwa Ilhamdi; Noar Muda Satyawan
Al-Kauniyah: Jurnal Biologi Vol 12, No 2 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.31 KB) | DOI: 10.15408/kauniyah.v12i2.10871

Abstract

AbstrakEchinodermata merupakan salah satu kelompok invertebrata yang memegang peranan penting, baik secara ekologi maupun ekonomi. Pengetahuan tentang organisme ini sangat dibutuhkan terkait dengan keberadaannya di alam yang semakin tergerus oleh pembangunan dan aktivitas manusia yang tidak ramah lingkungan. Penelitian ini bertujuan untuk mengetahui struktur komunitas Echinodermata di kawasan intertidal Gili Meno, Lombok Utara, yang dilakukan pada bulan April 2018 di 6 stasiun pengamatan yang tersebar di kawasan intertidal. Pengambilan data dilakukan dengan metode belt transect dengan pengulangan sebanyak 2–4 kali pada setiap stasiun, dengan luas transek 100 m2. Hasil pengamatan menunjukkan bahwa ditemukan 13 spesies yang berasal dari 4 kelas Echinodermata, yaitu Asteroidea (1 spesies), Echinoidea (4 spesies), Holothuroidea (4 spesies), dan Ophiuroidea (4 spesies). Hasil penghitungan indeks ekologi menunjukkan bahwa indeks keanekaragaman komunitas Echinodermata berkisar antara 0,16–1,36 (kategori rendah-sedang). Indeks dominansi di setiap stasiun berkisar antara 0,32–0,94 (kategori rendah-sedang), sedangkan indeks kemerataan sebesar 0,25 (kategori rendah). Hal ini menunjukkan bahwa stabilitas komunitas Echinodermata di kawasan intertidal Gili Meno tergolong rendah. Hasil penelitian ini diharapkan dapat menjadi acuan dalam pembangunan kawasan wisata yang ramah lingkungan tanpa mengabaikan keberadaan sumber daya hayati laut di kawasan Gili Meno, Lombok Utara.Abstract Echinoderms are one group of invertebrates that play an essential role, both ecologically and economically. Knowledge about this organism is needed concerning its presence in nature, which increasingly eroded by the development and activities of people who are not environmental friendly. This study aims to determine the structure of the Echinoderms community in the intertidal area of Gili Meno, North Lombok, were conducted on April 2018 at six observation stations. Data were collected by belt transect method with repetition of 2–4 times at each station, with 100 m2 transect area.  The observations showed that found 13 species included in 4 classes of Echinoderms, namely Asteroidea (1 species), Echinoidea (4 species), Holothuroidea (4 species) and Ophiuroidea (4 species). The ecological index of the Echinoderms community showed that the diversity index ranged from 0.16–1.36 (low-medium). The dominance index at each station ranged from 0.32 to 0.94 (low-medium), while the evenness index was 0.25 (low). The stability of the Echinoderms community in Gili Meno intertidal area relatively low. The results expected to be a reference in the development of environmentally friendly tourism areas without ignoring the existence of living aquatic resources in the Gili Meno, North Lombok.
TERAPI GEN: DARI BIOTEKNOLOGI UNTUK KESEHATAN Dyah Ayu Widyastuti
Al-Kauniyah: Jurnal Biologi Vol 10, No 1 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.513 KB) | DOI: 10.15408/kauniyah.v10i1.4864

Abstract

Abstrak Rekayasa genetika dilakukannya untuk manipulasi gen-gen tertentu sehingga ekspresi gen dapat dikontrol dan produknya dapat dimanfaatkan untuk tujuan tertentu, salah satunya untuk pengobatan penyakit dengan terapi gen. Terapi ini melibatkan transfer asam nukleat berupa DNA ke sel embrionik maupun somatik pasien sehingga gen tersebut memiliki efek pengobatan terhadap penyakit pasien. Gen fungsional yang ditransfer berperan menggantikan fungsi gen abnormal yang menyebabkan penyakit tertentu. Terapi gen dapat dilakukan pada sel embrional (germ line gene therapy) maupun sel somatik (somatic cells gene therapy) pada pasien secara in vivo maupun ex vivo. Penyisipan gen pada terapi ini menggunakan vektor virus maupun non virus. Keberhasilan terapi gen salah satunya tergantung pada efektifitas transfer gen yang dilakukan dan ekspresi gen fungsional. Gen fungsional yang telah tertransfer selanjutnya harus diekspresikan dengan baik sehingga terapi dapat berhasil. Kanker merupakan salah satu penyakit yang memungkinkan dilakukannya terapi gen, terkait dengan adanya abnormalitas gen penyebab proliferasi sel yang tidak terkontrol. Abstract Genetic engineering has the ability to manipulate specific genes so its expression can be controlled. The control of gene expression can be used as a gene functional for appropriate purposes, such as diseases cure with gene therapy. This therapy involves DNA as nucleic acid which is transfer to embryonic or somatic cells of patients with certain diseases. The purpose of the gene therapy is to switch an abnormal gene to be a functional gene which has a cure effect for the disease. The functional gene has to substitute the abnormal gene leads to certain disease, either germ line gene therapy or somatic cells therapy. The gene therapy needs a vector to carry the functional gene to targeted cells, in vivo or ex vivo. The vector can be used viral or non viral vectors. The possibilities of achievement of its therapy depend on gene transfer effectiveness and also functional gene expression. The cancer is the one of diseases which can be treated with the gene therapy due to its uncontrolled gene of cell proliferation.
Pemanfaatan Plankton sebagai Sumber Makanan Ikan Bandeng di Waduk Ir. H. Juanda, Jawa Barat Deden Ibnu Aqil; Lily Surayya Eka Putri; Lukman Lukman
Al-Kauniyah: Jurnal Biologi Vol 6, No 1 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.509 KB) | DOI: 10.15408/al-kauniyah.v6i1.2826

Abstract

Introducing of Milkfish (Chanos chanos) was aimed to utilize natural food from plankton that were abundant in Ir. H. Juanda Reservoir, West Java. This research aimed to know plankton utilization by Milkfish in Ir. H. Juanda Reservoir, West Java. The research was conducted from February to July 2010 at 4 stations of sampling which representing the reservoir condition. The fishes collected based on the catch of fishermen. The sample was analyzed in physiology laboratory of Center Research for Limnology. The result of this research showed that the main food were plankton 43%, detritus 46%, and complement food were vegetation 11%. The domination food from the phytoplankton were Bacillariophyceae and zooplankton were Copepoda.
TUMBUHAN SEBAGAI OBAT TRADISONAL PASCA MELAHIRKAN OLEH SUKU ACEH DI KABUPATEN PIDIE Zumaidar Zumaidar; Saudah Saudah; Saida Rasnovi; Essy Harnelly
Al-Kauniyah: Jurnal Biologi Vol 12, No 2 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.974 KB) | DOI: 10.15408/kauniyah.v12i2.9991

Abstract

AbstrakPemanfaatan tumbuhan sebagai obat tradisional merupakan kearifan lokal yang diturunkan secara turun temurun berdasarkan resep nenek moyang, adat istiadat, kepercayaan atau kebiasaan setempat. Penelitian ini bertujuan untuk mengidentifikasi jenis tumbuhan yang digunakan dalam pengobatan pasca melahirkan oleh Suku Aceh di Kabupaten Pidie. Metode yang digunakan dalam pengumpulan data adalah teknik Participatory Rural Appraisal dan observasi. Parameter dalam penelitian ini adalah jenis tumbuhan obat, jenis ramuan obat dan cara penggunaan ramuan dalam pengobatan pasca melahirkan. Berdasarkan hasil penelitian diperoleh 25 jenis tumbuhan yang tergolong ke dalam 15 suku yang digunakan dalam pengobatan pasca melahirkan di Kabupaten Pidie. Jenis ramuan dalam pengobatan tradisional pasca melahirkan terdiri dari obat dalam dan obat luar. Obat dalam yang digunakan terdiri obat perut, bedak param, dan pilis. Penggunaan ramuan obat dalam dan obat luar selama pasca melahirkan dilakukan selama 44 hari. Manfaat dari penggunaan obat tersebut diantaranya menambah darah, meningkatkan jumlah air susu ibu, menghangatkan badan, dan menghilangkan lelah serta letih pasca melahirkan. Pemanfaatan tumbuhan obat secara tradisional sampai saat ini masih digunakan oleh masyarakat Aceh pada pengobatan ibu pasca melahirkan, selain pengobatan modern.Abstract Traditional medicine is a drug that is processed in a simple, hereditary based on ancestral recipes, customs, beliefs or local knowledge. This study aims to identify the species of plants used in post-natal care by Acehnese in Pidie District. The method used in data collection is Participatory Rural Appraisal and observation techniques. The parameters in this study are the species of medicinal plants, types of medicinal herbs and how to use the ingredients in postnatal care. The results of the study obtained 25 species plant that is grouped into 15 family used in post-natal care in Pidie District. A Traditional herbs post-natal medicine consists of internal and external medicine. The internal medicine used as stomach medicine, param powder and pilis. The use of medicinal herbs to internal and external medicine after postnatal was carried out for 44 days. The perceived benefits included adding blood, increasing the amount of breast milk, warming the body and eliminating fatigue and fatigue after childbirth. Utilization of traditional medicinal plants in this time are still used by Acehnese in the treatment of postnatal mother, besides modern treatment.
Kadar Protein Klebsiella pneumoniae Hasil Pemanasan 65 Derajat Celcius Irawan Sugoro; Devita Tetriana
Al-Kauniyah: Jurnal Biologi Vol 7, No 1 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.149 KB) | DOI: 10.15408/kauniyah.v7i1.2712

Abstract

Klebsiella pneumoniae is one of a coliform bacteria that causing mastitis. This disease were founded in dairy cows and can be prevented by vaccination. The research has been conducted to determine the inactive times, the protein concentration and profile of K. pneumoniae which inactivated by heating of 65oC as material of mastitis vaccine. The cells culture inactivated by the different times, i.e. 0, 10, 20, 30, 40, 50 and 60 minutes. The inactive times was determined by the drop test method, whereas the protein concentration of cells were determined by Lowry method. The results showed that the inactive times occured after 30 minute, and has a significant different on the protein concentration of bacteria cells that inactivated by the different times.

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